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Original Research ARTICLE

Front. Oncol., 16 July 2020 | https://doi.org/10.3389/fonc.2020.01103

Spectrum of TP53 Mutations in BRCA1/2 Associated High-Grade Serous Ovarian Cancer

Ulyana A. Boyarskikh1*, L. F. Gulyaeva2,3, A. M. Avdalyan4, A. A. Kechin1, E. A. Khrapov1, D. G. Lazareva5, N. E. Kushlinskii6, A. Melkonyan7, A. Arakelyan7 and Maxim Leonidovich Filipenko1
  • 1Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences (SB RAS), Novosibirsk, Russia
  • 2Institute for Medicine and Psychology, Novosibirsk State University, Novosibirsk, Russia
  • 3Institute of Molecular Biology and Biophysics, Federal Research Center of Fundamental and Translational Medicine, Novosibirsk, Russia
  • 4City Clinical Hospital No. 40, Moscow, Russia
  • 5Altai Territorial Cancer Control Center, Barnaul, Russia
  • 6N.N. Blokhin National Medical Research Center of Oncology, Moscow, Russia
  • 7Group of Bioinformatics, Institute of Molecular Biology, Armenian National Academy of Sciences (NAS RA), Yerevan, Armenia

Objective: Mutations in TP53 lead to loss of function (LOF) or gain of function (GOF) of the corresponding protein p53 and produce a different effect on the tumor. Our goal was to determine the spectrum of somatic TP53 variants in BRCA1/2 associated high-grade serous ovarian cancer (HGSOC).

Methods: The population under study comprised of HGSOCs with pathogenic variants in BRCA1 (n = 78) or BRCA2 (n = 21). Only chemo-naive and platinum-sensitive patients were included in this study. The case group of the IARC database (n = 1249) with HGSOC not stratified by BRCA status was used as a reference. A custom NGS panel was used for sequencing TP53 and mutational hot-spots of other genes, and p53 expression was evaluated by immunohistochemistry for 68 cases of HGSOCs.

Results: Somatic TP53 variants (95) or inhibition of wild-type p53 expression (3) were observed in 98 cases. The sample with normal p53 had CDKNA1 variants. The frequency of truncating variants was significantly higher than in the reference cohort (30.3 vs. 21.0%, p = 0.01). Most of the samples (41/68) demonstrated low (or absent) expression of p53, and 17 samples overexpressed p53. LOH was typical for TP53 nonsense variants (14/15). In total, 68/95 samples were LOH positive and showed LOH in all tumorous cells, thus indicating the driver effect of TP53 mutations. Three specimens had KRAS, BAX, APC, and CTNNB1 subclones variants.

Conclusion: High frequency of TP53 truncating variants, the low expression of mutant p53, and low incidence of oncogene mutations show potential GOF properties of p53 to be poorly represented in BRCA1/2 associated HGSOC.

Introduction

TP53 is the most frequently mutated gene in human cancer (1). About 75% of TP53 variants are missense substitutions (2). Other alterations include frameshift indels and nonsense variants (20%) and infrequent substitutions in splicing sites, inframe indels, and silence variants. Frameshift indels and nonsense variants always lead to the null p53 phenotype. In contrast, missense variants result in a full-size mutant p53, which can stably express and have a different effect on the tumor. The loss or decrease in p53 transcriptional activity (loss of function, LOF) is a common property of all p53 mutants associated with cancer. Along with LOF, a number of additional p53 action scenarios are possible. Specifically, variants of “separation of functions” are described (3). In this case, the mutant retains some pro-survival functions and selectively loses tumor-suppressive activity of p53 wild-type, as shown for the apoptosis-deficient R175P mutant p53 (4). Finally, some mutants acquire new oncogenic properties (gain of function, GOF). Studies of cancer cell lines and animal cancer models have shown that GOF TP53 variants can contribute to chemotherapy resistance and cancer progression (3, 58). Thus, mutant p53 plays a complex role in tumorigenesis that varies depending on both the mutation type and tumor origin.

