BRIEF RESEARCH REPORT article
Front. Vet. Sci.
Sec. Veterinary Clinical, Anatomical, and Comparative Pathology
Establishment of a TaqMan-based quantitative real-time PCR for the detection of Porcine Parvovirus
Zhiqiang Hu 1
Maosi Xu 2
Guoqiang Tang 3
Ran Guan 1
Xingsheng Lai 2
Kelei Zhou 4
Hao Li 1
Yadong Jin 1
Jingang Zhao 1
Wei Xu 5
Zengwen Huang 1
1. Xichang University, Xichang, China
2. Shandong Zhongxin Food Group Co., Ltd., Jinan, China
3. Bureau of Agriculture and Rural Af- 9 fairs of Liangshan Yi Autonomous Prefecture, Xichang City, China
4. Bureau of Agriculture and Rural Af- 11 fairs of Xichang City, Xichang City, China
5. Zhejiang University, Hangzhou, China
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Abstract
Porcine Parvovirus (PPV) is a non-enveloped DNA virus that predominantly induces reproductive disorders in swine. The ongoing emergence of novel PPV variants and the frequent co-infections with other viruses have led to significant economic losses within the swine industry. This study, utilizing 31 previously reported complete PPV genome sequences, identified a conserved fragment of the PPV-NS1 gene through homology analysis. A TaqMan-based real-time quantitative PCR (TaqMan-qPCR) detection method was developed targeting this specific fragment. Sensitivity assessments determined a detection limit of 8.5 copies/μL for standard plasmids. Specificity assessments showed no cross-reactivity with ten other prevalent swine pathogens. The coefficients of variation for both intra-assay and inter-assay repeatability tests were both under 1%, demonstrating high reproducibility. Moreover, an analysis involving 32 clinical samples was conducted to compare the detection outcomes of the developed method with those obtained from a commercial kit. The findings demonstrated that the established method achieved a relative sensitivity of 100% and a relative compliance rate of 75%, suggesting its potential as an alternative to the commercial kit. In summary, the TaqMan-qPCR method developed in this study exhibits high sensitivity and specificity, making it ideal for detecting various clinical samples. It also provides a valuable tool for monitoring PPV and examining its epidemiological traits.
Summary
Keywords
Clinical samples, NS1 gene, Porcine parvovirus, Sensitivity, TaqMan-qPCR
Received
31 October 2025
Accepted
17 February 2026
Copyright
© 2026 Hu, Xu, Tang, Guan, Lai, Zhou, Li, Jin, Zhao, Xu and Huang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Zengwen Huang
Disclaimer
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.