In the original article, there were mistakes in Table 2, Table 3, Table 4, Figure 1, Figure 2, Figure 3 and Figure 4.
In all mentioned tables and figures, quantity of amino acids was described in mM instead of μM. The corrected tables and figures appear below. The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way.
The original article has been updated.
Table 2
| Amino acid | Control group | Study group, day 0-3 post-SAH | Control group vs. Study group, day 0-3 post-SAH. P-value | Study group, day 5 post-SAH | Control group vs. Study group, day 5 post-SAH. P-value | Study group, day 10 post-SAH | Control group vs. Study group, day 10 post-SAH. P-value |
|---|---|---|---|---|---|---|---|
| O-phosphoethanolamine (μM) | 3.00 | 5.95 | <0.01 | 5.15 | <0.001 | 6.60 | <0.001 |
| Ethylamine (μM) | 9.80 | 9.40 | 0.798 | 9.65 | <0.001 | 11.10 | 0.292 |
| Taurine (μM) | 7.00 | 16.00 | <0.001 | 14.30 | <0.001 | 10.75 | <0.01 |
| Asparagine (μM) | 5.70 | 10.35 | 0.01 | 19.50 | <0.001 | 21.75 | <0.001 |
| Serine (μM) | 24.20 | 55.70 | <0.001 | 75.40 | <0.001 | 83.75 | <0.001 |
| Glycine (μM) | 8.90 | 38.75 | <0.001 | 34.85 | <0.001 | 35.20 | <0.001 |
| Hydroxyproline (μM) | 0.60 | 1.00 | 0.164 | 1.35 | 0.658 | 1.35 | 0.289 |
| Glutamine (μM) | 407.60 | 520.65 | <0.01 | 791.10 | <0.001 | 796.30 | <0.001 |
| Aspartic acid (μM) | 0.50 | 1.55 | <0.001 | 0.80 | 0.175 | 0.95 | 0.054 |
| Citruline (μM) | 1.60 | 2.75 | 0.011 | 2.55 | <0.01 | 3.30 | <0.01 |
| Threonine (μM) | 24.80 | 29.05 | 0.079 | 56.10 | <0.001 | 63.40 | <0.001 |
| Beta-Alanine (μM) | 20.30 | 22.00 | 0.040 | 29.00 | <0.01 | 27.30 | <0.01 |
| Alanine (μM) | 35.00 | 88.05 | <0.001 | 114.55 | <0.001 | 122.55 | <0.001 |
| Glutamic acid (μM) | 1.20 | 5.40 | <0.001 | 3.00 | <0.001 | 2.35 | <0.01 |
| Histidine (μM) | 11.40 | 28.20 | <0.001 | 43.35 | <0.001 | 40.20 | <0.001 |
| 3-Methylhistidine (μM) | 0.50 | 1.00 | <0.01 | 1.40 | <0.001 | 1.05 | <0.01 |
| 2-Aminoadipic acid (μM) | 0.00 | 1.65 | <0.001 | 2.10 | <0.001 | 1.45 | <0.001 |
| Gamma-aminobutyric acid (μM) | 0.40 | 0.50 | 0.699 | 0.30 | 0.012 | 0.20 | 0.051 |
| 3-Aminoisobutyric acid (μM) | 0.50 | 0.30 | 0.668 | 0.30 | 0.563 | 0.35 | 0.806 |
| 2-Aminobutyric acid (μM) | 3.20 | 6.20 | <0.01 | 6.60 | <0.001 | 7.30 | <0.001 |
| Arginine (μM) | 15.70 | 19.25 | 0.062 | 26.60 | <0.001 | 24.35 | <0.01 |
| Proline (μM) | 0.80 | 11.80 | <0.001 | 13.95 | <0.001 | 16.15 | <0.001 |
| Ornithine (μM) | 4.30 | 19.80 | <0.001 | 17.95 | <0.001 | 17.75 | <0.001 |
| Cystathionine (μM) | 0.30 | 2.05 | <0.001 | 1.05 | <0.001 | 1.40 | <0.001 |
| Cysteine (μM) | 0.40 | 2.05 | <0.001 | 1.75 | <0.001 | 1.65 | <0.001 |
| Lysine (μM) | 22.50 | 53.80 | <0.001 | 73.05 | <0.001 | 72.40 | <0.001 |
| Methionine (μM) | 3.10 | 6.20 | <0.01 | 11.70 | <0.001 | 13.65 | <0.001 |
| Valine (μM) | 16.20 | 38.65 | <0.01 | 66.20 | <0.001 | 76.30 | <0.001 |
| Tyrosine (μM) | 7.80 | 26.70 | <0.001 | 36.20 | <0.001 | 37.30 | <0.001 |
| Isoleucine (μM) | 4.40 | 9.65 | 0.001 | 13.05 | <0.001 | 15.05 | <0.001 |
| Leucine (μM) | 11.40 | 29.20 | <0.001 | 43.50 | <0.001 | 56.80 | <0.001 |
| Phenylalanine (μM) | 9.40 | 26.45 | <0.001 | 43.25 | <0.001 | 44.45 | <0.001 |
| Tryptophan (μM) | 1.90 | 9.70 | <0.001 | 13.60 | <0.001 | 12.85 | <0.001 |
Differences in CSF amino acid level at day 0-3, 5, and 10 post-SAH in healthy individuals (control group) and SAH patients (study group).
