ORIGINAL RESEARCH article
Sec. Bioenergy and Biofuels
Sono-Assisted Alkali and Dilute Acid Pretreatment of Phragmites karka (Tall Reed Grass) to Enhance Enzymatic Digestibility for Bioethanol Conversion
- 1Institute of Bioresources and Sustainable Development (IBSD), A National Institute under Department of Biotechnology, Govt of India Takyelpat, Imphal, India
- 2Department of Botany, University of Delhi, Delhi, India
- 3Microbial processes and Technology Division, Centre for Biofuels National Institute for Interdisciplinary Science and Technology-CSIR, Trivandrum, India
- 4Thermo-Catalytic Processes Area (TPA), Material Resource Efficiency Division, Indian Institute of Petroleum-CSIR, Dehradun, India
- 5Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India
- 6Institute of Life Sciences, NALCO Square, Bhubaneswar, Odisha, India
Phragmites is the tallest energy crop found as an invasive species worldwide and considered as waste biomass. The present study evaluated the potential of the aquatic biomass Phragmites karka grown from two different lakes as feedstock for biofuel production. A comparative study of biomass was conducted from Chilika Lake and Loktak Lake, India. The methodology involves dilute acid, sono-assisted alkali pretreatment, and hydrolyzed biomass with commercial cellulase. SEM, XRD, and FTIR analysis were performed for the biomass physicochemical studies and confirmed that alterations occurred in the biomass structure, assisting the hydrolysis process. The enzymatic hydrolysis result showed that the highest of reducing sugar yield of 79% was obtained from biomass loading of 10% and 1% w/v alkali with a sonication frequency of 20 kHz for 25 min. Acid pretreatment released maximal reducing sugar yield of 73% attained from biomass loading of 20% and 0.5% w/v acid. Composition analysis of biomass showed that cellulose content increased from 36% to 46%. Sono-assisted alkali pretreatment solubilized 40% of lignin content compared to untreated biomass. Final ethanol recovery from the biomass is 78% fermentation efficiency from glucose. The data indicate that exploiting tall reed grass as a bioenergy raw material can be a viable approach for sustainable utilization of invasive grass/waste biomass for biorefineries, which helps control invasive weeds and management of waste.
Worldwide energy demand is increasing, and its soaring price leads to looking for an alternative sustainable solution for fossil fuels. Biomass from lignocelluloses is feasible alternatives to fossil fuels for solving energy demands for the future. The lignocelluloses biomass is a potent source of sugars, and availability with energy vs. food competition status made it an inexpensive and readily available raw material for biofuels (Sivagurunathan et al., 2017; Baruah et al., 2018). Lignocellulosic biomass predominantly comprises cellulose, hemicellulose, and lignin joined together in a compact structure. The tight binding of these molecules will give recalcitrance to the biomass, which will affect the enzymatic efficiency during hydrolysis (Sun et al., 2016; Sivagurunathan et al., 2017; Sahoo et al., 2018). The current cost of ethanol from cellulosic biomass is comparatively higher due to the cost of enzyme and low hydrolysis efficiency (Sukumaran et al., 2010; Zhu et al., 2015). Therefore, different pretreatment strategies are employed to reduce biomass recalcitrance by breaking the linkages between cellulose, hemicelluloses, and lignin complex. However, different pretreatment approaches such as physical, chemical (either acids or alkali), and combined physiochemical treatment and biological pretreatments were evaluated to meet the target as mentioned earlier (Ravindran and Jaiswal, 2016; Liu and Bao, 2019; Hassan et al., 2020). In addition to this, structural alterations of lignin and cellulose are required to increase cellulase enzyme accessibility to cellulose for releasing sugars. Pretreatment steps can also aid in degrading/remove lignin and hemicelluloses, which is also a contributing factor for improving enzyme efficiency (Huang et al., 2019). Hence, the pretreatment steps disorganize the cellulosic fiber and cellulose polymers exposed to the surface, which assists efficient enzyme action.
