Harnessing the Anti-Tumor Mediators in Mast Cells as a New Strategy for Adoptive Cell Transfer for Cancer

Abstract

The emergence of cancer immunotherapies utilizing adoptive cell transfer (ACT) continues to be one of the most promising strategies for cancer treatment. Mast cells (MCs) which occur throughout vascularized tissues, are most commonly associated with Type I hypersensitivity, bind immunoglobin E (IgE) with high affinity, produce anti-cancer mediators such as tumor necrosis factor alpha (TNF-α) and granulocyte macrophage colony-stimulating factor (GM-CSF), and generally populate the tumor microenvironments. Yet, the role of MCs in cancer pathologies remains controversial with evidence for both anti-tumor and pro-tumor effects. Here, we review the studies examining the role of MCs in multiple forms of cancer, provide an alternative, MC-based hypothesis underlying the mechanism of therapeutic tumor IgE efficacy in clinical trials, and propose a novel strategy for using tumor-targeted, IgE-sensitized MCs as a platform for developing new cellular cancer immunotherapies. This autologous MC cancer immunotherapy could have several advantages over current cell-based cancer immunotherapies and provide new mechanistic strategies for cancer therapeutics alone or in combination with current approaches.

Adoptive Cell Transfer for Cancer Immunotherapy

The use of autologous cells that can be targeted to tumors and induce apoptosis is an emerging therapeutic option to treat malignancies (1). From 2017 to 2018, there was a > 112% increase in the number of cell-based active agents in the global cancer immunotherapy pipeline. Most cells being investigated for autologous cancer immunotherapy have both pro- and anti-tumor mediators, their elevated numbers correlated with positive or negative patient outcomes, and strategies investigated to either inhibit their presence in tumors or utilize them for their anti-tumor properties. This strategy of adoptive cellular transfer (ACT) is typified by the use of autologous, peripheral T cells engineered ex vivo to express a transmembrane chimeric antigen receptor (CAR) composed of an extracellular, antigen-specific single-chain antibody and an intracellular T cell signaling domain (CAR T) (2). The use of CAR T-cell therapies has been approved by the Food and Drug Administration for children with acute lymphoblastic leukemia and adults with advanced lymphomas (3). Other T-cell based strategies, such as tumor-infiltrating lymphocyte (TIL) and engineered T cell receptor therapies are also being investigated (4). Several non-T immune cells also have potential anti-tumor activity. For example, dendritic cells (DC) modified in vitro with specific tumor-associated antigens to generate an immune response for cancer-cell elimination has led to clinical trials testing their safety and efficacy (5). Natural killer cells (NK) can eliminate cancer cells with surface markers associated with oncogenic transformation and have been investigated in clinical trials in patients with hematological malignancies or solid tumors (6). Peripheral blood eosinophils and neutrophils, containing potent mediators utilized by the immune system for pathogen destruction, have recently been demonstrated to have antitumorigenic activity (7, 8). As mentioned above, strategies to control tumor macrophages have resulted in numerous clinical trials in cancer patients to eliminate them alone or in combination with other therapies (9–11). Strategies to deplete macrophages are typified through inhibition of the CSF-1/CSF-1R signaling pathway. In general, depleting strategies have had limited success as unwanted removal of beneficial macrophages in non-tumor sights is a challenge (12). Conversely, other studies have hypothesized the anti-tumor capabilities of macrophages could be exploited and thus examined employing them as a form of ACT (13). While cytotoxic macrophages proved effective in animal models, this observation did not translate to humans (14). Recent strategies using CAR are intended to polarize pro-tumor and immunosuppressive M2 phenotype to a M1 phenotype with phagocytic functions, target cancer specific biomarkers, and induce an adaptive immune response (15, 16). In short, most cells being investigated as new platforms for cancer immunotherapy exert both pro- and anti-tumor effects. Therefore, the challenges moving forward in utilizing these cells is to remove the pro-tumor activity and/or enhance their anti-tumor functions. A summary table on the history of cell types being explored or used as cancer immunotherapy is shown in Table 1.

