Abstract
Mitophagy is a specialized autophagic pathway responsible for the selective removal of damaged or dysfunctional mitochondria by targeting them to the autophagosome in order to maintain mitochondria quality. The role of mitophagy in tumorigenesis has been conflicting, with the process both supporting tumor cell survival and promoting cell death. Cancer cells may utilize the mitophagy pathway to augment their metabolic requirements and resistance to cell death, thereby leading to increased cell proliferation and invasiveness. This review highlights major regulatory pathways of mitophagy involved in cancer. In particular, we summarize recent progress regarding how nuclear-encoded long non-coding RNAs (lncRNAs) function as novel epigenetic players in the mitochondria of cancer cells, affecting the malignant behavior of tumors by regulating mitophagy. Finally, we discuss the potential application of regulating mitophagy as a new target for cancer therapy.
Introduction
Mitochondria play a central role in cellular bioenergetics, regulating essential biochemical reactions that generate adenosine triphosphate (ATP) and reactive oxygen species (ROS; Friedman and Nunnari, 2014; Spinelli and Haigis, 2018; Missiroli et al., 2020). Abnormalities in respiratory chain structure or mutations of mitochondrial genome DNA can interfere with normal mitochondrial functions, leading to an imbalance in calcium ion homeostasis, ROS production, and apoptosis. Mitochondria quality control (MQC) is a critical mechanism to maintain mitochondrial health and mitochondrial homeostasis (Pickles et al., 2018), including mitochondria biogenesis, fission and fusion, and mitophagy (Wai and Langer, 2016). Mitophagy, a specialized form of autophagy that degrades dysfunctional mitochondria, serves as the primary mechanism to regulate mitochondrial functions, including energy metabolism, metabolic reprogramming, and mitochondria self-repair and renewal (Pickles et al., 2018; Roca-Portoles and Tait, 2021). Mitophagy is activated by hypoxia, metabolic stress, and mitochondrial depolarization through regulatory pathways involving PINK1/Parkin, BNIP3/NIX, and FUNDC1 (Poole and Macleod, 2021).
The concept of mitochondrial degradation was first proposed by Margaret and Warren Lewis as early as 1915 (Lewis and Lewis, 1915). Later studies using electron microscopy demonstrated the presence of mitochondria within lysosomes (Clark, 1957). However, this morphological observation was not followed up with functional experiments until the landmark studies of Okamoto et al. (2009), who identified ATG32 as a critical receptor of mitophagy in yeast, a finding which won the Nobel Prize in 2016. Since then, studies on mitophagy have dramatically increased (Figure 1; Montava-Garriga and Ganley, 2020).
FIGURE 1
The role of mitophagy in tumorigenesis has recently been studied in detail. Since mitophagy leads to the degradation of dysfunctional mitochondria and decreased ROS production, mitophagy was initially thought to be related to tumor suppression. However, it has been shown that mitophagy actually plays a complicated role in tumor growth. Mitophagy is vital for metabolic remodeling within tumor cells and for modulating interactions among tumor cells, but its role is complex and depends on the type and stage of the tumor.
Recent studies have shown that lncRNAs, a class of long non-coding RNA (lncRNA) molecules with a length of >200 bp, have emerged as critical regulators in cancer. Many lncRNAs are transcribed from the human genome, and a number of them are involved in the regulation of mitophagy and mitophagy-associated drug resistance. LncRNAs can regulate various cellular functions, including mitochondrial metabolic reprogramming, to meet the needs of tumor metabolism. Therefore, it is important to understand the relationship between lncRNAs and mitophagy regulation in cancer. Exploiting the newly emerging knowledge of the lncRNA-mitophagy-cancer axis may provide novel targets for cancer therapy. In this review, we will summarize recent progress on the role of lncRNAs in malignancy, demonstrating the importance of mitochondria-associated lncRNAs in cancer metabolism, apoptosis, and mitophagy (Dong et al., 2017; Zhao et al., 2018; Anand and Pandi, 2021).
The Role of Mitophagy in Cancer
Regulatory Pathways of Mitophagy
The main physiological function of mitophagy is to ensure the recycling of old and damaged organelles through continuous mitochondrial housekeeping (Palikaras et al., 2018). Little is known about steady-state mitophagy levels (also known as basal mitophagy) as mitophagy is typically examined under stress-induced conditions. Cells undergo basal mitophagy as part of routine mitochondrial maintenance (McWilliams et al., 2016). In tissues that consume large amounts of ATP, such as the brain, skeletal muscle, heart, liver and kidney, mitochondrial biogenesis is actively maintained to meet the cells’ metabolic requirements. When cells are switched from an energy-consuming state to a stable state, they tend to activate mitophagy in order to reduce mitochondrial mass as an adaptation to changes in cell metabolism. Proper homeostasis of mitochondrial mass is therefore critical to maintaining biological resilience (Onishi and Okamoto, 2021). Mitophagy is also induced during cell fate determination, such as erythrocyte differentiation (Schweers et al., 2007; Sandoval et al., 2008), cardiomyocyte maturation (Gong et al., 2015; Gottlieb and Bernstein, 2015; Kuznetsov et al., 2020; Morales et al., 2020), the developmental transitions of muscle tissue (Sin et al., 2016), and stem cell pluripotency (Vazquez-Martin et al., 2016; Xiang et al., 2017). In addition, mitophagy regulates the elimination of sperm mitochondria, thereby avoiding the inheritance of paternal mitochondrial DNA (mtDNA; Rojansky et al., 2016; Song et al., 2016). Since mitophagy plays a crucial role in maintaining mitochondrial homeostasis (Bin-Umer et al., 2014), it is not surprising that defective, inadequate, or excessive mitophagy can result in pathological conditions (Palikaras et al., 2017), such as neurodegenerative disease (Um and Yun, 2017; Tran and Reddy, 2020; Wang et al., 2021), cardiovascular disease (Billia et al., 2011; Zhang W. et al., 2016; Zhang et al., 2017; Chang et al., 2020), metabolic disorders (He et al., 2021; Pang et al., 2021; Zia et al., 2021), inflammation (Sliter et al., 2018; Lee et al., 2020), liver disease (Ke, 2020; Kouroumalis et al., 2021), aging (Richard et al., 2013; Cornelissen et al., 2018), and cancer.
Mitophagy is induced during stress to mediate metabolic adjustments to an external challenge. Mitophagy is highly regulated by various signaling pathways that can be roughly classified as ubiquitin (Ub)-dependent and Ub-independent mitophagy. The PINK1/Parkin pathway relies on a Ub-dependent mitophagy mechanism (Greene et al., 2012; Yamano and Youle, 2013; Harper et al., 2018). After PINK1-mediated phosphorylation, the E3 ligase PARKIN is translocated to mitochondria, where it induces the ubiquitination of the outer membrane of mitochondria. The mitophagy cargo adaptors p62, OPTN, and NDP52 bind to the phospho-Ub chains and interact with processed LC3 to target mitochondria for phagosome degradation (Figure 2A; Trempe et al., 2013; Kazlauskaite et al., 2014; Koyano et al., 2014). The inner mitochondrial membrane (IMM) protein PHB2 is a crucial mitophagy receptor that is required for Parkin-induced mitophagy (Wei et al., 2017; De Falco et al., 2020). BNIP3, BNIP3-like (BNIP3L) or NIX proteins and FUNDC1, on the other hand, function via Ub-independent mitophagy (Schweers et al., 2007; Liu et al., 2012; Wu et al., 2014). After hypoxia or other stress stimulation, BNIP3 and NIX can form stable homodimers in the outer mitochondrial membrane and recruit LC3 to induce mitophagy (Figure 2B; Rogov et al., 2017; Marinković et al., 2021; Springer et al., 2021). Both pathways cooperate to ensure efficient mitophagy (Lee et al., 2011; Zhang T. et al., 2016).
FIGURE 2
Mitophagy as a Positive Promoter of Tumorigenesis
The PINK1/Parkin pathway activates mitophagy in a Ub-dependent manner in cancer. As compared with non-transgenic controls, Parkin-knockout (KO) mice exhibited a significantly lower tumor burden of melanoma, including tumor size and lung metastases (Lee Y. S. et al., 2018). PINK1 was upregulated in esophageal squamous cell carcinoma (ESCC) tissues from patients who received chemotherapy (Yamashita et al., 2017). ESCC patients with high CD44 expression were more likely to have distant metastases and poor prognosis. Interestingly, mitophagy is increased in CD44 high-expressing cells (Whelan et al., 2017). Depletion of Parkin or inhibition of autophagy with chloroquine reduced CD44 high-expressing cells both in vitro and in vivo. Collectively, these studies suggest that PINK1/Parkin-dependent mitophagy may play an essential role in promoting the development of tumors.
BNIP3 plays a pro-tumorigenic role by driving aggressive features in melanoma cells, including migration, clonal growth, cell survival, and vasculogenic mimicry (Maes et al., 2014). Knockdown of baseline BNIP3 in melanoma cells caused accumulation of actin stress fibers and membrane ruffles and induced aberrations in cytoskeletal structures. Thus, BNIP3 maintains the plasticity of the actin cytoskeleton, accounting for cell migration and cancer progression. By analyzing immunohistochemical scores, Jiang et al. (2018) found that elevated expression of BNIP3 was found in 31.9% (15/47) of patients with uveal melanoma. High expression of BNIP3 was associated with deeper scleral invasion and lower cancer survival. These studies suggest that BNIP3/NIX-dependent mitophagy may play a pro-tumorigenic role in the development of cancer.
