Abstract
Phyllodes tumor (PT) of the breast is a rare fibroepithelial tumor characterized by the proliferation of both epithelial and stromal components. The presence of osteosarcomatous differentiation within the sarcomatous stroma is exceptionally uncommon and typically portends a poor prognosis. However, the biological behavior of malignant phyllodes tumors (MPT) exhibiting heterologous osteosarcomatous differentiation requires further investigation. A 59-year-old woman presented with a one-month history of a left breast lump. Mammography, chest CT, ultrasonography and MRI identified a mass measuring 3.7cm×5.6cm×4.7cm. Notably, the time-intensity curve derived from DCE-MRI demonstrated a pattern of rapid initial enhancement followed by slow washout. The patient underwent wide local excision, and postoperative histopathology examination confirmed MPT with heterologous osteosarcomatous differentiation, predominantly composed of neoplastic bone. The patient declined adjuvant therapy and was managed with regular follow-up. Twelve months later, she returned with a recurrent breast mass. Mammography and chest CT showed a calcified mass measuring 6.3cm×6.5cm, resembling the previous lesion. A total mastectomy accompanied by partial resection of the pectoral major muscle was performed. The histopathological examination of the second specimen was consistent with the initial diagnosis. Following the second surgery, the patient received four cycles of chemotherapy and was maintained on regular surveillance. Ten months later, follow-up CT imaging revealed extensive pleural effusion with complete passive atelectasis of the left lung, along with scattered patchy and curvilinear calcifications along the mediastinal and parietal pleura. The patient declined further chemotherapy and opted for traditional Chinese medicine, and she died three months later. MPT of the breast with heterologous osteosarcomatous differentiation is an exceedingly rare entity with a poor prognosis despite aggressive therapeutic interventions. Different from previously reported cases, our case elucidates the tumor’s biological behavior through serial image follow-up, and highlights its hypervascularity which was not detected by color Doppler ultrasound but was clearly demonstrated on DCE-MRI.
Introduction
Phyllodes tumor (PT) of the breast is a rare fibroepithelial neoplasm marked by the proliferation of both epithelial and stromal components. Originating from the intralobular and periductal stroma, PT pathogenesis involves complex epithelial-stromal interactions. Molecular analyses of both components have revealed coordinated alterations in signaling networks that drive tumorigenesis. Key mutations within the stromal compartment propel its overgrowth and malignant progression, definitively distinguishing PT from fibroadenoma (, ). Accounting for only 0.3%-1.0% of all primary breast tumors, PT exhibits a higher incidence among Asian populations compared to Western cohorts (). Based on histopathological features, PT is categorized into benign, borderline, and malignant with corresponding recurrence rates of 10%-17%, 14%-25%, and 23%-30%, respectively (). PT typically manifests as a unilateral, large (often >10cm), circumscribed breast mass. While sonography, mammography, CT, and MRI may reveal a lobulated mass, occasionally with high calcified attenuation, but these imaging characteristics contribute limited value in predicting tumor grade. Diagnosis and grading rely mainly on histopathological evaluation. As reported in the literature, 10%-15% of phyllodes tumors are malignant (). According to the World Health Organization(WHO) Classification of Breast Tumors (2019), diagnostic criteria for malignant phyllodes tumor (MPT) include marked stromal nuclear pleomorphism, stromal overgrowth, high mitoses (≥5 mitoses/mm2), increased stromal cellularity, and an infiltrative border. In even rarer instances, sarcomatous stromal elements within PT may encompass angiosarcoma, chondrosarcoma, leiomyosarcoma, osteosarcoma, and rhabdomyosarcoma, frequently indicative of an adverse clinical outcome. Herein, we report a case of breast MPT comprised predominantly of heterologous osteosarcoma, systemically reviewing its clinical presentation, imaging features, pathological characteristics, and disease course. Additionally, we performed a comprehensive literature review via PubMed for scattered case reports of breast PT with osteosarcomatous differentiation published since 2000. By analyzing these collective data, we aim to advance the understanding of this disease entity to facilitate accurate diagnosis and optimal management.
