ORIGINAL RESEARCH article
Front. Oncol.
Sec. Breast Cancer
Volume 15 - 2025 | doi: 10.3389/fonc.2025.1585945
This article is part of the Research TopicNovel Approaches to Overcome Drug Resistance in Breast CancerView all 3 articles
ESCO2 Inhibition Induces Cell Cycle Arrest and Apoptosis in Breast Cancer via the P53-CDK1 Axis and the BAX/Bcl2/Caspase Signaling Cascade
Provisionally accepted
- Guangxi Medical University Cancer Hospital, Nanning, China
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Background: Breast cancer is a major threat to women's health, and dysregulation of the cell cycle is a critical driver of its progression. ESCO2, a potential key regulator of the cell cycle, is implicated in cancer development; however, its specific role and mechanisms in breast cancer remain poorly understood.We analyzed differentially expressed genes between breast cancer and normal breast samples from GEO datasets to identify potential key regulators of the cell cycle pathway. ESCO2 expression was further investigated in breast cancer cell lines. Functional assays, including overexpression and knockdown of ESCO2 in MDA-MB-231 and MDA-MB-468 cells, were performed to assess its effects on the cell cycle and apoptosis. Molecular mechanisms were explored using Western blot, and rescue experiments were conducted to validate key regulatory pathways.Results: Analysis of the GSE38959 and GSE70947 datasets identified 541 common differentially expressed genes, with 26 genes enriched in the cell cycle pathway. ESCO2 interacted with multiple cell cycle-related genes and was significantly overexpressed in breast cancer. Overexpression of ESCO2 promoted DNA replication, while its knockdown induced G2/M phase arrest via the ESCO2-P53-CDK1 regulatory axis, and triggered apoptosis through the BAX/Bcl2/caspase9/caspase7 signaling cascade. The effects of ESCO2 knockdown on the cell cycle and apoptosis were rescued by siP53.Our findings reveal that ESCO2 is upregulated in breast cancer and may contribute to cell cycle regulation and apoptosis through the p53-CDK1 and BAX/Bcl-2-caspase pathways. These results highlight ESCO2 as a potential therapeutic target and provide new mechanistic insights into breast cancer progression.
Keywords: ESCO2, breast cancer, Cell Cycle, Apoptosis, p53
Received: 01 Mar 2025; Accepted: 26 Jun 2025.
Copyright: © 2025 Li, Wei, Li, Liu and Yang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Huawei Yang, Guangxi Medical University Cancer Hospital, Nanning, China
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