ORIGINAL RESEARCH article
Front. Vet. Sci.
Sec. Veterinary Infectious Diseases
Volume 12 - 2025 | doi: 10.3389/fvets.2025.1542992
Isolation and Identification of Mycoplasma hyorhinis and Virulence Evaluation of Its Field Isolates
Provisionally accepted
- Huazhong Agricultural University, Wuhan, China
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As a prevalent bacterial swine pathogen worldwide, Mycoplasma hyorhinis (M. hyorhinis, Mhr) is associated with various diseases, including multiple serositis, pneumonia, arthritis, and otitis media. It is also linked to the porcine respiratory disease complex (PRDC). Currently, research on the attributes and pathogenic mechanisms of Mhr remains limited. This study aimed to determine the positivity rate of Mhr in clinical lung samples from China in 2022, isolate and identify field strains, and investigate the pathogenicity of different Mhr isolates through piglet infection trials. The results revealed a 31.77% positivity rate of Mhr in clinical lung samples. Three Mhr isolates (ZZ-1, GD-1, and AH-1) were successfully purified, and multilocus sequence typing (MLST) identified their sequence types as ST166, ST167, and ST144, respectively.Animal infection trials demonstrated that Mhr could induce polyserositis, pneumonia, and arthritis in piglets. Moreover, variations in virulence were observed among different Mhr strains. These findings contribute to identifying and assessing the threats posed by different strains to pig health, guiding the development of clinical prevention and control strategies, and promoting in-depth research into the pathogenic mechanisms of Mhr.
Keywords: Mycoplasma hyorhinis, Positivity rate, Isolation, IDENTIFICATION, Infection
Received: 10 Dec 2024; Accepted: 25 Apr 2025.
Copyright: © 2025 Yang, Yang, Duan, Qian, Ma, Jia, Huo, Dong, Chen and Tan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Chen Tan, Huazhong Agricultural University, Wuhan, China
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