Case presentation
A 59-year-old female complaining of a left breast lump for one month was admitted to the Department of Breast Surgery on December 14, 2020. She had been postmenopausal for 12 years and denied any history of hormone therapy. She first noticed a nodule in her left breast 1 month prior which had progressively enlarged over the preceding 3 weeks. Physical examination revealed a 5.0cm×4.0cm firm, movable mass in the lower inner quadrant of the left breast, with no associated skin changes. The axillary lymph nodes and contralateral breast were normal. Mammography showed a hyperdense mass with lobulated borders (Breast Imaging Reporting and Data System BI-RADS 3, indicating probably benign) (Figure 1A). Ultrasonography revealed a solid mass without obvious internal vascularity on color Doppler (BI-RADS 4, indicating suspicious for malignancy). Chest CT (Figure 1B) showed a lobulated hyperdense mass with diffuse calcification, and the left thorax was normal, with no evidence of chest wall invasion aside from displacement of the adjacent pectoralis major muscle. MRI demonstrated a well-defined and lobulated lump measuring 3.7cm×5.6cm×4.7cm. The tumor exhibited iso- or hypointense signal intensity to normal breast tissue on fat-suppressed T1-weighted images (Figure 1C). Fat-suppressed T2-weighted images showed areas of mild hyperintensity and hypointensity, surrounded by a rim of high signal intensity (Figure 1D). Dynamic contrast-enhanced MRI(DCE-MRI) revealed heterogeneous enhancement (Figure 1E), with a time-intensity curve indicative of rapid enhancement and slow washout (BI-RADS MR 5, highly suggestive of malignancy) (Figure 1F). Cerebral and abdominal CT scans showed no evidence of distant metastasis. Given the extensive calcification observed on mammography and CT, obtaining an adequate specimen for pathological diagnosis via needle biopsy was challenging. Due to the patient’s concern over potential tumor dissemination during biopsy, she declined this procedure. Consequently, excisional biopsy was pursued as an alternative for definitive diagnosis.
Figure 1
The patient underwent wide local excision on December 17, 2020. Intraoperatively, the mass was found not to involve the thoracic wall muscles. The resected tumor measured 5.5cm×4.0cm×3.5cm and exhibited a bony gross appearance. Microscopically, the osteogenic tumor consisted primarily of well-differentiated bone trabeculae fusing with abundant microvasculatures and interspersed benign glandular structures (Figures 2A, B). Adjacent to the trabeculae, sarcomatoid cells displayed significant atypia and hypercellularity. The tumor border was circumscribed and infiltrative. Immunohistochemical analysis showed that tumor cells were negative for pan-cytokeratin (CKpan), while benign ductal epithelium was focally positive. Tumor cells expressed SATB2 and SMA but were negative for P63. The Ki-67 reached 40% in the hotspot area. CD34 staining presented microvascular plexus within the bone trabeculae (Figure 2C). After thorough examination of the tumor bed, a benign epithelial component was identified, confirming the final diagnosis of MPT with heterologous osteosarcomatous differentiation. The patient declined adjuvant chemotherapy and radiotherapy at that time.
Figure 2
On November 19, 2021, the patient returned with a recurrent mass in the left breast. Four months ago, she noticed a nodule in the left breast, then the nodule progressively enlarged. Physical examination revealed an 8.0cm×5.0cm firm mass with ill-defined borders. The axillary lymph nodes and contralateral breast remained unremarkable. Mammography showed a 6.3cm×6.5cm calcified mass (BI-RADS 3) (Figure 3A). There was no evidence of distant metastasis on cerebral, thoracic (Figure 3B), and abdominal CT. Based on clinical presentation and previous history, a diagnosis of tumor recurrence was made, and radical surgical intervention was recommended following multidisciplinary discussion. On November 25, 2021, the patient underwent total mastectomy and partial excision of the pectoralis major muscle. Gross appearance showed an 8.0cm×8.0cm×7.5cm bone-like tumor situated beneath the nipple adjacent to the superficial fascia. The microscopic morphology of the recurrent tumor resembled that of the initial specimen. Pathological diagnosis confirmed recurrent MPT with heterologous osteosarcomatous differentiation. Postoperatively, the patient received four cycles of chemotherapy and was regularly monitored.