Challenges With Cell-Based Cancer Immunotherapies

While the numbers of autologous cells to target and inhibit cancer cell growth in vivo continues, so do the unanticipated roadblocks and challenges emerge. One challenge associated with CAR T cell therapies is the potentially life-threatening side-effect loosely defined as cytokine release syndrome (CRS). The CRS is induced by a systemic release of inflammatory cytokines associated with the T cell infusion and proliferation (and other T cell stimulants) (29). Also, the overwhelming majority of unique tumor antigens reside inside tumors, out of the reach of cells targeting them. This has led to efforts to identify and optimize delivery methods such as “in situ vaccination” at the tumor site hypothesized to release the inner tumor-associated antigens (30–33). Relatedly, most tumor antigens are promiscuous being found in and on cancerous and non-cancerous cells. This off-target phenomenon can result in serious or even fatal outcomes. An example of this is relates to an early trial in which T-cells were targeted to melanoma-associated antigen 3 (MAGE-A3) on metastatic cancers. Nervous system cells also express a similar MAGE-A12. As a result, T cells also invaded patients brain tissue resulting in the death of 2 out of 9 patients (34). The CAR T cell target CD19 is found on normal and malignant B cells. This can lead to lower immune cell numbers and side effects, such as a higher risk of infection when healthy cells are destroyed (35). Cancer cells are readily accessible to immune cells in blood as they circulate as individual cells or small clumps of cells compared to much larger solid tumors. Thus, another consideration in ACT development is the ability of the targeted cells to enter inside the tumor and release their anti-tumor mediators and killing at multiple locations.

Allergy, Cancer Risk, and the Emergence of Tumor Targeting IgE’s for Cancer Immunotherapy

Epidemiological studies investigating a correlation between atopic disease (e.g. serum IgE levels) and several types of cancer have demonstrated either a protective role or as a risk factor depending on the location (36–38). The retrospective, epidemiological observations that dominate the literature in general evaluated self-reported allergy histories, total IgE measurements, and/or skin prick tests and risks of cancer. An emerging area of research that suggests that patients with “ultralow” IgE serum levels have an associated with high rates of new malignancies not observed in mice (39–41). Specifically, patients with IgE deficiency and negative skin prick tests had a higher rate of malignancy than patients who had IgE deficiency with positive skin tests (41). This is important as a hallmark of IgE mediated functional responses of tissue mast cells (MCs) is the skin prick test which would support the possibility that IgE bound to MCs may have a role in tumor surveillance. As the epidemiological evidence linking atopic status to cancer risk continues to evolve (increased, decreased, or no association) so have the proposed hypotheses attempting to relate the possible mechanism linking allergy to cancer (37).

The development of atopy is initiated by the generation of IgE which binds FcεRI on MCs and basophils to induce allergic mediator release which induces allergy inflammation when encountering allergen. Tumor targeting IgE’s are being developed in an attempt to harness the diverse acquired responses mediated through IgE (e.g. parasite expulsion), the success of targeting cancer tumor markers with humanized IgG as a therapeutic strategy (42), and the epidemiological evidence suggesting a protective role for atopy against some malignancies (43). The IgE isotype has several potential advantages over IgG antibodies approved by the FDA on the market to treat various cancers such as the low serum levels of IgE (generally 100,000 fold lower than IgG) that result in less competition for FcεR occupancy, lack of inhibitory FcεR, and induces a different anti-tumor immune response compared to IgG (44, 45). Currently, there are over 10 IgE antibodies derived from patients or produced to target tumor-specific that have been assessed using in vitro and in vivo cancer models (Tables 2 and 3) For example, Fu et al. investigated the serum levels of IgE in patients with pancreatic cancer and revealed the cytotoxic effect of the purified IgE against this type of cancer cells (49). The synthesized human tumor-specific IgE’s such as MOv18 IgE for ovarian carcinoma (47), Trastuzumab and C6MH3-B1 IgE’s for breast (50), colon (58), and ovarian (47) cancers, Cetuximab IgE for breast and epidermoid carcinoma (52), anti-hCD20 for human B-cell lymphoma (53), anti-PSA for human prostate cancer (55), have been investigated by many research groups (Tables 2 and 3). Of note, the MOv18 IgE specific for the folate receptor alpha (FRα) was demonstrated to have anti-tumor effects in vitro and in vivo and is in phase 1 clinical trials testing with early data demonstrating demonstrated safety and efficacy in ovarian cancer patients (64). The survival of FRα-positive xenograft-bearing mice was increased in the presence of monocytes (48). Systemic treatment with MOv18 IgE induced TNF-α and IL-10 upregulation in tumors and significantly upregulated TNF-α, MCP-1 and IL-10 levels in bronchoalveolar lavage fluid using an in vivo xenograft model (65). Further in vitro studies examined the anti-tumor mechanism of IgE and demonstrated pro-inflammatory signals and tumor cell killing by human monocytes (66). An IgE targeting the tumor-associated antigen SLC3A2 induced FcεRI-mediated degranulation using a rodent cell line transfected with human receptor and triggered with SLC3A2-positive cell lines (58). The antibody did not trigger human basophil activation using unfractionated peripheral blood from cancer patients. In each of these studies, the mechanistic emphasis was on IgE-monocyte-mediated anti-tumor effects via IgE Fc-mediated ADCC.