The prevalence of missense variants and the fact that GOF mutants of TP53 promote tumor progression led to speculation about the positive selection of GOF variants during carcinogenesis.

High-grade serous ovarian carcinoma (HGSOC) is the most common type of ovarian cancer characterized by difficulties in early detection and high mortality rates. HGSOC is a unique type of cancer in terms of the prevalence of TP53 mutations. Almost all HGSOC tumors (95%) carry somatic TP53 variants. A comparable incidence of TP53 mutations is observed only in serous endometrial carcinomas (89%) and basal subtype breast tumors (88%) (1, 9). Almost 20% of HGSOCs are associated with germline BRCA1/2 variants (10). BRCA proteins is involved in the repair of DNA double-strand breaks by homologous recombination (HR) (11). The complete loss of BRCA1/2 function leads to disruption of the HR-based DNA repair and, as a result, to the large-scale genomic instability (12). In this case, alterations in TP53 (or other cell cycle control genes) are mandatory for the cell viability. Otherwise, genomic instability results in cell-cycle arrest and apoptosis. For HGSOC with an HR deficiency, we assumed LOF of p53 to be selected more frequently than GOF. To test this hypothesis, we determined the frequencies of various types of TP53 variants in the cohort of BRCA1/2-deficient HGSOCs. To evaluate the involvement of other genes in BRCA1/2-associated HGSOC pathogenesis, we tested these tumors for the frequent somatic variants in oncogenes.

Materials and Methods

Patients and Samples

Samples were obtained from patients with relapse of high-grade serous ovarian adenocarcinomas after first-line treatment, undergoing tumor testing under the program “Improving the system of molecular genetic diagnosis of cancer in the Russian Federation” (http://www.cancergenome.ru). Ethical approval for this project was obtained from the Institute of Molecular Biology and Biophysics Ethics Committee (Protocol: # 1 dated March 14, 2017). Written informed consent was obtained from all subjects.

All patients underwent primary cytoreductive surgery, followed by adjuvant platinum-based chemotherapy as first-line treatment. Eligibility criteria for the study were (1) histologically verified diagnosis of high-grade serous ovarian (or fallopian tube) adenocarcinomas; (2) lack of neo-adjuvant therapy; (3) platinum sensitivity (the time from adjuvant platinum-based treatment to cancer relapse (platinum-free interval, PFI) was > 6 months), (4) available blood sample and FFPE tissue from the primary tumor; and (5) germline pathogenic variants in BRCA1 or BRCA2. The FFPE primary tumor blocks were sectioned and stained with hematoxylin and eosin (H&E). Tumor regions on H&E stained slides were marked, and the percentage of tumor cells was estimated. Samples containing at least 10% of tumor cells were selected for this study. DNA was extracted from marked regions separated by manual macrodissection from three unstained 10 μm-thick FFPE sections. DNA isolation was carried out using alkaline lysis followed by DNA extraction from the precipitate, as described previously (13). DNA from blood leukocytes was extracted using an in-house method involving cell lysis using 10% SDS-containing buffer, proteinase K treatment, protein extraction using phenol-chloroform, and isopropanol precipitation of the DNA. All DNA samples were screened for sufficient quantity using the PCR based QC Kit (Kapa Biosystems). Germline BRCA1/2 variants were determined by NGS of gene coding sequences and splicing site regions, as described previously (14). DNA samples from both leukocyte and FFPE tumors were sequenced. As a result, 99 tumors were revealed, with 78 being BRCA1 germline mutation carriers and 21 – BRCA2 (the full list of the BRCA variants is given in the Supplementary Table 1).

