ORIGINAL RESEARCH article
Front. Oncol.
Sec. Cancer Molecular Targets and Therapeutics
Volume 15 - 2025 | doi: 10.3389/fonc.2025.1579282
This article is part of the Research TopicUnlocking Autophagy’s Full Potential: Embracing a Multidimensional Approach for Targeted Cancer TreatmentView all 7 articles
CD46 TREM1 regulates the autophagy marker LC3B ATG5 in oral squamous cell carcinoma
Provisionally accepted
- 1Binzhou Medical University, Binzhou, Shandong Province, China
- 2Qingdao Stomatological Hospital, Qingdao, Shandong Province, China
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This study aims to explore the regulatory effect of abnormal expression of CD46 and TREM1 on LC3B and ATG5 and to provide new targets for the molecular mechanism and treatment of OSCC. Human oral squamous cell carcinoma cells CAL-27 were selected for in vitro culture. For related research, the CD46 shRNA interference test, immunohistochemistry (IHC), flow cytometry, qRT-PCR detection, Western blot, and tumor-bearing animal models were used. In vitro cell experiments showed that CD46 and TREM1 were highly expressed on the surface of OSCC cells, while the expression of LC3B and ATG5 was significantly decreased. Compared with the control group (SC-shRNA), the CD46 shRNA group could effectively reduce the expression of CD46 in OSCC cells and increase the expression of autophagy and apoptosis protein (P < 0.01).In vivo experiments showed that the tumor volume of the shRNA group was significantly smaller than that of the SC-shRNA group (P < 0.01), the expression of CD46 and TREM1 was decreased considerably, and the expression of LC3B and ATG5 was higher (P < 0.01). Conclusion OSCC cells have high expression of CD46 and TREM1, while low expression of LC3B and ATG5, and the autophagy apoptosis signal is weakened. Interfering with CD46 can up-regulate the expression of autophagy apoptosis genes, reduce the tumor inflammatory microenvironment, induce the apoptosis of OSCC cells, and inhibit the proliferation and metastasis of OSCC. This study provides a new idea for the mechanism and targeted therapy of OSCC and has important theoretical significance and clinical application value.
Keywords: Squamous cell carcinoma, CD46/TREM1, Regulation mechanism, LC3B, Atg5
Received: 19 Feb 2025; Accepted: 28 Apr 2025.
Copyright: © 2025 Liu, GAO, Wang, Lv, Cong and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Beibei Cong, Qingdao Stomatological Hospital, Qingdao, 266001, Shandong Province, China
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