Figure 3
On October 15, 2022, surveillance CT showed a large amount of pleural effusion with complete passive atelectasis of the left lung, accompanied by patchy and curvilinear calcifications along the mediastinal and parietal pleura (Figures 3C, D), suggestive of extensive pleural metastases. Given the clinical and imaging findings, a diagnosis of distant metastasis was made, and she commenced traditional medicine therapy.
In January 2023, the patient was deceased at the last follow-up. Figure 4 outlines the timeline of the major events in this case.
Figure 4
Discussion
As a rare entity, heterologous sarcomatous differentiation of MPT encompasses liposarcoma (excluding well-differentiated subtypes), osteosarcoma, chondrosarcoma, fibrosarcoma, or rhabdomyosarcoma component. A survey of 213 pathologists from 29 countries indicated that only 170 had encountered heterologous elements in MPT during their practice, with the incidence as follows: liposarcoma (53/170, 31.2%), chondrosarcoma (49/170, 28.8%), osteosarcoma (31/170, 18.2%), and rhabdomyosarcoma (17/170, 10.0%) (). In English literature, most reports are isolated case with limited data. The clinical presentation and course of patients vary depending on the type of heterologous element. In 1999, Silver & Tavasolli reviewed the clinicopathological features and outcomes of 22 cases of MPT with osteosarcomatous differentiation (). They reported a mean patient age of 60y (range: 40y-83y) and a mean tumor size of 6.4cm (range:1.9cm-19cm). Half of the tumors were grossly circumscribed and lobulated. Histologically, osteosarcomatous elements were categorized as fibroblastic (50%, featuring spindled malignant cells arranged in a storiform pattern), osteoclastic (27%, abundant non-neoplastic giant cells), or osteoblastic (23%, predominantly neoplastic bone matrix). Heterologous components constituted 25% to 100% of the tumor area. Notably, 43% of the reported patients experienced recurrence or metastasis and died within 12 months. Their analysis revealed that tumor size greater than 5cm and/or histological subtype of osteoclastic/osteoblastic were closely related to poor prognosis.
Since 2000, including the present case, 27 cases have been reported in the English literature (Table 1). In summary, the mean patient age was 53.2y (range:24y-76y). Tumors occurred in the left breast in 14 cases (52%) and the right breast in 13 (48%). The mean tumor size was 8.8cm (range:3cm-22.8cm, from 26 surgical cases, one autopsy case without documented tumor size).Ten patients (37%) died from tumor recurrence within 2.5 to 40 months post-surgery, among these, one patient died of recurrence without recorded time after initial surgery. Two patients (7%) were alive with recurrent tumor at last follow-up (5 and 36 months, respectively), while 11 (41%) were disease-free at last follow-up (ranging from 6 to 85 months). Four patients (15%) were lost to follow-up.
Table 1
| Authors | Year | Age | Site | Size (cm) | Image manifestations | Treatment | Final pathology | Follow up |
|---|---|---|---|---|---|---|---|---|
| Jha et al. () | 2023 | 32 | L | 10 | US: irregularly shaped hypoechoic lesion with partially circumscribed and microlobulated margins | Total mastectomy | Osteosarcoma(30%) with an osteoblastic component originating from MPT (70%) | NA |
| Ko () | 2023 | 52 | R | 7 | Mammography: high-dense mass with coarse and amorphous macro- and microcalcifications US: heterogeneous solid and cystic mass MRI: irregular-shaped multi-cystic complex mass with a predominantly cystic appearance and peri-lesional edema | Modified radical mastectomy with axillary dissection without further therapy such as radiation or chemotherapy | MPT with heterologous osteosarcoma and chondrosarcomatous differentiation | 8 months after surgery, stable |
| Ali et al. () | 2023 | 51 | R | 14.9 | US: a lobulated mass with heterogeneous enhancement, abutting the pectoralis muscle | Total mastectomy followed by radiotherapy | MPT with mixed osteosarcomatous and rhabdomyosarcomatous elements | 6 months, no recurrence or metastasis |
| Bhandari et al. () | 2023 | 55 | R | NA | Autopsy record | Mastectomy | MPT showing foci of osteosarcomatous differentiation | No time or image documented, died from widespread metastasis of gastrointestinal tract |
