A comparative pharmacological study of three Chinese traditional medicines found Blautia to be the key functional bacterium of Coptis chinensis Franch. and Phellodendri Chinensis Cortex against colitis

Jiaguo ZhanJiale ChengYanan YangXiaofan XuZhiyi LuLeilei LiHaoyu LiQiannan YangYanran HuYuxin SongQingmiao FanEnwei YangQianyue LiangYing CaoFeng Qiu^*Shuangyong Sun^*Chongming Wu^*

• Tianjin University of Traditional Chinese Medicine, Tianjin, China

Background: Sanhuang refers to the three cold-natured and bitter-flavored traditional Chinese medicines namely Scutellaria baicalensis Georgi (HuangQin, HQ), Coptis chinensis Franch. (HuangLian, HL) and Phellodendri Chinensis Cortex (HuangBo, HB). Although similar in drug property, they are traditionally used to treat different dampness-heat syndromes belonging to upper-Jiao (lung and heart diseases), middle-Jiao (stomach and intestine diseases) and lower-Jiao (intestine, kidney and bladder diseases), respectively. The mechanisms behind their differential effects remain unexplored. Method: A model of large intestine dampness-heat syndrome colitis was established through the administration of exogenous hygrothermal conditions combined with lipopolysaccharide (LPS) and Escherichia coli. This model was employed to evaluate the efficacy of Phellodendri Chinensis Cortex, Coptis chinensisFranch., and Scutellaria baicalensis Georgi. Full-length 16S rRNA amplicon sequencing was utilized to assess changes in the gut microbiota following drug interventions. Ultimately, the therapeutic effects of key microbial strains on ulcerative colitis were confirmed using a dextran sulfate sodium (DSS)-induced colitis model.Results:The results showed that HL and HB exhibited significant remedial effects on LIDHS colitis, HQ did not. Gut microbial analysis revealed that HL and HB markedly shifted the overall structure of gut microbiota while HQ showed little impact. The increase of Blautia sp. was a common sign in both HL-and HB-treated animals, but it was not observed in HQ group. On the contrary, the abundance of a 4 Lactobacillus-dominant co-abundance gene group (CAG) was significantly declined in HL and HB groups but it was similar with the negative control in HQ group.Additionally, our observations indicate that the enrichment of Blautia is consistent with the difference in drug efficacy. In vivo experiments also demonstrated the anti-colitis efficacy of Blautia. Producta.Conclusion: This study identifies Blautia as the key bacterium against ulcerative colitis through the establishment of a novel model and drug comparison.