Low-cost pretreatment methods are highly preferred in lignocellulosic biorefineries for better hydrolysis efficiency for biofuel and other platform chemicals. Characteristics of suitable pretreated biomass for enzymatic hydrolysis are the increase of pore size for enzyme infiltration and less lignin with crystallinity, which helps minimize the physical barriers of biomass and avoids association of enzyme with lignin (Kim et al., 2015). Commonly preferred pretreatment process for biomass is alkali, steam explosion, and acids (Soares et al., 2016; Sindhu et al., 2017; Sahoo et al., 2018; Huang et al., 2019). Alkaline pretreatments break the ester linkages between hemicelluloses and lignin, which further helps for the solubilization of lignin and hemicelluloses. This step helps to expose cellulose to the surface (Balat et al., 2008; Bensah et al., 2019). While dilute acid pretreatment disintegrates the hemicelluloses efficiently, this process generates some toxic compounds (hydroxymethyl furfural and furfural) produced due to the degradation of C5 and C6 sugars, which affect the fermentation process (Zhong et al., 2017). Hence, chemical treatment of either acids or alkali is not efficient and time-consuming; therefore, combined physicochemical pretreatment of alkali with ultrasound is preferred due to better efficiency (Zhong et al., 2017; Hassan et al., 2020). The energy from sonication gives unique physicochemical surroundings for processing biomass (Ensminger and Bond, 2011). It could act on the lignin–hemicellulose barriers and break the linkages. The process assists chemicals used for pretreatment to reach the cellulosic fibers and increase the cellulose accessibility by enzymes (Koutsianitis et al., 2015). The sonication process is a potential alternative pretreatment for lignocellulosic biomass for enhancing sugars for biofuels (Bussemaker and Zhang, 2013; Hassan et al., 2020; Ong and Wu, 2020).
Reed grasses are perennial grass, one of the promising energy crops due to its fast growth, widely spread in wetland, and terrestrial habitat. These grasses are resistant to harsh environmental conditions such as prolonged drought, flood, contaminated soil, and degraded lands. Due to its vigorous growth and difficult to control, some parts of the world reed are considered an invasive weed. This grass is recently given more attention as a promising renewable energy source due to its fast growth and biomass production (Cavalaglio et al., 2016; Pandey and Maiti, 2020; Zhao et al., 2020).
The natural freshwater source in Northeastern India is Loktak Lake, and the beauty of the lake is the presence of floating wetlands called phumdis, found only in Manipur. In contrast, Chilika Lake is a brackish water coastal wetland of Odisha State, India. Phumdis of Loktak and Chilika Lake found abundant availability of aquatic invasive tall reed grass (Phragmites karka). Local people removed this biomass due to uncontrollable growth in the lake and dumping on the shore of the lake. This grass invades and outcompetes with indigenous native species of the lake. Hence, overgrowth of these tall reed grasses in these lakes loses its natural and economic values due to disruption in biodiversity and ecology. One possible option for controlling tall reed grasses is the commercial exploitation of these grasses for biofuels and other value-added products, which will help waste management (Zhao et al., 2020). The present study illustrates the comparative evaluation of tall reed grass from Loktak Lake and Chilika Lake as feedstock for bioethanol conversion. Dilute acid and sonication-assisted alkali pretreatments were conducted. The enzymatic hydrolysis was done to assess the digestibility of pretreated biomass. Pretreated biomasses are characterized by crystallinity and structural alteration analysis with the help of SEM, FTIR, and XRD. Fermentation studies for ethanol conversion of hydrolysate obtained from the biomass were performed using wild yeast cells, and sugar consumption and ethanol production were analyzed from the samples collected.
Experimental Materials and Methods
Biomass of tall reed grass was collected from Loktak Lake and was dried in an oven at 50°C for 48 h. The size reduction of biomass was carried out to get a particle size of 1 mm. Chilika Lake biomass is provided by the Institute of Life Sciences, Bhubaneswar, Odisha. Milled biomass was homogenized and stored in the laboratory. The moisture content of powdered biomass was 9.8%.
The pretreatment experiment of biomass was carried out in a 250 ml beaker containing biomass loading of 10% and 1% (w/v) of alkali (NaOH). The reaction mixtures were used for sonication using a sonicator (Sonics Vibra cells, Kolkata) with a prob tip diameter of 1.0 cm and frequency of 20 kHz with an output power of 650 W for 15–40 min. Acid pretreatment of biomass was conducted with 10 and 20% biomass loading with 0.5, 1.0, and 2%w/v of H2SO4. The biomass is washed using tap water until neutral pH. The separated solid fraction was dried and stored for further use.
