ORIGINAL RESEARCH article
An experimental test of the role of structural blue and melanin-based chestnut coloration in aggressive contests in male eastern bluebirds
- Department of Biological Sciences, Auburn University, Auburn, AL, USA
Male eastern bluebirds (Sialia sialis) have feathers with either structurally based blue coloration or melanin-based chestnut coloration, and they hold territories during the breeding season that they defend vigorously. We tested whether the melanin pigmentation or structural coloration of feathers serve as signals during intrasexual aggressive encounters by placing color-modified stuffed bluebirds in male territories. We recorded the time to attack and the intensity of attacks on each model, and we then compared the response to color-enhanced vs. color-reduced models. Male bluebirds attacked models with brighter and more chromatic blue coloration significantly more often than they attacked models with darker and less chromatic blue coloration. In contrast, the darkness of the chestnut breast coloration did not have a significant effect on the rate at which models were attacked. We conclude that territorial male bluebirds perceive intruding males with brighter blue coloration as a greater threat than males with drabber blue coloration, presumably because blue coloration is a signal of fighting ability. In contrast, the chestnut coloration of breast feathers appears to be a signal of gender and sexual maturity and not a graded signal of social status.
Sexual selection on a trait can arise either through female mate choice for the trait or through advantages that result from the trait in male-male interactions (Darwin, 1871; Andersson, 1994; Hill, 2014). The means by which armaments like horns, spurs, or large body size can facilitate competition for mates is straightforward and has never been disputed (Cronin, 1991). Less intuitive is the mechanism by which visual displays can arise through male-male competition. There is now an abundance of evidence from studies of diverse taxa that visual displays can serve as signals of male fighting ability and that these signals can mediate contests between males (Andersson, 1994; Berglund et al., 1996; Tibbetts, in press).
Within the context of competition among all males in a population, both dominant and subordinate males benefit from honest signaling of status (Rohwer, 1982; Senar, 2006). Through status signaling, dominant males avoid wasting time and energy engaging in contests with lower-status males, and subordinate males benefit by avoiding dangerous interactions with dominant individuals (Rohwer, 1975; Lyon and Montgomerie, 1986; Hawkins et al., 2012). In a status-signaling system, cheating is prevented either by the high cost of constructing or maintaining an ornament (Zahavi, 1975; Owens and Hartley, 1991) or by the high social costs of dishonestly signaling status (Rohwer, 1977; Ketterson, 1979; Moller, 1987; Tibbetts, in press). Therefore, status signals can be used by birds as honest indicators of a rival's age, rank, or fighting ability (Fugle et al., 1984; Jarvi and Bakken, 1984; Senar, 2006).
In species that defend exclusive territories containing critical resources, all territory-holding individuals are winners with good fighting ability (Lack, 1968; Ligon, 1999; Hill, 2010). Among such males, the benefits of retaining a territory are very high as are the costs of losing a territory. The entire lifetime reproductive success of a territorial male bird often hinges on its ability to defend and retain its territory (Lack, 1968; Ligon, 1999). Assessments of rivals made by territorial males in such circumstances do not concern whether or not to fight; they are about how aggressively they fight. Males signaling high fighting ability pose the greatest threat to a territorial male and are predicted to elicit the strongest response. Males signaling reduced fighting ability pose less of a threat and are predicted to be met with a less intense response (Ligon, 1999; Hill, 2010).
We studied the role of plumage coloration in aggressive interactions in the eastern bluebird (Sialia sialis). Male eastern bluebirds have bright blue coloration on their heads, backs, tails, and rumps and bold chestnut coloration across their breasts (Figure 1). The structural blue coloration results from coherent scattering of light from the spongy layer of feather barbs (Shawkey et al., 2003; Prum, 2006), while chestnut breast coloration results from melanin pigmentation (McGraw et al., 2004). Most structural plumage coloration reflects light in the ultraviolet (UV) region of the spectrum as well as the visible spectrum (Prum, 2006). Female songbirds can perceive both the human-visible and UV light reflected from feathers (Hart et al., 2000; Hunt and Peichl, 2014). Previous research has shown that, within populations, males show substantial variation in the brightness and chroma of blue coloration as well as in the brightness of chestnut breast coloration (Siefferman and Hill, 2003).
Figure 1. A male eastern bluebird in Auburn, Alabama. The two prominent ornaments of eastern bluebirds are the bright blue dorsal plumage and bold chestnut breast plumage. Photograph by Geoffrey E. Hill.
