Your new experience awaits. Try the new design now and help us make it even better

ORIGINAL RESEARCH article

Front. Microbiol.

Sec. Extreme Microbiology

This article is part of the Research TopicBiology of ThermophilesView all articles

Genomic Analysis of an Extremophile PLS47 from Deep Sea-Vent

Provisionally accepted
Baiq Repika Nurul FurqanBaiq Repika Nurul Furqan1Made Puspasari WidhiastutyMade Puspasari Widhiastuty1Febriani FebrianiFebriani Febriani2Deviyanthi Nur AfifahDeviyanthi Nur Afifah1Teuku Mohamad IqbalsyahTeuku Mohamad Iqbalsyah2Akhmaloka AkhmalokaAkhmaloka Akhmaloka1*
  • 1Bandung Institute of Technology, Bandung, Indonesia
  • 2Universitas Syiah Kuala, Banda Aceh, Indonesia

The final, formatted version of the article will be published soon.

Select one of your emails

You have multiple emails registered with Frontiers:

Notify me on publication

Please enter your email address:

If you already have an account, please login

You don't have a Frontiers account ? You can register here

The PLS47 was isolated from deep-sea at Pria Laot Sabang hydrothermal Vent Site, was studied through whole-genome sequencing and annotation to explore gene functions and thermostable enzyme production. The complete genome sequence local isolate PLS47 is 3,772,236 bp at chromosomal genome with GC content at 52.02%, along with 56,806 bp at plasmid DNA with GC content at around 41.06%. A total of 3,708 coding sequences (CDSs) were identified, 6 rRNA (5S, 16S, and 23S), 27 rRNA, 88 tRNA genes, and 17 pseudogenes. A comparison of the genome to data based on Average Nucleotide Identity shows that the genome is closely related to the Geobacillus thermocatenulatus. Functional analysis revealed numerous enzyme-coding genes, including proteases, peroxidases hydrolases, esterases, dehydrogenase, hydratases, and lipases. In addition, the genome exhibits a number of stress-tolerant genes. Detailed analysis of the hydrolase genes, especially for lipolytic enzymes such as esterase and lipase, showed that the genome exhibits true lipase like putative lipase, monoacyl glycerol lipase (MAGL) motif and other lipase like GDSL-type esterase/lipase motif. The genomic information provides an understanding of thermophilic genomes and their relevance to stress-tolerant adaptation and explores potential genes, especially for industrial applications.

Keywords: deep-sea vent, extremophile, Genome, Geobacillus thermocatenulatus, PLS47

Received: 13 Dec 2025; Accepted: 26 Jan 2026.

Copyright: © 2026 Furqan, Widhiastuty, Febriani, Afifah, Iqbalsyah and Akhmaloka. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Akhmaloka Akhmaloka

Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Supplementary Material