In the published article, there is an error in Figure 3 and Figure 4 as published. The two figures are inverted, while the captions are correct. The corrected Figure 3 and Figure 4 and its caption appear below.
FIGURE 3
Chromosome 15q13.3 and hiPSCs derived from individuals with CNV. Graphic representation of chromosomal region 15q13.3 showing BreakPoint regions BP3, BP4 and BP5 and the extensions the microdeletions and microduplications present in the hiPSCs in the published studies.
FIGURE 4
An insight into molecular effects of CNV 15q13.3. Cells carrying CNV duplications show decreased calcium flux associated with the α7 receptor, downregulation of JAK2-PI3K pathway, decreased assembly and trafficking of nAchRs, and ER stress. Cells carrying CNV deletions exhibit decreased α7nAchRs calcium flux and downregulation of JAK2-PI3K pathway.
In the published article, there is an error in Table 1 as published. The table is not paginated correctly.Table 1 and its caption appear below.
TABLE 1
| Authors | Cell type of origin | Type of mutation | Gene expression analysis | Calcium assays | Pharmacological characterization | ER stress | Aβ1-42 uptake | Interneuron migration | DNA analysis |
|---|---|---|---|---|---|---|---|---|---|
| Gill et al., 2013 | Fibroblasts | — | — | Whole-cell patch-clamp recordings, fluorescence-based calcium imaging | With TQS, 4BP-TQs, and MLA | — | — | — | — |
| Chatzidaki et al., 2015 | Fibroblasts | — | CHRNA7 and CHRFAM7A | FLIPR-based assay | With Type II PAM (PNU-120596) and MLA | — | — | — | — |
| Calcium imaging, Patchclamp recording | |||||||||
| Gillentine et al., 2017 | Fibroblasts | CHRNA7 deletions and duplications | CHRNA7 (higher in duplications and lower in deletions) | FLIPR-based assay | With Type II PAM (PNU-120596) and MLA | Increased in duplicated lines | — | — | — |
| Turco et al., 2018 | Fibroblasts | Single gene duplication (CHRNA7) | — | — | — | — | — | — | — |
| Larsen et al., 2019 | Fibroblasts | Yes, but not available | CHRNA7 and CHRFAM7A | Calcium imaging | With Type-II PAM (PNU-120596) and Type-I/II (JNJ-39393406, AF58801) | — | — | — | — |
| Ihnatovych et al., 2019 | Fibroblasts | CHRFAM7A null, CHRFAM7A 1 copy | CHRNA7 and CHRFAM7A (which increases during differentiation in 1-copy line) | Single cell-attached and whole-cell patch-clamp recording (reduced activity in 1-copy line) | With Type-II PAM (PNU 120596) (faster desensitization in 1-copy line) | — | Fluorescence imaging and ELISA assay (decreased in 1-copy line) | — | — |
| Szigeti et al., 2020 | Fibroblasts | CHRFAM7A null, CHRFAM7A 1 copy, Transfected CHRFAM7A | CHRFAM7A | Single cell-attached and whole-cell patch-clamp recording | — | — | Fluorescence imaging and ELISA assay (decreased in 1-copy and transfected lines) | — | — |
| Ihnatovych et al., 2020 | Fibroblasts | CHRFAM7A null, CHRFAM7A 1 copy Transfected CHRFAM7A | CHRNA7 and CHRFAM7A | — | — | — | Fluorescence imaging and ELISA assay(decreased in 1-copy and transfected lines) | — | — |
| Meganathan et al., 2021 | Renal epithelial cells | Single gene duplication (CHRNA7) | CHRNA7 (increased in duplicated lines) | Whole-cell voltage and current-clamp recording (increased choline responsiveness and decreased Ach one in duplicated lines) | — | Increased in the affected proband | — | Organoid-based neuronal migration assay (diminished in the affected proband) | — |
| Zhang et al., 2021 | Fibroblasts | CHRNA7 deletions | — | — | — | — | — | — | Methyl-Seq and ATAC-Seq analysis |
Summary of the studies based on the hiPSCs model for studying 15q13.3 CNV.
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
Statements
Publisher’s note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Summary
Keywords
neurodevelopmental disorders, neuropsychiatric disorders, 15q11-13, CHRNA7, nicotinic acetylcholine receptor, copy number variation, CNV
Citation
Giovenale AMG, Ruotolo G, Soriano AA, Turco EM, Rotundo G, Casamassa A, D’Anzi A, Vescovi AL and Rosati J (2023) Corrigendum: Deepening the understanding of CNVs on chromosome 15q11–13 by using hiPSCs: An overview. Front. Cell Dev. Biol. 11:1141334. doi: 10.3389/fcell.2023.1141334
Received
10 January 2023
Accepted
16 January 2023
Published
01 February 2023
Volume
11 - 2023
Edited and reviewed by
Mirella Dottori, University of Wollongong, Australia
Updates
Copyright
© 2023 Giovenale, Ruotolo, Soriano, Turco, Rotundo, Casamassa, D’Anzi, Vescovi and Rosati.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Jessica Rosati, j.rosati@css-mendel.it; Angelo Luigi Vescovi, angelo.vescovi@unimib.it
†These authors have contributed equally to this work
This article was submitted to Stem Cell Research, a section of the journal Frontiers in Cell and Developmental Biology
Disclaimer
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.