MC in Cancer; Evidence for Both Anti- and Pro-Tumor Roles

As mentioned above, MCs are the final tissue effector cell in FcεRI-IgE allergic responses through the release of histamine and other noxious mediators. Their ability to release these mediators is also controlled by non-IgE and non-receptor mechanisms that are less common and include hypoxia, adenosine, and certain chemokines within the tumor milieu (67). MCs possess both pro-tumor and anti-tumor mediators, are found in large numbers in and around many types of tumors, and studies have variously suggested MCs should be targets for inhibition/depletion or exploited as an anti-tumorigenic strategy (67). There are various studies that showed MCs have an anti-tumorigenic role in ovarian cancer (68), clear-cell renal cell carcinoma (ccRCC) (69), B cell lymphoma (70), skin cancer (71, 72), renal cancer (73), oral squamous cell carcinoma (OSCC) (74, 75), non-small-cell lung cancer (NSCLC) (76, 77), intestine cancer (71, 78), lung cancer (79), melanoma (80–82), prostate cancer (83–85), colorectal cancer (86), and breast cancer (57, 87–92) (Figure 1A). Patients with elevated MC counts had a significantly better event-free survival (EFS) compared to those with fewer MCs in several tumor types. Several unique phenotypic characteristics of MCs could contribute mechanistically to anti-tumor effects. Human MCs are unique in that they have prestored, releasable (through FcεRI) tumor necrosis factor alpha (TNF-α), histamine, and tryptase within their granules. The biggest impediment to using TNF-α as an anti-cancer agent is its systemic toxicity and strategies that limit its systemic distribution through local administration in patients have been investigated (93). Histamine induces the differentiation of immature myeloid cells and suppresses their ability to support the growth of tumor allografts (71). Increased histidine decarboxylase (which produces histamine) gene expression is associated with better relapse-free and overall survival in breast cancer patients and histamine treatment reduces tumor growth and increased apoptosis in xenograft breast cancer models (94). Mast cell tryptase alters the morphology and reduces the proliferation of human melanoma cells (82). We and others have demonstrated human MC release copious amounts (2,500-4,000 pg/ml from 10⁵ cells) of granulocyte-macrophage colony-stimulating factor (GM-CSF); also, an anti-tumor mediator investigated in over 50 clinical trials (95). Mast cells showed direct antitumor effects in vitro and decreased angiogenesis and recruitment of NK and T cells in vivo (80).

Figure 1

In contrast, other studies have suggested a pro-tumorigenic role of MCs in different cancers (Figure 1A) with increased MC populations in certain tumor microenvironments associated with poor patient prognosis (96–106). These studies investigated the expression of MC markers (e.g. chymase/tryptase expression, FcεRI, c-KIT, etc.) in tumor tissues using immunohistochemistry, flow cytometry, immunoblotting, or RT-PCR techniques (67, 107, 108). In general, most published studies that attribute a pro-tumorigenic role for MC rely on correlations with increased MC numbers at a single time point, dependent on the tumor type, stage, and cancer microenvironment-and patient outcomes (Figure 1B). A “snapshot” analysis demonstrating an increase or decrease in MC numbers based on immunohistochemistry and subsequent association with a specific prognosis cannot be relied on to predict if these cells have a beneficial or deleterious effect. Observing an increase in MC numbers paralleled by a poor prognosis (or vice versa) demonstrates a correlation, not a causation between numbers and prognosis. Studies are needed to assess the effects selectively knocking down (i.e. CRISPR) the pro-tumorigenic and/or upregulation of anti-tumorigenic mediators from human MCs. Nonetheless, MCs are one of the first cells to infilitrate the tumor microenvironment and possess such a wide range of receptors and molecules with diverse functions that mediate tumor responses that adds to the controversial role they play in the disease (109).