Median levels of the amino acids are presented in the table. P-values were calculated either by Mann–Whitney or t-Student test. Amino acids increasing significantly during SAH (on day 0–3, 5, or 10) are shown in bold.
Table 3
| Monitored parameter | Day 0–3 post-SAH | Day 5 post-SAH | Day 10 post-SAH | ||||||
|---|---|---|---|---|---|---|---|---|---|
| GO-SAH | PO-SAH | GO-SAH vs. PO-SAH. P-value | GO-SAH | PO-SAH | GO-SAH vs. PO-SAH. P-value | GO-SAH | PO-SAH | GO-SAH vs. PO-SAH. P-value | |
| C-reactive protein (mg/l) | 125.00 | 130.00 | 0.715 | 50.70 | 115.80 | 0.020 | 22.40 | 109.70 | 0.139 |
| White blood cell count (106/mm3) | 9.86 | 17.47 | <0.001 | 10.57 | 15.03 | 0.044 | 9.66 | 16.84 | 0.445 |
| Haemoglobin (g/dl) | 12.10 | 13.10 | 0.212 | 11.30 | 10.90 | 0.262 | 10.60 | 11.50 | 0.672 |
| Temperature (OC) | 37.00 | 37.00 | 0.736 | 37.40 | 37.20 | 0.273 | 36.90 | 35.70 | 0.512 |
| Fibrinogen (mg/dl) | 485.00 | 550.00 | 0.879 | 509.00 | 676.00 | 0.257 | 428.00 | 698.00 | 0.014 |
| O-phosphoethanolamine (μM) | 4.10 | 6.80 | 0.161 | 4.90 | 7.70 | 0.161 | 5.95 | 8.50 | 0.019 |
| Taurine (μM) | 13.10 | 19.40 | 0.038 | 14.60 | 13.40 | 0.514 | 12.20 | 7.35 | 0.138 |
| Asparagine (μM) | 9.00 | 11.50 | 0.256 | 30.20 | 12.50 | 0.397 | 22.90 | 20.45 | 0.423 |
| Serine (μM) | 45.10 | 76.50 | 0.161 | 77.00 | 61.40 | 0.947 | 79.50 | 94.75 | 0.503 |
| Glycine (μM) | 29.60 | 41.00 | 0.182 | 30.00 | 43.00 | 0.204 | 35.20 | 35.65 | 0.671 |
| Hydroxyproline (μM) | 0.70 | 1.30 | 0.066 | 1.70 | 0.80 | 0.518 | 1.35 | 1.10 | 0.793 |
| Ethylaminev (μM) | 5.80 | 15.80 | 0.102 | 7.90 | 12.10 | 0.134 | 8.85 | 14.50 | 0.35 |
| Glutamine (μM) | 489.90 | 602.50 | 0.182 | 837.30 | 505.10 | 0.585 | 852.50 | 715.60 | 0.483 |
| Aspartic acid (μM) | 0.80 | 2.20 | 0.038 | 0.40 | 1.00 | 0.071 | 0.95 | 1.30 | 0.551 |
| Citruline (μM) | 1.90 | 5.90 | 0.035 | 2.50 | 3.10 | 0.396 | 3.30 | 3.30 | 1 |
| Threonine (μM) | 25.90 | 50.00 | 0.35 | 86.30 | 34.20 | 0.327 | 63.40 | 57.95 | 0.298 |
| Beta-Alanine (μM) | 28.30 | 22.00 | 0.07 | 30.00 | 28.60 | 0.497 | 26.85 | 27.85 | 0.893 |
| Alanine (μM) | 70.70 | 138.10 | 0.109 | 115.50 | 113.60 | 0.447 | 120.30 | 141.70 | 0.954 |
| Glutamic acid (μM) | (μM)3.40 | 9.30 | 0.038 | 2.70 | 5.80 | 0.024 | 2.00 | 17.10 | 0.269 |
| Histidine (μM) | 18.90 | 38.10 | 0.256 | 63.60 | 23.70 | 0.711 | 43.55 | 37.45 | 0.753 |
| 3-Methylhistidine (μM) | 0.70 | 1.50 | <0.01 | 1.80 | 1.20 | 0.958 | 1.35 | 1.05 | 0.733 |
| 2-Aminoadipic acid (μM) | 1.30 | 1.70 | 0.076 | 1.50 | 4.10 | 0.071 | 1.30 | 2.45 | 0.033 |
| Gamma-aminobutyric acid (μM) | 0.30 | 0.90 | 0.043 | 0.30 | 0.30 | 0.932 | 0.35 | 0.15 | 0.266 |
| 3-Aminoisobutyric acid (μM) | 0.20 | 0.40 | 0.066 | 0.30 | 0.30 | 0.958 | 0.35 | 0.45 | 0.93 |
| 2-Aminobutyric acid | 4.60 | 8.20 | 0.088 | 11.40 | 5.50 | 0.542 | 10.75 | 6.50 | 0.124 |