NGS Panel and Data Analysis

DNA target sequencing was performed using the PCR-based custom NGS panel called CCMSeq (Common Cancer Mutations). CCMSeq panel was designed for analyzing multiple genome regions that are commonly mutated in a variety of cancer types. This panel covers 8.6 kilobases across all 11 exons and adjacent intron regions of TP53, as well as coding regions of cancer-related genes that carry the most frequent somatic variants. These regions were selected based on the whole genome (or exome) sequencing data cataloged in the COSMIC database for colon, stomach, lung, breast, and ovary cancers. Regions carrying somatic mutations with a prevalence of at least 2% in two or more cancer types were included in the CCMSeq panel. In total, loci of 50 genes were selected for the CCMSeq panel (Supplementary Table 2). These selected targets were amplified using two multiplex PCRs with the amplicon library preparation procedure described previously (14). Normalized amplicon libraries were sequenced on a MiniSeq platform (Illumina) using a MiniSeq High Output Reagent Kit (300 cycles).

The procedure of NGS data analysis was similar to those described previously (14) with some modification: short-variant calling was performed by Pisces (https://github.com/Illumina/Pisces, somatic mode). To filter the false positive variants, those with less than six reads of alternative alleles were discarded. The coverage (median coverage of one library amplicon) ranged from 163 to 7273 reads, with median and Q1-Q3 values of 1087 and 735-2037 reads, respectively. The following variant types were taken into consideration: (1) frameshift, stop gained, stop lost, start lost, splice acceptor, splice donor variants; (2) missense and splice region variants that according to 1000Genomes project have a population frequency less than 0.5%; (3) variants listed in the COSMIC database; (4) variants registered in the ClinVar database as “Pathogenic”/”Likely pathogenic.” Differentiation between somatic and germline variants was performed by sequencing both leukocyte and tumor DNA samples. The variants with low variant allele frequency (VAF) were filtered out. The cut-off of VAF was 5% - for tumor DNA and 20% - for leukocyte DNA. DNA samples with somatic variant VAF <15% were sequenced twice.

The NGS data supporting this study have been deposited in the National Center for Biotechnology Information (NCBI)'s Sequence Read Archive (SRA, https://www.ncbi.nlm.nih.gov/sra) with BioProject ID PRJNA612603 and can be accessed at https://www.ncbi.nlm.nih.gov/sra/PRJNA612603.

Classification of TP53 Variants

TP53 variants were classified into three categories: GOF, LOF, and “unclassified,” according to the criteria described by Brachova et al. (15). Specifically, TP53 variants were defined as GOFs, based on experimental studies that showed the oncogenic properties of mutant p53. Eight TP53 mutations were considered as GOF: P151S, Y163C, R175H, L194R, Y220C, R248Q/W, R273C/H/L, R282W. Nonsense and frameshift variants leading to significant disruptions in the p53 translation were classified as LOF. The remaining missense and splice site variants, the function of which is not yet well known, were categorized as “unclassified” variants.

Additionally, we used the International Agency for Research on Cancer (IARC) TP53 database to further characterize all missense variants by the transcriptional and GOF activity in corresponding mutants (http://p53.iarc.fr/DownloadDataset.aspx (Files: somatic Mutation Data IARC TP53 Database, R20.txt, and functional Assessment IARC TP53 Database, R20.txt)). Characterization of GOF missense variants was performed, as described previously (16). As a reference group of variants specific for HGSOC the variant set of the IARC TP53 database (File: somatic Variant Data IARC TP53 Database, R20.txt) non-stratified by BRCA status, was used. Only the cases with morphology corresponding to adenocarcinoma and cystadenocarcinoma (1249 in total) were selected. Ethnicity and BRCA status were not indicated for most samples. The reference set of TP53 variants did not contain silent variants.

Pathomorphological and Immunohistochemical Assessment

The percentage of tumor cells relative to other cells was estimated independently by two pathologists using the same slides stained with hematoxylin and eosin. In six tumors with extensive inflammatory infiltration and/or diffuse stromal invasion, epithelial cells were labeled with a pan-cytokeratin antibody cocktail (antibody clone AE1/AE3, M3515, Dako, CA, USA) for a more accurate estimation of tumor cell percentage. Finally, the percentage of tumor cells was calculated as the average value of both measurements. The mean difference between the measurements was 6 ± 4%. The percentage of tumor cells ranged from 10 to 95% with the median and Q1-Q3 values of 60% and 45–75%, respectively (the actual percentage of tumor cells is given in Supplementary Table 1). FFPE tissue sections were subject to immunohistochemistry for p53 using a commercially available mouse monoclonal anti-human p53 antibody (clone DO-7, M7001, Dako, CA, USA) at the dilution of 1:50. Staining was performed on a whole section using a Ventana BenchMark GX autostainer (Roche) according to the standard protocol in automatic mode. Stained slides were examined by an experienced surgical pathologist who was not aware of molecular data. The percentage of cells with positive nuclear staining was estimated and subdivided into three categories: ≥ 70% positively stained nuclei (High); >10% and < 70% stained nuclei (Intermediate); ≤ 10% positively stained nuclei (Low).

