In the original article, there was a mistake in Figure 3 as published. C&E “p-rpS6(S240/242)” should be “p-rpS6(S240/244),” G “bar-headed line symbol after Bax/Bcl2” should be “arrow symbol.”
Figure 3
Additionally, there was a mistake in the legend for Figure 6 as published. H “PD22 mice” should be “PD28 mice”. The corrected legend appears below.
Figure 6 ∣ The aromatase-promoting effects of VCD. (A) Immunoblotting with antibodies against aromatase and β-actin of extracts from primary ovarian GCs of PD20 mice treated with VCD (1 nM, 10 nM, 100 nM, or 1 μM) or FSH (50 ng/ml) for 24 h. (B) Quantitation of (A) using the gray value detection module of ImageJ; ** indicates p < 0.01 compared to untreated controls by Student's t-test. The data are presented as the mean ± S.D. of n = 3 biological replicates per group. (C,D) The β-actin-normalized protein expression levels of aromatase in cultured PD12 ovaries exposed to 10 nM VCD for 24 h were analyzed using immunoblotting, with quantification using the ImageJ software. Representative blot images are shown. ** indicates p < 0.01 compared to the drug-paired controls by Student's t-test. The data are presented as mean ± S.D. of n = 3 biological replicates per group. (E,F) The human granulosa-like tumor KGN cell line cultured in FBS-free DMEM/F12 medium exposed to VCD at 10 nM, 100 nM, 1 μM, and 10 μM or FSH (50 ng/ml) for 24 h was analyzed using immunoblotting, with quantification using the ImageJ software. Representative blot images are shown. ** indicates p < 0.01 compared to the drug-paired controls by Student's t-test. The data are presented as the mean ± S.D. of n = 3 biological replicates per group. (G) Histological examination of ovarian aromatase-stained PD12 mice exposed to VCD at 10 nM or 10 μM for 24 h in vitro. Scale bar = 100 μm. (H) Histological examination of ovarian aromatase-stained PD28 mice IP injected with VCD (80 mg/kg) once each day for 5 days. Scale bar = 100 μm. (I) Exposure to VCD at 10 pM, 100 pM, 1 nM, 10 nM, 100 nM, 1 μM, 10 μM, or 100 μM (or PBS control) for 24 h. The estrogen levels in the KGN cell culture supernatants were measured at 24 h using ELISA. The data are presented as the mean ± S.D. of n = 3 biological replicates per group. (J) VCD exposure for 24 h (with or without the aromatase substrate testosterone at 10 nM), followed by ELISA-based measurement of estrogen levels at 24 h in the PD12 ovarian culture supernatants. PBS was the negative control and FSH (50 ng/ml) was the positive control, letrozole (Let) is used as aromatase inhibitor. The data are presented as the mean ± S.D. of n = 3 biological replicates per group.
In the section of “MATERIALS AND METHODS,” **Paragraph 1** of subsection titled **Ovary Transplantation Under the Kidney Capsule**, “The paired ovaries (VCD-treated and paired controls) from the same donor were excised and separately allografted into two 2-month-old host mice followed by IP injection of VCD at 80 mg/ml for three consecutive days.” Should be “The paired ovaries (VCD-treated and paired controls) from the same donor were excised and separately allografted into two 2-month old host mice followed by IP injection of VCD at 80 mg/kg for three consecutive days.”
In the section of “RESULTS,” **Paragraph 2** of subsection titled **Very Short-Term VCD Exposure Promotes the Activation of Primordial Follicles by Transiently Activating the PI3K/Akt/mTOR Pathway, Whereas Extended Exposure Prevents Further Follicular Activation**, “Specifically, allograft-paired ovaries from a single PD2 donor mouse were separately transplanted into two 2-month-old ovariectomized host mice, one of which was exposed to VCD (via IP injection, 80 mg/ml daily for 3 days).” Should be “Specifically, allograft-paired ovaries from a single PD2 donor mouse were separately transplanted into two 2-month-old ovariectomized host mice, one of which was exposed to VCD (via IP injection, 80 mg/kg daily for 3 days).
A correction has been made to **Ovary Transplantation Under the Kidney Capsule**, **Paragraph 1** and **Very Short-Term VCD Exposure Promotes the Activation of Primordial Follicles by Transiently Activating the PI3K/Akt/mTOR Pathway, Whereas Extended Exposure Prevents Further Follicular Activation**,**Paragraph 2**
Corrected paragraph:
“The kidneys of the anesthetized host animals were externalized through a dorso- longitudinal incision. The paired ovaries (VCD-treated and paired controls) from the same donor were excised and separately allografted into two 2-monthold host mice followed by IP injection of VCD at 80 mg/kg for three consecutive days. The age-matched paired control groups were treated with an equal amount of 0.9% normal saline. Starting at 3 days after ovary transplantation donated by PD12 mice, the hosts were administrated 2 IU of follicle-stimulating hormone (FSH) per mouse via daily IP injection, and the grafts were harvested 7 or 14 days later for histological examination. No FSH treatment was given to the host mice that received ovarian grafts from PD2 donors.”
“Pursuing the supposition that VCD might protect primordial and primary follicles from atretic degeneration, we next conducted experiments following Li et al. (2010) based on ovarian grafts from PD2 mice onto the kidney of a 2-month-old mouse. Specifically, allograft-paired ovaries from a single PD2 donor mouse were separately transplanted into two 2-month-old ovariectomized host mice, one of which was exposed to VCD (via IP injection, 80 mg/kg daily for 3 days). Upon examination at post ovarian graft day 7, the transplanted ovaries of the VCD-exposed mice appeared larger and had significantly greater numbers of secondary follicles than the transplanted ovaries of the untreated control host mice (the mean number of secondary follicles was more than 5.2-fold higher in VCD-exposed mice; P < 0.05). No differences were observed in the numbers of primordial or primary follicles (Figures 2C–F).
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
References
1
LiJ.KawamuraK.ChengY.LiuS.KleinC.LiuS.et al. (2010). Activation of dormant ovarian follicles to generate mature eggs. Proc. Natl. Acad. Sci. U.S.A.107, 10280–10284. 10.1073/pnas.1001198107
Summary
Keywords
VCD, ovotoxicity, folliculogenesis, ovulation induction, PI3K-Akt pathway
Citation
Cao LB, Liu HB, Lu G, Lv Y, Leung CK, Du YZ, Wang WM, Xiong ZQ, Su XW, Li HJ, Chen Z-J, Ma JL and Chan WY (2020) Corrigendum: Hormone-Like Effects of 4-Vinylcyclohexene Diepoxide on Follicular Development. Front. Cell Dev. Biol. 8:607067. doi: 10.3389/fcell.2020.607067
Received
16 September 2020
Accepted
17 September 2020
Published
03 December 2020
Approved by
Frontiers Editorial Office, Frontiers Media SA, Switzerland
Volume
8 - 2020
Updates
Copyright
© 2020 Cao, Liu, Lu, Lv, Leung, Du, Wang, Xiong, Su, Li, Chen, Ma and Chan.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Gang Lu lugang@cuhk.edu.hkJin Long Ma majinlong123@hotmail.comWai Yee Chan chanwy@cuhk.edu.hk
This article was submitted to Cell Death and Survival, a section of the journal Frontiers in Cell and Developmental Biology
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