Male eastern bluebirds are highly territorial during the breeding season (Gowaty and Plissner, 1998). Successful males defend territories that contain a nesting cavity, without which they cannot breed, as well as space for exclusive foraging (Gowaty and Plissner, 1998; Siefferman and Hill, 2005b). Male bluebirds are highly aggressive in defense of territories. They permit no other males in their defended space (Gowaty and Plissner, 1998). Removal experiments indicate that male bluebirds that hold territories are a subgroup of all males in the local population. Vacated territories are quickly filled by males who previously held no territory (Siefferman and Hill, 2005b). In an experiment in which a first set of boxes were placed in fields a month before a second set of boxes was added, males that won occupancy of the first boxes had significantly brighter blue coloration than males that occupied boxes placed later (Siefferman and Hill, 2005b). This experiment suggests that blue color mediated dominance interactions among eastern bluebirds, but the role of individual variation in blue and chestnut color in aggressive interactions has not been tested directly in bluebirds.
To test the role of blue and chestnut plumage coloration in assessments of fighting ability, we manipulated coloration on taxidermic models of eastern bluebird males and then presented the models to resident bluebirds during the breeding season. We first manipulated blue coloration while holding chestnut color constant and then manipulated chestnut coloration, while holding blue coloration constant. In our manipulations of blue coloration we matched natural light patterns of light absorption and reflection in both the UV and human visible portions of the spectrum. Bluebirds display threatening postures including bill wiping, wing flapping, and singing toward taxidermic mounts in their territory and will violently attack a model bluebird that remains in the territory (Hill, 2010). We predicted that if individual variation in plumage coloration functions as a signal of fighting ability, then territorial male bluebirds would show a heightened aggression toward more ornamented intruders (brighter or more chromatic blue coloration and darker chestnut coloration). By using models, we reduced the problems of interactive behavior between live models and resident pairs that can override any potential signal function of coloration (Senar, 2006). Furthermore, by artificially manipulating the blue and chestnut coloration of eastern bluebirds, we tested for the importance of structural and melanin feather coloration independent of each other and independent of the age or body condition.
We studied eastern bluebirds on agricultural fields and pastures adjacent in Lee County, Alabama (32° 35'N, 82° 28'W; see Siefferman and Hill (2003) for details of study area). We conducted behavioral experiments during egg laying and chick rearing of first broods when male aggression is high (Gowaty and Wagner, 1988). Males are territorial and will attack conspecifics that encroach upon their territory. To test whether the brightness of male bluebird coloration affects aggressive behaviors in territorial males, we used taxidermic mounts constructed from salvaged carcasses of eastern bluebirds as models to which we artificially manipulated the brightness of the plumage.
In 2009, we increased the brightness and chroma of the structurally based blue coloration on the wings, backs, and tails of six models with a violet marker (Prismacolor® PM-60: violet mist) and decreased the coloration with a black permanent marker (Sharpie® permanent marker: black) following the procedures in (Liu et al., 2007) on six models. To a human observer, there is little variation in coloration among male bluebirds and our treatments appeared to have subtle effects on the appearance of the models. This poor perception of structural coloration is likely a consequence of most of the variable wavelengths falling outside or near the periphery of human color perception. Spectrometric analysis showed that our manipulations pushed males toward the extremes of bright and drab coloration observed within populations (Figure 2). The degree of color change caused by our marker treatment would have been readily visible to an eastern blue bluebird (Liu et al., 2007).
Figure 2. Mean spectral reflectance of blue rump feathers of eastern bluebirds, including unmanipulated males (gray line), artificially brightened models constructed from male carcasses (blue line), and artificially darkened models constructed from male carcasses (black line). Error bars show 95% confidence intervals.
In 2010, we increased the chroma of the melanin-based chestnut coloration on a male bluebird's breast using a brown marker (Prismacolor® PM-90: Walnut) and decreased the coloration using hydrogen peroxide (White et al., 1980) to bleach the feathers. The effects of our color manipulation on breast coloration was more obvious to a human observer than were the effects of blue manipulation, likely because all of the color variation occurred within wavelengths that are readily perceived by humans. The effect of the breast color manipulation as measured by a spectrophotometer, however, was smaller (Figure 3). In both cases, we pushed coloration toward the extremes of dark and light plumage seen in our study population (Figures 2, 3), so our tests of the responses to the models should be relevant to how wild male eastern bluebirds respond to brightly or drably colored intruders. As with the tests of structural coloration, we used six different pairs of models to minimize the influence of the idiosyncrasies of any single model.
Figure 3. Mean spectral reflectance of chestnut breast feathers of eastern bluebirds, including unmanipulated males (gray line), artificially brightened models constructed from male carcasses (tan line), and artificially darkened models constructed from male carcasses (brown line). Error bars show 95% confidence intervals.
