ORIGINAL RESEARCH article
Front. Oncol.
Sec. Gastrointestinal Cancers: Hepato Pancreatic Biliary Cancers
Volume 15 - 2025 | doi: 10.3389/fonc.2025.1611872
Significantly higher incidence of circulating fatty objects in portal blood samples compared to peripheral blood samples in patients with pancreatic ductal adenocarcinoma
Provisionally accepted
Ruoxiang Wang1*
Kenneth Park1
Srinivas Gaddam1
Quin Liu1Rabindra Watson1
Adrian Lim1Yan Ou2
Yi Zhang2
Mouad Edderkaoui1
Michael Lewis3Simon K Lo1
Stephen J Pandol1- 1Department of Medicine, Cedars Sinai Medical Center, Los Angeles, United States
- 2Department of Biomedical Sciences, Cedars Sinai Medical Center, Los Angeles, California, United States
- 3Department of Pathology, Cedars-Sinai Medical Center, Los Angeles, United States
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We previously reported the identification of circulating fatty objects (CFOs), abnormal entities found in peripheral blood samples from cancer patients. CFOs are large spherical objects with high contents of heavy cholesterol lipids, posing a risk of vessel embolization and circulation occlusion, common complications in clinical cancers. Initial characterization suggested that CFOs bear resemblance to bile salts, prompting further investigation into the potential link between bile and cancer-associated vascular occlusion. To explore this connection, we analyzed portal blood samples from patients diagnosed with pancreatic ductal adenocarcinoma (PDAC), as CFOs were most frequently detected in this type of cancer (20.4% incidence). Portal vein samples were collected via transhepatic endoscopic ultrasound-guided fine-needle aspiration and treated with ammonium chloride hemolysis to facilitate enumeration and characterization of CFOs in the nucleated blood cell fraction. CFOs were observed in 14 out of the 16 portal samples, indicating a significantly higher incidence compared to peripheral samples. Despite this, portal CFOs exhibited similar characteristics to peripheral CFOs. In a parallel study, a portion of the portal samples was filtered through an Exthera's Seraph 100 column before hemolysis, resulting in no detection of CFOs. These findings suggest that CFOs originate from bile. It is probable that CFOs are insoluble bile lipids that have ebbed into the portal vein and subsequently shunted to the systemic circulation due to PDAC-induced hepatobiliary system abnormalities. It appears that hemofiltration can effectively remove CFOs from circulation.
Keywords: circulating fatty object, portal vein blood, Peripheral Blood, Hemofiltration, and pancreatic ductal adenocarcinoma
Received: 15 Apr 2025; Accepted: 14 Aug 2025.
Copyright: © 2025 Wang, Park, Gaddam, Liu, Watson, Lim, Ou, Zhang, Edderkaoui, Lewis, Lo and Pandol. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Ruoxiang Wang, Department of Medicine, Cedars Sinai Medical Center, Los Angeles, United States
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