FUNDC1 also plays a critical role in hypoxia-induced mitophagy. Hui et al. (2019) found that FUNDC1 was upregulated in tumor tissues of laryngeal cancer patients in parallel with lipid peroxidation. Treatment of laryngeal cancer cells with low doses of hydrogen peroxide upregulated FUNDC1 through the ERK1/2 signal pathway and promoted proliferation of laryngeal cancer cells. Similarly, FUNDC1 was also upregulated in cervical cancer tissues, and the FUNDC1 expression score was an independent factor to determine overall and disease-free survival. Knockdown of FUNDC1 reduced the proliferation of cervical cancer cells and enhanced cell sensitivity to cisplatin and radiotherapy (Hou et al., 2017).
Recent studies have shown that mitophagy plays an important role in the metabolic transition of tumors and the maintenance of phenotype of cancer stem cells (CSCs). Continuous reprogramming of cellular metabolism is a critical milestone event in cancer (Pavlova and Thompson, 2016; Chen et al., 2020; Ohshima and Morii, 2021). Metabolic alterations lead to enhanced uptake and utilization of glucose and amino acid nutrients from a nutrient-deficient environment and increased use of glycolysis/TCA cycle intermediates for macromolecular biosynthesis. Cancer cell metabolism is governed by the Warburg effect: aerobic glycolysis with the increased rate of glycolysis and lactate production despite exposure to ambient oxygen. It is now clear that mitophagy is an important cellular mechanism that facilitates the metabolic switch to a glycolytic phenotype (Naik et al., 2019). Both canonical and non-canonical mitophagy contribute to alterations of bioenergetics and the metabolome, enhancing cellular developmental capability. Metabolic transformation of tumor cells, such as increased aerobic glycolysis or reduced oxidative phosphorylation, is controlled by altered expression and activity of several key enzymes involved in metabolism (Pavlova and Thompson, 2016). Mitophagy may play a critical role in this reprogramming by eliminating dysfunctional and damaged mitochondria. The turnover of mitochondria activates mitochondrial biogenesis to generate new mitochondrial mass that more effectively responds to nutrient stress (Drake et al., 2017). Hypoxia-inducible factor 1 (HIF-1) is a key factor that coordinates mitophagy and mitochondria turnover by regulating gene expression. In response to chronic hypoxia, HIF-1 initiates mitophagy by regulating the expression of mitophagy receptors BNIP3 and BNIP3L. The reduction of mitochondrial mass decreases total oxygen consumption of tumor cells and enhances cell survival under hypoxic stress (Vara-Perez et al., 2019; Figure 3).
FIGURE 3
Mitophagy is also required for self-renewal and maintenance of stemness (Figure 3). CSCs exhibit enhanced potential for metastatic spread and contribute to the resistance to anticancer therapies. CSCs have unique metabolic signatures and heavily depend on the coordination of mitochondrial oxidative phosphorylation and mitophagy to maintain CSC phenotypes, including glycolytic reprogramming, epithelial-to-mesenchymal transition, survival after chemotherapeutic challenge, and increased formation of ROS (Sessions and Kashatus, 2021). Liu et al. (2017) reported that mitophagy was a positive regulator of hepatic CSCs. Active p53 binds to the promoter of NANOG and prevents OCT4 and SOX2 transcription factors from activating that stemness gene, leading to a reduction in hepatoma stem cells. When mitophagy is activated, p53 is phosphorylated at serine-392 by PINK1, a mitophagy-associated kinase, and is degraded by a mitophagy-dependent mechanism. Thus, mitophagy maintains hepatic CSCs by targeting the activities of tumor suppressor p53. Katajisto et al. (2015) found that mitophagy may play a critical role in regulating stemness by isolating and eliminating old mitochondria during asymmetric division of stem cells. Collectively, these studies demonstrate that mitophagy plays a positive role in the metabolic transition of tumors and the maintenance of phenotype of CSCs.
The Role of Mitophagy in Tumor Progression
Mitophagy is a multi-pathway-regulated and multi-step process to degrade dysfunctional and damaged mitochondria. As a result, the role of mitophagy in tumor progression is very complex and depends on the type and stage of the tumor, as well as the specific mitophagy component molecules examined. Chourasia et al. (2015) reported that BNIP3 is most commonly deleted in patients with triple-negative breast cancer (TNBC). Similarly, BNIP3 functioned as a tumor suppressor in a MMTV-PyMT mouse mammary tumor model. Lack of Bnip3 caused an increment in tumor growth, invasiveness, and lung metastases. The role of BNIP3-dependent mitophagy, however, is controversial, partly depending on alternative pre-mRNA splicing of BNIP3. Normal cells express the full-length BNIP3, which promotes cell death. However, human adenocarcinoma cells preferentially express the truncated BNIP3 variant Bnip3Δex that lacks exon 3. The truncated Bnip3Δex3 isoform promotes tumor survival (Gang et al., 2015). It is also noteworthy that the move toward glycolytic metabolism upregulates the expression of the truncated Bnip3Δex3 isoform. Thus, BNIP3-mediated mitophagy may have a complex role in tumor growth and metastasis, depending on tumor type, alternative gene splicing regulation, and metabolic activity in cancer cells (Ferro et al., 2020).
Some studies suggested that PINK1 and PARK2 may function as tumor suppressors. Li C. et al. (2018) showed that expression of PARK2 was associated with improved survival in patients with pancreatic cancer. Using a mutant Kras-driven spontaneous pancreatic tumorigenesis model, they found that genetic deletion of Parkin or Pink1 promoted the development of pancreatic tumorigenesis. PARK2 is a component in the p53 tumor suppressor pathway. Lack of PARK2 induced the Warburg effect with increased glycolysis and ROS production, potentially enhancing tumorigenesis (Zhang et al., 2011). FUNDC1 is a mitophagy receptor that mediates mitophagy in response to hypoxia. Li W. et al. (2019) showed that FUNDC1 was upregulated in human hepatocellular carcinoma (HCC) tissues. Using a hepatocyte-specific Fundc1-knockout mouse model, they demonstrated that Fundc1 depletion enhanced the development and progression of HCC through the mitophagy-inflammasome pathway.
Overall, most mitophagy receptors or regulators are involved in cancer, but whether they function as tumor promoters or suppressors depends on tumor type and tumor microenvironment. Consistent with this dual role of mitophagy in tumors, mitophagy pathway proteins may be either overexpressed or downregulated in cancer (Table 1).
TABLE 1
| Mitophagy protein | Expression levels in cancer | Cancer type | References |
| Parkin | Increased | Melanoma | Lee Y. S. et al., 2018 |
| Decreased | Lung cancer; breast cancer; glioma; pancreatic ductal adenocarcinoma; and colon cancer | Poulogiannis et al., 2010; Tay et al., 2010; D’Amico et al., 2015; Maugeri et al., 2015; Li C. et al., 2018 | |
| Pink1 | Increased | Lung cancer; and esophageal squamous cell carcinoma | Yamashita et al., 2017; Liu et al., 2018 |
| Decreased | Colorectal cancer; glioblastoma; and pancreatic ductal adenocarcinoma | Poulogiannis et al., 2010; Agnihotri et al., 2016; Li C. et al., 2018 | |
| BNIP3 | Increased | Prostate cancer; uveal melanoma; and renal carcinoma | Chen et al., 2010; Macher-Goeppinger et al., 2017; Jiang et al., 2018 |
| Decreased | Triple-negative breast cancer; and colorectal cancer | Bacon et al., 2007; Chourasia et al., 2015 | |
| BNIP3L | Increased | Pancreatic ductal adenocarcinoma | Humpton et al., 2019 |
| Decreased | Lung cancer | Sun et al., 2004 | |
| FUNDC1 | Increased | Laryngeal cancer; cervical cancer; and breast cancer | Hou et al., 2017; Hui et al., 2019; Wu L. et al., 2019 |
| Decreased | Hepatocellular carcinoma | Li W. et al., 2019 |
The expression of mitophagy proteins in different cancer types.
LncRNAs Emerge as New Epigenetic Regulators of Mitophagy in Cancers
LncRNAs Deliver Epigenetic Messages Between Mitochondria and the Nucleus
Mitochondria and the nucleus contain distinct genomes. Mitochondria participate in crucial cellular processes involved in energy harvesting, intermediate metabolism, and apoptosis. Consequently, mitochondria must communicate and coordinate precisely with the nuclear genome to ensure proper cellular function and energy homeostasis (Barcena et al., 2018; English et al., 2020). This mitochondrial-nuclear crosstalk is coordinated by anterograde (from the nucleus to mitochondria) and retrograde (from mitochondria to nucleus) signals, ensuring that cells maintain homeostasis under basal conditions while enabling adaptation to various stressors (Noh et al., 2016; Quiros et al., 2016; Singh et al., 2017). In response to mitochondrial stressors, retrograde signals induce specific nuclear expression of proteins that migrate into the mitochondria to resolve these perturbations.
Recent studies suggest that lncRNAs may function as novel retrograde and anterograde signal molecules in this mitochondria-nuclear crosstalk (Dong et al., 2017; Zhao et al., 2018). LncRNAs are encoded not only from the nuclear genome but also from the mitochondrial genome (mtDNA). LncRNAs encoded by the mitochondrial genome can be exported into the cytoplasm and nucleus, where they can have pleiotropic interactions. Similarly, lncRNAs synthesized from the nuclear genome can be shuttled into the mitochondria, where they can modulate gene transcription or mitochondrial metabolism (Rackham et al., 2011; Noh et al., 2016).
The mitochondrial genome codes for 13 mRNAs, 22 tRNAs, and 2 rRNAs, producing proteins that participate in oxidative phosphorylation. In addition, several mtDNA-encoded lncRNAs have recently been characterized, including lncND5, lncND6, and lncCyt B (Rackham et al., 2011), and their expression is cell- and tissue-specific, suggesting an important role in the regulation of mitochondrial gene expression. Together, at least 18 mitochondria-associated ncRNAs have been identified, and they play critical roles in manipulating mitochondrial functions, altering metabolic reprogramming, mitochondrial genome transcription, stress signal transmission, and mitochondria-associated apoptosis (Zhao et al., 2018).
