Frontiers in Molecular Biosciences | Protein Biochemistry for Basic and Applied Sciences

Original Research

Published on 29 Jan 2026

Biophysical insights into recombinant Zeocin binding protein: conformational stability and folding dynamics across pH and temperature

in Protein Biochemistry for Basic and Applied Sciences

Sara Alharbi
Ajamaluddin Malik
Abdulaziz Alamri
Javed Masood Khan
Mohd. Shahnawaz Khan
Abdullah Alhomida
Tauseef Ahmad

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2026.1748036

299 views

Review

Published on 23 Jan 2026

Advances in protein dot blot: principles, technical specifics, applications, and future perspectives

in Protein Biochemistry for Basic and Applied Sciences

Juan Lu
Feng-yi Mai
Xin-yu Li
Wen-tao Yang
Jing-rong Liang
Xing-long Li
Jie Guo
Chen-guang Li

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2026.1768231

409 views

Original Research

Accepted on 22 Jan 2026

Deciphering Skeletal Muscle Trait Differences Between Chinese a nd Western Pig Breeds Based on 4D-label Ubiquitin-Proteomics

in Protein Biochemistry for Basic and Applied Sciences

Yunlei Li
Zikai AI
Yun Zhang
Mingfu Xiong
Jingyang DONG
Yilong YAO
Yaxing LIU
Jian LI
Yongjin ZHANG
Xinhao FAN

Frontiers in Molecular Biosciences

126 views

Methods

Accepted on 13 Jan 2026

Quantifying cytoskeletal protein interactions with Far Western Blotting

in Protein Biochemistry for Basic and Applied Sciences

Marcelo Melo
Elizabeth Phillippi
Mark Schultz
Susan Shen
Hatem El-Shanti

Frontiers in Molecular Biosciences

147 views

Original Research

Accepted on 09 Jan 2026

Expanding the genetic code: phage-driven evolution of pyrrolysyl-synthetase for site-specific incorporation of synthetic phenylalanine and tyrosine derivatives

in Protein Biochemistry for Basic and Applied Sciences

Dakhnevich Anastasia
Sabina Miasoutova
Danila Iliushin
Aleksey Rozanov
Roman Ivanov

Frontiers in Molecular Biosciences

235 views

Original Research

Published on 09 Jan 2026

Isolation, characterization, and biological evaluation of endophytic fungi from Phragmites australis: experimental and computational insights

in Protein Biochemistry for Basic and Applied Sciences

Dina Mahfouz Eskander
Mohamed E. El Awady
Mohamed Ali
Asmaa M. Fahim
Ahmed A. Hamed
Basel Sitohy

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2025.1713876

932 views

Opinion

Published on 06 Jan 2026

Some aspects of trypsin’s involvement in the regulation of physiological functions

in Protein Biochemistry for Basic and Applied Sciences

I. V. Kuzmina
S. M. Tolpygo
V. G. Vertiprakhov

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2025.1750770

1,044 views

Original Research

Published on 18 Nov 2025

EIF3M as a pan-cancer biomarker: prognostic significance and immune infiltration association

in Protein Biochemistry for Basic and Applied Sciences

Zhilei Zhao
Jiaxing Chen
Yongqiang Cui
Zhihao Fu
Dongfeng Deng
Xiao Zhang

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2025.1697083

1,563 views

Editorial

Published on 13 Oct 2025

Editorial: Evolution, biochemistry and function of the dystroglycan-axis: from human diseases to model organisms

in Protein Biochemistry for Basic and Applied Sciences

Aaron M. Beedle
Mohammed Akaaboune
Bulmaro Cisneros
Andrea Brancaccio

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2025.1712184

1,088 views

Original Research

Published on 04 Sep 2025

The interaction of Cu(II) and Zn(II) with peptide fragment of HSPB1 and its analogs

in Protein Biochemistry for Basic and Applied Sciences

Agnieszka Szebesczyk
Domenica Musumeci
Ettore Napolitano
Halyna Kukhtenko
Paulina Iwaniak

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2025.1593661

2,210 views

Correction

Published on 18 Aug 2025

Correction: Anticarcinogenic cationic peptides derived from tryptic hydrolysis of β-lactoglobulin

in Protein Biochemistry for Basic and Applied Sciences

Eman Ibrahem
Ali Osman
Hefnawy Taha
Mohamed F. Abo El-Maati
Basel Sitohy
Mahmoud Sitohy

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2025.1660539

1,323 views

Opinion

Published on 14 Aug 2025

Integrating thermodynamics and molecular kinetics into western blotting teaching

in Protein Biochemistry for Basic and Applied Sciences

Zhiyong Wang
Min Wang
Yan Yu
Yanxin Lu
Qiang Xia
Pei Wei

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2025.1640740

2,313 views

Original Research

Published on 08 Aug 2025

In-silico evaluation of putative maternal semiochemicals of pigs with receptor proteins

in Protein Biochemistry for Basic and Applied Sciences

Devaraj Sankarganesh
Ambritha Balasundaram
Hayavadhan Sampath
Diya Manjunath
George Priya Doss C.