Another issue surrounding the analysis of the MCs role in cancers relates to conclusions drawn from MC knockout studies, with constraints in results observed depending on the model (110–112). In some cases, a pro- and anti-tumor effect was observed in the same tumors (67, 113, 114). In addition, differences in MC phenotypic and functional responses between mice and humans have been well documented (111, 115–123). For example, Fcγ receptor expression and functional responses mediated by them on mouse and human MCs and monocytes are vastly different (124–126). Further, mouse MCs have a diverse range of various proteases (127) while human MCs principally express three proteases (tryptase, chymase, and carboxypeptidase-A) (128). Histamine is released from human MCs, while both serotonin and histamine are liberated in reasonable amounts from MCs in mice, and both contribute to the physiological effects in anaphylactic reactions, respectively in these species. Interleukin-3 has a profound effect on murine MC differentiation and function not observed with human MCs. Of course, cancer therapeutic strategies require animal models to determine efficacy of drug targets, safety, biodistribution, etc. But caution must be taken when extrapolating data from mouse models of cancer, especially when focused on MC numbers and MC Fc-specific mechanisms.

Could MCs Mediate the Efficacy of Anti-Tumor IgE’s and in IgE Tumor Surveillance?

The mechanisms underlying the anti-tumor effects of therapeutic IgE’s are mostly attributed to monocyte and macrophage infiltration and subsequent IgE-mediated activation of these cells around tumors (56, 65, 129, 130). This hypothesized mechanism seems counter-intuitive to current evidence that demonstrates tumor-infiltrating myeloid cells promote, rather than inhibit-cancer progression (10). FcϵRIα-positive macrophages have been identified as critical infiltrating cells that induce tumor progression in squamous cell carcinoma (131) [although evidence is presented that the anti-FcϵRI antibody used in this study was not specific for FcϵRI on macrophages (132)]. As is the case with MC, macrophages may initiate, promote, or suppress the development of cancer, possess both pro (e.g. VEGF, EGF, and TGF-β) and inhibitory (e.g. nitric oxide), and have been implicated to mediate angiogenesis, invasiveness, metastasis, and acquired resistance to therapeutic strategies largely based on correlations between cell numbers and patient outcomes (133–135).

The hypothesis that monocytes/macrophages mediate anti-tumor efficacy to tumor IgE’s is also premised on the surface expression of FcεRI on monocytes/macrophages that controls their effector functions. However, the expression of FcεRI on primary human monocytes has been reported to be low (<10% in non-atopic patients), or not at all, compared to primary human MC and the expression level on monocytes is 10 to 100-fold less than observed for peripheral blood basophils from the same subjects (136, 137). While human monocytes can be manipulated to increase FcεRI expression in vitro (66) it is unknown if primary, tissue macrophages express FcεRI to any degree in humans. It also cannot be assumed the expression of IgE receptors will stay the same after entry and maturation in the tissues as monocytes undergo phenotypic changes upon tissue entry as they mature into macrophages (138). Others have shown human tissue macrophages do not express FcεRI (139–141). Here, another difference between species relates to reports in rodent studies that support the conclusion that macrophages can mediate anaphylaxis in mice; a phenomenon not described in humans (142, 143). One study showed that the responses of human alveolar macrophages involving IgE in vitro (144, 145) was most probably mediated by FcεRII (CD23) which has lower affinity for IgE, is distinct functionally from FcεRI (146, 147), and would help explain the RBC-rosetting most of these older studies used to determine IgE binding (148, 149). Lastly, other tissue cells besides MC have been reported to express FcεRI (e.g. Langerhan cells) and the low affinity receptor for IgE (150). Human basophils (and in some cases eosinophils) express FcεRI they are not normally found in tissue but are recruited following certain pathological mechanisms (151). Human eosinophils have demonstrated FcεRI expression (and have anti-tumor properties (7)) but only from donors with eosinophilia and lymphomas (152). Thus, the likelihood of tumor specific IgE binding to human monocyte-derived, tissue macrophages with unknown FcεRI expression to mediate effects seems less likely given many other IgE binding cells are present. MCs [with almost 100% FcεRI expression (57)] are as abundant or more abundant in the tumor microenvironment than macrophages depending on the tumor type. For example, the rodent form of IgE MOv18 reduced lung metastases in a syngeneic rat tumor model expressing human FRα which was attributed to TNFα, IL-10, and MCP-1 released by MOv18-triggered monocytes (56). However, the cytokine profile induced in BAL by MOv18 (TNFα, MCP-1, and IL-10) could very likely include a contribution from lung MCs which we and many others have shown produce such cytokines upon FcεRI stimulation (57, 153–156). We thus propose the binding of tumor targeted IgE Fc to human MC FcεRI and subsequent triggering of this receptor upon tumor engagement mediate the anti-tumor effects of therapeutic IgE’s given the demonstrated high amounts of FcεRI on primary human MCs in the tumor milieu (157), the high numbers of FcεRI (>1 x 10⁵/cell) that require only ≈100 receptors for full activation (67, 158), the affinity of this interaction (159), the juxtaposition of MCs with cancers cells (67), and the anti-tumor mediators released from MCs (160). Infusion of IgE into patients is hypothesized to increase surface expression of MC FcεRI as this receptor is dependent on serum IgE levels (158, 161).