Using RNA-FISH with MitoTracker staining, Zhao et al. (2019) examined the mitochondrial localization of lncRNAs, and found that some nuclear genome-transcribed lncRNAs, like the oncogenic lncRNA MALAT1, were aberrantly translocated to the mitochondria of HCC cells; in normal liver cells, this lncRNA was found predominantly in the nucleus. MALAT1 acts as an anterograde signal to regulate mitochondrial function in HepG2 cells. In contrast, the mitochondria-encoded lncRNA lncCytB acts as a retrograde signal in this mitochondria-nuclear crosstalk. This is in a sharp contrast to normal hepatic HL7702 cells, where lncCytB was primarily distributed in the mitochondria. Thus, aberrant shuttling of lncRNAs, whether nuclear-encoded or mitochondria-encoded, may be involved in the regulation of metabolic reprogramming in cancer cells.
LncRNAs Regulate Mitochondrial Metabolic Reprogramming in Cancers
The role of lncRNAs as crucial regulators of the Warburg effect in cancer has recently been investigated (Shankaraiah et al., 2018; Cruz-Gil et al., 2020; Liu et al., 2021). LncRNA EPB41L4A-AS1, a downstream target of p53, was downregulated in several human cancers, including breast cancer, and its downregulation was associated with poor survival. Depletion of EPB41L4A-AS1 expression induced the Warburg effect and increased aerobic glycolysis and glutamine metabolism through the HIF-1α pathway (Liao et al., 2019). FILNC1 (FoxO-induced lncRNA-1), an energy stress-induced lncRNA, was downregulated in renal cancer. Under glucose starvation, FILNC1-depleted cells exhibited enhanced glucose uptake and lactate production, probably through the c-Myc network (Xiao et al., 2017). LncRNA Ftx, derived from the X-inactivation center on the X chromosome, was overexpressed in HCC tissues, and its expression was associated with malignant clinicopathological characteristics in HCC patients. Ftx was shown to promote the Warburg effect and enhance tumor progression through the PPARγ pathway (Li X. et al., 2018). p21, a well-known p53-inducible lncRNA, is also a key regulator of the Warburg effect and is essential for hypoxia-induced glycolysis (Yang et al., 2014). While these latter two lncRNAs are not located in the mitochondria, we believe that a wide variety of mitochondrial-associated lncRNAs play important roles in controlling energy production as well as mtDNA, providing potent therapeutic targets and diagnostic markers for cancer.
In hepatoma cells, the nuclear genome-encoded lncRNA MALAT1 is abnormally transported into the mitochondria. Using an RNA reverse transcription-associated trap sequencing (RAT-seq) technology, Zhao et al. (2021) profiled the target binding sites of MALAT1 in the mitochondrial genome. They showed that mitochondrial MALAT1 lncRNA molecules were able to bind to multiple targets in mtDNA, including COX2, which encodes a subunit of the cytochrome c oxidase complex responsible for electron transfer in the mitochondrial respiratory chain. Depletion of the mitochondria-enriched MALAT1, either using shRNA or LwaCas13a-BN-MLS mitochondrial RNA targeting, altered mtDNA CpG methylation and induced multiple abnormalities in mitochondrial function and energy metabolism. Their findings suggest that lncRNA MALAT1 may function as a novel nucleus-mitochondria epigenetic messenger. By shuttling between the nucleus and mitochondria, it epigenetically regulates mitochondrial metabolism in hepatoma cells.
Interplay of Mitophagy and LncRNAs in the Maintenance of CSCs
Hypoxia is common inducer of mitophagy, and CSCs can adapt to the hypoxic tumor microenvironment through mitophagy and maintain their stemness. In addition, CSCs can use mitophagy to maintain their own self-renewal ability. The expression of HIFs after hypoxia can directly or indirectly stimulate the maintenance of stemness markers, such as OCT4, SOX2, etc (Zaarour et al., 2021). LncRNA XIST can increase the sensitivity of breast CSCs to chemotherapy drugs by regulating KMT2C (Huang et al., 2020). In liver cancer, lncRNA HDAC2 promotes the proliferation and self-renewal of liver CSCs by activating the Hedgehog signaling pathway (Wu J. et al., 2019).
Exosomal lncRNAs are key factors that mediate extracellular communication in the tumor microenvironment. DOCK9-as2 is an exosomal lncRNA, and its down-regulation reduces the proliferation, migration, invasion, epithelial-mesenchymal (EMT), and stemness of papillary thyroid carcinoma cells (Dai et al., 2020). In both in vivo and in vitro studies, Gao et al. (2021) demonstrated that exosomal lncRNA UCA1 promoted self-renewal and differentiation of CD133 + cervical cancer cells through the micro-rRNA-122-5P/SOX2 axis. Silencing of UCA1 reduced cell proliferation and invasion. Similarly, depletion of lncRNA HotairM1 promotes self-renewal of CSCs through the HOXA1-Nanog loop (Li F. et al., 2020). These observations suggest that the crosstalk between hypoxia-driven HIFs, lncRNAs, and mitophagy plays a critical role in maintaining the stemness of CSCs.
LncRNAs Are New Epigenetic Players in Mitophagy
LncRNAs are involved in the regulation of autophagy as well as autophagy-associated drug resistance. LncRNA HULC is highly upregulated in liver cancer and other malignancies. HULC overexpression is associated with poor overall survival and metastasis in cancers (Ding et al., 2019; Ghafouri-Fard et al., 2020). Knockdown of HULC suppresses autophagy and reduces cisplatin resistance by targeting downstream protein FoxM1 in drug-resistant gastric cancer cells (Xin et al., 2019). Another study reported that HULC triggered autophagy by stabilizing Sirt1, attenuating the sensitivity of HCC cells to chemotherapy (Xiong et al., 2017). Sun et al. (2017) found that lncRNA HOTAIR regulated cisplatin-resistance and autophagy by targeting Beclin-1, MDR, and P-GP in endometrial cancer cells. Finally, Liu C. et al. (2020) showed that lncRNA RMST was overexpressed in clinical glioma samples. RMST inhibited autophagy in glioma cells by inducing SUMO1 modification at K333 of FUS. SUMOylation of FUS promotes the degradation of ATG4D, a regulator of autophagy. More advances in the regulation of autophagy by lncRNAs in tumors are summarized in Table 2.
TABLE 2
| LncRNAs | Cancer type | Expression level in cancer | The effect of lncRNA on autophagy | The effect of lncRNA on chemoresistance | References |
| GBCDRlnc1 | Gallbladder cancer | Increased | Promoted | Promoted | Cai et al., 2019 |
| DCST1-AS1 | Hepatocellular carcinoma | Increased | Promoted | / | Li J. et al., 2019 |
| CCAT1 | Hepatocellular carcinoma | Increased | Promoted | / | Guo et al., 2019 |
| HAGLROS | Gastric cancer | Increased | Inhibited | / | Chen et al., 2018 |
| NEAT1 | Hepatocellular carcinoma | Increased | Promoted | Promoted | Li X. et al., 2020 |
| NEAT1 | Colorectal cancer | Increased | Promoted | Promoted | Liu F. et al., 2020 |
| PVT1 | Pancreatic ductal adenocarcinoma | Increased | Promoted | / | Huang et al., 2018 |
| NBR2 | Hepatocellular carcinoma | Decreased | Inhibited | / | Sheng et al., 2021 |
| ATB | Hepatocellular carcinoma | Increased | Promoted | / | Wang C. Z. et al., 2019 |
| DANCR | Hepatocellular carcinoma | Increased | Promoted | / | Wang X. et al., 2020 |
| ZNNT1 | Uveal melanoma | Decreased | Promoted | / | Li P. et al., 2020 |
| SNHG7 | Neuroblastoma | Promoted | Wang S. Y. et al., 2020 | ||
Regulation of autophagy by LncRNA in different cancer types.
The crosstalk between autophagy and lncRNA may play a vital role in tumor progression. Using TCGA and CGGA databases, Xu et al. (Luan et al., 2019) analyzed 988 diffuse glioma patients and found that these patients were divided into two clusters with different prognostic outcomes based on the autophagy-related lncRNAs (ARLs) score. The ARLs signatures constructed by the authors showed good accuracy in predicting the prognosis of glioma patients. The ARL score was significantly elevated in the malignant subtype of glioma, and a high ARL score suggested a poor prognosis. A high ARL score indicated high infiltration of macrophages and neutrophils, thus serving as a promising prognostic biomarker for glioma patients. Using similar data analysis approaches, Li et al. (2021) found 11 ARLs in association with breast cancer prognosis. These 11 ARLs may become potential targets for autophagy-related targeted therapy.
The mechanism underlying the role of lncRNAs in the regulation of mitophagy remains unclear. Zhao et al. (2021) showed that the nuclear genome-encoded lncRNA MALAT1 acts as a new epigenetic messenger by shuttling to the mitochondria, where it regulates mitophagy (Figure 4). Knockdown of MALAT1 exhibited a significant decrease in mitophagy events. Mitophagy proteins, particularly PINK1, P62, NDP52, BNIP3, and LC3, were significantly downregulated in MALAT1-depleted HCC cells. This study proved for the first time that the lncRNA encoded by the nuclear genome might act as a new epigenetic player and regulate mitochondrial metabolism through mitophagy. In a second study, Xiang et al. (2021) observed the overexpression of PINT87, a peptide encoded by p53-induced transcript LINC-PINT, in senescent HCC cells. Overexpression of PINT87 inhibited mitophagy by directly binding to FOXM1 and blocking the transcription of PHB2, a crucial IMM receptor for Parkin-induced mitophagy.