| Laforga et al. () | 2020 | 56 | L | 16 | US: tumor occupying the entire breast | Radical mastectomy and subsequent chemotherapy | MPT with heterologous osteosarcomatous differentiation and aneurysmatic bone cyst-like features | 31 months, died from pleural effusion and multiple lung metastases |
| Wu et al. () | 2020 | 58 | R | 15 | MRI: breast occupation CT: recurrence on the chest wall 2.5 months later | Expanded resection, radiotherapy and apatinib treatment | MPT with heterologous chondro- and osteosarcomatous dedifferentiation | 2.5 months, multiple bone metastases on PET-CT. 6 months, died, suspicious of coexistent brain metastasis |
| Berkesoglu et al. () | 2020 | 55 | R | 15 | PET-CT: protruding lesion from the breast | Mastectomy and chemoradiotherapy | MPT with osteosarcomatous dedifferentiation | 40 months, died from metastatic lesion |
| Patel et al. () | 2019 | 45 | R | 22.8 | US: lobulated mass of mixed echogenicity with areas of necrosis | Total mastectomy and chemoradiotherapy | MPT with osteosarcomatous dedifferentiation | Three years later, metastases to the skull, another 49 months after treatment, disease-free |
| Tokoyoda et al. () | 2018 | 52 | L | 7 | CT: a nodular calcified mass | Surgery and following postoperative radiotherapy | MPT with dominant osteosarcomatous differentiation | 1 year, died from metastasis to the heart |
| Sarkar et al. () | 2016 | 24 | R | 5 | NA | Simple mastectomy with following radical mastectomy and chemotherapy | MPT with heterologous chondro- and osteosarcomatous dedifferentiation | Five months later, recurrence and dissemination of subcutaneous nodules, left mediastinal mass, and a necrotic axillary node on CT |
| Patil Okaly et al. () | 2015 | 40 | L | 5 | NA | Simple mastectomy | MPT with heterologous chondro- and osteosarcomatous dedifferentiation | 1 year, disease-free |
| Warrier et al. () | 2015 | 50 | L | 11 | Mammogram and ultrasound: occupied lesion | Mastectomy and radiotherapy | MPT with heterologous chondro- and osteosarcomatous dedifferentiation and DCIS | 2 years, disease-free |
| Warrier et al. () | 2015 | 53 | L | 3.3 | US: solid mass with a heterogeneous echo pattern, cystic spaces and well-defined margins. | Wide local excision | MPT with heterologous lipo- and osteosarcomatous dedifferentiation | 2 years, disease-free |
| Mačák et al. () | 2014 | 71 | R | 10 | NA | Mastectomy | MPT with osteosarcomatous differentiation | 3 years, died from right ventricular apex metastasis and circulatory failure |
| Phalak et al. () | 2013 | 63 | L | 7 | Mammogram: a lobulated high-dense mass with partially circumscribed, partially obscured margins and associated coarse heterogeneous calcifications US: a round hypoechoic mass with associated vascularity and multiple strong echogenicity | Wide excision following adjuvant chemotherapy | MPT with osteosarcomatous differentiation | 10 months after the operation, pleural metastases |
| Singhal et al. () | 2011 | 40 | L | 6 | Mammogram: a well-defined mass with lobulated margins and areas of calcification similar to bone, fine eggshell calcification around the tumor | Simple mastectomy | MPT with heterologous chondro- and osteosarcomatous dedifferentiation | 5 years, disease-free |
| Reisenbichler et al. () | 2009 | 55 | L | 14.5 | NA | Modified radical mastectomy | MPT with osteosarcomatous differentiation | 6 months, disease-free |
| Tomas et al. () | 2007 | 71 | R | 3.3 | NA | Radical mastectomy and chemotherapy | MPT with osteo-, chondro- and liposarcomatous differentiation | 1 year, disease-free |
| Ribeiro-Silva et al. () | 2006 | 49 | R | 8 | NA | Radical mastectomy | MP with osteosarcomatous differentiation | Two months after the mastectomy, recurrence. 8 months after surgery, metastasized to the liver, brain, lungs, and skin of the right forearm. 1 year later, died |
| Sando et al. () | 2006 | 49 | R | 12 | NA | Modified radical mastectomy | MPT with osteosarcomatous differentiation | Nine months after surgery died from multiple pulmonary metastases |
| Choudhary et al. () | 2006 | 76 | R | 4 | Mammogram: a well-defined lobulated mass with dense calcification. US: difficult to interpret vascularity because of the calcification producing significant acoustic shadowing | Total mastectomy | MPT with osteosarcomatous differentiation | Increased uptake around the right hip and medial compartment of both knees and a focal spot of intense uptake overlying the right anterior chest wall on the bone scan. NA |
