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CORRECTION article

Front. Oncol., 04 September 2025

Sec. Molecular and Cellular Oncology

Volume 15 - 2025 | https://doi.org/10.3389/fonc.2025.1684497

Correction: Cucurbitacin B inhibits HIF-1α and attenuates non-small cell lung cancer via ZFP91

This article is a correction to:

  1. Cucurbitacin B inhibits HIF-1α and attenuates non-small cell lung cancer via ZFP91

    1. Read original article
Lei Song,&#x;Lei Song1,2†Jing Han&#x;Jing Han1†Run Wang&#x;Run Wang1†Shen CaoShen Cao1Yi TaiYi Tai1Xinyu WangXinyu Wang1Yanjin ZhengYanjin Zheng1Shufeng JinShufeng Jin1Yue XingYue Xing1Hong Xiang ZuoHong Xiang Zuo1Ming Yue LiMing Yue Li1Juan Ma*Juan Ma1*Xuejun Jin*Xuejun Jin1*
  • 1Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, Molecular Medicine Research Center, College of Pharmacy, Yanbian University, Yanji, Jilin, China
  • 2Affiliated Hospital of Yanbian University (Yanbian Hospital), Yanji, Jilin, China

A Correction on
Cucurbitacin B inhibits HIF-1α and attenuates non-small cell lung cancer via ZFP91

By Song L, Han J, Wang R, Cao S, Tai Y, Wang X, Zheng Y, Jin S, Shen Y, Liu CQ, Xing Y, Zuo HX, Li MY, Ma J and Jin X (2025) Front. Oncol. 15:1607264. doi: 10.3389/fonc.2025.1607264

Affiliation “Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, Molecular Medicine Research Center, College of Pharmacy, Yanbian University, Yanji, Jilin, China” was omitted for author Lei Song. This affiliation has now been added for author Lei Song.

Author Lei Song was erroneously assigned to affiliation “Department of Pharmacy, Yanbian University Hospital, Yanji, Jilin, China”. This affiliation has now been removed for author Lei Song.

There was a mistake in Figure 3E as published. The Western blot image for GAPDH was incorrectly uploaded. The corrected Figure 3 appears below.

Figure 3
Chemical structure of Cucurbitacin B (CuB) is shown in diagram A. Diagram B displays a bar graph of cell viability in different cancer cell lines (A549, HeLa, Hep3B, HCT116) treated with increasing CuB concentrations. Western blots and RT-PCR results in panels C-F demonstrate the expression of ZFP91 and GAPDH proteins in A549, HCT116, Hep3B, and HeLa cells at various CuB concentrations. Panel G presents immunofluorescence images showing ZFP91 localization and DAPI nuclear staining in cells treated with CuB at concentrations of 3, 10, and 30 micromolar, alongside a bar graph showing a decrease in relative green fluorescence with higher CuB concentrations.

Figure 3. CuB inhibits the protein and mRNA expression of ZFP91. (A) Chemical Formula of CuB. (B) The MTT assay was used to evaluate CuB’s effect on A549 cell viability. (C–F) Effect of CuB on protein and mRNA expression of ZFP91 in A549, HCT116, Hep3B, and HeLa Cells. (G) Effect of CuB on ZFP91 fluorescence intensity in A549 cells. Original magnification: 600×. Data are represented as mean ± standard, * p<0.05, ** p<0.01, *** p<0.001 compared with Control group.

There was a mistake in the caption of Figure 6B as published. The label for IP was incorrect and should read “IP: HA-HIF-1α”. The corrected caption of Figure 6 appears below.

Figure 6
Panels A and B show Western blot results examining interactions involving HIF-1α, ZFP91, and controls under various experimental conditions with CuB treatment. Panel C contains fluorescence microscopy images showing nuclear staining with DAPI and protein localization of ZFP91 and HIF-1α under different conditions including TNF-α and CuB treatments. Each row highlights different markers, with a final row displaying merged images.

Figure 6. CuB inhibited the interaction between ZFP91 and HIF-1α. (A, B) A549 cells were transfected with IP: HA-HIF-1α and Flag-ZNF91 plasmids for. Co-immunoprecipitation was then performed to pull down either Flag-ZNF91 or IP: HA-HIF-1α. (C) Co-localization of ZFP91 with HIF-1α within the cells was detected using immunofluorescence. Magnification: 600×.

Due to a clerical error by the author, “HIF-1α” was mistakenly written as “HID-1a” and should be corrected to “HIF-1α.”.

A correction has been made to the section Results, CuB inhibits the expression of HIF-1α through ZFP91:

“In addition, co-immunoprecipitation experiments demonstrated an interaction between ZFP91 and HIF-1α; however, the addition of CuB was found to attenuate this interaction (Figures 6A, B).”

The original version of this article has been updated.

Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Keywords: cucurbitacin B, non-small cell lung cancer, HIF-1α, ZFP91, anti-cancer

Citation: Song L, Han J, Wang R, Cao S, Tai Y, Wang X, Zheng Y, Jin S, Xing Y, Zuo HX, Li MY, Ma J and Jin X (2025) Correction: Cucurbitacin B inhibits HIF-1α and attenuates non-small cell lung cancer via ZFP91. Front. Oncol. 15:1684497. doi: 10.3389/fonc.2025.1684497

Received: 12 August 2025; Accepted: 27 August 2025;
Published: 04 September 2025.

Edited and reviewed by:

Luisa Lanfrancone, European Institute of Oncology (IEO), Italy

Copyright © 2025 Song, Han, Wang, Cao, Tai, Wang, Zheng, Jin, Xing, Zuo, Li, Ma and Jin. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Juan Ma, bWFqdWFuQHlidS5lZHUuY24=; Xuejun Jin, eGpqaW5AeWJ1LmVkdS5jbg==

†These authors have contributed equally to this work

Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.