Statistical Methods

Statistical analyses were performed applying R 2.13.1 statistical software. Results were compared using the χ2 test.

Results

Patient Characteristics

Ninety nine patients with HGSOC (4 with fallopian tube carcinoma and 95 with ovarian carcinoma) were enrolled in this study. In most patients (67/99, 67.0%), ovarian cancer was diagnosed at the age > 50 years. The median and Q1-Q3 values of age were 54 (49-60) years. The cohort of patients with HGSOC comprised cases with II (15), (III) 71, and IV (13) FIGO stages. Tumor grade was determined as G2 in 19 cases, G3 in 73 cases, and undetermined for others cases. All 99 patients underwent primary cytoreductive surgery, followed by first-line platinum-based chemotherapy, most with carboplatin-paclitaxel regimen (88). Other treatments included cisplatin-doxorubicin-cyclophosphamide (5), or not specified (platinum-based) (6). All patients experienced a complete or partial response after adjuvant therapy with platinum-free interval (PFI) > 6 months. For 32/99 patients, PFD was specified and ranged from 7 up to 21 months (median (Q1-Q3), 13 (11-16) months). All patients were carriers of a germline pathogenic BRCA1 or BRCA2 variants (78 and 21 cases, respectively). BRCA1 variants (5382insC, 300T/G, 4153delA, 2080delA, 3819del5, and 3875del4), highly prevalent in Slavs, accounted for 47.4% of total; full list of the BRCA variants is given in the Supplementary Table 1.

Repertoire and Distribution of Somatic Variants in HGSOC

FFPE primary tumors from 99 patients with relapse of BRCA-deficient ovarian cancer and paired blood samples were sequenced using CCMSeq panel. The detected somatic variants were absent in paired blood samples. Only somatic variants affecting the amino acid sequence of a protein were considered. A total of 106 somatic variants were identified across six genes (TP53, APC, BAX, KRAS, CDKN2A, CTNNB1) in 96/99 patients (see the variants destination and ClinVar classification in the Supplementary Table 1).

TP53 variants were found in 95 patients with HGSOC. In two cases, the tumor cells carried two TP53 variants. TP53 variants were grouped into three categories: GOF (22), LOF (30), and “unclassified” (45) (Figure 1, Table 1). We compared the results with the distribution of GOF, LOF and “unclassified” variants in the HGSOC variant set of the IARC TP53 database. The frequency of LOF variants (261/1249, 21%) in this group was significantly lower (p < 0.01).

FIGURE 1
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Figure 1. Somatic mutations distribution in BRCA1/2 associated HGSOC (n = 99). VAF*, normalized variant allele frequency of mutant TP53 allele; S, variants of donor/acceptor splice sites; F, frame shift mutations; N, nonsense mutations; 1, BRCA1 mutations; 2, BRCA2 mutations.

TABLE 1
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Table 1. Distribution of various types of somatic mutations.

There were no significant differences between the distribution of various types of TP53 variants in BRCA1- and BRCA2-deficient HGSOC. Some of the “unclassified” TP53 variants (16/45) were annotated in the ClinVar database as pathogenic, the functional significance of the remaining variants unknown. Most of the “unclassified” variants (38/45) were amino acid substitutions located in the DNA-binding domain (DBD, 34 variants), C-terminal tetramerization domain (2 variants) or interdomain spacer (residue 93 between Transactivation Domain and DBD, 2 variants). The rest of the “unclassified” variants were those of donor/acceptor splice sites (3), of splice regions (3) and disruptive inframe deletion (1).

