ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Microbial Physiology and Metabolism
Deep enrichment of soil Proteobacteria and its coupled response to carbon, nitrogen, and phosphorus cycles under quizalofop-p-ethyl stress
Provisionally accepted
Huan Meng
Yuanlong ChenLili YangYueyao LiZhiling NieJiayi CaoJianan DuChuanzhen MaYushuai WeiFengshan Yang*
Haiyan Fu*- Heilongjiang University, Harbin, China
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To investigate the vertical impacts of quizalofop-p-ethyl stress on soil bacterial communities and their ecological functional responses in wheat fields, this study collected soil samples from three depths (0–30 cm, 30–60 cm, and 60–90 cm) using a grid sampling method in typical wheat fields of Inner Mongolia Autonomous Region. Through quizalofop-p-ethyl acclimation experiments with concentration gradients (50–300 mg/L), combined with bacterial community structure and functional analyses, this study focused on revealing the dominant enrichment of Proteobacteria in deep soil and its key regulatory role in carbon (C), nitrogen (N), and phosphorus (P) cycles.The results showed that quizalofop-p-ethyl treatment significantly altered soil microbial community structure and induced obvious functional remodeling. As a core responsive taxon, the relative abundance of Proteobacteria increased significantly with increasing soil depth, becoming the absolute dominant phylum in deep soil layers. This change was significantly positively correlated with the upregulation of key metabolic pathways involved in soil C, N, and P cycles (including the citrate cycle, nitrogen metabolism, phosphonate metabolism, etc.). Functional gene analysis further indicated that the expression of multiple genes related to nitrogen assimilation and phosphorus utilization was closely associated with the abundance of Proteobacteria, directly promoting N and P cycling processes. Meanwhile, the activation of quizalofop-p-ethyl degradation-related pathways provided additional carbon sources for microorganisms, synergistically enhancing the C cycle.From the perspective of "dominant bacterial taxa driving element cycling," this study clarified the vertical differentiation mechanism of soil microbial ecological functions under quizalofop-p-ethyl stress, which deepens the understanding of the soil microecological effects of herbicides.
Keywords: carbon, nitrogen, and phosphoruscycles, Functional regulation, Herbicide degradation, Proteobacteria, quizalofop-p-ethyl, soil depth
Received: 13 Dec 2025; Accepted: 13 Feb 2026.
Copyright: © 2026 Meng, Chen, Yang, Li, Nie, Cao, Du, Ma, Wei, Yang and Fu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Fengshan Yang
Haiyan Fu
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