ORIGINAL RESEARCH article
Front. Pharmacol.
Sec. Ethnopharmacology
Identification of active metabolites in alcohol-water of Gentiana scabra Bunge extract to promotes chicken fracture healing
Provisionally accepted
- 1Wenzhou Academy of Agricultural Sciences, Wenzhou, China
- 2China Jiliang University, Hangzhou, China
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Fractures are common clinical conditions. Gentiana scabra bunge extract ( GSE ) exhibits multiple biological activities, including anti-nociceptive and anti-inflammatory effects. However, its therapeutic effects and active metabolites regarding fracture healing remain poorly understood. In this study, we aimed to investigate the role and active metabolites of GSE in fracture healing by using chicken fracture model. The experiments utilized column chromatography, X-ray imaging, high-performance liquid chromatography ( HPLC ), and liquid chromatography-mass spectrometry ( LC-MS ). The results demonstrated that GSE mother liquor and its fractions GSE30, GSE50, GSE70, GSE90 promoted fracture healing. Compared to the control group, the callus area in the GSE group was increased by 1.8-fold on day 3 and by 2.0-fold on day 10. The most favorable healing effect was observed in the GSE50 group, this fraction was primarily composed of gentisin ( 27.6% ), gentiopicroside ( 7.3% ), and isoorientin ( 65.1% ). A notable dose-response relationship was observed between gentisin and isoorientin concentrations in GSE and bone repair outcomes.
Keywords: Fracture Healing, Gentiana scabra Bunge, column chromatography, Fraction, dose-response
Received: 17 Sep 2025; Accepted: 30 Oct 2025.
Copyright: © 2025 Liu, Wang, Jiang, Luo, Liu and Luo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Shuai Luo, luoshuai@wzvcst.edu.cn
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