Frontiers in Molecular Biosciences | Protein Biochemistry for Basic and Applied Sciences

Mini Review

Published on 06 Mar 2026

Capturing protein-protein interactions in plants: recent advances, challenges, and opportunities

in Protein Biochemistry for Basic and Applied Sciences

Berry Dickey
Yatendra Singh
Sibesh Maharjan
Yongjian Qiu
Sixue Chen

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2026.1777595

Original Research

Published on 26 Feb 2026

Neurofilament light chain proteins are a sensitive biomarker of neuronal damage in cirrhotic patients with hepatic encephalopathy

in Protein Biochemistry for Basic and Applied Sciences

Clelia Asero
Francesca Polito
Maria Stella Franzé
Antonio Battaglia
Claudia Ligresti
Teresa Maltese
Irene Cacciola
M’Hammed Aguennouz
Vincenzo Macaione

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2026.1744194

100 views

Original Research

Accepted on 16 Feb 2026

Optimization of protein extraction and digestion workflows for proteomic analysis of saliva, salivary stones and kidney stones

in Protein Biochemistry for Basic and Applied Sciences

Paulina Czaplewska
Natalia Musiał
Martyna Iwaniec
Inez Mruk
Michał Puchalski
Dmitry Tretiakow
Andrzej Skorek
Konrad Szydłowski
Jan Szlęzak

Frontiers in Molecular Biosciences

Methods

Published on 12 Feb 2026

Quantifying cytoskeletal protein interactions with far Western blotting

in Protein Biochemistry for Basic and Applied Sciences

Marcelo M. Melo
Elizabeth S. Phillippi
Mark L. Schultz
Susan Q. Shen
Hatem El-Shanti

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2026.1761723

546 views

Original Research

Accepted on 05 Feb 2026

Exploring hub genes related to adipocytokines in keloids: A combined analysis integrating single-cell, Mendelian randomization and bulk transcriptome data with experimental verification

in Protein Biochemistry for Basic and Applied Sciences

Maisude Mahemuti
Youyou Chen
Minxuan Li
Alimire Yilihamu

Frontiers in Molecular Biosciences

129 views

Original Research

Published on 29 Jan 2026

Biophysical insights into recombinant Zeocin binding protein: conformational stability and folding dynamics across pH and temperature

in Protein Biochemistry for Basic and Applied Sciences

Sara Alharbi
Ajamaluddin Malik
Abdulaziz Alamri
Javed Masood Khan
Mohd. Shahnawaz Khan
Abdullah Alhomida
Tauseef Ahmad

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2026.1748036

567 views

Review

Published on 23 Jan 2026

Advances in protein dot blot: principles, technical specifics, applications, and future perspectives

in Protein Biochemistry for Basic and Applied Sciences

Juan Lu
Feng-yi Mai
Xin-yu Li
Wen-tao Yang
Jing-rong Liang
Xing-long Li
Jie Guo
Chen-guang Li

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2026.1768231

1,303 views

Original Research

Accepted on 09 Jan 2026

Expanding the genetic code: phage-driven evolution of pyrrolysyl-synthetase for site-specific incorporation of synthetic phenylalanine and tyrosine derivatives

in Protein Biochemistry for Basic and Applied Sciences

Dakhnevich Anastasia
Sabina Miasoutova
Danila Iliushin
Aleksey Rozanov
Roman Ivanov

Frontiers in Molecular Biosciences

296 views

Original Research

Published on 09 Jan 2026

Isolation, characterization, and biological evaluation of endophytic fungi from Phragmites australis: experimental and computational insights

in Protein Biochemistry for Basic and Applied Sciences

Dina Mahfouz Eskander
Mohamed E. El Awady
Mohamed Ali
Asmaa M. Fahim
Ahmed A. Hamed
Basel Sitohy

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2025.1713876

1,107 views

Opinion

Published on 06 Jan 2026

Some aspects of trypsin’s involvement in the regulation of physiological functions

in Protein Biochemistry for Basic and Applied Sciences

I. V. Kuzmina
S. M. Tolpygo
V. G. Vertiprakhov

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2025.1750770

1,200 views

Original Research

Published on 18 Nov 2025

EIF3M as a pan-cancer biomarker: prognostic significance and immune infiltration association

in Protein Biochemistry for Basic and Applied Sciences

Zhilei Zhao
Jiaxing Chen
Yongqiang Cui
Zhihao Fu
Dongfeng Deng
Xiao Zhang

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2025.1697083

1,631 views

Editorial

Published on 13 Oct 2025

Editorial: Evolution, biochemistry and function of the dystroglycan-axis: from human diseases to model organisms

in Protein Biochemistry for Basic and Applied Sciences

Aaron M. Beedle
Mohammed Akaaboune
Bulmaro Cisneros
Andrea Brancaccio

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2025.1712184

1,161 views

Original Research

Published on 04 Sep 2025

The interaction of Cu(II) and Zn(II) with peptide fragment of HSPB1 and its analogs

in Protein Biochemistry for Basic and Applied Sciences

Agnieszka Szebesczyk
Domenica Musumeci
Ettore Napolitano
Halyna Kukhtenko
Paulina Iwaniak

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2025.1593661

2,312 views

Correction

Published on 18 Aug 2025

Correction: Anticarcinogenic cationic peptides derived from tryptic hydrolysis of β-lactoglobulin

in Protein Biochemistry for Basic and Applied Sciences

Eman Ibrahem
Ali Osman
Hefnawy Taha
Mohamed F. Abo El-Maati
Basel Sitohy
Mahmoud Sitohy

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2025.1660539

1,388 views

Opinion

Published on 14 Aug 2025

Integrating thermodynamics and molecular kinetics into western blotting teaching

in Protein Biochemistry for Basic and Applied Sciences

Zhiyong Wang
Min Wang
Yan Yu
Yanxin Lu
Qiang Xia
Pei Wei

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2025.1640740

2,488 views

Original Research

Published on 08 Aug 2025

In-silico evaluation of putative maternal semiochemicals of pigs with receptor proteins

in Protein Biochemistry for Basic and Applied Sciences

Devaraj Sankarganesh
Ambritha Balasundaram
Hayavadhan Sampath
Diya Manjunath
George Priya Doss C.

