ORIGINAL RESEARCH article
Front. Vet. Sci.
Sec. Veterinary Infectious Diseases
This article is part of the Research TopicMolecular Host-Pathogen Interactions in Veterinary Infectious Diseases: From Pathogenesis to Immunomodulatory TherapiesView all 5 articles
Dysregulated interferon signaling and hyperinflammation upon FCGBP knockdown in goat bronchial epithelial cells infected with Pasteurella multocida
Provisionally accepted
- Hainan University, Haikou, China
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The Fc gamma binding protein (FCGBP) is pivotal for mucosal immune defense against Pasteurella multocida (P. multocida), a key pathogen that induces respiratory ailments in ruminants. However, the mechanism by which FCGBP modulates the pulmonary immune response following infection remains unclear. This study investigated the role of FCGBP in the immune response of goat bronchial epithelial cells against P. multocida infection. An in vitro infection model was established using FCGBP-knockdown goat bronchial epithelial cells infected with P. multocida serotype D, and transcriptome sequencing coupled with bioinformatics was then used to detect differentially expressed genes (DEGs) and elucidate their regulatory networks. The expression levels of key DEGs and inflammatory factors were subsequently validated via quantitative real-time PCR (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA). The results indicated that FCGBP maintains homeostasis and provides immune protection in goat lungs. Knockdown of FCGBP in goat bronchial epithelial cells leads to impaired cellular barrier function, resulting in excessive activation of type I interferon, HIF-1, TNF, and NOD-like receptor signaling pathways following infection with P. multocida. These findings offer a foundational framework for elucidating the immune function of FCGBP in goat bronchial epithelial cells infected with P. multocida.
Keywords: Fcgbp, goat bronchial epithelial cells, Inflammation, Pasteurella multocida, RNA-Seq
Received: 06 Dec 2025; Accepted: 16 Feb 2026.
Copyright: © 2026 Yang, Chen, Wang, Wu, Du, Gao, Chen, Chen and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Qiaoling Chen
Fengyang Wang
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