Studies Using Tumor Targeting IgE’s and MCs

Attempts to utilize anti-tumor mediators from MCs for cancer cell targeting was first examined using a mouse–human chimeric IgE specific for CD20 and the epithelial antigen MUC1. Cord blood-derived MCs sensitized with anti-hCD20 IgE are cytotoxic to CD20 tumor cells in vitro (53). We used adipose-derived mast cells (ADMC) sensitized with human anti-HER2/neu IgE which bound to and released MC mediators when incubated with HER2/neu-positive human breast cancer cells (SK-BR-3 and BT-474) resulting in TNF-α mediated, tumor cell apoptosis (57). Importantly, monomeric (shed) HER2/neu and serums from HER2+ breast cancer patients did not induce ADMC degranulation, suggesting that such an interaction will not trigger systemic anaphylaxis.

Will MC be Added to Growing List of Tumor Targeting Cellular Immunotherapy?

As discussed above the variety of cell types being investigated as new strategies for cancer immunotherapy continues to increase. MCs are similar to tumor associated macrophages as discussed above in that both have both pro- and anti-tumor capabilities and correlative studies led to assumptions regarding their role in various cancers (16). Because of this, initial efforts were aimed at depleting or repolarizing these cells as a therapeutic, anti-tumor strategy. MCs are presently at the apparently contradictory position in which rationale arguments could be made for inhibiting their numbers in the tumor milieu or increasing their numbers and harnessing their natural associated anti-tumor mediators within them. Yet from our perspective informed decisions as to deplete, increase, or repolarize MCs cannot be made until more studies assess their functional role in cancer models. As with human macrophages, human MC may need to be “repolarized” from a Type I hypersensitivity-associated cell type to an anti-cancer cell through up or down regulation of certain mediators. To this end, transfection/transduction of primary MCs has only recently been achieved using human peripheral blood derived MCs (162). The conditions that will now allow us to manipulate MC so that maximal anti-tumor activity is conferred and/or potential deleterious mediators can be deleted are being explored in our laboratory.

We propose human MCs as another cell type to be used in ACT for cancers in which tumor specific IgE’s are available or could be made. To do this, autologous MCs could be obtained from adipose tissue or cultured from peripheral blood and expanded ex vivo. Anti-tumor capabilities could be increased or deleterious mediators downregulated during expansion. FcεRI-positive MCs are then sensitized with IgE targeting antigens found on tumors. The tumor targeting MCs would then injected into the patient and become active upon FcεRI-IgE crosslinking. This autologous MC cancer immunotherapy (Figure 2) would result in the release of anti-tumor mediators within the tumor milieu (see graphical abstract). Recently we have demonstrated up to 6 x 10⁶ human ADMC can be injected i.v. into mice with no toxicological effects. The ADMC, sensitized with human IgE recognizing the breast cancer antigen HER2/neu, shrink HER2/neu-positive tumors in vivo using a xenograft mouse model (manuscript submitted). Since human GM-CSF is not active in mice (163) the anti-tumor effects we have observed are expected to be stronger in humans in which GM-CSF would be fully active (164). This approach may enhance anti-tumor immunity through epitope spreading of cancer antigens. Importantly, this strategy may spurn new areas of research through transformation or manufacturing of tumor-targeted IgE’s. Harvesting adipose tissue from patients is not difficult, commonly performed, and increasingly being used for a wide variety of clinical applications (165, 166). Recently, we have demonstrated peripheral blood, CD34-positive stem cell derived MC also have anti-tumor activity providing a second source of autologous MCs (data not shown).