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2025.1600209

2,530 views

Editorial

Published on 14 Jul 2025

Editorial: Ironome: a still untapped frontier

in Protein Biochemistry for Basic and Applied Sciences

Gloria C. Ferreira
Vladimir N. Uversky
Zoubida Karim

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2025.1651154

2,396 views

Original Research

Published on 10 Jul 2025

Structural bioinformatics and gene expression analysis of maturase K from Lavandula angustifolia (lavender)

in Protein Biochemistry for Basic and Applied Sciences

Dafeng Liu
Na Li
Daoqi Song
Zhenming Lv

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2025.1628118

1,904 views
2 citations

Original Research

Published on 30 May 2025

Flexible iron: disorder in the ironome brings order to protein structure and function

in Protein Biochemistry for Basic and Applied Sciences

Vladimir N. Uversky
Gloria C. Ferreira

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2025.1537164

3,691 views
1 citation

Editorial

Published on 22 May 2025

Editorial: Emerging advancements in the carbonic anhydrase field

in Protein Biochemistry for Basic and Applied Sciences

Simona Maria Monti
Vincenzo Alterio
Seppo Parkkila
Claudiu T. Supuran
Giuseppina De Simone

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2025.1624394

2,042 views

Methods

Published on 13 May 2025

Thioflavin T in-gel staining for ex vivo analysis of cardiac amyloid

in Protein Biochemistry for Basic and Applied Sciences

Joseph Oldam
Irina Tchernyshyov
Jennifer Van Eyk
Juan Troncoso
Charles G. Glabe
Giulio Agnetti

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2025.1505250

2,543 views

Original Research

Published on 09 Apr 2025

Positional distribution and conservation of major phosphorylated sites in the human kinome

in Protein Biochemistry for Basic and Applied Sciences

Athira Perunelly Gopalakrishnan
Prathik Basthikoppa Shivamurthy
Mukhtar Ahmed
Samseera Ummar
Poornima Ramesh
Sonet Daniel Thomas
Althaf Mahin
Mahammad Nisar
Sowmya Soman
Yashwanth Subbannayya

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2025.1557835

3,390 views
8 citations

Original Research

Published on 07 Apr 2025

Influence of expression and purification protocols on Gα biochemical activity: kinetics of plant and mammalian G protein cycles

in Protein Biochemistry for Basic and Applied Sciences

Timothy E. Gookin
David Chakravorty
Sarah M. Assmann

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2025.1513660

2,288 views
1 citation

Original Research

Published on 03 Apr 2025

Live cell optical super-resolution microscopy of dystroglycan mutants as a model for dystroglycanopathies in multiple cell lines

in Protein Biochemistry for Basic and Applied Sciences

Francesca Sciandra
Manuela Bozzi
Alina Witt
Paul Goffing
Sonia Covaceuszach
Sandra Blaess
Alberto Cassetta
Maria Giulia Bigotti
Thomas Huser
Andrea Brancaccio

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2025.1558170

2,318 views

285 articles

articles

Capturing protein-protein interactions in plants: recent advances, challenges, and opportunities

Neurofilament light chain proteins are a sensitive biomarker of neuronal damage in cirrhotic patients with hepatic encephalopathy

Optimization of protein extraction and digestion workflows for proteomic analysis of saliva, salivary stones and kidney stones

Quantifying cytoskeletal protein interactions with far Western blotting

Exploring hub genes related to adipocytokines in keloids: A combined analysis integrating single-cell, Mendelian randomization and bulk transcriptome data with experimental verification

Biophysical insights into recombinant Zeocin binding protein: conformational stability and folding dynamics across pH and temperature

Advances in protein dot blot: principles, technical specifics, applications, and future perspectives

Expanding the genetic code: phage-driven evolution of pyrrolysyl-synthetase for site-specific incorporation of synthetic phenylalanine and tyrosine derivatives

Isolation, characterization, and biological evaluation of endophytic fungi from Phragmites australis: experimental and computational insights

Some aspects of trypsin’s involvement in the regulation of physiological functions

EIF3M as a pan-cancer biomarker: prognostic significance and immune infiltration association

Editorial: Evolution, biochemistry and function of the dystroglycan-axis: from human diseases to model organisms

The interaction of Cu(II) and Zn(II) with peptide fragment of HSPB1 and its analogs

Correction: Anticarcinogenic cationic peptides derived from tryptic hydrolysis of β-lactoglobulin

Integrating thermodynamics and molecular kinetics into western blotting teaching

In-silico evaluation of putative maternal semiochemicals of pigs with receptor proteins

Editorial: Ironome: a still untapped frontier

Structural bioinformatics and gene expression analysis of maturase K from Lavandula angustifolia (lavender)

Flexible iron: disorder in the ironome brings order to protein structure and function

Editorial: Emerging advancements in the carbonic anhydrase field

Thioflavin T in-gel staining for ex vivo analysis of cardiac amyloid

Positional distribution and conservation of major phosphorylated sites in the human kinome

Influence of expression and purification protocols on Gα biochemical activity: kinetics of plant and mammalian G protein cycles

Live cell optical super-resolution microscopy of dystroglycan mutants as a model for dystroglycanopathies in multiple cell lines