FIGURE 4
Application and Challenges of Modulating Mitophagy in Anti-Cancer Therapies
Inhibition of Mitophagy to Enhance Sensitivity to Chemotherapy
Many anti-cancer drugs directly or indirectly cause damage to the mitochondria. Thus, the removal of dysfunctional mitochondria by mitophagy may alter their overall effectiveness (Bishop and Bradshaw, 2018). The induction of mitophagy during anti-tumor therapy may alter its cytotoxicity, leading to treatment resistance. ARIH1, an E3 Ub ligase belonging to the RING-between-RING (RBR) family, is overexpressed in many cancer cells. As a key regulator of mitophagy, ARIH1 activates the PINK1-dependent mitophagy that promotes the resistance to cisplatin chemotherapy (Villa et al., 2017). There is a tight regulatory crosstalk between apoptosis, mitophagy, endoplasmic reticulum stress, and mitochondrial dynamics/biogenesis in determining the fate of cells in chemotherapy (Abdrakhmanov et al., 2019). Stimulation of mitophagy with the protonophore carbonyl cyanide-m chlorophenylhydrazone (CCCP) inhibited the apoptosis induced by cisplatin, particularly at the high dose in HCT116 cells. On the other hand, inhibition of mitophagy enhanced the apoptotic response.
TMZ-POH, a novel conjugation analog of alkylating anticancer agent temozolomide, impaired mitophagy flux in non-small cell lung cancer (NSCLC) cells by inducing lysosomal dysfunction and hampering autophagosome-lysosome fusion. In radiotherapy, inhibition of mitophagy by TMZ-POH sensitizes cancer cells to irradiation-induced apoptosis (Chang et al., 2018). PINK1/Parkin-mediated mitophagy plays a critical role in hypoxia-induced radioresistance. Inhibition of this mitophagy pathway by a hypoxia-targeting p53 fusion protein, consisting of p53, TAT, and HIF-1α minimum oxygen-dependent degradation domain, sensitizes cancer cells in response to radiotherapy both in vitro and in vivo (Zheng et al., 2015). Collectively, these data support the pro-survival role of mitophagy in chemo/radiotherapy.
Inhibition of Mitophagy to Adjust Drug Resistance in CSCs
Cancer stem cells are known to be drug-resistant. To study the role of mitophagy in CSC-mediated drug resistance, Yan et al. (2017) isolated CSCs carrying the CD133+/CD44+ marker from human colorectal cancer cells that were resistant to doxorubicin. Treatment with doxorubicin significantly upregulated the BNIP3L mitophagy pathway. Inhibition of this mitophagy pathway significantly enhanced the sensitivity to doxorubicin in CSCs. Thus, mitophagy may contribute to drug resistance in CSCs.
To examine the mechanisms underlying chemoresistance and its correlation with stemness, Naik et al. (2018) established cisplatin−resistant oral squamous cell carcinoma cells with CSC-like features. They found that mitophagy flux was significantly higher in cisplatin-resistant oral cancer cells than that in their parental counterparts, suggesting that mitophagy is responsible for chemoresistance in oral cancer. Inhibition of autophagy effectively downregulated the stemness and inhibited chemoresistance.
Nucleus-Encoded LncRNAs as New Therapeutic Targets of Mitophagy
Long non-coding RNAs may promote tumor cell survival and reduce sensitivity of cancer cells to chemo- and radiotherapy by regulating mitophagy. Thus, targeting lncRNAs may be an appropriate approach to render cancer cells sensitive to chemotherapy. Cai et al. (2019) found that lncRNA GBCDRlnc1 (gallbladder cancer drug resistance-associated lncRNA1), a key regulator of chemotherapy resistance, is upregulated in gallbladder cancer. Depletion of this lncRNA inhibited autophagy and enhanced the sensitivity of gallbladder cancer cells to doxorubicin. Their findings established that targeting the chemoresistant driver GBCDRlnc1 might be an attractive therapeutic approach for the treatment of advanced gallbladder cancer.
Long non-coding RNA H19 plays an important role in cell proliferation, metastasis, and chemotherapy resistance. Wang J. et al. (2019) showed that H19 was significantly upregulated in breast cancer cell lines and tumor tissues that were resistant to tamoxifen treatment. Silencing H19 significantly inhibited autophagy and sensitized tumor cells to tamoxifen in vitro and in vivo. In contrast, overexpression of H19 triggered autophagy in tamoxifen-sensitive cells and recapitulated the characteristics of tamoxifen resistance. Another study with lncRNA KCNQ1OT1 in colon cancer patients found that KCNQ1OT1 enhanced the chemotherapy resistance of colon cancer by activating autophagy through the miR-34a-Atg4B axis (Li Y. et al., 2019). Thus, targeting lncRNAs to regulate autophagy in tumors may be a promising therapeutic approach.
Disruption of the mitochondria-nuclear crosstalk network is a key event in many human diseases, including cancer (Mello et al., 2019). As critical regulatory components of this mitochondria-nuclear crosstalk, lncRNAs are also often dysregulated, including aberrant expression and shuttling between the nucleus and mitochondria. For example, the nuclear-encoded MALAT1 can be shuttled to mitochondria, where it regulates the mitochondrial functions and mitophagy (Zhao et al., 2021). Thus, targeting a component of the dysregulated lncRNA-mitochondria-nuclear network may provide an ideal method to combat some malignancies. Novel targets include the nuclear expression of the lncRNA, nuclear-mitochondria shuttling, lncRNA binding to mitochondria, and downstream signals of the mitophagy pathway.
Conclusion
Targeting mitophagy may present a new approach to develop anti-cancer therapies. However, currently we know very little about the precise mechanisms underlying the regulation of mitophagy in human tumors. Thus, it is critical to identify the role of mitophagy as well as the key regulatory components of each mitophagy pathway in tumor progression. More importantly, we must accurately define specific mitophagy regulators involved in radio- and chemoresistance in order to develop a precision medicine approach to target a specific component or a key regulator in mitophagy, rather than using inhibitors to target general mitophagy. A more comprehensive understanding of the key regulators of mitophagy in tumor progression may guide the development of novel therapeutics to treat some cancers (Bishop and Bradshaw, 2018). At the same time, it is also critical to understand the extent to which normal cells can tolerate these mitophagy inhibitors, so that drugs targeting mitophagy will not damage normal cells. More importantly, we should identify key regulatory factors that aberrantly regulate mitophagy in cancer cells to improve the precision of anti-tumor therapy.
The roles of mitochondria-associated lncRNAs in mitophagy are just beginning to be explored. As epigenetic regulatory factors, lncRNAs play important roles in intracellular environmental homeostasis, including mitophagy. Notably, some nucleus/mitochondria-shuttling lncRNAs are involved in the regulation of cancer metabolic reprogramming and the stemness maintenance of CSCs. After being transported to mitochondria, the nuclear-encoded lncRNAs not only epigenetically regulate mitochondrial metabolism, but also play a vital role in apoptosis and mitophagy. Therefore, precisely targeting mitophagy-related lncRNAs in cancer may become a very promising and attractive strategy for future tumor therapy with fewer toxic and side effects.
Since lncRNAs regulate mitophagy through multiple signaling pathways and mechanisms, more studies are needed before we target mitophagy-associated lncRNAs for precision medicine in cancer. For example, how are these nuclear lncRNAs transported into mitochondria? Can we interrupt this mitochondria-nuclear transportation to target mitophagy? What are the targets of these lncRNAs in mitochondria? How do these lncRNAs regulate mitophagy? A greater understanding of lncRNAs in this mitochondria-nuclear crosstalk network may help us develop novel lncRNA-based therapeutic approaches for malignancies that currently have few curative options.
Publisher’s Note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Statements
Author contributions
YL wrote the manuscript. J-FH, JC, and WL supervised and funded the project. J-FH and AH edited the manuscript. All authors contributed to the article and approved the submitted version.
Funding
This work was supported by the National Key R&D Program of China (2018YFA0106902), the National Natural Science Foundation of China (82050003, 81900701, 31430021, 81874052, 81672275, 31871297, 81670143, 81900701, and 32000431), the Key Project of Chinese Ministry of Education grant (311015), the National Basic Research Program of China (973 Program; 2015CB943303), the Nation Key Research and Development Program of China grant (2016YFC13038000), Research on Chronic Non-communicable Diseases Prevention and Control of National Ministry of Science and Technology (2016YFC1303804), the National Health Development Planning Commission Major Disease Prevention and Control of Science and Technology Plan of Action, Cancer Prevention and Control (ZX-07-C2016004), the Natural Science Foundation of Jilin Province (20200801046GH, 20150101176JC, 20180101117JC, and 20130413010GH), the Provincial Science Fund of Jilin Province Development and Reform Commission (2014N147 and 2017C022), the 10th Youth Fund of First Hospital of Jilin University (JDYY 102019034), California Institute of Regenerative Medicine (CIRM) grant (RT2-01942), and the Biomedical Research Service of the Department of Veterans Affairs (BX002905).