The majority of variants (58/97) identified in BRCA1/2 associated HGSOC were “unclassified” and GOF missense. We used the IARC database information to further characterize all missense variants by the presence of transcriptional and GOF activity in the corresponding mutants. In respect to transcriptional activity, the common missense variants (54/58, 93.1%) were classified as “nonfunctional,” with the rest being “partially functional” (3) and “functional” (1). Mutants with preserved transcriptional activity were from the “unclassified” group. For most TP53 variants, there were no data on their GOF properties. Petitjean et al. systematized information from the IARC database on GOF properties of 103 TP53 mutants (16). The authors identified three categories of GOF activity: (1) interference with p73, (2) transactivation of genes repressed by wild-type p53, and (3) cooperation with oncogenes for the transformation of mouse embryonic fibroblast cells. In our study, a variant was classified as GOF if the corresponding mutant had at least one of the GOF activities. According to these criteria, 6 of 43 “unclassified” missense variants were determined as GOF.

Six samples had nine concomitant variants in non-TP53 genes such as APC (1), BAX (1), KRAS (1), CDKN2A (4), CTNNB1 (2) (Figure 1, Supplementary Table 1). There was no significant association between the occurrence of these mutations and the clinical outcome of the corresponding patients, possibly due to the small size of this patient group.

Loss of Heterozygosity of TP53

For samples carrying somatic variants, we determined the percentage of tumor cells with loss of heterozygosity (LOH). To this end, for each mutation, the variant allele frequency (VAF) was calculated as the ratio of the variant allele reads number to the total number of reads. The tumor content estimated by histological sections assay was used to normalize VAF as :

Normalized VAF = VAF% Tumor cells

The number of variant allele reads is (1+K) × T, where T is the number of tumor cells in the sample, and k is the proportion of cells with a LOH, so that

Normalized VAF = (1+k)2

For all HGSOC samples with one exception, normalized VAFs were either 0.50 ± 0.05 (29/94) or 1.00 ± 0.08 (65/94), which corresponds to the proportion of tumor cells with LOH, k = 0 or k = 100% (Figure 2). For one sample carrying a single nucleotide deletion in TP53, VAF was 1.6, most likely caused by a deviation in the ratio of amplified alleles at the library preparation stage. We found the rate of the tumors with the LOH-positive variant of TP53 to be about 71% (68/95). Most of the nonsense TP53 variants (14/15, 93%) were LOH-positive. Among other types of variants, the LOH frequency ranged from 60 to 70% (Figure 1).

FIGURE 2
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Figure 2. Normalized variant allele frequency (normalized VAF) for somatic mutation in BRCA1/2 associated HGSOC. Normalized VAFs of 0.5 or 1 correspond to a percentage of LOH positive tumor cells of 0 or 100%. Normalized VAFs of <0.5 indicate that the mutation is contained in the subclones of the tumor; the LOH status for these mutations is not determinable.

We did not observe intermediate values 0 < k < 1 for TP53 variants, while for 3/9 cases in the APC (1), KRAS (1), CDKN2A (1) genes, the normalized VAF was 0.23–0.37, indicating that only subclones of tumor cells carry these somatic variants.

Immunohistochemical Assay of p53

There is a common agreement that both types of abnormal p53 expression (high and low, or absent) are correlated with mutant p53. The expression level is usually determined by the content of p53 positively stained nuclei. In various studies of HGSOC, the cut-off for high p53 expression levels indicating mutation ranged from 50 to 85% (1719). In the present study, the cut-off was 70%, as described by Cole et al. (20). We carried out p53 immunohistochemistry for 68/99 patients, with 56 cases (82.3%) found to have abnormal p53 expression (Table 2 and Supplementary Table 1). In addition, we searched for differences in p53 protein levels between patients with GOF, LOF, and “unclassified” variants. Most of the analyzed samples with GOF variants (11/14) were p53 high-positive, while all analyzed tumors carrying LOF variants (9) were low-positive or negative. For the “unclassified” mutants, abnormal p53 expression was found in 31 of 40 cases only. The rest of the “unclassified” tumors had normal (intermediate) p53 expression with the actual number of nuclei staining positive for p53 in the “intermediate” category ranging from 30 to 56% (Supplementary Table 1). For the samples with wild-type TP53, only one had normal expression (65% of positive nuclei), while three showed low expression or absence of p53.

