ORIGINAL RESEARCH article
Front. Pharmacol.
Sec. Translational Pharmacology
DICAR/DICAR-JP exerts therapeutic effects in brain stroke via the miR-361-5p/PRMT1 pathway
Provisionally accepted
- 1Wuhan University of Science and Technology, Wuhan, China
- 2Southwest Medical University, Luzhou, China
- 3Zhongshan Hospital Fudan University, Shanghai, China
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Background: Angiogenesis is an important mechanism in stroke therapy. Circular RNA-DICAR is known to protect against diabetes-induced cardiomyocyte pyroptosis. In this study, we examined the effect of DICAR on angiogenesis as well as the therapeutic effect of DICAR-JP, which is its functional domain, against stroke. The mechanism involves the miR-361-5p/PRMT1 signaling pathway. Moreover, the middle cerebral artery occlusion/reperfusion (MCAO) method was used to establish a mouse stroke model in the DICAR-Tg mouse model. Methods: AAV9-DICAR-JP was constructed and injected into the cerebral ventricles. Furthermore, tube formation assays were used to evaluate the in vitro activity of DICAR-JP. The blood flow was tested by Laser Speckle Flowgraphy. The expressions of PRMT1 protein expression was evaluated by Western Blotting (WB). The binding ability was evaluated by luciferase reporter. Results: Results indicated that DICAR-Tg improved neuronal function. AAV9-DICAR-JP increased blood flow in the brain following stroke. DICAR-siRNA upregulated miR-361-5p expression, and overexpression of miR-361-5p significantly reduced PRMT1 expression. Conclusion: DICAR/DICAR-JP has potential therapeutic benefits in stroke, which are mediated by the miR-361-5p/PRMT1 signaling pathway.
Keywords: Stroke, diabetes-induced circulation-associated circular RNA, functional domain, Angiogenesis, miR-361-5p, Prmt1
Received: 09 Oct 2025; Accepted: 30 Oct 2025.
Copyright: © 2025 Yu, Jiang, Yang, Zhan, Xiang, Chen, Kuai and Yuan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Zheng Kuai, kuai.zheng@zs-hospital.sh.cn
Qiong Yuan, yuanqiongwh@163.com
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