Biomass Chemical Composition
The chemical composition of tall reed grass of pretreated and untreated samples was performed to know the component such as cellulose, hemicellulose, and lignin as per NREL analytical protocol (Sluiter et al., 2011). Sugars obtained after the hydrolysis process were quantified and analyzed with HPLC having an RI detector (Shimadzu Prominence UFLC) and Rezex ® RPM monosaccharide column (Phenomenex). Deionized water (mobile phase) was used with a flow rate of 0.8 ml/min and 80°C temperature maintained for the oven.
Electron microscopy is conducted to evaluate any changes that occurred with the surface of pretreated biomass. The analysis of the samples was performed with an electron microscope of Zeiss Evo 17 SE, Germany.
FITIR analysis was performed to understand the structural variations in functional groups of pretreated biomass using a Shimadzu IRT racer-100 spectrometer (Japan). Approximately, 2 mg of biomass samples was mixed with 100 mg of KBr. A pellet of 12 mm was prepared by pressing mixture in a standard device using 600 psi. The spectrum obtained for potassium bromides was subtracted from sample spectra. FTIR gives information on the structure and bonding nature of biomass. The spectra were attained from 20 scans of average with a resolution of 0.25 cm−1with in the array of 4000-600 cm−1.
The biomass crystallinity analyses of pretreated and untreated samples were performed with an X-ray diffractogram (PANalytical, the Netherlands). Pretreated and untreated dried samples are powdered and pressed into a tablet. X-ray diffractograms (XRDs) are recorded from 5° to 40° with a difference of 0.03° with the Ka/Cu energy of X-ray (l ¼ 1.54 Ao) produced with a voltage of 40 kV and current of 30 mA. The calculation of crystallinity was performed based on the equation as follows (Segal et al., 1959):
CrI(%) = [I002−IAM/I002]×100,
where IAM represents the peaks for amorphous cellulose and I002 represents the peak of the crystalline part.
The biomass saccharification of pretreated and untreated tall reed samples was conducted in a150 ml flask. The hydrolysis was carried out with 20FPU/g biomass of acid cellulase (Zytex Pvt. Ltd, Mumbai, India). The activity of different enzymes available in this enzyme cocktail was reported previously by Puthiyamadam et al. (2019). Biomass loading of 2% (w/w) along with 200 µL of 10 X antibiotic solution (Himedia), tween 80 as a surfactant (0.1%), and 0.1M citrate buffer was used for the final volume up to 20 ml. The reaction mixture was kept in a water bath (50°C) with 100 rpm for 48 h. Samples are collected for sugar estimation from 0 h to 48 h, centrifuged, and the supernatant was used for evaluation of glucose by DNS assay, according to Miller (1959).The efficiency of enzymatic hydrolysis was calculated by the equation reported by Huang et al. (2019).
Fermentation of Hydrolysate
Fermentation was carried out from the concentrated hydrolysate of sono-alkali pretreated biomass. The experiment was performed in a 20 ml glass vial with 10 ml hydrolysate as a working volume. The vial was inoculated with a 4% (wet v/v) 24 h old culture of wild yeast SFAY2 and incubated under static conditions at 30°C ± 2 for 48 h. Samples were collected and analyzed for sugar depletion and ethanol concentration.