| Arginine (μM) | 18.50 | 22.30 | 0.182 | 30.90 | 26.00 | 0.525 | 24.35 | 20.45 | 0.21 |
| Proline (μM) | 4.50 | 14.80 | 0.204 | 18.70 | 10.90 | 0.672 | 16.15 | 18.40 | 0.759 |
| Ornithine (μM) | 7.60 | 27.50 | 0.033 | 17.30 | 28.30 | 0.09 | 17.35 | 33.05 | 0.552 |
| Cystathionine (μM) | 0.80 | 2.80 | <0.01 | 1.00 | 1.80 | 0.089 | 1.30 | 1.50 | 0.199 |
| Cysteine (μM) | 1.60 | 2.30 | 0.141 | 1.50 | 2.20 | 0.243 | 1.95 | 1.40 | 0.329 |
| Lysine (μM) | 43.00 | 74.50 | 0.095 | 87.10 | 55.90 | 0.341 | 72.40 | 72.60 | 0.612 |
| Methionine (μM) | 4.60 | 12.30 | 0.204 | 16.20 | 7.70 | 0.491 | 13.65 | 13.70 | 0.687 |
| Valine (μM) | 31.80 | 62.40 | 0.109 | 102.50 | 33.60 | 0.397 | 87.75 | 72.20 | 0.377 |
| Tyrosine (μM) | 23.00 | 32.50 | 0.083 | 36.60 | 35.80 | 0.876 | 38.60 | 37.30 | 0.676 |
| Isoleucine (μM) | 6.00 | 13.00 | 0.045 | 17.60 | 11.80 | 0.597 | 15.05 | 13.70 | 0.45 |
| Leucine (μM) | 22.90 | 39.70 | 0.109 | 80.30 | 29.80 | 0.397 | 60.95 | 50.55 | 0.532 |
| Phenylalanine (μM) | 21.20 | 38.80 | 0.062 | 51.30 | 32.40 | 0.665 | 45.40 | 44.45 | 0.913 |
| Tryptophan (μM) | 8.00 | 12.20 | 0.066 | 13.60 | 13.60 | 0.606 | 12.60 | 13.55 | 0.804 |
Differences in monitored parameters at day 0-3, 5, and 10 post-SAH in patients with good (GO-SAH) and poor (PO-SAH) treatment outcome.
Median levels of the amino acids are presented in the table. P-values were calculated either by Mann–Whitney or t-Student test. Significant differences are shown in bold.
Table 4
| Parameter | Day 0–3 post-SAH | Day 5 post-SAH | Day 10 post-SAH | Omnibus test P-value | Day 0–3 vs. Day 5 P-value Post-hoc | Day 0–3 vs. Day 10 P-value Post-hoc | Day 5 vs. Day 10 P-value Post-hoc |
|---|---|---|---|---|---|---|---|
| C-reactive protein (mg/l) | 125.00 | 50.70 | 27.00 | 0.049 | 0.345 | 0.012 | 0.073 |
| White blood cell count (106/mm3) | 9.86 | 10.57 | 9.34 | 0.631 | |||
| Haemoglobin (g/dl) | 12.10 | 10.60 | 10.50 | 0.066 | |||
| Temperature (OC) | 37.00 | 37.30 | 36.90 | 0.382 | |||
| Fibrinogen (mg/dl) | 485.00 | 442.00 | 423.00 | 0.327 | |||
| O-phosphoethanolamine (μM) | 6.20 | 5.00 | 5.80 | 0.325 | |||
| Taurine (μM) | 13.90 | 14.60 | 10.80 | 0.867 | |||
| Asparagine (μM) | 8.40 | 18.30 | 21.20 | 0.180 | |||
| Serine (μM) | 45.10 | 73.80 | 76.10 | 0.031 | 0.039 | 0.013 | 0.566 |
| Glycine (μM) | 29.60 | 32.60 | 42.50 | 0.565 | |||
| Hydroxyproline (μM) | 0.70 | 1.10 | 1.40 | 0.094 | |||
| Ethylaminev (μM) | 5.70 | 10.10 | 9.80 | 0.039 | 0.078 | <0.01 | 0.187 |
| Glutamine (μM) | 454.30 | 818.70 | 828.30 | 0.001 | <0.01 | <0.001 | 0.565 |
| Aspartic acid (μM) | 0.70 | 0.40 | 1.20 | 0.215 | |||
| Citruline (μM) | 1.90 | 2.40 | 2.80 | 0.069 | |||
| Threonine (μM) | 22.90 | 52.50 | 61.20 | 0.012 | 0.128 | <0.01 | <0.01 |
| Beta-Alanine (μM) | 28.30 | 26.80 | 26.70 | 0.553 | |||
| Alanine (μM) | 70.70 | 106.20 | 120.30 | 0.124 | |||
| Glutamic acid (μM) | 4.50 | 2.70 | 2.10 | 0.898 | |||