| Bhartia et al. () | 2005 | 45 | L | 3 | Mammogram and US: a soft tissue mass with coarse macrocalcification | Simple mastectomy and subsequent chemoradiotherapy | MPT with osteosarcomatous differentiation | 19 months, died from persistent right pleural effusion secondary to pleural metastases, nodules in the lung with increasing calcification on CT |
| Mukherjee et al. () | 2004 | 51 | R | 6 | Mammography: an asymmetrical density with scattered microcalcifications. US: a heterogenous mass with solid and cystic components and evidence of increased vascularity | Total mastectomy and chemotherapy | MPT with osteosarcomatous differentiation | NA |
| Tsubochi et al. () | 2004 | 54 | L | 8 | Mammography: soft mass without calcification | subcutaneous mastectomy | MPT with osteosarcomatous differentiation | One year after mastectomy, bilateral pulmonary tumors with calcification (metastasis), another 2 years after lung surgery, disease-free |
| Fischer et al. () | 2003 | 66 | L | 3.9 | Mammogram: a radiodense area with calcifications. US: a complex mass with irregular margins, with significant shadowing indicating the presence of calcium | Lumpectomy | MPT with osteosarcomatous differentiation | NA |
| Matsuo et al. () | 2001 | 64 | L | 5 | Mammography: an irregular tumorous lesion with coarse calcifications | surgery | MPT with osteosarcomatous differentiation | 6 months, disease-free |
| Present case | 59 | L | 5.5 | Detailed data in this manuscript | Surgery and chemotherapy | MPT with osteosarcomatous differentiation | 25 months, Died of metastasis |
Case Summary of breast malignant phyllodes tumor with osteosarcomatous differentiation since 2000 (including present case).
R, right; L, left; MPT, malignant phyllodes tumor; US, ultrasonography; CT, computed tomography; MRI, magnetic resonance imaging; DICS, ductal carcinoma in situ; NA, not available.
According to the cases listed in Table 1, imaging examinations revealed calcification in 10 tumors (, , , , –, , ), and only 2 cases had vascular signals within the tumors (, ). Among recurrent tumors, two cases exhibited calcification on follow-up imaging (, ). Of the metastatic cases, pleural metastasis was observed in 3 patients (, , ), pulmonary metastasis in 2 (, ), cardiac metastasis in 2 (, ), bone and brain metastasis in 1 (), skull metastasis in 1 (), joint metastasis in 1 (), and widespread metastasis in 3 (, , ); one case had metastasis with no documented location (). Among the 9 reported cases with calcification on image, 2 died from the disease, 2 developed metastasis, 4 were alive with follow-up period shorted than 8 months, and only 1 remained disease-free at 5 years (, , , , –, , ). The case with both calcification and internal vascularity spread to diffuse pleura within ten months after surgery (). Including our present case, we speculate that the coexistence of calcification and increased vascularity may be a high-risk factor for recurrence, warranting more aggressive medical intervention. More young patients with larger tumors have been recorded in the literature since 2000. Advances in treatment may have contributed to improved overall survival and prolonged disease-free periods. However, the limited reported cases and lack of original data precluded a detailed statistical analysis. Further accumulation of well-documented cases is needed to identify clinically and pathologically significant prognostic factors.
Based on the WHO 2019 diagnostic criteria, the diagnosis of MPT is very strict. A definitive pathological diagnosis requires the presence of all characteristic morphological features, including marked stromal nuclear pleomorphism, stromal overgrowth, high mitoses (≥5 mitoses/mm2), increased stromal cellularity and an infiltrative border. However, the presence of malignant heterologous elements-such as liposarcoma (excluding well-differentiated liposarcoma), osteosarcoma, chondrosarcoma, fibrosarcoma, or rhabdomyosarcoma-allows for a diagnosis of MPT with heterologous differentiation, irrespective of whether the conventional histopathological criteria are fully met.