Figure 2

Advantages of AMCIT as a New Cancer Immunotherapy Approach

There is a growing list of human IgE antibodies targeting cancer antigens that have been fully characterized which provide the targeting needed to transport the MCs to the tumor sites (43). It should be noted with caution when examining anti-tumor effects experimentally that the use of certain tumor targeted IgE’s is limited as human IgE does not bind mouse FcεRI receptors (167). Second, the in vitro incubation of MCs with IgE for targeting is extremely stable (168) and allows for the saturation of FcεRI binding, thus maximizing the effect while preventing patient IgE binding. IgE also stays bound to MCs for several months in vivo (169–173). Third, the adipose stem cells may be cryopreserved, reconstituted, and differentiated into ADMC while retaining expression of introduced genetic modules (data not shown). This is an important characteristic, as it greatly enhances the “logistics” of the potential therapy in that patient cells could be transduced, cryopreserved for shipping, and reconstituted when needed for therapy. Fourth, MC activation is hypothesized to induce acute inflammation and destruction of cancer cells in the tumor microenvironment due to the release of multiple mediators. The presence of dead tumor cells would allow uptake and presentation of tumor antigens by antigen presenting cells as with dendritic cells that elicit an adaptive, long lasting immune response not only to the targeted antigen but also to other tumor antigens due to epitope spreading. This would increase due to the local release of GM-CSF from MCs (174, 175) and the release of regulatory T-cell function suppressors (176). Of course, the use of tumor IgE’s alone or using tumor IgE-sensitized MCs as proposed here has the obvious potential to induce a systemic allergic response. Strategies to delete select mediators in human MCs are underway but with caution as it is simply not known if those with potential “toxic” side effects also have potent anti-tumor effects. Lastly, this strategy has the potential to circumvent challenges associated with current ACT strategies in which hyperactive T cells create a cytokine storm (177), reduced chances of off-target binding (not expressed on normal cells; e.g. CD19) (178), and avoid the lack of expansion and/or persistence of autologous cells (as with NK cells) (179).

Conclusions and Future Directions

While the role of MCs in all cancer pathogenesis is still unclear, future studies are needed to examine if ex vivo-derived MCs possess anti-tumor capabilities. Questions remain regarding the possibility of systemic MC activation, although this issue can be overcome as discussed above with prophylactic anti-histamines, as is common practice (180–183). An alternative clinical strategy could be to first add IgE, followed by the MCs. Antigen levels on target cells may vary in patients, which could minimize cell targeting and activation. It is not anticipated that high levels of antigen will be needed, as human MCs require ≈100 FcεRI receptors to aggregate for a full activation response (158) and all FcεRI will be saturated so that patient IgE binding will not occur. Shed antigen in serum may also “mask” the MC-bound IgE without inducing degranulation, however blocking future binding. That said, this remains unlikely given the in vivo studies using IgE antibodies to tumor antigens do not suggest masking (50, 58, 184, 185).

There are myriad reasons to speculate on the many potential roadblocks that could arise during the development of the AMCIT as a new cancer immunotherapy strategy. But it is important to highlight similar misgivings, inaccurate predictions regarding toxicity, and major setbacks in the early years of CAR T-cell therapy (177, 186, 187). The emergence of CAR-T immunotherapy was met with skepticism and progressed only gradually based on incremental insights over many years. Even though unexpected toxic effects in Phase 1 studies can quell any new therapy, the unfortunate reality is that it can take time to distinguish toxic effects as was the case in the first CART-19 trials (186, 188, 189). The point is that it is impossible to predict what, if any, side effects might occur in vivo with ADMC until studies to assess their role are performed. We believe the need for novel therapies that bring new mechanisms to combat cancer pathologies are important to investigate given the continued morbidity and mortality associated with this disease.