TABLE 2
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Table 2. Immunohistochemical classification p53 status for tumor samples with various types of somatic TP53 mutations.

Discussion

The frequency and spectrum of TP53 variants are highly variable and depend on the type of cancer (2). It is not yet clear what spectrum of TP53 variants is specific for BRCA1/2 associated HGSOC. In our study, we first examined the frequency of various types of TP53 variants in HGSOC patients with a germline BRCA1 or BRCA2 pathogenic variants.

Recently, conflicting evidence has emerged on the association of mutated TP53 type with platinum treatment resistance (2124). To exclude the possible influence of this factor, only chemo-naive and chemo-sensitive patients with PFI > 6 months were taken in this study.

Our results showed that the frequency of true LOF variants leading to the truncated protein among BRCA1/BRCA2 carriers with HGSOC was significantly higher than in reference cohort with HGSOC not stratified by BRCA status and chemo-sensitivity (30.3 vs. 21.0%, p = 0.01). This finding overlaps with the results of the study by Dumay et al. (25), in which basal-like breast tumors displayed significantly more truncating variants than luminal tumors (43 vs. 25%). Breast cancer with BRCA1 (but not BRCA2) mutations is known to typically have a basal phenotype. Apparently, there is a causal relationship between an increase in the frequency of truncating variants and BRCA1 alteration.

Truncating variants usually result in loss of any activity of wild-type p53. In contrast, functional outcomes of TP53 missense variants can be very diverse: LOF, acquisition of oncomorphic function (GOF), or no effect. According to IARC annotation, in our study, the majority (93%) of the missense mutants lacked transactivation activity (26), whereas GOF was described for 28 mutants (22, with conventional GOF variants and 6, with “unclassified” variants) (16). However, it should be kept in mind that most experimental studies of GOF were focused on testing the frequent TP53 variants clustered at codons 175, 245, 248, 249, 273, and 282. For non-hot spot TP53 variants, there are insufficient data on their GOF properties, making it impossible to perform a system analysis of GOF for all TP53 variants.

We used the expression level of the TP53 gene as an indirect marker of mutant p53 with GOF properties, as reviewed by other authors (27, 28). It is conceivable that mutant p53 accumulation in tumors is crucial to exert its GOF in carcinogenesis (2932). To characterize TP53 variants with respect to p53 protein expression, we performed IHC with 68 samples. Most of the analyzed samples with GOF variants (11/14) were p53 high-positive, while all analyzed tumors carrying LOF variants (9) were p53 low-positive or negative (Table 2). These results suggest the absence or low level of expression to predict a loss of p53 function, with a sensitivity of 100% and a specificity of 73%. According to this criterion, 25 of 40 IHC tested “unclassified” missense variants with the low and absent level of p53 expression can be defined as probable LOF. Importantly, only 6/40 “unclassified” mutants were p53 high-positive, and the rest (9) had an intermediate level of protein expression. If an elevated expression of mutant p53 is a factor of pro-oncogenic activity, then 9 variants in the samples with a normal level of p53 expression cannot be classified as GOF.

Four BRCA-deficient HGSOC (4/99) did not have somatic variants in TP53, but, surprisingly, 3/4 “normal” samples did show abnormal (low or absent) p53 expression levels, i.e., three of four tumors lost p53 function. A possible reason for the loss of p53 function is the deregulation of p53 stability, for example, through the amplification of MDM2, the protein of which regulates p53 proteasome degradation (33).