To measure the effect that our manipulation had on feather coloration, we quantified the coloration of feathers using an Ocean Optics S2000 spectrometer (Dunedin, Florida). We took measurements at 90° incident and reflective angles. For both blue and chestnut coloration, we calculated brightness as the mean summed reflectance (R300−700 nm). For blue coloration, we calculated UV chroma as the proportion of the total reflectance (R300−700 nm) in the UV part of the spectrum (R300−400 nm). Color parameters are correlated in eastern bluebirds such that the most-ornamented UV-blue males display brighter coloration, greater UV chroma, and hues with wavelengths shifted toward the shorter wavelengths (Siefferman et al., 2005).
During both years we followed the same protocol: we drove two, 1.2 m metal poles into the ground 5 m from a bluebird nest box and 5 m apart. Atop each pole we placed a taxidermic bluebird model attached to a 1.2 m wooden dowel (diameter 1 cm) placed perpendicular to the pole. The dowel gave responding bluebirds a place to perch near the model and display before attacking. One darkened model and one brightened model were simultaneously displayed to a breeding pair while a speaker repeatedly played a 30 s clip of bluebird songs between them. We randomly chose one model from the bright category and one model from the drab category for each trial. Thus, there were 36 potential model combinations.
Models were covered with a paper bag and then simultaneously exposed by pulling the bags off the models using thin fishing line (following procedures in Ligon and Hill, 2009). We played bluebird songs for 5 min before exposing the models and throughout the trial to ensure that the resident bluebirds were attentive to the models and searching for an intruding adult male before the models were presented. Observations were made from a concealed location >30 m from the nest box.
The trial began and we started recording bluebird behaviors when the models were exposed. We stopped trials after 10 min or when a model was physically attacked to preserve model condition for later trials, and models remained undamaged through the experiment. We quantified which model received the first signs of aggression and which model received the most aggressive attacks. We also recorded the amount of time until the models were attacked. We did not calculate the repeatability of male behaviors by exposing individual males more than once. Exposing males to the models multiple times would have induced variable levels of habituation that would have made it hard to estimate heritability and simulating multiple intrusion would have unduly harassed individual males.
We used a Chi-squared test to compare the observed number of times the brighter model was attacked first relative to our expected, or null hypothesis, that color did not impact which model was attacked first. In trials where the model receiving the most aggression was dissimilar to the model that was first attacked, we used a Fisher's exact test to determine if the change was random of the switch more likely to be from the drab model to the bright model than vise versa. A Chi-squared test was also used to determine ifdarker breast coloration differed from parity.
We presented models with manipulated blue coloration to 66 different territorial males. Seven of these the 66 males did not approach either of the models and we not observed during the trial period, and we excluded those seven males from our analyses. Of the remaining 59 trials, resident males first attacked the blue brightened model in 39 trials and the drabber blue model in 20 trials (X2 = 4.90, N = 59, P = 0.03, Figure 4). The blue brightened model was attacked most aggressively in 48 of the 59 trials (81%). Among the 11 trials in which the model receiving the most aggression differed from the model receiving the first aggression, the responding male switched the focus of attack from the drab to the bright model in six trials (55%) and from the bright to the drab model in five trials (45%), indicating that the attacks of these males were not focused on a particular model (Fisher's exact test, P = 0.99).
Figure 4. Number of trials in which a male bluebird model with either brightened or darkened blue feathers was attacked first. (N = 59).
We presented models with breast color manipulated to 36 different territorial males. The marker treatments on breast plumage had no significant effect on the responses of territorial males. Resident males first approached the model with darker chest coloration in 16 trials, and they first approached the model with lighter chest coloration in 20 trials (X2 = 1.78 N = 36, P = 0.18; Figure 5).
Figure 5. Number of trials in which a male bluebird model with either lightened or darkened chestnut breast feathers was attacked first. (N = 36).
Previous studies showed that blue coloration but not chestnut coloration is associated with individual quality in eastern bluebirds. Brightness and chroma of structural blue coloration of males was correlated with earlier breeding and greater number of offspring fledged (Siefferman and Hill, 2003, 2005a,b); juveniles in less crowded nests grew brighter feathers than juveniles in more crowded nests (Siefferman and Hill, 2007); and, females subjected to food stress grew less colorful feathers than females that were not food stressed (Siefferman and Hill, 2005a). Studies of other species of passerine birds have also found positive relationships between individual condition and the brightness or chroma of structural coloration but rarely between condition and expression of chestnut phaeomelanin coloration (reviewed in Hill, 2006).
