ORIGINAL RESEARCH article
Front. Vet. Sci.
Sec. Animal Reproduction - Theriogenology
This article is part of the Research TopicInnovative Strategies for Improving Sperm Lifespan during StorageView all 6 articles
Cryoprotectant Effects of Mangiferin on the Quality of Frozen-Thawed Boar Sperm
Provisionally accepted
- 1Wuxi Xishan People's Hospital, Wuxi, China
- 2Yanbian University Agriculture College, Longjing, China
- 3Zhongda Hospital, Southeast University, Nanjing, China
- 4Southeast University Zhongda Hospital Department of Urology, Nanjing, China
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Abstract: Boar sperm is particularly vulnerable to damage at low temperatures, leading to reduced efficiency in cryopreservation. This study aimed to analyze the effect of mangiferin (Man) on boar sperm cryopreservation efficiency. Various concentrations of Man (0, 5, 10, 20, and 40 µM) were added into the cryopreservation extender and analyzed for their effects on sperm motility, kinetic parameters, acrosome integrity, plasma membrane integrity, DNA integrity, mitochondrial membrane potential, total antioxidant capacity (T-AOC) levels, hydrogen peroxide (H2O2) content, glutathione (GSH) levels, malondialdehyde (MDA) levels, and reactive oxygen species (ROS) levels. The results indicate that, in comparison to the control group, 10 µM Man significantly enhances sperm total motility, progressive motility, average path velocity, average straight-line velocity, average curvilinear velocity, and beat frequency during the cryopreservation of boar semen. Furthermore, our studies demonstrated that 10 µM Man significantly enhance mitochondrial membrane potential, acrosome integrity, plasma membrane integrity, and DNA integrity in boar sperm after thawing. Additionally, treatment with 10 µM Man markedly increase GSH levels and T-AOC in boar sperm, while simultaneously reducing MDA, H2O2, and ROS levels. Consequently, the findings of this study suggest that treatment with 10 µM Man substantially improve the efficiency of boar semen cryopreservation.
Keywords: antioxidant, boar sperm cryopreservation, Mangiferin, ROS, sperm quality
Received: 15 Sep 2025; Accepted: 16 Dec 2025.
Copyright: © 2025 Minhao, Luo, Mao, Zhang and Gu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Weipu Mao
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