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
References
1
AbdrakhmanovA.KulikovA. V.LuchkinaE. A.ZhivotovskyB.GogvadzeV. (2019). Involvement of mitophagy in cisplatin-induced cell death regulation.Biol. Chem.400161–170. 10.1515/hsz-2018-0210
2
AgnihotriS.GolbournB.HuangX.RemkeM.YoungerS.CairnsR. A.et al (2016). PINK1 is a negative regulator of growth and the Warburg effect in Glioblastoma.Cancer Res.764708–4719. 10.1158/0008-5472.can-15-3079
3
AnandA.PandiG. (2021). Noncoding RNA: an insight into chloroplast and mitochondrial gene expressions.Life11:49. 10.3390/life11010049
4
BaconA. L.FoxS.TurleyH.HarrisA. L. (2007). Selective silencing of the hypoxia-inducible factor 1 target gene BNIP3 by histone deacetylation and methylation in colorectal cancer.Oncogene26132–141. 10.1038/sj.onc.1209761
5
BarcenaC.MayoralP.QuirosP. M. (2018). Mitohormesis, an Antiaging paradigm.Int. Rev. Cell Mol. Biol.34035–77. 10.1016/bs.ircmb.2018.05.002
6
BilliaF.HauckL.KonecnyF.RaoV.ShenJ.MakT. W. (2011). PTEN-inducible kinase 1 (PINK1)/Park6 is indispensable for normal heart function.Proc. Natl. Acad. Sci. U.S.A.1089572–9577. 10.1073/pnas.1106291108
7
Bin-UmerM. A.McLaughlinJ. E.ButterlyM. S.McCormickS.TumerN. E. (2014). Elimination of damaged mitochondria through mitophagy reduces mitochondrial oxidative stress and increases tolerance to trichothecenes.Proc. Natl. Acad. Sci. U.S.A.11111798–11803. 10.1073/pnas.1403145111
8
BishopE.BradshawT. D. (2018). Autophagy modulation: a prudent approach in cancer treatment?Cancer Chemother. Pharmacol.82913–922. 10.1007/s00280-018-3669-6
9
CaiQ.WangS.JinL.WengM.ZhouD.WangJ.et al (2019). Long non-coding RNA GBCDRlnc1 induces chemoresistance of gallbladder cancer cells by activating autophagy.Mol. Cancer18:82.
10
ChangM.SongX.GengX.WangX.WangW.ChenT. C.et al (2018). Temozolomide-Perillyl alcohol conjugate impairs Mitophagy flux by inducing lysosomal dysfunction in non-small cell lung Cancer cells and sensitizes them to irradiation.J. Exp. Clin. Cancer Res.37:250.
11
ChangX.ZhangW.ZhaoZ.MaC.ZhangT.MengQ.et al (2020). Regulation of mitochondrial quality control by natural drugs in the treatment of cardiovascular diseases: potential and advantages.Front. Cell Dev. Biol.8:616139. 10.3389/fcell.2020.616139
12
ChenJ. F.WuP.XiaR.YangJ.HuoX. Y.GuD. Y.et al (2018). STAT3-induced lncRNA HAGLROS overexpression contributes to the malignant progression of gastric cancer cells via mTOR signal-mediated inhibition of autophagy.Mol. Cancer17:6.
13
ChenX.ChenS.YuD. (2020). Metabolic reprogramming of chemoresistant cancer cells and the potential significance of metabolic regulation in the reversal of cancer chemoresistance.Metabolites10:289. 10.3390/metabo10070289
14
ChenX.GongJ.ZengH.ChenN.HuangR.HuangY.et al (2010). MicroRNA145 targets BNIP3 and suppresses prostate cancer progression.Cancer Res.702728–2738. 10.1158/0008-5472.can-09-3718
15
ChourasiaA. H.TracyK.FrankenbergerC.BolandM. L.SharifiM. N.DrakeL. E.et al (2015). Mitophagy defects arising from BNip3 loss promote mammary tumor progression to metastasis.EMBO Rep.161145–1163. 10.15252/embr.201540759
16
ClarkS. L.Jr. (1957). Cellular differentiation in the kidneys of newborn mice studies with the electron microscope.J. Biophys. Biochem. Cytol.3349–362. 10.1083/jcb.3.3.349
17
CornelissenT.VilainS.VintsK.GounkoN.VerstrekenP.VandenbergheW. (2018). Deficiency of parkin and PINK1 impairs age-dependent mitophagy in Drosophila.eLife7:e35878.
18
Cruz-GilS.FernandezL. P.Sanchez-MartinezR.Gomez de CedronM.Ramirezet al (2020). Non-coding and regulatory RNAs as epigenetic remodelers of fatty acid homeostasis in cancer.Cancers12:2890. 10.3390/cancers12102890
19
DaiW.JinX.HanL.HuangH.JiZ.XuX.et al (2020). Exosomal lncRNA DOCK9-AS2 derived from cancer stem cell-like cells activated Wnt/β-catenin pathway to aggravate stemness, proliferation, migration, and invasion in papillary thyroid carcinoma.Cell Death Dis.11:743.
20
D’AmicoA. G.MaugeriG.MagroG.SalvatorelliL.DragoF.D’AgataV. (2015). Expression pattern of parkin isoforms in lung adenocarcinomas.Tumour Biol.365133–5141. 10.1007/s13277-015-3166-z
21
De FalcoF.GentileI.CerinoP.CutarelliA.CatoiC.RopertoS. (2020). Prohibitin 2 is involved in Parkin-mediated mitophagy in urothelial cells of cattle infected with bovine papillomavirus.Pathogens9:621. 10.3390/pathogens9080621
22
DingY.SunC.LiJ.HuL.LiM.LiuJ.et al (2019). The significance of long non-coding RNA HULC in predicting prognosis and metastasis of cancers: a meta-analysis.Pathol. Oncol. Res.25311–318. 10.1007/s12253-017-0351-y
23
DongY.YoshitomiT.HuJ. F.CuiJ. (2017). Long noncoding RNAs coordinate functions between mitochondria and the nucleus.Epigenetics Chromatin10:41.
24
DrakeL. E.SpringerM. Z.PooleL. P.KimC. J.MacleodK. F. (2017). Expanding perspectives on the significance of mitophagy in cancer.Semin. Cancer Biol.47110–124. 10.1016/j.semcancer.2017.04.008
25
EnglishJ.SonJ. M.CardamoneM. D.LeeC.PerissiV. (2020). Decoding the rosetta stone of mitonuclear communication.Pharmacol. Res.161:105161. 10.1016/j.phrs.2020.105161
26
FerroF.ServaisS.BessonP.RogerS.DumasJ. F.BrissonL. (2020). Autophagy and mitophagy in cancer metabolic remodelling.Semin. Cell Dev. Biol.98129–138. 10.1016/j.semcdb.2019.05.029
27
FriedmanJ. R.NunnariJ. (2014). Mitochondrial form and function.Nature505335–343. 10.1038/nature12985
28
GangH.DhingraR.LinJ.HaiY.AvivY.MarguletsV.et al (2015). PDK2-mediated alternative splicing switches Bnip3 from cell death to cell survival.J. Cell Biol.2101101–1115. 10.1083/jcb.201504047
29
GaoZ.WangQ.JiM.GuoX.LiL.SuX. (2021). Exosomal lncRNA UCA1 modulates cervical cancer stem cell self-renewal and differentiation through microRNA-122-5p/SOX2 axis.J. Transl. Med.19:229.
30
Ghafouri-FardS.EsmaeiliM.TaheriM.SamsamiM. (2020). Highly upregulated in liver cancer (HULC): an update on its role in carcinogenesis.J. Cell. Physiol.2359071–9079. 10.1002/jcp.29765
31
GongG.SongM.CsordasG.KellyD. P.MatkovichS. J.DornG. W.II (2015). Parkin-mediated mitophagy directs perinatal cardiac metabolic maturation in mice.Science350:aad2459. 10.1126/science.aad2459
32
GottliebR. A.BernsteinD. (2015). METABOLISM, Mitochondria shape cardiac metabolism.Science3501162–1163. 10.1126/science.aad8222
33
GreeneA. W.GrenierK.AguiletaM. A.MuiseS.FarazifardR.HaqueM. E.et al (2012). Mitochondrial processing peptidase regulates PINK1 processing, import and Parkin recruitment.EMBO Rep.13378–385. 10.1038/embor.2012.14
34
GuoJ.MaY.PengX.JinH.LiuJ. (2019). LncRNA CCAT1 promotes autophagy via regulating ATG7 by sponging miR-181 in hepatocellular carcinoma.J. Cell. Biochem.12017975–17983. 10.1002/jcb.29064
35
HarperJ. W.OrdureauA.HeoJ. M. (2018). Building and decoding ubiquitin chains for mitophagy.Nat. Rev. Mol. Cell Biol.1993–108. 10.1038/nrm.2017.129
36
HeF.HuangY.SongZ.ZhouH. J.ZhangH.PerryR. J.et al (2021). Mitophagy-mediated adipose inflammation contributes to type 2 diabetes with hepatic insulin resistance.J. Exp. Med.218:e20201416.
37
HouH.ErP.ChengJ.ChenX.DingX.WangY.et al (2017). High expression of FUNDC1 predicts poor prognostic outcomes and is a promising target to improve chemoradiotherapy effects in patients with cervical cancer.Cancer Med.61871–1881. 10.1002/cam4.1112
38
HuangF.ChenW.PengJ.LiY.ZhuangY.ZhuZ.et al (2018). LncRNA PVT1 triggers Cyto-protective autophagy and promotes pancreatic ductal adenocarcinoma development via the miR-20a-5p/ULK1 Axis.Mol. Cancer17:98.
39
HuangR.ZhuL.ZhangY. (2020). XIST lost induces ovarian cancer stem cells to acquire taxol resistance via a KMT2C-dependent way.Cancer Cell Int.20:436.
40
HuiL.WuH.WangT. W.YangN.GuoX.JangX. J. (2019). Hydrogen peroxide-induced mitophagy contributes to laryngeal cancer cells survival via the upregulation of FUNDC1.Clin. Transl. Oncol.21596–606. 10.1007/s12094-018-1958-5
41
HumptonT. J.AlagesanB.DeNicolaG. M.LuD.YordanovG. N.LeonhardtC. S.et al (2019). Oncogenic KRAS induces NIX-mediated mitophagy to promote pancreatic cancer.Cancer Discov.91268–1287. 10.1158/2159-8290.cd-18-1409
42
JiangZ.YuF.LiM. (2018). Upregulation of BCL2 19 kD Protein-Interacting Protein 3 (BNIP3) is Predictive of Unfavorable Prognosis in Uveal Melanoma.Med. Sci. Monit.244711–4717. 10.12659/msm.907679
43
KatajistoP.DöhlaJ.ChafferC. L.PentinmikkoN.MarjanovicN.IqbalS.et al (2015). Stem cells. Asymmetric apportioning of aged mitochondria between daughter cells is required for stemness.Science348340–343. 10.1126/science.1260384
44
KazlauskaiteA.KondapalliC.GourlayR.CampbellD. G.RitortoM. S.HofmannK.et al (2014). Parkin is activated by PINK1-dependent phosphorylation of ubiquitin at Ser65.Biochem. J.460127–139. 10.1042/bj20140334
45
KeP. Y. (2020). Mitophagy in the pathogenesis of liver diseases.Cells9:831. 10.3390/cells9040831
46
KouroumalisE.VoumvourakiA.AugoustakiA.SamonakisD. N. (2021). Autophagy in liver diseases.World J. Hepatol.136–65.