Microscopic patterns of our case including primary and recurrent neoplasm, are mainly composed of osteosarcomatous neoplastic bone. The foci of benign epithelial components confirmed the biphasic nature of the tumor. Abundant microvasculature was observed among bone trabeculae. Heterologous components constituted nearly 100% of the tumor tissue in both lesions. These pathological features help clarify the initially perplexing imaging findings: significant calcified attenuation on mammography and CT limited the acquisition of additional diagnostic information, even with contrast-enhanced CT. Conversely, the enhancement pattern on contrast-enhanced MRI provided valuable clues regarding malignancy. The bony matrix attenuated ultrasound wave, and the blood flow signals were unable to be detected on colored Doppler ultrasound. Our patient experienced disease progression despite aggressive treatments. This is in keeping with the dismal prognosis of the neoplastic bone-forming type of the heterologous element in the literature ().
The main differential diagnosis for MPT is metaplastic carcinoma. The essential diagnostic criterion is the absence of a conventional infiltrating carcinoma component with mesenchymal differentiation. In our case, we did find small foci of CKpan-positive epithelial elements in the CKpan-negative osteosarcoma areas, but the epithelial element exhibited its benign feature with a low Ki-67 index and no obvious mitosis. So, the metaplastic carcinoma was ruled out. Other differentials include primary or metastatic osteosarcoma of the breast. The incidence of primary breast osteosarcoma varies greatly. According to the data from the Armed Force Institute of Pathology (AFIP), only 50 cases were reported between 1957 and 1995 (). However, there was only 1 breast osteosarcoma in the database of Mayo Clinic from 1910 to 2000 (, ) and 3 cases in M.D Anderson Cancer Center from 1947 to 1990 (). These discrepancies likely reflect evolving diagnostic criteria over time. According to WHO 2019 guidelines, a definitive diagnosis requires thorough sampling to exclude any epithelial component and to rule out metastatic tumor based on clinical history. Thus, meticulous identification of benign epithelial elements is vital to differentiate MPT with osteosarcomatous components from primary breast osteosarcoma.
The marked vascularization within the neoplastic bone is a hallmark of the present case. However, conventional ultrasound was unable to visualize these intratumoral vessels due to obscuration by the surrounding bony matrix. Contrast-enhanced MRI proved to be a more appropriate modality to identify the intrinsic nature of the tumor. Alternative diagnostic procedures such as DCE-MRI should be ordered when ultrasound findings are inconclusive. In breast carcinoma, time-intensity curves are characterized by rapid uptake and washout, often accompanied by focal calcifications. However, the tumor in our patient showed rapid uptake and slow washout with diffuse calcification. This point may be a meaningful diagnostic indicator and influence therapeutic decision. It has been reported that high microvessel density (MVD) correlates with a good response to chemotherapy in osteosarcoma (). Moreover, novel treatment strategies such as nanoparticle-based targeting of tumor vasculature are under development (). Therefore, accurate assessment of the vascular patterns within the tumor is important for oncologists to make an effective treatment plan.
Complete resection of the tumor remains the primary treatment for breast MPT. Following the initial extensive local excision, the patient experienced fatigue and expressed concern regarding potential side effects of chemotherapy and radiotherapy. The lack of adjuvant treatment post-surgery contributed to rapid recurrence and accelerated disease progression. Clinicians should ensure patients are fully aware of the aggressive characteristics of the tumor and take more radical measures to control the disease. The communication between the physician and the patient is crucial and an experienced psychologist may help the treatment plan proceed smoothly. Actually, after the first recurrence, the patient acknowledged the malignancy’s severity and consented to chemotherapy. Nonetheless, this did not hinder the progression of the disease. The recurrence of the disease is indicative of a poor prognosis. It has been shown that both chemotherapy and radiotherapy have limited efficacy against sarcomatous components. Provided the patient’s condition permits following comprehensive evaluation, more radical resection may still be of value. All reported cases in the literature underwent surgery to remove the primary tumor. After the surgery, 6 received chemotherapy, 3 received radiotherapy, 3 received concurrent chemoradiotherapy, and 1 received radiotherapy combined with targeted therapy. Although the treatment data are limited, total removal of the primary tumor with adjuvant chemoradiotherapy has been associated with improved overall survival. According to our literature review on MPT since 2000, rates of recurrence and metastasis have declined markedly, and the overall survival has significantly increased. Recent studies suggest that nanoparticles with high affinity for tumor vasculature may enhance ultrasonic signals and facilitate earlier detection. Novel treatment targeting the tumor microvasculature may reduce the chemo-resistance of the sarcoma and improve the overall survival. For advanced disease, such innovative strategies may be considered within clinical trials (). Furthermore, different bioactive nanoparticles can deliver drug more precisely, overcome biological barriers, amplify anticancer signaling pathways, remodel the immune microenviroment, and modulate osteogenic catalytic processes, et al. ().
