REVIEW article
Front. Pharmacol.
Sec. Pharmacology of Infectious Diseases
Volume 16 - 2025 | doi: 10.3389/fphar.2025.1615338
This article is part of the Research TopicPhytochemicals in Modern Medicine: Health Benefits and Toxicological ConsiderationsView all articles
Impact of Extraction Techniques on Phytochemical Composition and Bioactivity of Natural Product Mixtures
Provisionally accepted
Shicai Sun1
Ying Yu1Yunju Jo2
Jung Ho Han3Yingqi Xue2
Minkyoung Cho4Sung-Jin Bae5
Dongryeol Ryu2
Wonyoung Park6*
Ki-Tae Ha6*Shiwei Zhuang1*- 1Department of Surgery, Changchun University of Chinese Medicine, Changchun, Hebei Province, China
- 2Department of Biomedical Science and Engineering, School of Integrated Technology, Gwangju Institute of Science and Technology, Gwangju, Gwangju, Republic of Korea
- 3Korean Medicine Application Center, Korea Institute of Oriental Medicine, Daegu, North Gyeongsang, Republic of Korea
- 4Department of Parasitology and Tropical Medicine, and Institute of Health Sciences, Gyeongsang National University College of Medicine, Jinju, South Gyeongsang, Republic of Korea
- 5Department of Molecular Biology and Immunology, Kosin University College of Medicine, Busan, Republic of Korea
- 6School of Korean Medicine, Pusan National University, Busan, Busan, Republic of Korea
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Extraction methods critically influence the phytochemical profile and bioactivity of natural product mixtures, affecting their efficacy as therapeutic agents. This review assesses the impact of various extraction techniques-such as solvent-based extraction, ultrasound-assisted extraction, and enzymatic extraction-on the composition of bioactive compounds in plant extracts. We discuss how extraction parameters modify the bioactivity profiles, influencing their application in pharmaceutics and nutrition. This review critically analyzes these techniques with a special focus on integrated (hybrid) strategies, revealing that while advanced methods like ultrasound-assisted extraction and microwave-assisted extraction offer improved efficiency, the greatest potential lies in the synergistic combination of methods to maximize yield and preserve bioactivity. The insights gathered here aim to guide researchers in developing optimal and sustainable extraction protocols for therapeutic applications.
Keywords: Extraction techniques, Natural Products, bioactive compounds, Phytochemical composition, Pharmaceutical and Nutraceutical Applications
Received: 21 Apr 2025; Accepted: 21 Jul 2025.
Copyright: © 2025 Sun, Yu, Jo, Han, Xue, Cho, Bae, Ryu, Park, Ha and Zhuang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Wonyoung Park, School of Korean Medicine, Pusan National University, Busan, 46241, Busan, Republic of Korea
Ki-Tae Ha, School of Korean Medicine, Pusan National University, Busan, 46241, Busan, Republic of Korea
Shiwei Zhuang, Department of Surgery, Changchun University of Chinese Medicine, Changchun, Hebei Province, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.