An important feature of p53 function is the integrity of the second TP53 allele. Most often, inactivation of the second allele occurs through copy-neutral LOH. In our study, LOH was determined based on the normalized VAF. Interestingly, for all LOH-negative and LOH-positive samples, the proportion of tumor cells with LOH was close to 0 and 100%, respectively (with one exception). Based on these findings, we hypothesized that there are no significant sub-clonal populations of cells with different LOH-status of TP53 in primary BRCA1/2-associated HGSOC. This assumption is consistent with the driver role of p53 in carcinogenesis. Thus, potential intratumor heterogeneity and clonal evolution under the pressure of treatment or metastasis will result from the selection of concomitant non-TP53 somatic variants like BRCA1/2 and other genes.

Since LOH is a sign of driver variant, it can be assumed that the proportion of LOH-positive variants of TP53 will be close to 100%. It was the case for tumors with nonsense TP53 variants, where the incidence of LOH was 93% (14/15). In other cases, the rate of LOH-positive variants was about 67%. The relatively low frequency of LOH can be explained by the existence of an alternative way to disable the second TP53 allele through the interference of p53 missense mutants with wild-type p53 (dominant-negative effect, DNE) (34).

In addition to TP53, we sequenced the loci of genes containing frequent somatic variants specific to solid tumors, including ovarian cancer. Additional somatic variants were found in six patients. A total of 9 variants were found in the genes APC (1), BAX (1), KRAS (1), CDKN2A (4), CTNNB1 (2). Three out of the six patients (including one patient with normal p53) had variants in the CDKN2A gene whose protein products p14ARF and p16INK4a act as tumor suppressors due to negative regulation of the cell cycle (35).

Variants of BAX, coding core regulators of apoptosis and (or) APC and CTNNB1 were detected in three tumors. The products of both genes (APC and CTNNB1) are components of the Wnt-signaling pathway, an important element in the regulation of embryogenesis and cell differentiation. (36). Studies of cancer genetics showed that genes encoding proteins of the Wnt pathway are frequent targets for mutational alterations in various cancers, including colon, prostate, breast, and ovarian cancer (37). The aberrant activation of the APC/b-catenin pathway is suggested to be restricted to endometrioid ovarian cancer. It is possible that these two samples were incorrectly classified as adenocarcinoma or contain subclones with different histological differentiation.

Variants in other classical oncogenes specific for solid tumors were not detected except for the only specimen carrying the KRAS variant. Based on the normalized VAFs of TP53 and KRAS variants (0.51 and 0.23, correspondingly), two equally possible clonal architecture of the tumor can be suggested. First, the tumor contains clonal driver mutation of TP53 (without LOH) and subclonal (affecting about 50% of cells) mutation KRAS likely to have occurred later in tumor evolution. Second, mutations of TP53 (with LOH) and KRAS are independent subclones (each affecting about 50%). Perhaps the tumor is a mix of high and low-grade serous cancer (since the primary KRAS mutations are characteristic of low-grade ovarian carcinoma). Then, the question arises of the multiclonal origin of the tumor. According to the current concept of serous carcinoma pathogenesis, the second option seems less likely. However, it should not be excluded.

According to the latest data, the same variants of TP53 exert different properties depending on the origin, stage, and molecular profile of a tumor. It was previously shown that patients with HGSOC carrying concurrent somatic variants in TP53 and additional driver oncogenes had a worsened prognostic signature (reduced PFI, time to recurrence and OS) (18). On the other hand, it is known that the p53 mutants cooperate synergistically with other oncogenes (RAS, TGF-b), causing a more aggressive cancer (3842). In our study, most samples of HGSOC did not have additional (other than TP53 and BRCA1/2) variants in oncogenes, which seems to be a favorable prognostic factor. Apparently, under such microenvironment, potential GOF (pro-oncogenic) properties are less likely to manifest.