Preoperative needle biopsy has become a standard clinical practice in the diagnosis of breast tumors, as it allows pathologists to provide essential information on tumor type and biomarker status. However, as also noted in the NCCN guidelines (V5.2024), needle biopsy is not always a definitive diagnostic option. In such cases, excision biopsy serves as an alternative option for accurate pathological classification. According to the same NCCN guidelines, radical surgery, total axillary lymph node dissection, and adjuvant chemotherapy are not routinely recommended for malignant phyllodes tumors. However, our present case and previous literature showed that the clinical behavior varies significantly across different histological subtypes. In aggressive subtype, such as heterologous osteosarmotous differentiation, more extensive surgical resection and aggressive systemic therapy should be administered. Preoperative imaging, particularly DCE-MRI could effectively evaluate the vascular pattern masked by the diffuse calcification, which portends the rapid growth and aggressive biological behavior. Postoperative histopathological examination is of importance to identify the high-risk subtypes. Finally, communication between the oncologist and the patient about the prognosis and individualized treatment strategies is crucial for optimal clinical management.
Conclusion
MPT with heterologous osteosarcomatous differentiation is a rare disease entity with a dismal prognosis, even when managed aggressively. Unlike other reported cases, the present case was characterized by heterologous osteosarcomatous components constituting nearly the entire tumor volume; moreover, its pathological basis-particularly the rich vascularity- was detected only through DCE-MRI. Intrathoracic metastases exhibited imaging features similar to the primary tumor. We should integrate various information of comprehensive imaging and meticulous pathological examination to make a definitive diagnosis. Aggressive treatment and vigilant monitoring measures are essential to improve outcomes in this rare disease.
Statements
Data availability statement
The original contributions presented in the study are included in the article/supplementary material. Further inquiries can be directed to the corresponding author.
Ethics statement
Written informed consent was obtained from the patient’s next of kin for the publication of any potentially identifiable images or data included in this article.
Author contributions
LG: Writing – original draft, Data curation, Investigation, Writing – review & editing. JW: Writing – review & editing, Data curation. XL: Formal Analysis, Writing – review & editing, Conceptualization. XD: Conceptualization, Formal Analysis, Writing – review & editing. XQL: Investigation, Data curation, Writing – review & editing, Writing – original draft. DC: Investigation, Resources, Writing – review & editing, Conceptualization, Supervision, Funding acquisition, Data curation, Project administration, Writing – original draft, Validation.
Funding
The author(s) declare financial support was received for the research and/or publication of this article. The study was supported by the grant from the Finance Department of Jilin Province(JLSWSRCZX2020-0029, JLSWSRCZX2021-054) and the National Natural Science Foundation of China(82102110).
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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Summary
Keywords
breast, phyllodes tumor, osteosarcomatous differentiation, pathology, imaging
Citation
Guo L, Wang J, Li X, Dong X, Lu X and Cao D (2025) Case Report: Malignant phyllodes tumor of the breast with heterologous osteosarcomatous differentiation and literature review. Front. Oncol. 15:1635114. doi: 10.3389/fonc.2025.1635114
Received
26 May 2025
Accepted
08 September 2025
Published
23 September 2025
Volume
15 - 2025
Edited by
Shuhei Suzuki, Yamagata Prefectural Shinjo Hospital, Japan
Reviewed by
Edvin Ostapenko, OST Klinika, Lithuania
Natalia Camejo, Hospital of Clinics Dr. Manuel Quintela, Uruguay
Updates
Copyright
© 2025 Guo, Wang, Li, Dong, Lu and Cao.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Dianbo Cao, caodb@jlu.edu.cn
Disclaimer
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