47
KoyanoF.OkatsuK.KosakoH.TamuraY.GoE.KimuraM.et al (2014). Ubiquitin is phosphorylated by PINK1 to activate parkin.Nature510162–166. 10.1038/nature13392
48
KuznetsovA. V.JavadovS.GrimmM.MargreiterR.AusserlechnerM. J.HagenbuchnerJ. (2020). Crosstalk between mitochondria and cytoskeleton in cardiac cells.Cells9:222. 10.3390/cells9010222
49
LeeJ. J.AndreazzaS.WhitworthA. J. (2020). The STING pathway does not contribute to behavioural or mitochondrial phenotypes in Drosophila Pink1/parkin or mtDNA mutator models.Sci. Rep.10:2693.
50
LeeJ. J.Sanchez-MartinezA.Martinez ZarateA.BenincáC.MayorU.ClagueM. J.et al (2018). Basal mitophagy is widespread in Drosophila but minimally affected by loss of Pink1 or parkin.J. Cell Biol.2171613–1622. 10.1083/jcb.201801044
51
LeeY.LeeH. Y.HannaR. A.GustafssonÅ. B. (2011). Mitochondrial autophagy by Bnip3 involves Drp1-mediated mitochondrial fission and recruitment of Parkin in cardiac myocytes.Am. J. Physiol. Heart Circ. Physiol.301H1924–H1931.
52
LeeY. S.JungY. Y.ParkM. H.YeoI. J.ImH. S.NamK. T.et al (2018). Deficiency of parkin suppresses melanoma tumor development and metastasis through inhibition of MFN2 ubiquitination.Cancer Lett.433156–164. 10.1016/j.canlet.2018.07.007
53
LewisM. R.LewisW. H. (1915). Mitochondria (and other cytoplasmic structures) in tissue cultures.Dev. Dyn.17339–401. 10.1002/aja.1000170304
54
LiC.ZhangY.ChengX.YuanH.ZhuS.LiuJ.et al (2018). PINK1 and PARK2 suppress pancreatic tumorigenesis through control of mitochondrial iron-mediated immunometabolism.Dev. Cell46441–455.e8.
55
LiF.XuY.XuX.GeS.ZhangF.ZhangH.et al (2020). lncRNA HotairM1 depletion promotes self-renewal of cancer stem cells through HOXA1-nanog regulation loop.Mol. Ther. Nucleic Acids22456–470. 10.1016/j.omtn.2020.09.008
56
LiJ.ZhaiD. S.HuangQ.ChenH. L.ZhangZ.TanQ. F. (2019). LncRNA DCST1-AS1 accelerates the proliferation, metastasis and autophagy of hepatocellular carcinoma cell by AKT/mTOR signaling pathways.Eur. Rev. Med. Pharmacol. Sci.236091–6104.
57
LiP.HeJ.YangZ.GeS.ZhangH.ZhongQ.et al (2020). ZNNT1 long noncoding RNA induces autophagy to inhibit tumorigenesis of uveal melanoma by regulating key autophagy gene expression.Autophagy161186–1199. 10.1080/15548627.2019.1659614
58
LiW.LiY.SirajS.JinH.FanY.YangX.et al (2019). FUN14 Domain-Containing 1-mediated mitophagy suppresses hepatocarcinogenesis by inhibition of inflammasome activation in mice.Hepatology69604–621. 10.1002/hep.30191
59
LiX.JinF.LiY. (2021). A novel autophagy-related lncRNA prognostic risk model for breast cancer.J. Cell. Mol. Med.254–14. 10.1111/jcmm.15980
60
LiX.ZhaoQ.QiJ.WangW.ZhangD.LiZ.et al (2018). lncRNA Ftx promotes aerobic glycolysis and tumor progression through the PPARgamma pathway in hepatocellular carcinoma.Int. J. Oncol.53551–566.
61
LiX.ZhouY.YangL.MaY.PengX.YangS.et al (2020). LncRNA NEAT1 promotes autophagy via regulating miR-204/ATG3 and enhanced cell resistance to sorafenib in hepatocellular carcinoma.J. Cell. Physiol.2353402–3413. 10.1002/jcp.29230
62
LiY.LiC.LiD.YangL.JinJ.ZhangB. (2019). lncRNA KCNQ1OT1 enhances the chemoresistance of oxaliplatin in colon cancer by targeting the miR-34a/ATG4B pathway.Oncotargets Ther.122649–2660. 10.2147/ott.s188054
63
LiaoM.LiaoW.XuN.LiB.LiuF.ZhangS.et al (2019). LncRNA EPB41L4A-AS1 regulates glycolysis and glutaminolysis by mediating nucleolar translocation of HDAC2.EBioMedicine41200–213. 10.1016/j.ebiom.2019.01.035
64
LiuC.PengZ.LiP.FuH.FengJ.ZhangY.et al (2020). lncRNA RMST Suppressed GBM Cell Mitophagy through Enhancing FUS SUMOylation.Mol. Ther. Nucleic Acids191198–1208. 10.1016/j.omtn.2020.01.008
65
LiuF.AiF. Y.ZhangD. C.TianL.YangZ. Y.LiuS. J. (2020). LncRNA NEAT1 knockdown attenuates autophagy to elevate 5-FU sensitivity in colorectal cancer via targeting miR-34a.Cancer Med.91079–1091. 10.1002/cam4.2746
66
LiuK.LeeJ.KimJ. Y.WangL.TianY.ChanS. T.et al (2017). Mitophagy controls the activities of tumor suppressor p53 to regulate hepatic cancer stem cells.Mol. Cell68:281. 10.1016/j.molcel.2017.09.022
67
LiuL.FengD.ChenG.ChenM.ZhengQ.SongP.et al (2012). Mitochondrial outer-membrane protein FUNDC1 mediates hypoxia-induced mitophagy in mammalian cells.Nat. Cell Biol.14177–185. 10.1038/ncb2422
68
LiuL.ZuoZ.LuS.WangL.LiuA.LiuX. (2018). Silencing of PINK1 represses cell growth, migration and induces apoptosis of lung cancer cells.Biomed. Pharmacother.106333–341. 10.1016/j.biopha.2018.06.128
69
LiuR.WangX.ShenY.HeA. (2021). Long non-coding RNA-based glycolysis-targeted cancer therapy: feasibility, progression and limitations.Mol. Biol. Rep.482713–2727. 10.1007/s11033-021-06247-7
70
LuanF.ChenW.ChenM.YanJ.ChenH.YuH.et al (2019). An autophagy-related long non-coding RNA signature for glioma.FEBS Open Bio9653–667.
71
Macher-GoeppingerS.KeithM.HatibogluG.HohenfellnerM.SchirmacherP.RothW.et al (2017). Expression and functional characterization of the BNIP3 protein in renal cell carcinomas.Transl. Oncol.10869–875. 10.1016/j.tranon.2017.08.008
72
MaesH.Van EygenS.KryskoD. V.VandenabeeleP.NysK.RillaertsK.et al (2014). BNIP3 supports melanoma cell migration and vasculogenic mimicry by orchestrating the actin cytoskeleton.Cell Death Dis.5:e1127. 10.1038/cddis.2014.94
73
MarinkovićM.ŠprungM.NovakI. (2021). Dimerization of mitophagy receptor BNIP3L/NIX is essential for recruitment of autophagic machinery.Autophagy171232–1243. 10.1080/15548627.2020.1755120
74
MatsudaN.SatoS.ShibaK.OkatsuK.SaishoK.GautierC. A.et al (2010). PINK1 stabilized by mitochondrial depolarization recruits Parkin to damaged mitochondria and activates latent Parkin for mitophagy.J. Cell Biol.189211–221. 10.1083/jcb.200910140
75
MaugeriG.D’AmicoA. G.MagroG.SalvatorelliL.BarbagalloG. M.SacconeS.et al (2015). Expression profile of parkin isoforms in human gliomas.Int. J. Oncol.471282–1292. 10.3892/ijo.2015.3105
76
McWilliamsT. G.PrescottA. R.AllenG. F.TamjarJ.MunsonM. J.ThomsonC.et al (2016). mito-QC illuminates mitophagy and mitochondrial architecture in vivo.J. Cell Biol.214333–345. 10.1083/jcb.201603039
77
MelloT.SimeoneI.GalliA. (2019). Mito-nuclear communication in hepatocellular carcinoma metabolic rewiring.Cells8:417. 10.3390/cells8050417
78
MissiroliS.PerroneM.GenoveseI.PintonP.GiorgiC. (2020). Cancer metabolism and mitochondria: finding novel mechanisms to fight tumours.EBioMedicine59:102943. 10.1016/j.ebiom.2020.102943
79
Montava-GarrigaL.GanleyI. G. (2020). Outstanding questions in mitophagy: What we do and do not know.J. Mol. Biol.432206–230. 10.1016/j.jmb.2019.06.032
80
MoralesP. E.Arias-DuránC.Ávalos-GuajardoY.AedoG.VerdejoH. E.ParraV.et al (2020). Emerging role of mitophagy in cardiovascular physiology and pathology.Mol. Aspects Med.71:100822. 10.1016/j.mam.2019.09.006
81
MurakawaT.YamaguchiO.HashimotoA.HikosoS.TakedaT.OkaT.et al (2015). Bcl-2-like protein 13 is a mammalian Atg32 homologue that mediates mitophagy and mitochondrial fragmentation.Nat. Commun.6:7527.
