ORIGINAL RESEARCH article
Front. Pharmacol.
Sec. Ethnopharmacology
Volume 16 - 2025 | doi: 10.3389/fphar.2025.1622178
This article is part of the Research TopicTreating Hepato-Intestinal Diseases with Herbal Medicines and their MetabolitesView all 6 articles
Sophora moocroftiana seeds ethanol extract regulates NLRP3 inflammasome activation and pyroptosis via ROS/TXNIP pathway to amelioration of NAFLD in vitro and in vivo
Provisionally accepted
RuiYing Yuan1MingAn Gui1
Shan Huang2YangZhen CiRen1DongZhi ZuoMa1GuiBin Yang1WenDong Zhang1
Sicen Wang1,3*
Bin Li1,2*- 1Tibet University, Lhasa, China
- 2Qingdao University of Science and Technology, qingdao, China
- 3Xi'an Jiaotong University, Xi'an, China
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Background: Sophora moorcroftiana (Benth.) Baker is a perennial shrub endemic to the Tibetan Plateau. Its seeds are traditional Tibetan medicine for treating jaundice, hepatitis, purulent tonsillitis, diphtheria, and parasitosis. Aim of the study: The aim of this research is to explore the possible processes by which the 70% ethanol extract of S. moocroftiana seeds (SMS) imparts healing effects in NAFLD what is done by simulating the disease state of NAFLD via the creation of a lipid accumulation model in HepG2 cells induced by FFA, and a NAFLD model in C57BL/6J mice induced by HFD. Materials and methods: The experiment used HFD fed C57BL/6J mice to establish a NAFLD model, which was divided into a control group, HFD model group, different doses of SMS groups, and a Silymarin group. were used in the experiment. SMS could improve lipidGSDMD Gasdermin D GSH-PX Glutathione peroxidase H&E staining Hematoxylin eosin staining HDL-C High-density lipoprotein cholesterol HPLC High performance liquid chromatography IgG Immunoglobulin G IL-18 Interleukin-18 IL-1β Interleukin-1β LDH Lactate dehydrogenase LDL-C Low-density lipoprotein cholesterol MDA Malondialdehyde MEM Minimum essential medium MTT 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide NAFLD Non-Alcoholic Fatty Liver Diseas NLRP3 NOD-like receptor thermal protein domain associated protein 3 OD Optical density P/S Penicillin/streptomycin PMSF Phenylmethanesulfonyl fluoride qRT-PCR Quantitative real-time polymerase chain reaction RIPA Radioimmunoprecipitation lysis buffer ROS Reactive oxygen species SD Standard deviation SDS-PAGE Sodium dodecyl sulfate-polyacrylamide gel electrophoresis SEM Scanning electron microscopy siRNA Small interfering RNA SMS 70% ethanol extract of S. moocroftiana seeds SOD Superoxide dismutase TC Total cholesterol TG Triglyceride TNF-α Tumor necrosis factor-α TRX Thioredoxin TXNIP Thioredoxin-interacting protein WB Western blotting
Keywords: Sophora moocroftiana seeds, Non-alcoholic fatty liver disease, pyroptosis, NLRP3 inflammasome, ROS/TXNIP pathway
Received: 02 May 2025; Accepted: 05 Aug 2025.
Copyright: © 2025 Yuan, Gui, Huang, CiRen, ZuoMa, Yang, Zhang, Wang and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Sicen Wang, Tibet University, Lhasa, China
Bin Li, Tibet University, Lhasa, China
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