The mouse shares many morphological and genetic similarities with humans, making a valuable experimental model for studying human disease. Molecular tools to modify the mouse genome have been developed and refined over decades and with the advent of the CRISPR-Cas9 and similar technologies, targeted manipulations have become standard. In parallel, a wide spectrum of sophisticated morphological and functional imaging methods emerged recently, that allow precise two-dimensional (2D), three-dimensional (3D) and even four-dimensional (4D) visualisation of organs and tissues, often combined with the molecular and structural environments of cells and the intercellular space. Together, this enables examining the complex regulation of processes in ontogenesis and the mechanisms of tissue remodelling in mammals. This, in turn, is essential for understanding the principles of morphogenetic and physiological processes in humans and researching the causality of hereditary diseases.
In this Research Topic we aimed to collect Original Articles and Reviews, that introduce the reader to state-of-the-art imaging methods for studying the phenotype of normal and genetically engineered mouse embryos.
The first article introduces micro computed tomography (µCT). In a review paper, Handschuh and Glösmann offer excellent insights into the art of sample fixation, sample mounting, tissue contrasting and image analysis. They also put the theoretical consideration in praxis and provide impressive examples of qualitative and quantitative analysis of mouse embryo morphology.
In the following article, Scully and Larina switch to optical coherence tomography (OCT). They demonstrate the capability of this non-invasive technique for classical structural embryo phenotyping and pay special attention to the potential of OCT to study dynamic events by live phenotyping of cultured early mouse embryos. Technical limitations as well as future perspectives and the potential to become integrated in multimodal imaging approaches are comprehensively discussed.
Next, Anderson and Bamforth show results of using two cutting edge imaging techniques, “High resolution episcopic microscopy” (HREM) and µCT for gaining insights into basic morphogenetic events. Their article “Morphogenesis of the Mammalian Aortic Arch Arteries” visualizes and examines remodeling of the embryonic pharyngeal arch arteries in mice and men. It adds new details to the knowledge about the formation of the external carotid and subclavian artery, and to the role Tbx1 plays in the genesis of cardiovascular defects.
A contribution by Garcia-Canadilla et al. follows, where they apply structure tensor analysis on HREM-derived volume-data for characterising and for quantifying the myocardial architecture of mouse embryos at embryonic days (E)14.5 to E18.5. The quantitative results demonstrate gradual increase of the complexity of myocardial organization with developmental progress and a regional heterogeneity in myocardial architecture.
HREM data are also used in the article of Reissig et al. The authors carefully analysed cranial nerve topology in volume data of 152 C57BL/6 wild type mouse embryos produced in the “Deciphering the mechanisms of developmental disorders” (DMDD) program. The information was then used as a reference for diagnosing cranial nerve abnormalities in 4 knock-out lines produced in the DMDD project and for defining the range of norm variants in wild types.
An article of Petrelli et al. follows, in which the authors rely on an interesting mixture of imaging modalities for characterising a new mouse model created to study the mechanisms underlying Foetal Alcohol Syndrome Disorder (FASD). µCT, scanning electron microscopy, whole-mount in situ hybridization, and immunohistochemistry were applied to create data that allowed the detection of a number of craniofacial malformations recapitulating defects characteristic of prenatal alcohol exposure.
Finally, the review by Copp et al. demonstrates the merits of performing phenotyping of cultured embryos with the aid of stereomicroscopy. Besides providing imaging tips, they direct the reader to an innovative and largely unknown whole mouse embryo culture approach, which enables experimentation with mammalian embryos.
We like to express our gratefulness to all the imaging specialists who contributed to this Research Topic and hope that the embryo phenotyping community will profit from this Research Topic of highly interesting articles.
Statements
Author contributions
SG: Writing–original draft. DS: Writing–review and editing. GM: Writing–review and editing. WW: Writing–original draft.
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Summary
Keywords
mouse embryo, phenotyping, imaging, mouse model, Morphogenesis
Citation
Geyer SH, Szumska D, Martins GG and Weninger WJ (2023) Editorial: Phenotyping mouse embryos. Front. Cell Dev. Biol. 11:1284433. doi: 10.3389/fcell.2023.1284433
Received
28 August 2023
Accepted
30 August 2023
Published
05 September 2023
Volume
11 - 2023
Edited and reviewed by
Andrew B. Goryachev, University of Edinburgh, United Kingdom
Updates
Copyright
© 2023 Geyer, Szumska, Martins and Weninger.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Stefan H. Geyer, stefan.geyer@meduniwien.ac.at
Disclaimer
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.