82
NaikP. P.BirbrairA.BhutiaS. K. (2019). Mitophagy-driven metabolic switch reprograms stem cell fate.Cell. Mol. Life Sci.7627–43. 10.1007/s00018-018-2922-9
83
NaikP. P.MukhopadhyayS.PandaP. K.SinhaN.DasC. K.MishraR.et al (2018). Autophagy regulates cisplatin-induced stemness and chemoresistance via the upregulation of CD44, ABCB1 and ADAM17 in oral squamous cell carcinoma.Cell Prolif.51:e12411.
84
NarendraD.TanakaA.SuenD. F.YouleR. J. (2008). Parkin is recruited selectively to impaired mitochondria and promotes their autophagy.J. Cell Biol.183795–803. 10.1083/jcb.200809125
85
NohJ. H.KimK. M.AbdelmohsenK.YoonJ. H.PandaA. C.MunkR.et al (2016). HuR and GRSF1 modulate the nuclear export and mitochondrial localization of the lncRNA RMRP.Genes Dev.301224–1239.
86
OhshimaK.MoriiE. (2021). Metabolic reprogramming of cancer cells during tumor progression and metastasis.Metabolites11:28. 10.3390/metabo11010028
87
OkamotoK.Kondo-OkamotoN.OhsumiY. (2009). Mitochondria-anchored receptor Atg32 mediates degradation of mitochondria via selective autophagy.Dev. Cell1787–97. 10.1016/j.devcel.2009.06.013
88
OnishiM.OkamotoK. (2021). Mitochondrial clearance: mechanisms and roles in cellular fitness.FEBS Lett.5951239–1263. 10.1002/1873-3468.14060
89
PalikarasK.DaskalakiI.MarkakiM.TavernarakisN. (2017). Mitophagy and age-related pathologies: development of new therapeutics by targeting mitochondrial turnover.Pharmacol. Ther.178157–174. 10.1016/j.pharmthera.2017.04.005
90
PalikarasK.LionakiE.TavernarakisN. (2018). Mechanisms of mitophagy in cellular homeostasis, physiology and pathology.Nat. Cell Biol.201013–1022. 10.1038/s41556-018-0176-2
91
PangB. P. S.ChanW. S.ChanC. B. (2021). Mitochondria Homeostasis and Oxidant/Antioxidant Balance in Skeletal Muscle-Do Myokines Play a Role?Antioxidants10:179. 10.3390/antiox10020179
92
PavlovaN. N.ThompsonC. B. (2016). The emerging hallmarks of cancer metabolism.Cell Metab.2327–47. 10.1016/j.cmet.2015.12.006
93
PicklesS.VigiéP.YouleR. J. (2018). Mitophagy and quality control mechanisms in mitochondrial maintenance.Curr. Biol.28R170–R185.
94
PooleL. P.MacleodK. F. (2021). Mitophagy in tumorigenesis and metastasis.Cell. Mol. Life Sci.783817–3851. 10.1007/s00018-021-03774-1
95
PoulogiannisG.McIntyreR. E.DimitriadiM.AppsJ. R.WilsonC. H.IchimuraK.et al (2010). PARK2 deletions occur frequently in sporadic colorectal cancer and accelerate adenoma development in Apc mutant mice.Proc. Natl. Acad. Sci. U.S.A.10715145–15150. 10.1073/pnas.1009941107
96
QuirosP. M.MottisA.AuwerxJ. (2016). Mitonuclear communication in homeostasis and stress.Nat. Rev. Mol. Cell Biol.17213–226. 10.1038/nrm.2016.23
97
RackhamO.ShearwoodA. M.MercerT. R.DaviesS. M.MattickJ. S.FilipovskaA. (2011). Long noncoding RNAs are generated from the mitochondrial genome and regulated by nuclear-encoded proteins.RNA172085–2093. 10.1261/rna.029405.111
98
RichardV. R.LeonovA.BeachA.BursteinM. T.KoupakiO.Gomez-PerezA.et al (2013). Macromitophagy is a longevity assurance process that in chronologically aging yeast limited in calorie supply sustains functional mitochondria and maintains cellular lipid homeostasis.Aging5234–269. 10.18632/aging.100547
99
Roca-PortolesA.TaitS. W. G. (2021). Mitochondrial quality control: from molecule to organelle.Cell. Mol. Life Sci.783853–3866. 10.1007/s00018-021-03775-0
100
RogovV. V.SuzukiH.MarinkovićM.LangV.KatoR.KawasakiM.et al (2017). Phosphorylation of the mitochondrial autophagy receptor Nix enhances its interaction with LC3 proteins.Sci. Rep.7:1131.
101
RojanskyR.ChaM. Y.ChanD. C. (2016). Elimination of paternal mitochondria in mouse embryos occurs through autophagic degradation dependent on PARKIN and MUL1.eLife5:e17896.
102
SandovalH.ThiagarajanP.DasguptaS. K.SchumacherA.PrchalJ. T.ChenM.et al (2008). Essential role for Nix in autophagic maturation of erythroid cells.Nature454232–235. 10.1038/nature07006
103
SchweersR. L.ZhangJ.RandallM. S.LoydM. R.LiW.DorseyF. C.et al (2007). NIX is required for programmed mitochondrial clearance during reticulocyte maturation.Proc. Natl. Acad. Sci. U.S.A.10419500–19505. 10.1073/pnas.0708818104
104
ScottS. V.KlionskyD. J. (1998). Delivery of proteins and organelles to the vacuole from the cytoplasm.Curr. Opin. Cell Biol.10523–529. 10.1016/s0955-0674(98)80068-9
105
SessionsD. T.KashatusD. F. (2021). Mitochondrial dynamics in cancer stem cells.Cell. Mol. Life Sci.783803–3816.
106
ShankaraiahR. C.VeroneseA.SabbioniS.NegriniM. (2018). Non-coding RNAs in the reprogramming of glucose metabolism in cancer.Cancer Lett.419167–174. 10.1016/j.canlet.2018.01.048
107
ShengJ. Q.WangM. R.FangD.LiuL.HuangW. J.TianD. A.et al (2021). LncRNA NBR2 inhibits tumorigenesis by regulating autophagy in hepatocellular carcinoma.Biomed. Pharmacother.133:111023. 10.1016/j.biopha.2020.111023
108
SinJ.AndresA. M.TaylorD. J.WestonT.HiraumiY.StotlandA.et al (2016). Mitophagy is required for mitochondrial biogenesis and myogenic differentiation of C2C12 myoblasts.Autophagy12369–380. 10.1080/15548627.2015.1115172
109
SinghB.Modica-NapolitanoJ. S.SinghK. K. (2017). Defining the momiome: promiscuous information transfer by mobile mitochondria and the mitochondrial genome.Semin. Cancer Biol.471–17. 10.1016/j.semcancer.2017.05.004
110
SliterD. A.MartinezJ.HaoL.ChenX.SunN.FischerT. D.et al (2018). Parkin and PINK1 mitigate STING-induced inflammation.Nature561258–262. 10.1038/s41586-018-0448-9
111
SongW. H.YiY. J.SutovskyM.MeyersS.SutovskyP. (2016). Autophagy and ubiquitin-proteasome system contribute to sperm mitophagy after mammalian fertilization.Proc. Natl. Acad. Sci. U.S.A.113E5261–E5270.
112
SpinelliJ. B.HaigisM. C. (2018). The multifaceted contributions of mitochondria to cellular metabolism.Nat. Cell Biol.20745–754. 10.1038/s41556-018-0124-1
113
SpringerM. Z.PooleL. P.DrakeL. E.Bock-HughesA.BolandM. L.SmithA. G.et al (2021). BNIP3-dependent mitophagy promotes cytosolic localization of LC3B and metabolic homeostasis in the liver.Autophagy.10.1080/15548627.2021.1877469[Epub ahead of print].
114
SunJ. L.HeX. S.YuY. H.ChenZ. C. (2004). [Expression and structure of BNIP3L in lung cancer].Chin. J. Cancer238–14.
115
SunM. Y.ZhuJ. Y.ZhangC. Y.ZhangM.SongY. N.RahmanK.et al (2017). Autophagy regulated by lncRNA HOTAIR contributes to the cisplatin-induced resistance in endometrial cancer cells.Biotechnol. Lett.391477–1484. 10.1007/s10529-017-2392-4
116
TayS. P.YeoC. W.ChaiC.ChuaP. J.TanH. M.AngA. X.et al (2010). Parkin enhances the expression of cyclin-dependent kinase 6 and negatively regulates the proliferation of breast cancer cells.J. Biol. Chem.28529231–29238. 10.1074/jbc.m110.108241
117
TranM.ReddyP. H. (2020). Defective autophagy and mitophagy in aging and Alzheimer’s Disease.Front. Neurosci.14:612757. 10.3389/fnins.2020.612757
118
TrempeJ. F.SauvéV.GrenierK.SeirafiM.TangM. Y.MénadeM.et al (2013). Structure of parkin reveals mechanisms for ubiquitin ligase activation.Science3401451–1455. 10.1126/science.1237908
119
TsukadaM.OhsumiY. (1993). Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae.FEBS Lett.333169–174. 10.1016/0014-5793(93)80398-e
120
UmJ. H.YunJ. (2017). Emerging role of mitophagy in human diseases and physiology.BMB Rep.50299–307. 10.5483/bmbrep.2017.50.6.056
121
Vara-PerezM.Felipe-AbrioB.AgostinisP. (2019). Mitophagy in cancer: a tale of adaptation.Cells8:493. 10.3390/cells8050493
122
Vazquez-MartinA.Van den HauteC.CufíS.Corominas-FajaB.CuyàsE.Lopez-BonetE.et al (2016). Mitophagy-driven mitochondrial rejuvenation regulates stem cell fate.Aging81330–1352. 10.18632/aging.100976
123
VillaE.ProïcsE.Rubio-PatiñoC.ObbaS.ZuninoB.BossowskiJ. P.et al (2017). Parkin-independent mitophagy controls chemotherapeutic response in cancer cells.Cell Rep.202846–2859. 10.1016/j.celrep.2017.08.087
124
WaiT.LangerT. (2016). Mitochondrial dynamics and metabolic regulation.Trends Endocrinol. Metab.27105–117. 10.1016/j.tem.2015.12.001
125
WangC. Z.YanG. X.DongD. S.XinH.LiuZ. Y. (2019). LncRNA-ATB promotes autophagy by activating Yes-associated protein and inducing autophagy-related protein 5 expression in hepatocellular carcinoma.World J. Gastroenterol.255310–5322. 10.3748/wjg.v25.i35.5310
126
WangJ.XieS.YangJ.XiongH.JiaY.ZhouY.et al (2019). The long noncoding RNA H19 promotes tamoxifen resistance in breast cancer via autophagy.J. Hematol. Oncol.12:81.
