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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Ecol. Evol. | doi: 10.3389/fevo.2019.00437

CRABS CLAW and SUPERMAN coordinate hormone-, stress- and metabolic-related gene expression during Arabidopsis stamen development

 Ze Hong Lee1, Yoshitaka Tatsumi1,  Yasunori Ichihashi2,  Takamasa Suzuki3, Arisa Shibata4,  Ken Shirasu4, Nobutoshi Yamaguchi1 and  Toshiro Ito1*
  • 1Nara Institute of Science and Technology (NAIST), Japan
  • 2RIKEN BioResource Research Center (BRC), Japan
  • 3Chubu University, Japan
  • 4RIKEN Yokohama, Japan

The appropriate timing of the termination of floral meristem activity (FM determinacy) determines the number of floral organs. In Arabidopsis, two transcription factors, CRABS CRAW (CRC) and SUPERMAN (SUP), play key roles in FM determinacy. CRC belongs to the YABBY transcription factor family, whose members contain a zinc finger and a helix-loop-helix domain. The crc mutation causes the formation of unfused carpels and leads to an increase in carpel number in sensitized backgrounds. The SUP gene encodes a C2H2-type zinc-finger protein, and sup mutants produce extra carpels and stamens. However, the genetic interaction between CRC and SUP is not fully understood. Here, we show that these two transcription factors regulate multiple common downstream genes during stamen development. The crc sup double mutant had significantly more stamens and carpels than the parental lines and an enlarged floral meristem. Transcriptome data have implicated several cytokinin- and auxin-related genes as well as stress- and metabolic-related genes to function downstream of CRC and SUP during stamen development. The regulation of common downstream genes of CRC and SUP might contribute to the initiation of an appropriate number of stamens and to subsequent growth and development.

Keywords: Arabidopsis, ARABIDOPISIS HISTIDINE PHOSPHOTRANSFER PROTEIN6, CRABS CLAW, Cytokinin, floral meristem, flower, Superman

Received: 28 Aug 2019; Accepted: 25 Oct 2019.

Copyright: © 2019 Lee, Tatsumi, Ichihashi, Suzuki, Shibata, Shirasu, Yamaguchi and Ito. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Toshiro Ito, Nara Institute of Science and Technology (NAIST), Ikoma, 630-0192, Nara, Japan, itot@bs.naist.jp