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2025.1600209

2,395 views

Editorial

Published on 14 Jul 2025

Editorial: Ironome: a still untapped frontier

in Protein Biochemistry for Basic and Applied Sciences

Gloria C. Ferreira
Vladimir N. Uversky
Zoubida Karim

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2025.1651154

2,288 views

Original Research

Published on 10 Jul 2025

Structural bioinformatics and gene expression analysis of maturase K from Lavandula angustifolia (lavender)

in Protein Biochemistry for Basic and Applied Sciences

Dafeng Liu
Na Li
Daoqi Song
Zhenming Lv

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2025.1628118

1,829 views
2 citations

Original Research

Published on 30 May 2025

Flexible iron: disorder in the ironome brings order to protein structure and function

in Protein Biochemistry for Basic and Applied Sciences

Vladimir N. Uversky
Gloria C. Ferreira

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2025.1537164

3,449 views
1 citation

Editorial

Published on 22 May 2025

Editorial: Emerging advancements in the carbonic anhydrase field

in Protein Biochemistry for Basic and Applied Sciences

Simona Maria Monti
Vincenzo Alterio
Seppo Parkkila
Claudiu T. Supuran
Giuseppina De Simone

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2025.1624394

1,974 views

Methods

Published on 13 May 2025

Thioflavin T in-gel staining for ex vivo analysis of cardiac amyloid

in Protein Biochemistry for Basic and Applied Sciences

Joseph Oldam
Irina Tchernyshyov
Jennifer Van Eyk
Juan Troncoso
Charles G. Glabe
Giulio Agnetti

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2025.1505250

2,430 views

Original Research

Published on 09 Apr 2025

Positional distribution and conservation of major phosphorylated sites in the human kinome

in Protein Biochemistry for Basic and Applied Sciences

Athira Perunelly Gopalakrishnan
Prathik Basthikoppa Shivamurthy
Mukhtar Ahmed
Samseera Ummar
Poornima Ramesh
Sonet Daniel Thomas
Althaf Mahin
Mahammad Nisar
Sowmya Soman
Yashwanth Subbannayya

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2025.1557835

3,272 views
4 citations

Original Research

Published on 07 Apr 2025

Influence of expression and purification protocols on Gα biochemical activity: kinetics of plant and mammalian G protein cycles

in Protein Biochemistry for Basic and Applied Sciences

Timothy E. Gookin
David Chakravorty
Sarah M. Assmann

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2025.1513660

2,061 views
1 citation

Original Research

Published on 03 Apr 2025

Live cell optical super-resolution microscopy of dystroglycan mutants as a model for dystroglycanopathies in multiple cell lines

in Protein Biochemistry for Basic and Applied Sciences

Francesca Sciandra
Manuela Bozzi
Alina Witt
Paul Goffing
Sonia Covaceuszach
Sandra Blaess
Alberto Cassetta
Maria Giulia Bigotti
Thomas Huser
Andrea Brancaccio

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2025.1558170

2,229 views

Mini Review

Published on 25 Mar 2025

How to quantify magic spots - a brief overview of (p)ppGpp detection and quantitation methods

in Protein Biochemistry for Basic and Applied Sciences

Filip Gąsior
Wiktoria Klasa
Katarzyna Potrykus

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2025.1574135

4,129 views
1 citation

Original Research

Published on 18 Mar 2025

Identification of a neuron-specific ferroptosis in the neurodegenerative mucopolysaccharidosis III model

in Protein Biochemistry for Basic and Applied Sciences

Mathilde Larribau
Myriam Rouahi
Christophe Santiago
Jérôme Ausseil
Zoubida Karim

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2025.1476513

3,770 views

Review

Published on 20 Feb 2025

Two sides of the same coin: heat shock proteins as biomarkers and therapeutic targets for some complex diseases

in Protein Biochemistry for Basic and Applied Sciences

Xolani Henry Makhoba

Frontiers in Molecular Biosciences

doi 10.3389/fmolb.2025.1491227

5,407 views
6 citations

282 articles

articles

Biophysical insights into recombinant Zeocin binding protein: conformational stability and folding dynamics across pH and temperature

Advances in protein dot blot: principles, technical specifics, applications, and future perspectives

Deciphering Skeletal Muscle Trait Differences Between Chinese a nd Western Pig Breeds Based on 4D-label Ubiquitin-Proteomics

Quantifying cytoskeletal protein interactions with Far Western Blotting

Expanding the genetic code: phage-driven evolution of pyrrolysyl-synthetase for site-specific incorporation of synthetic phenylalanine and tyrosine derivatives

Isolation, characterization, and biological evaluation of endophytic fungi from Phragmites australis: experimental and computational insights

Some aspects of trypsin’s involvement in the regulation of physiological functions

EIF3M as a pan-cancer biomarker: prognostic significance and immune infiltration association

Editorial: Evolution, biochemistry and function of the dystroglycan-axis: from human diseases to model organisms

The interaction of Cu(II) and Zn(II) with peptide fragment of HSPB1 and its analogs

Correction: Anticarcinogenic cationic peptides derived from tryptic hydrolysis of β-lactoglobulin

Integrating thermodynamics and molecular kinetics into western blotting teaching

In-silico evaluation of putative maternal semiochemicals of pigs with receptor proteins

Editorial: Ironome: a still untapped frontier

Structural bioinformatics and gene expression analysis of maturase K from Lavandula angustifolia (lavender)

Flexible iron: disorder in the ironome brings order to protein structure and function

Editorial: Emerging advancements in the carbonic anhydrase field

Thioflavin T in-gel staining for ex vivo analysis of cardiac amyloid

Positional distribution and conservation of major phosphorylated sites in the human kinome

Influence of expression and purification protocols on Gα biochemical activity: kinetics of plant and mammalian G protein cycles

Live cell optical super-resolution microscopy of dystroglycan mutants as a model for dystroglycanopathies in multiple cell lines

How to quantify magic spots - a brief overview of (p)ppGpp detection and quantitation methods

Identification of a neuron-specific ferroptosis in the neurodegenerative mucopolysaccharidosis III model

Two sides of the same coin: heat shock proteins as biomarkers and therapeutic targets for some complex diseases