In the original article, there was an error. The restriction enzymes that are to be used when cloning target genes of interest into the Ed-modified pMT/V5-His plasmid were incorrectly described as “5'-BamHI and 3'-XhoI” instead of “5'-BglII and 3'-SalI.”
A correction has been made to the legend and image of Figure 1B as published and the corrected Figure 1 appears below.
Figure 1
A correction has been made to Stepwise Procedures, Molecular Cloning of Ed Fusion Constructs:
“Expression of Ed fusion constructs in S2 cells is achieved using the copper inducible metallothionein promotor within the pMT expression vector (Thermo Fisher). Cloning and construction of pMT:Ed plasmids has been previously detailed (Johnston et al., 2009). We have generated plasmids that yield either GFP- or FLAG-tagged versions of the Ed fusion, with the general structure of Ed:GFP-X, where X represents the desired cloned gene of interest (Figure 1). Both plasmids are linearized using 5′-BglII and 3′-SalI restriction digest, which can be ligated with identically digested inserts or those digested with isocaudameric enzymes such as 5′-BamHI and 3′-XhoI. Cloning should be done using standard molecular techniques and verified using Sanger sequencing methods.”
The author apologizes for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
References
1
JohnstonC. A.HironoK.PrehodaK. E.DoeC. Q. (2009). Identification of an Aurora-A/PinsLINKER/Dlg spindle orientation pathway using induced cell polarity in S2 cells. Cell138, 1150–1163. 10.1016/j.cell.2009.07.041
Summary
Keywords
cell polarity, spindle orientation, mitosis, reconstitution, neuroblast
Citation
Johnston CA (2021) Corrigendum: A Cell Adhesion-Based Reconstitution Method for Studying Cell Polarity. Front. Cell Dev. Biol. 9:705599. doi: 10.3389/fcell.2021.705599
Received
05 May 2021
Accepted
24 May 2021
Published
22 June 2021
Volume
9 - 2021
Edited and reviewed by
Zhiyi Lv, Ocean University of China, China
Updates
Copyright
© 2021 Johnston.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Christopher A. Johnston johnstca@unm.edu
This article was submitted to Signaling, a section of the journal Frontiers in Cell and Developmental Biology
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