127
WangS. Y.WangX.ZhangC. Y. (2020). LncRNA SNHG7 enhances chemoresistance in neuroblastoma through cisplatin-induced autophagy by regulating miR-329-3p/MYO10 axis.Eur. Rev. Med. Pharmacol. Sci.243805–3817.
128
WangX.ChengM. L.GongY.MaW. J.LiB.JiangY. Z. (2020). LncRNA DANCR promotes ATG7 expression to accelerate hepatocellular carcinoma cell proliferation and autophagy by sponging miR-222-3p.Eur. Rev. Med. Pharmacol. Sci.248778–8787.
129
WangX. L.FengS. T.WangZ. Z.ChenN. H.ZhangY. (2021). Role of mitophagy in mitochondrial quality control: mechanisms and potential implications for neurodegenerative diseases.Pharmacol. Res.165:105433. 10.1016/j.phrs.2021.105433
130
WeiY.ChiangW. C.SumpterR.Jr.MishraP.LevineB. (2017). Prohibitin 2 is an inner mitochondrial membrane mitophagy receptor.Cell168224–238.e10.
131
WhelanK. A.ChandramouleeswaranP. M.TanakaK.NatsuizakaM.GuhaM.SrinivasanS.et al (2017). Autophagy supports generation of cells with high CD44 expression via modulation of oxidative stress and Parkin-mediated mitochondrial clearance.Oncogene364843–4858. 10.1038/onc.2017.102
132
WuJ.ZhuP.LuT.DuY.WangY.HeL.et al (2019). The long non-coding RNA LncHDAC2 drives the self-renewal of liver cancer stem cells via activation of Hedgehog signaling.J. Hepatol.70918–929. 10.1016/j.jhep.2018.12.015
133
WuL.ZhangD.ZhouL.PeiY.ZhuangY.CuiW.et al (2019). FUN14 domain-containing 1 promotes breast cancer proliferation and migration by activating calcium-NFATC1-BMI1 axis.EBioMedicine41384–394. 10.1016/j.ebiom.2019.02.032
134
WuW.TianW.HuZ.ChenG.HuangL.LiW.et al (2014). ULK1 translocates to mitochondria and phosphorylates FUNDC1 to regulate mitophagy.EMBO Rep.15566–575. 10.1002/embr.201438501
135
XiangG.YangL.LongQ.ChenK.TangH.WuY.et al (2017). BNIP3L-dependent mitophagy accounts for mitochondrial clearance during 3 factors-induced somatic cell reprogramming.Autophagy131543–1555. 10.1080/15548627.2017.1338545
136
XiangX.FuY.ZhaoK.MiaoR.ZhangX.MaX.et al (2021). Cellular senescence in hepatocellular carcinoma induced by a long non-coding RNA-encoded peptide PINT87aa by blocking FOXM1-mediated PHB2.Theranostics114929–4944. 10.7150/thno.55672
137
XiaoZ. D.HanL.LeeH.ZhuangL.ZhangY.BaddourJ.et al (2017). Energy stress-induced lncRNA FILNC1 represses c-Myc-mediated energy metabolism and inhibits renal tumor development.Nat. Commun.8:783.
138
XinL.ZhouQ.YuanY. W.ZhouL. Q.LiuL.LiS. H.et al (2019). METase/lncRNA HULC/FoxM1 reduced cisplatin resistance in gastric cancer by suppressing autophagy.J. Cancer Res. Clin. Oncol.1452507–2517. 10.1007/s00432-019-03015-w
139
XiongH.NiZ.HeJ.JiangS.LiX.HeJ.et al (2017). LncRNA HULC triggers autophagy via stabilizing Sirt1 and attenuates the chemosensitivity of HCC cells.Oncogene363528–3540. 10.1038/onc.2016.521
140
YamanoK.YouleR. J. (2013). PINK1 is degraded through the N-end rule pathway.Autophagy91758–1769. 10.4161/auto.24633
141
YamashitaK.MiyataH.MakinoT.MasuikeY.FurukawaH.TanakaK.et al (2017). High expression of the mitophagy-related protein pink1 is associated with a poor response to chemotherapy and a poor prognosis for patients treated with Neoadjuvant Chemotherapy for Esophageal Squamous Cell Carcinoma.Ann. Surg. Oncol.244025–4032. 10.1245/s10434-017-6096-8
142
YanC.LuoL.GuoC. Y.GotoS.UrataY.ShaoJ. H.et al (2017). Doxorubicin-induced mitophagy contributes to drug resistance in cancer stem cells from HCT8 human colorectal cancer cells.Cancer Lett.38834–42. 10.1016/j.canlet.2016.11.018
143
YangF.ZhangH.MeiY.WuM. (2014). Reciprocal regulation of HIF-1alpha and lincRNA-p21 modulates the Warburg effect.Mol. Cell5388–100. 10.1016/j.molcel.2013.11.004
144
ZaarourR. F.AzakirB.HajamE. Y.NawaflehH.ZeinelabdinN. A.EngelsenA. S. T.et al (2021). Role of hypoxia-mediated autophagy in tumor cell death and survival.Cancers13:533. 10.3390/cancers13030533
145
ZhangC.LinM.WuR.WangX.YangB.LevineA. J.et al (2011). Parkin, a p53 target gene, mediates the role of p53 in glucose metabolism and the Warburg effect.Proc. Natl. Acad. Sci. U.S.A.10816259–16264. 10.1073/pnas.1113884108
146
ZhangT.XueL.LiL.TangC.WanZ.WangR.et al (2016). BNIP3 protein suppresses PINK1 kinase proteolytic cleavage to promote mitophagy.J. Biol. Chem.29121616–21629. 10.1074/jbc.m116.733410
147
ZhangW.RenH.XuC.ZhuC.WuH.LiuD.et al (2016). Hypoxic mitophagy regulates mitochondrial quality and platelet activation and determines severity of I/R heart injury.eLife5:e21407.
148
ZhangW.SirajS.ZhangR.ChenQ. (2017). Mitophagy receptor FUNDC1 regulates mitochondrial homeostasis and protects the heart from I/R injury.Autophagy131080–1081. 10.1080/15548627.2017.1300224
149
ZhaoY.LiuS.LiX.ZhouL.MengY.LiY.et al (2019). Aberrant shuttling of long noncoding RNAs during the mitochondria-nuclear crosstalk in hepatocellular carcinoma cells.Am. J. Cancer Res.9999–1008.
150
ZhaoY.SunL.WangR. R.HuJ. F.CuiJ. (2018). The effects of mitochondria-associated long noncoding RNAs in cancer mitochondria: new players in an old arena.Crit. Rev. Oncol.13176–82. 10.1016/j.critrevonc.2018.08.005
151
ZhaoY.ZhouL.LiH.SunT.WenX.LiX.et al (2021). Nuclear-Encoded lncRNA MALAT1 epigenetically controls metabolic reprogramming in hcc cells through the mitophagy pathway.Mol. Ther. Nucleic Acids23264–276. 10.1016/j.omtn.2020.09.040
152
ZhengR.YaoQ.XieG.DuS.RenC.WangY.et al (2015). TAT-ODD-p53 enhances the radiosensitivity of hypoxic breast cancer cells by inhibiting Parkin-mediated mitophagy.Oncotarget617417–17429. 10.18632/oncotarget.4002
153
ZiaA.FarkhondehT.Pourbagher-ShahriA. M.SamarghandianS. (2021). The roles of mitochondrial dysfunction and reactive oxygen species in aging and senescence.Curr. Mol. Med.10.2174/1566524021666210218112616[Epub ahead of print].
Summary
Keywords
mitochondria, mitophagy, cancer metabolism, long non-coding RNA, cancer stem cells, cancer therapy
Citation
Li Y, Li W, Hoffman AR, Cui J and Hu J-F (2021) The Nucleus/Mitochondria-Shuttling LncRNAs Function as New Epigenetic Regulators of Mitophagy in Cancer. Front. Cell Dev. Biol. 9:699621. doi: 10.3389/fcell.2021.699621
Received
23 April 2021
Accepted
20 August 2021
Published
08 September 2021
Volume
9 - 2021
Edited by
Simão Teixeira da Rocha, University of Lisbon, Portugal
Reviewed by
Qi Liao, Ningbo University, China; Ping Hu, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (CAS), China
Updates
Copyright
© 2021 Li, Li, Hoffman, Cui and Hu.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Ji-Fan Hu, jifan@stanford.edu; hujifan@jlu.edu.cnJiuwei Cui, cuijw@jlu.edu.cn
This article was submitted to Developmental Epigenetics, a section of the journal Frontiers in Cell and Developmental Biology
Disclaimer
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.