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ORIGINAL RESEARCH article

Front. Pharmacol.

Sec. Ethnopharmacology

Volume 16 - 2025 | doi: 10.3389/fphar.2025.1691629

Development and Validation of a UHPLC-QqQ-MS/MS Method for Simultaneous Determination of Fifteen Major Components from Oroxyli Seed Extract in Rat Plasma and Application to the Pharmacokinetic Study

Provisionally accepted
Zhenguo  LvZhenguo LvJinyue  MaJinyue MaKaili  ZhangKaili ZhangWenwen  LiWenwen LiZijing  ZhangZijing ZhangLu  ChenLu ChenWenhan  LinWenhan LinYameng  ZhuYameng ZhuMinglei  GeMinglei GeHuizi  OuyangHuizi OuyangJun  HeJun He*
  • Tianjin University of Traditional Chinese Medicine, Tianjin, China

The final, formatted version of the article will be published soon.

For this investigation, a UHPLC-QqQ-MS/MS method was developed for the simultaneous determination of fifteen components (chrysin, chrysin-7-O-β-D-glucuronide, hispidulin, wogonoside, quercetin, quercetin-7-O-β-D-glucoside, baicalin, baicalein, acacetin, oroxin A, oroxin B, oroxylin A, oroxylin A-7-O-β-D-glucuronide, apigenin, and scutellarein) in rat plasma. An ACQUITY UPLC BEH C18 column was employed for separation, and simultaneous detection of analytes was achieved through multiple reaction monitoring mode. Method validation results were all within acceptable ranges for biological sample determination. Subsequently, the method was applied to pharmacokinetic studies in rats following oral administration of Oroxyli Seed (OS) extract. Results indicated that baicalin, oroxylin A, and oroxylin A-7-O-β-D-glucuronide exhibited significant bimodal phenomena. The maximum concentration of baicalin was 13376.96 ± 2232.32 ng/mL, indicating a relatively high blood concentration. This may be related to the high content of oroxin A and oroxin B in OS extract being metabolized to baicalin. The present study may provide guidance for the further application of OS.

Keywords: Oroxyli Seed extract, Oroxylum indicum (L.) Vent., pharmacokinetics, UHPLC-QqQ-MS/MS, Rat plasma

Received: 24 Aug 2025; Accepted: 30 Sep 2025.

Copyright: © 2025 Lv, Ma, Zhang, Li, Zhang, Chen, Lin, Zhu, Ge, Ouyang and He. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Jun He, hejun673@tjutcm.edu.cn

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