ORIGINAL RESEARCH article
Front. Oral Health
Sec. Oral Infections and Microbes
Volume 6 - 2025 | doi: 10.3389/froh.2025.1615280
Construction of an HBPL antibacterial coating on a phase-transition lysozyme-modified titanium surface
Provisionally accepted
- 1School and Hospital of Stomatology，Tianjin Medical University., Tianjin, China
- 2Department of Stomatology, Tianjin Fifth Central Hospital, Tianjin, China
- 3Department of Pediatric Dentistry, School and Hospital of Stomatology, Tianjin Medical University, Tianjin, China
- 4Department of stomatology, Ecological City Hospital of Tianjin Fifth Central Hospital, Tianjin, China
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Background: In the field of dental implantation, titanium and its alloys serve as primary materials for implants due to their excellent biocompatibility. However, their insufficient antibacterial properties remain a critical limitation. Bacterial adhesion and subsequent biofilm formation on titanium alloy implant surfaces can trigger peri-implant inflammation, potentially leading to severe complications such as implant failure. To address this challenge, we developed a novel surface modification strategy that endows implants with dual functionality of antibacterial activity and enhanced cellular adhesion, thereby proposing a new approach for preventing and managing peri-implantitis.Methods: A layer-by-layer (LbL) self-assembly technique was employed to construct polyelectrolyte coatings composed of hyperbranched polylysine (HBPL) and hyaluronic acid (HA) on phasetransitioned lysozyme (PTL)-modified titanium surfaces. The surface characteristics were systematically investigated through scanning electron microscopy (SEM) and energy-dispersive Xray spectroscopy (EDS). Antibacterial efficacy was evaluated by monitoring bacterial viability and morphological alterations. Cytocompatibility assessments and molecular biological investigations were conducted to examine cellular responses and osteogenesis-related gene expression.Results: A novel polyelectrolyte coating with favorable biocompatibility and antibacterial properties was successfully fabricated on PTL-modified titanium surfaces. This coating demonstrated significant antimicrobial effects while concurrently promoting osteogenic differentiation to a certain extent.This study presents a dual-functional implant surface coating with combined antibacterial and osteogenic-enhancing capabilities. The developed strategy provides new insights for clinical surface modification of dental implants and offers a promising solution for peri-implantitis prevention and treatment.
Keywords: implant1, surface modified2, phase-transited lysozyme3, chitosan4, hyaluronic acid5, Hyperbranched poly-L-lysine6
Received: 21 Apr 2025; Accepted: 16 Jun 2025.
Copyright: © 2025 Li, Zhang, Yu, Zhang and Liang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Xiangyu Zhang, Department of Pediatric Dentistry, School and Hospital of Stomatology, Tianjin Medical University, Tianjin, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.