Result and Discussion
Biomass Composition of Tall Reed Grass
Biomass component analysis showed that Loktak Lake tall reed grass contains cellulose content 37%, hemicellulose 20%, and lignin 24.6%. While in the case of Chilika Lake, biomass consists of cellulosic material 34.7, hemicelluloses 19.7%, and lignin 27.9%, respectively. Variation in the composition could be due to the biomasses are from different lakes and climatic conditions, which could affect the biomass composition. Composition analyses of native Phragmites karka collected from two different lakes are shown in Table 1. Composition analysis supports an earlier report on Phragmites (Cavalaglio et al., 2016). Sonication-assisted alkali-pretreated biomass from Loktak Lake showed that cellulosic content increased by 45%. The hemicelluloses content reduced to 13.8% and lignin solubilization to 40%. While sonication-assisted alkali pretreatment of Chilika Lake biomass showed, cellulosic content increased from 34.7% to 40%, hemicellulose reduction to 10.8%, and lignin reduced to 30%. The biomass pretreated with dilute acids showed cellulose content 41%, hemicellulose 6.3%, and lignin content 24% obtained from Loktak biomass. Meanwhile, Chilika Lake acid-treated biomass showed a cellulose content of 39%, hemicellulose 4%, and 28% lignin. The compositional changes of Phragmites karka samples after different pretreatment processes are represented in Table 2. Usually, acid pretreatment of biomass solubilizes the hemicellulose, improving enzymatic digestibility (dos Santos et al., 2018). Sono-alkali pretreatment is an attractive process that selectively acts on the lignin and hemicellulosic portion of biomass and helps for an increase of cellulosic content. Hence, it decreases the lignin and hemicellulose of biomass (Zhu et al., 2015; Hassan et al., 2020). Hemicellulose and lignin contents of biomass inhibit cellulase enzyme action on cellulose. This disintegration can enhance accessible surface and increase pore size for cellulose accessibility and aids in improved efficiency of hydrolysis (Sindhu et al., 2017; Wu et al., 2017). Sonication-assisted alkali treatment is recently reported in the case of feedstocks from brewers spent grain, wheat straw, sugarcane bagasse, grass clipping, and chili postharvest residues (Sindhu et al., 2017; Wang et al., 2017; Zhong et al., 2017; Hassan et al., 2020). Reports are limited in the literature for the exploitation of Phragmites as feedstock for biofuel.
Scanning Electron Microscopy Analysis
Pretreatment can affect the structure of lignocellulose. SEM analyzes the alterations in the morphological structure of untreated and pretreated biomass. Untreated biomass of tall reed grass has an intact, organized, and smooth surface without any erosion (Figure 1A). Biomass pretreated with dilute acids showed that fibers are ruptured and detached, which further aid in the exposure of active site in the biomass for hydrolyzing enzymes (cellulase) (Figures 1D,E). In the case of ultrasound-assisted alkali-pretreated biomass is rougher and fibers are entirely disorganized, cellulosic fiber bundles are exposed to the surface, and swelling nature is observed (Figures 1B,C). This further helps expose more cellulose fiber that assists in an increase in cellulase accessibility for the release of more fermentable sugars. Identical observations are also reported by Wang et al. (2017) for grass clipping, brewers spent grains by Hassan et al. (2020) and Ramadoss and Muthukumar (2016) for sugar cane bagasse.
FIGURE 1. Scanning electron microscopic images of pretreated biomass. Legends: (A) native, (B) sono-alkali Chilika Lake, (C) sono-alkali Loktak Lake, (D) acid Chilika Lake, and (E) acid Loktak Lake.
Enzymatic digestibility of pretreated biomass depends on the alterations of chemical bonds, structure, and quality of the pretreatment process. The biomass functional groups and structural alteration of pretreated biomass were analyzed by FTIR (Figures 2A,B). The absorption peak positions were assigned according to the literature on biomass chemical composition (Chung et al., 2004; Raiskila et al., 2007; Wang et al., 2011; Pandiyan et al., 2014). Lignin-related information was obtained from the FTIR band spectra around 1850–600 cm−1. Higher lignin reduction was observed in sonication-assisted alkali-pretreated Phragmites samples than in acid pretreatment, further supporting changes in the surface morphology of biomass obtained from SEM data. The intensity band reduction at 897 cm−1provides cellulose details, especially β–D linkages of glycosidic bonds and the absorption spectra from 1,635 to 1,640 cm−1, representing the vibrations of water bending (Borirlau et al., 2010). The information regarding stretching C-O bonds of lignin guaiacyl group, hemicellulose, or cellulose was obtained from peaks at 1,037 cm−1(Chung et al., 2004). The C=O of ketone groups showed a sharp intensive peak around 2,350 cm−1(Pandiyan et al., 2014). CH stretching-related information was obtained at 2,800–3,000 cm−1, and OH stretching of cellulose represented spectra around 3,550–3,100 cm−1 (Chung et al., 2004). The alterations of absorption peaks of pretreated biomass could affect the crystallinity, which is confirmed by XRD.
