Frontiers journals are at the top of citation and impact metrics

Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Energy Res. | doi: 10.3389/fenrg.2018.00098

Community assembly in wastewater-fed pilot-scale microbial electrolysis cells

 Sarah E. Cotterill1*,  Jan Dolfing1,  Thomas P. Curtis1 and Elizabeth S. Heidrich1
  • 1Newcastle University, United Kingdom

The formation of an electrochemically active biofilm is critical to the function of a Microbial Electrolysis Cell (MEC). We used Illumina 16S rDNA sequencing to analyse the formation and composition of anodic biofilms of two pilot-scale MECs, operated in continuous flow mode on domestic wastewater for over six months, and inoculated with that same wastewater. We observe: (i) a clear correlation between the frequency of detection of taxa in the MECs and their abundance in the metacommunity, (ii) the existence of a “core community” that was present across sites and (iii) the percentage of Geobacter tended to increase with longevity of retention time of the wastewater in the reactor. This suggests that: (i) community composition was largely governed by stochastic processes, (ii) that the technology should work on most if not all domestic wastewaters, as long as the anodes are seeded with the target wastewater and (iii) that deterministic factors may also play a role in establishing the anodic community. Geobacter, the archetypical electrogen in bioelectrochemical systems, comprised only 1.0 ± 0.7 % of the sequences recovered from a functioning pilot-scale MEC anode. Our results imply that influent flow rate may need to be optimised separately for start-up and for operating conditions for maximal performance.

Keywords: community assembly, rDNA sequencing, Microbial electrolysis cell (MEC), neutral community model, wastewater

Received: 28 Jun 2018; Accepted: 04 Sep 2018.

Edited by:

Vânia Oliveira, Faculdade de Engenharia, Universidade do Porto, Portugal

Reviewed by:

Hiroshige Matsumoto, Kyushu University, Japan
DEEPAK PANT, Flemish Institute for Technological Research, Belgium  

Copyright: © 2018 Cotterill, Dolfing, Curtis and Heidrich. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Sarah E. Cotterill, Newcastle University, Newcastle upon Tyne, NE1 7RU, North East England, United Kingdom, sarah.cotterill3@gmail.com