In the original article, there was a mistake in Figure 5A as published. In the original Figure 5A, there was only sequence of one gRNA, but the sequences of two gRNAs should be provided in the vector map for knocking out miR-185-5p, thus we have updated the sequences of two gRNAs in the corrected Figure 5A. The corrected Figure 5A appears below.
FIGURE 5
(A) The vector map for miR-185-5p vector builder. U6 promoter: Human U6 promoter. {gRNA1: 20nt_TGGGAGAGGTATATCCGGAA; gRNA2: 20nt_GCTGCCTGGAGACACATCGA}: the component entered by user. gRNA scaffold, Chimeric gRNA scaffold; Terminator, U6 terminator; CBh promoter, chicken beta act n hybrid promoter; 3xFLAG, three tandem flag epitopes; SV40 NLS, SV40 nuclear localization signal; hCas9, human codon-optimized Cas9; Nucleoplasmin NLS, nucleoplasmin nuclear localization signal; BGH pA, bovine growth hormone polyadenylation; Ampicillin, ampicillin resistance gene; pUCori, pUC origin of replication. (B) Electrophoretic band chart for genotype detection. Green arrows represent WT rats, yellow represent KO, and blue arrow represents HET. The markers exhibit 100, 250, 500, 750, 1,000, and 2,000 bp, respectively. (C) The relative expression of Igfbp3 in the rats of 185-WT and 185-KO. The relative expression was relative to that of the 185-WT in cortex group. (D) The glucose-uptake images in the brain and SUV max detected by PET-CT 2-month post HIE. (E,H) TTC staining and quantitative analysis of infarct ratio, respectively, in the rats of 185-WT and 185-KO. Scale bar = 1 cm. Pale color represents infarct area, n = 6/group. (F,I) HE staining and the cell size analysis of cortex and hippocampus, respectively, in the rats of 185-WT and 185-KO. Scale bar = 50 μm, n = 6/group. (G) Nissl staining in cortex and hippocampus between 185-WT and 185-KO groups. The white arrow represents surviving neurons. The red arrow represents dark neurons. Scale bar = 100 μm. (J,K) Quantitative histogram for total neuron and dark neuron in cortex and hippo in these groups, n = 6/group. (L,M) The time spent rearing and grooming in the open field test 1 month after HIE, respectively, n = 6/group. (N) NSS score at 1 month after HIE. (O) The duration of on the rotarod bar 1 month after HIE, n = 6/group. (P) The latency to target for the first 5 days training in the MWM test, n = 6/group. (Q) Target crossings in MWM test in the 6th day of testing. (R) The time spent in the initial, wrong and food arms of Y-maze at 1-month post HIE, n = 6/group. HET, heterozygote; 185-WT, miR-185-5p wild type; 185-KO, miR-185-5p knockout; PET-CT, positron emission tomography-computed tomography; SUV, standardized uptake value; HE, hematoxylin-eosin; Hippo, hippocampus. All data are presented as mean ± SD, *p < 0.05.
In the original article, there was a mistake in the legend for Figure 5 as published. The legend of original Figure 5A has been updated owing to the correction made in Figure 5A (details stated in the above section). The correct legend for Figure 5A appears below.
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
Statements
Publisher’s note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Summary
Keywords
hypoxic ischemic encephalopathy, Vi4, miRNA-185-5p, IGFBP3, neuron survival, cell apoptosis
Citation
Xiong L-L, Xue L-L, Du R-L, Zhou H-L, Tan Y-X, Ma Z, Jin Y, Zhang Z-B, Xu Y, Hu Q, Bobrovskaya L, Zhou X-F, Liu J and Wang T-H (2022) Corrigendum: Vi4-miR-185-5p-Igfbp3 Network Protects the Brain From Neonatal Hypoxic Ischemic Injury via Promoting Neuron Survival and Suppressing the Cell Apoptosis. Front. Cell Dev. Biol. 10:852539. doi: 10.3389/fcell.2022.852539
Received
11 January 2022
Accepted
24 January 2022
Published
16 February 2022
Volume
10 - 2022
Edited and reviewed by
Nu Zhang, The University of Texas Health Science Center at San Antonio, United States
Updates
Copyright
© 2022 Xiong, Xue, Du, Zhou, Tan, Ma, Jin, Zhang, Xu, Hu, Bobrovskaya, Zhou, Liu and Wang.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Ting-Hua Wang, Wangth_email@163.com; Jia Liu, liujiaaixuexi@126.com; Liu-Lin Xiong, 499465010@qq.com
†These authors have contributed equally to this work
This article was submitted to Cell Death and Survival, a section of the journal Frontiers in Cell and Developmental Biology
Disclaimer
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.