%A Tao,Ping %A Hong,Liang %A Tang,Wenqing %A Lu,Qun %A Zhao,Yanrong %A Zhang,Si %A Ma,Lijie %A Xue,Ruyi %D 2021 %J Frontiers in Oncology %C %F %G English %K Keywor ds: Hepatocellular car cinoma,Tumor micr oenvir onment,landscape,heter ogeneity,pr ognosis %Q %R 10.3389/fonc.2020.574778 %W %L %M %P %7 %8 2021-January-22 %9 Original Research %+ Si Zhang,Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University,China,xue.ruyi@zs-hospital.sh.cn %+ Lijie Ma,Department of General Surgery, Shanghai Fifth People’s Hospital, Fudan University,China,xue.ruyi@zs-hospital.sh.cn %+ Lijie Ma,Department of General Surgery, Zhongshan Hospital (South), Shanghai Public Health Clinical Center, Fudan University,China,xue.ruyi@zs-hospital.sh.cn %+ Ruyi Xue,Department of Gastroenterology and Hepatology, Shanghai Institute of Liver Diseases, Zhongshan Hospital, Fudan University,China,xue.ruyi@zs-hospital.sh.cn %# %! Tumor immune landscape and heterogeneity in hepatocellular carcinoma %* %< %T Comprehensive Characterization of Immunological Profiles and Clinical Significance in Hepatocellular Carcinoma %U https://www.frontiersin.org/articles/10.3389/fonc.2020.574778 %V 10 %0 JOURNAL ARTICLE %@ 2234-943X %X BackgroundTherapies targeting immune molecules have rapidly been adopted and advanced the treatment of hepatocellular carcinoma (HCC). Nonetheless, no studies have reported a systematic analysis between immunological profiles and clinical significance in HCC.MethodsWe comprehensively investigated immune patterns and systematically correlated 22 types of both adaptive and innate immune cells with genomic characteristics and clinical outcomes based on 370 HCC patients from The Cancer Genome Atlas (TCGA) database through a metagene approach (known as CIBERSORT). Based on the Quantitative Pathology Imaging and Analysis System coupled with integrated high-dimensional bioinformatics analysis, we further independently validated six immune subsets (CD4+ T cells, CD8+ T cells, CD20+ B cells, CD14+ monocytes, CD56+ NK cells, and CD68+ macrophages), and shortlisted three (CD4+ T cells, CD8+ T cells, and CD56+ NK cells) of which to investigate their association with clinical outcomes in two independent Zhongshan cohorts of HCC patients (n = 258 and n = 178). Patient prognosis was further evaluated by Kaplan-Meier analysis and univariate and multivariate regression analysis.ResultsBy using the CIBERSORT method, the immunome landscape of HCC was constructed based on integrated transcriptomics analysis and multiplexed sequential immunohistochemistry. Further, the patients were categorized into four immune subgroups featured with distinct clinical outcomes. Strikingly, significant inter-tumoral and intra-tumoral immune heterogeneity was further identified according to the in-depth interrogation of the immune landscape.ConclusionThis work represents a potential useful resource for the immunoscore establishment for prognostic prediction in HCC patients.