ORIGINAL RESEARCH article
Front. Pharmacol.
Sec. Ethnopharmacology
Volume 16 - 2025 | doi: 10.3389/fphar.2025.1549492
Oxytropis falcata Bunge extract Combined with Black soybean oil Ameliorates DNCB-induced Atopic dermatitis-like Skin Inflammation
Provisionally accepted
- 1The First Clinical Medical College, Gansu University of Chinese Medicine, Lanzhou, China
- 2Gansu Provincial Hospital, Lanzhou, Gansu Province, China
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Background: Oxytropis falcata Bunge (OF) is a traditional Tibetan medicine, while black soybean oil (BSO) is a contemporary treatment used for eczema. Pharmacological studies have indicated that both exhibit strong anti-inflammatory effects. However, the role of OF in atopic dermatitis remains uncertain. Objective: To investigate the anti-inflammatory effects and underlying mechanisms of Oxytropis falcata Bunge extracts (OFE) and BSO in DNCB-induced atopic dermatitis in mice. Methods: Mice were divided into six groups: positive control (1% mometasone furoate), OFE, BSO, OFE + BSO, DNCB, and control. After 20 days of local application of each ointment, therapeutic effects were evaluated. Histopathological examination was performed to assess skin thickness and mast cell infiltration. ELISA was used to quantify proinflammatory cytokines, real-time PCR measured IL-36 mRNA levels, and Western blotting analyzed HDAC3/NF-κB and CysLTR1 protein expression. Results: All treatments alleviated DNCB-induced atopic dermatitis symptoms, with the combination group showing the most significant improvement in epidermal thickness, mast cell infiltration, and dermatitis severity. In addition, the treatment groups suppressed activation of the HDAC3/NF-κB signaling pathway. Conclusions: The combination of OFE and BSO can effectively reduce DNCB-induced atopic dermatitis in mice. Its action does not rely on broad immunosuppression or induce skin toxicity and may involve inhibition of proinflammatory cytokine release and downregulation of the HDAC3/NF-κB signaling pathway.
Keywords: Oxytropis falcata Bunge, Black soybean, atopic dermatitis, HDAC3, NF-κB
Received: 24 Feb 2025; Accepted: 10 Jun 2025.
Copyright: © 2025 Chen, Wu, Wu, Bu, Liu, Zhao, Chen, Tian and Kai. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Yan Wu, The First Clinical Medical College, Gansu University of Chinese Medicine, Lanzhou, China
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