FIGURE 2. FTIR spectra of native and pretreated samples of tall reed grass: (A) Chilika Lake and (B) Loktak Lake.
X-Ray Diffraction Analysis
Crystallinity index analysis is an important factor used to assess the hydrolytic process of pretreated biomass. The crystallinity of biomass is calculated according to the formula provided by Segal et al. (1959). It found that the crystallinity index of native biomass was 33.5–34.8%. It further increased to 62% in sono-assisted alkali-treated biomass of Chilika Lake and Loktak Lake (60%). For the acid-treated biomass, the crystallinity index increased from 54 to 56% from Chilika Lake and Loktak Lake biomass. CrI signifies the comparative numbers of crystalline cellulose availability in total solids of biomass. The crystallinity index of pretreated biomass of tall reed grass is represented in Figure 3. The removal of amorphous lignin is reflected in enhancing crystallinity in pretreated biomass. Higher crystallinity index in sono-assisted alkali pretreatment could be explained by removing lignin and exposing the biomass cellulosic content compared with acid-pretreated and untreated biomass. Hence, the maximum value in sono-alkali pretreatment confirms that sono-assisted pretreatment is better for biomass processing for ethanol. This observation agreed with an earlier report of an increase in CrI values during sono-assisted pretreatment of rice straw (Wu et al., 2017), grass clipping (Zhong et al., 2017), and cotton stalk (Wang et al., 2016).
The saccharification process of pretreated samples is an essential step in the lignocellulosic biorefinery. The yield of sugars from pretreated feedstock mainly depends on enzymes used for hydrolysis, active site presence, and the biomass porosity. These characters further depend on the loading of the enzyme and hydrolysis duration, which will affect the bioprocess (Sukumaran et al., 2010). The efficiency of the pretreatment process is further evaluated by monitoring the susceptibility to enzymatic hydrolysis. The pretreated biomass was subjected to hydrolysis by using 20FPU/g of cellulose. Sugar yields were analyzed by the DNS method. Hydrolysis of biomass pretreated with different alkali concentrations ranges from 0.5, 1, and 1.5% (w/v) and produces reducing sugar yield from 12, 20, and 15%, respectively. The highest yield of sugar was obtained from biomass pretreated with 1% (w/v) of alkali. This concentration was chosen further for the sonication-assisted alkali pretreatment. Hydrolysis of sono-alkali treated biomass with different time intervals showed that reducing sugar yield increased with increasing time. The highest enzymatic digestibility of sono-alkali pretreated biomass with 48–79% reducing sugar yields was obtained from biomass loading of 10 and 1% w/v alkali with a sonication frequency of 20 kHz for 25 min from Loktak Lake biomass. For the enzymatic digestibility of Chilika Lake biomass, maximum reducing sugar yield obtained is 75% from alkali-treated biomass with a sonication frequency of 20 kHz for 25 min (Figures 4A,B). Results agreed with the earlier report on ultrasound-assisted alkali pretreatment of grass clipping (Wang et al., 2017). Acid-pretreated biomass showed that maximal reducing sugar yield of 72–73% was attained from 10% to 20% biomass pretreated with 0.5–1% (w/v) dilute acid from Loktak Lake and Chilika Lake biomass, respectively (Figures 5A,B). The differences in the reducing sugar yield from different pretreatment process are due to the ratio of delignification and solubilization of hemicelluloses. An earlier report showed that reduction in enzymatic hydrolysis yield is due to physical barriers of lignin and nonproductive adsorption of enzymes (Huang et al., 2019). Huang et al. also reported that the accessibility of pretreated biomass was enhanced and further improved the digestibility of biomass with enzymes, supporting with the present study. Higher reducing sugar yield from pretreated biomass might be due to more pore size, the surface area of cellulosic content, decreased lignin, and hemicellulosic content of biomass (Baruah et al., 2018; Lin et al., 2020). It has been reported that the application of ultrasound during the pretreatment process acts as an auxiliary source of energy for solid–liquid separation of bioprocess technology, mass transfer, and diffusion. This is mainly due to microbubble formation during sonication-assisted pretreatment (Gracia et al., 2011). Velmurugan and Muthukumar (2012) showed that higher reducing sugars from ultrasound-assisted pretreated biomass are due to cavitation formation in aqueous media, which creates high shear forces, pressure, and temperature. Cavitation facilitates the formation of free radicals from water molecules breakdown, which help to break the network of hemicellulose and lignin (Iskalieya et al., 2012).