Previous tests of the function of blue coloration in birds have generated ambiguous results. Experiments with blue tits (Cyanistes caeruleus) and European starlings (Sturnus vulgaris) revealed female mate choice for bright and chromatic structural coloration (Bennett et al., 1997; Andersson et al., 1998), but a subsequent study with blue tits found no choice for UV crown coloration (Kurvers et al., 2010). Controlled mate choice experiments with blue grosbeaks (Passerina caerulea) and eastern bluebirds demonstrated that females of those species do no use male structural coloration as an important criterion in mate choice (Ballentine and Hill, 2003; Liu et al., 2007, 2009). Studies of dominance in relation to structural plumage coloration have been conducted on blue tits, and various studies both supported and refuted the hypothesis that brighter blue/UV coloration is a signal of fighting ability (Alonso-Alvarez et al., 2004; Korsten et al., 2007; Vedder et al., 2010).
Given that blue coloration of eastern bluebirds is known to relate to individual condition and that female bluebirds do not appear to use male coloration as a criterion in mate choice, we hypothesized that blue coloration may play a role in signaling resource holding potential. In support of this hypothesis, we found that male eastern bluebirds altered their aggressive behavior toward models in response to experimental modifications in structural-based blue feather coloration. Males attacked conspecific models that were artificially brightened significantly more often than they attacked artificially dulled models.
The observation that male fighting ability is signaled by the brightness and chroma of blue structural coloration is consistent with observations from previous studies of blueness and territory acquisition by male eastern bluebirds on the same study area. In an experiment in which nest boxes were placed in pastures that represented excellent bluebird breeding habitat, but that lacked any nest sites, males that gained ownership of the first set of boxes were significantly brighter and more chromatic than males that won boxes erected later (Siefferman and Hill, 2005a,b). Presumably, brighter males acquired the first boxes because they held higher social status. The current study provides experimental evidence that blue coloration alters the aggressive behavior of territory males in a pattern consistent with the hypothesis that bluer males have higher resource holding potential.
In contrast to the significant effect of structural coloration of models on the rates at which they were attacked, differences in chestnut breast coloration among models did not have a significant affect how territorial males responded to them. Variation in the boldness of the chestnut breast display of eastern bluebirds does not appear to affect how the signal is perceived by conspecifics. These observations may seem at odds with the extensive literature on melanin coloration and social status in which melanin ornaments are consistently positively related to resource holding potential (Senar, 2006). However, the great majority of studies of melanin coloration and dominance have focused on badge size and not coloration per se and most studies focused on black eumelanin coloration rather than brown phaeomelanin coloration (Senar, 2006). Territorial male eastern bluebirds have been shown to respond strongly to models with orange breasts compared to models with plain white or white spotted breasts (Ligon and Hill, 2009). Here we show that, within the range of variation shown by adult males in a population, the chestnut breast coloration of male bluebirds appears not to serve as a graded signal of social status in eastern bluebirds. Taken together, these observations support the idea that chestnut breast coloration of bluebirds serves as a signal of gender and sexual maturity (Ligon and Hill, 2009; Hawkins et al., 2012) and not fighting ability per se.
The implications of our studies of the responses of males to chestnut and blue coloration are that these two color patches serve different but equally important functions. A male bluebird faced with an intruder first assesses breast coloration to gauge whether the intruder is a threat worthy of time and effort by observing whether or not it has an orange breast. If the intruder is a rival males as signaled by its orange breast then the territorial male assesses blueness to gain information about likely fighting ability. Males with brighter blue plumage are signaling higher status and are more of a threat around nests than males with drabber blue plumage. By having no orange feathers, juvenile bluebirds avoid aggression from adult males (Ligon and Hill, 2009). By having breast coloration so drab that it is below the range of variation among males (and below the level of manipulation used in this study), female bluebirds also avoid most male aggression. Only orange-breasted adult males invoke a response from males and that response is adjusted to the fighting ability as signaled by blue plumage.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
This work was supported by grants (IBN 0235778 and DEB 0077804) to Geoffrey E. Hill from the National Science Foundation.
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Keywords: plumage color, structural coloration, melanin coloration, aggression, territoriality
Citation: Mercadante A and Hill GE (2014) An experimental test of the role of structural blue and melanin-based chestnut coloration in aggressive contests in male eastern bluebirds. Front. Ecol. Evol. 2:24. doi: 10.3389/fevo.2014.00024
Received: 09 February 2014; Accepted: 27 May 2014;
Published online: 11 June 2014.
Edited by:Wayne Iwan Lee Davies, University of Western Australia, Australia
Reviewed by:Dustin R. Rubenstein, Columbia University, USA
David M. Hunt, University of Western Australia, Australia
Copyright © 2014 Mercadante and Hill. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Geoffrey E. Hill, Department of Biological Sciences, Auburn University, 331 Funchess Hall, Auburn, AL 36849-5414, USA e-mail: firstname.lastname@example.org