A novel drug that reduces pneumococcal toxicity by targeting pneumolysin (PLY): efficacy of the traditional Chinese medicine Radix Paeoniae Alba

Xu Yan¹Jing Han²Yonglin Zhou³Liping Sun^2*

• ¹Henan University of Chinese Medicine, Zhengzhou, Henan Province, China
• ²Changchun University of Chinese Medicine, Changchun, Jilin Province, China
• ³Ningxia University, Yinchuan, Ningxia Hui Region, China

Objective: While existing virulence-targeting strategies predominantly rely on single-component inhibitors that exert evolutionary pressure, this study pioneers an innovative approach using the multicomponent traditional Chinese medicine Radix Paeoniae Alba (RPA). Unlike conventional monotherapeutic agents, RPA uniquely inhibits pneumolysin (PLY) oligomerization without affecting bacterial growth, thereby circumventing resistance development—a critical limitation of current therapies. We aimed to elucidate the novel mechanism by which RPA attenuates Streptococcus pneumoniae pathogenicity by inhibiting the pore-forming activity of PLY while preserving host microbiota homeostasis according to the holistic TCM philosophy. Methods: Using a murine pneumococcal infection model, we evaluated the therapeutic effects of RPA on lung inflammation. Hemolysis assays and A549 cell viability tests were performed to assess PLY inhibition. Western blotting was used to characterize the PLY oligomerization dynamics following RPA treatment. Bacterial growth curves confirmed the nonantibacterial nature of RPA. Results: RPA significantly reduced pulmonary inflammation (p<0.05) and the levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α) without altering S. pneumoniae growth. Mechanistically, RPA inhibited