This study has potential limitations. (1) As a reference group, the set of variants specific for HGSOC from the IARC TP53 database was used. For most cases of the reference group, ethnicity and BRCA status were not defined. It is likely that some reference cases of HGSOC are associated with BRCA. A case-control study (BRCA1/2 associated vs. sporadic HGSOC) has more sensitivity to detect differences in the compared samples (would be more preferable). The source of bias is the probable ethnicity heterogeneity of the compared samples and, consequently, the heterogeneous structure of inherited genetic factors, for example, prevalence and spectrum of BRCA1/2 mutations. Therefore, our findings are supposed to be confirmed by studies with large samples adjusted to ethnicity. (2) To determine non-TP53 somatic mutations, a target panel was used that covers the loci most frequently mutated in solid cancers. However, relatively rare cancer genes have not been sequenced although it can be expected that some of them might have clinical relevance.

Conclusions

We have focused on molecular profiling of chemo-naive and platinum-sensitive HGSOC with germline BRCA1/2 variants. Using NGS we have analyzed both the set of TP53 variants and somatic variants of other genes involved in carcinogenesis. Our findings showed that somatic TP53 variant or inhibition of wild-type p53 expression was observed in almost 100% of cases with BRCA1/2 associated HGSOC. Rare exceptions are accompanied by variants in other genes of the cell cycle, confirming earlier observations that the negative regulation of cell cycle checkpoints is the main hallmark of BRCA-deficient class of HGSOC. With missense variants predominating among TP53, the proportion of truncating variants is significantly higher than with a mixed (in terms of BRCA mutations and sensitivity to platinum) cohort of HGSOC. LOH is typical for TP53 nonsense variants only, while for other types of variants, there is no pattern in the distribution of LOH. Loss of transcription activities is a common property of missense p53 mutants. There are several indirect signs (normal or low expression of mutants p53, low incidence of concomitant oncogenes mutations) indicating the low manifestation of the GOF properties of TP53 variants in BRCA1/2 associated HGSOC. Due to the driver role of TP53, its variants will persist in all tumorous subclones during treatment or metastasis. This knowledge can be useful in the management of patients with advanced ovarian cancer.

Data Availability Statement

The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found below: https://www.ncbi.nlm.nih.gov/sra/PRJNA612603, Accession no: PRJNA612603.

Ethics Statement

The studies involving human participants were reviewed and approved by the Institute of Molecular Biology and Biophysics Ethics Committee (Protocol: # 1 dated March 14, 2017). The patients/participants provided their written informed consent to participate in this study.

Author Contributions

UB and LG: conceptualization. UB: funding acquisition. DL, NK, and AMA: resources (patients and clinical description). EK, AMA, and NK: investigation. AK, AM, and AA: software. UB and AK: formal analysis. MF: supervision. UB and LG: writing—original draft. All authors: writing—review and editing.

Funding

The study was supported by the grants of Russian Foundation for Basic Research—(18-515-05004) and the Science Committee of the MESCS RA (18RF-112).

Conflict of Interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Supplementary Material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fonc.2020.01103/full#supplementary-material

Supplementary Table 1. List of BRCA1/2 germline mutations and somatic mutations in the HGSOC samples.

Supplementary Table 2. List of the custom NGS panel loci.

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Keywords: TP53 somatic mutations, p53 expression, gain of function, loss of function, BRCA1/2 carriers, ovarian cancer

Citation: Boyarskikh UA, Gulyaeva LF, Avdalyan AM, Kechin AA, Khrapov EA, Lazareva DG, Kushlinskii NE, Melkonyan A, Arakelyan A and Filipenko ML (2020) Spectrum of TP53 Mutations in BRCA1/2 Associated High-Grade Serous Ovarian Cancer. Front. Oncol. 10:1103. doi: 10.3389/fonc.2020.01103

Received: 01 April 2020; Accepted: 02 June 2020;
Published: 16 July 2020.

Edited by:

Marco Cordani, IMDEA Nanociencia, Spain

Reviewed by:

Alvaro Monteiro, Moffitt Cancer Center, United States
Giovanni Gaudino, Retired, Bellinzona, Switzerland

Copyright © 2020 Boyarskikh, Gulyaeva, Avdalyan, Kechin, Khrapov, Lazareva, Kushlinskii, Melkonyan, Arakelyan and Filipenko. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Ulyana A. Boyarskikh, boyarskih.u@gmail.com