FIGURE 4. The enzymatic hydrolysis efficiency of sono-assisted alkali-pretreated tall reed biomass: (A) Chilika Lake and (B) Loktak Lake.
FIGURE 5. The enzymatic hydrolysis efficiency of acid-pretreated tall reed biomass: (A) Loktak Lake and (B) Chilika Lake.
Fermentation of hydrolysate from Phragmites biomass is done without further detoxification process with wild yeast. After fermentation, it is shown that ethanol concentration was 3.4% with a fermentation efficiency of 78.6%, which is calculated based on the theoretical yield of ethanol after 48 h. The less efficiency might be due to inhibitory compounds produced due to the degradation of sugar molecules during the pretreatment process and hydrolysis, affecting microorganism performance during the fermentation process. The results supported with the previous reports from Phragmites australis (Gao et al., 2014). Puthiyamadam et al. (2019) reported that hydrolysate obtained from wet biomass processing of Loktak mixed biomass produced a fermentation efficiency of 95% with wild yeast. The efficiency of fermentation may be improved with the optimization of different process parameters. The present study indicates that sonication-assisted alkali pretreatment is a better approach for obtaining sugars for biofuel (bioethanol) conversion. Furthermore, the exploitation of P.karka for bioenergy application is a feasible alternative solution for controlling invasive grass and the management of waste biomass.
A sustainable analysis of Phragmites karka biomass as raw material for biofuels and biorefinery products needs to be evaluated in future studies.
The invasive aquatic tall reed grass found in Loktak Lake and Chilika Lake is a promising feedstock for biofuel production without compromising food crops. Sonication-assisted alkali pretreatment is useful to improve the saccharification process of Phragmites karka. Results showed that ultrasound alkaline pretreatment significantly enhanced the solubilization of lignin and hemicellulose, reflected in cellulose increased crystallinity. Hence, sono-alkaline pretreatment is superior to the acid pretreatment process, and the efficiency of ethanol produced is 78.6% with the theoretical maximum. However, utilization of tall reed grass as a biorefinery energy crop from these regions can be a solution for addressing control, exploiting tall reed grass for people’s socioeconomic benefit and managing waste biomass for industrially important products.
Data Availability Statement
The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.
SU contributed to experiment, data collection, visualization, and writing original manuscript—review and editing. DS was responsible for conceptualization and formal analysis. AP, VA, and RS performed compositional analysis and characterization of biomass. TB carried out physicochemical analysis of the structure of biomass. AP was involved in providing data about Phargmites from Chilika Lake.
This study was supported by the Institute of Bioresources and Sustainable Development Core Fund.
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
SU is thankful to the Director of IBSD for his motivation and support for this work. The authors are grateful to Microbial Processes Technology for their extensive help in FTIR analysis, the Electron Microscopy Section for SEM, and the Chemical Sciences Division of NIIST-CSIR, Trivandrum (MS no. IBSD/2020/01/032), for XRD analysis.
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Keywords: Phragmites, sonication, pretreatment, Chilika Lake, Loktak lake
Citation: Ummalyma SB, Sahoo D, Pudiyamadam A, Adarsh VP, Sukumaran RK, Bhaskar T and Parida A (2021) Sono-Assisted Alkali and Dilute Acid Pretreatment of Phragmites karka (Tall Reed Grass) to Enhance Enzymatic Digestibility for Bioethanol Conversion. Front. Energy Res. 8:594452. doi: 10.3389/fenrg.2020.594452
Received: 13 August 2020; Accepted: 02 November 2020;
Published: 08 April 2021.
Edited by:Dinh Duc Nguyen, Kyonggi University, South Korea
Reviewed by:Baskar Gurunathan, St. Joseph’s College of Engineering, India
Caoxing Huang, Nanjing Forestry University, China
Copyright © 2021 Ummalyma, Sahoo, Pudiyamadam, Adarsh, Sukumaran, Bhaskar and Parida. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Sabeela Beevi Ummalyma, email@example.com