ORIGINAL RESEARCH article
Front. Oncol.
Sec. Head and Neck Cancer
Volume 15 - 2025 | doi: 10.3389/fonc.2025.1585277
This article is part of the Research TopicTargeting Tumor Microenvironment in Oral Cancer: Mechanisms and Therapeutic PotentialView all 3 articles
SPINK1 facilitates tumor progression via the EGFR/JAK/STAT3 axis in oral squamous cell carcinoma: Insights from single-cell RNA sequencing
Provisionally accepted
- 1Central South University, Changsha, China
- 2Xiangya School of Stomatology, Central South University, Changsha, Hunan Province, China
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Objective:This study aimed to elucidate the functional role and molecular mechanisms of Serine Peptidase Inhibitor Kazal Type 1 (SPINK1) in oral squamous cell carcinoma (OSCC) through integrative analysis of single-cell RNA sequencing (scRNA-seq) data.Materials and Methods: Cellular subpopulations within OSCC were stratified using transcriptomic datasets from the GEO database. Cell-cell communication networks were reconstructed to map ligand-receptor interactions, while Gene Set Variation Analysis (GSVA) and Gene Set Enrichment Analysis (GSEA) were employed to systematically investigate SPINK1-associated signaling pathways. SPINK1 expression profiles in OSCC tissues were validated through quantitative PCR (qPCR) and immunoblotting. Gain-and loss-of-function assays utilizing Cell Counting Kit-8 (CCK-8), wound healing assays, transwell migration/invasion chambers, and murine xenograft models were implemented to assess SPINK1-mediated oncogenic phenotypes.Rescue experiments conclusively established the EGFR/JAK/STAT3 signaling axis as the mechanistic backbone of SPINK1-driven oncogenesis. Results: SPINK1 was closely associated with T cells, malignant cells, and an array of immune modulators, including chemokines and immunoinhibitors, throughout OSCC progression. SPINK1 operates through pathways involving JAK/STAT3, P53, Notch and WNT signaling cascades. Relative to their normal tissue counterparts, SPINK1 is upregulated in OSCC, resulting in increased cell proliferation, invasion, and migration upon SPINK1 overexpression, whereas SPINK1 knockdown has opposite effects. SPINK1 knockdown led to a significant reduction in EGFR and STAT3 phosphorylation levels, whereas exogenous supplementation of EGFR effectively rescued this phenotype. Conclusion: SPINK1 has been established as a novel therapeutic target in OSCC, with its dual role in tumorigenesis and immune modulation providing a molecular foundation for developing targeted therapeutic modalities and precision oncology strategies.
Keywords: SPINK1, OSCC, single-cell RNA sequencing, EGFR, JAK/STAT3 Signaling pathways
Received: 28 Feb 2025; Accepted: 30 Jul 2025.
Copyright: © 2025 Bao and Tang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Zhan Gui Tang, Central South University, Changsha, China
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