| Histidine (μM) | 16.80 | 38.10 | 37.40 | 0.156 | |||
| 3-Methylhistidine (μM) | 0.60 | 1.50 | 0.90 | 0.369 | |||
| 2-Aminoadipic acid (μM) | 1.30 | 1.50 | 1.30 | 0.129 | |||
| Gamma-aminobutyric acid (μM) | 0.30 | 0.20 | 0.50 | 0.633 | |||
| 3-Aminoisobutyric acid (μM) | 0.20 | 0.30 | 0.30 | 0.215 | |||
| 2-Aminobutyric acid (μM) | 4.60 | 11.40 | 11.40 | 0.010 | 0.019 | <0.01 | 0.427 |
| Arginine (μM) | 18.50 | 26.50 | 24.10 | 0.050 | 0.073 | 0.012 | 0.345 |
| Proline (μM) | 3.40 | 11.90 | 15.20 | 0.062 | |||
| Ornithine (μM) | 7.60 | 17.30 | 14.30 | 0.368 | |||
| Cystathionine (μM) | 1.00 | 1.00 | 1.20 | 0.648 | |||
| Cysteine (μM) | 1.60 | 1.70 | 2.10 | 0.368 | |||
| Lysine (μM) | 43.00 | 83.20 | 70.80 | 0.017 | 0.017 | <0.01 | 0.735 |
| Methionine (μM) | 4.60 | 10.00 | 11.90 | 0.008 | 0.029 | <0.01 | 0.200 |
| Valine (μM) | 28.20 | 61.60 | 78.10 | 0.015 | 0.017 | <0.01 | 0.683 |
| Tyrosine (μM) | 23.00 | 35.10 | 28.50 | 0.020 | 0.023 | <0.01 | 0.647 |
| Isoleucine (μM) | 5.90 | 11.10 | 13.60 | 0.024 | 0.081 | <0.01 | 0.219 |
| Leucine (μM) | 22.60 | 39.70 | 60.50 | 0.024 | 0.022 | 0.013 | 0.801 |
| Phenylalanine (μM) | 20.90 | 35.20 | 37.50 | 0.038 | 0.040 | 0.018 | 0.700 |
| Tryptophan (μM) | 8.00 | 11.80 | 11.10 | 0.072 |
Amino acid level changes in time in good outcome SAH patients with (GO-SAH).
Median levels of the amino acids are presented in the table. Omnibus statistical test (Friedman or repeated measures ANOVA) revealed significant changes in concentration of 13 amino acids (shown in bold). Next, post-hoc analysis (Conover–Iman for Friedman test and Fisher for repeated measures ANOVA) was run for those 13 amino acids to specify between what time points (day 0–3, 5, or 10 post-SAH) the differences in concentration were significant (shown in bold).
Figure 1
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Figure 4
Statements
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Summary
Keywords
subarachnoid haemorrhage, amino acids, early brain injury, delayed cerebral ischaemia, biomarkers
Citation
Sokół B, Urbaniak B, Wąsik N, Plewa S, Klupczyńska A, Jankowski R, Więckowska B, Juszkat R and Kokot Z (2018) Corrigendum: Amino Acids in Cerebrospinal Fluid of Patients With Aneurysmal Subarachnoid Haemorrhage: An Observational Study. Front. Neurol. 9:416. doi: 10.3389/fneur.2018.00416
Received
03 May 2018
Accepted
22 May 2018
Published
13 June 2018
Volume
9 - 2018
Edited and reviewed by
J. Marc Simard, University of Maryland, Baltimore, United States
Updates
Copyright
© 2018 Sokół, Urbaniak, Wąsik, Plewa, Klupczyńska, Jankowski, Więckowska, Juszkat and Kokot.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Bartosz Sokół bartosz.sokol@ump.edu.pl
This article was submitted to Neurocritical and Neurohospitalist Care, a section of the journal Frontiers in Neurology
Disclaimer
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