Funding

CK was funded by NIH/NCI grant number 1R15CA246430, Specialized Center of Research grant and Pilot grant from UNC-Chapel Hill, Lineberger Cancer Center, and Giant Steps grant from UNCG. DM is supported by the Division of Intramural Research, NIH/NIAID. TK is funded by NIH R01HL155986.

Publisher’s Note

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References

• 1

  EmensLAAsciertoPADarcyPKDemariaSEggermontAMMRedmondWLet al. Cancer Immunotherapy: Opportunities and Challenges in the Rapidly Evolving Clinical Landscape. Eur J Cancer (2017) 81:116–29. doi: 10.1016/j.ejca.2017.01.035

  • CrossRef
  • Google Scholar

• 2

  JuneCHO’ConnorRSKawalekarOUGhassemiSMiloneMC. CAR T Cell Immunotherapy for Human Cancer. Science (2018) 359(6382):1361–5. doi: 10.1126/science.aar6711

  • CrossRef
  • Google Scholar

• 3

  TranELongoDLUrbaWJ. A Milestone for CAR T Cells. N Engl J Med (2017) 377(26):2593–6. doi: 10.1056/NEJMe1714680

  • CrossRef
  • Google Scholar

• 4

  GreenbergPD. How Cellular Immunotherapies Are Changing the Outlook for Cancer Patients. InstituteCR. In: editor. Cancer Research Institute: CRI Documents (2022).

  • Google Scholar

• 5

  HuberADammeijerFAertsJVromanH. Current State of Dendritic Cell-Based Immunotherapy: Opportunities for In Vitro Antigen Loading of Different DC Subsets? Front Immunol (2018) 9:2804. doi: 10.3389/fimmu.2018.02804

  • CrossRef
  • Google Scholar

• 6

  ShimasakiNJainACampanaD. NK Cells for Cancer Immunotherapy. Nat Rev Drug Discov (2020) 19(3):200–18. doi: 10.1038/s41573-019-0052-1

  • CrossRef
  • Google Scholar

• 7

  ReichmanHItanMRozenbergPYarmolovskiTBrazowskiEVarolCet al. Activated Eosinophils Exert Antitumorigenic Activities in Colorectal Cancer. Cancer Immunol Res (2019) 7(3):388–400. doi: 10.1158/2326-6066.CIR-18-0494

  • CrossRef
  • Google Scholar

• 8

  FurumayaCMartinez-SanzPBoutiPKuijpersTWMatlungHL. Plasticity in Pro- and Anti-Tumor Activity of Neutrophils: Shifting the Balance. Front Immunol (2020) 11:2100. doi: 10.3389/fimmu.2020.02100

  • CrossRef
  • Google Scholar

• 9

  WuKLinKLiXYuanXXuPNiPet al. Redefining Tumor-Associated Macrophage Subpopulations and Functions in the Tumor Microenvironment. Front Immunol (2020) 11:1731. doi: 10.3389/fimmu.2020.01731

  • CrossRef
  • Google Scholar

• 10

  AllavenaPAnfrayCUmmarinoAAndonFT. Therapeutic Manipulation of Tumor-Associated Macrophages: Facts and Hopes From a Clinical and Translational Perspective. Clin Cancer Res (2021) 27(12):3291–7. doi: 10.1158/1078-0432.CCR-20-1679

  • CrossRef
  • Google Scholar

• 11

  ChengNBaiXShuYAhmadOShenP. Targeting Tumor-Associated Macrophages as an Antitumor Strategy. Biochem Pharmacol (2021) 183:114354. doi: 10.1016/j.bcp.2020.114354

  • CrossRef
  • Google Scholar

• 12

  PohARErnstM. Targeting Macrophages in Cancer: From Bench to Bedside. Front Oncol (2018) 8:49. doi: 10.3389/fonc.2018.00049

  • CrossRef
  • Google Scholar

• 13

  AndersonNRMinutoloNGGillSKlichinskyM. Macrophage-Based Approaches for Cancer Immunotherapy. Cancer Res (2021) 81(5):1201–8. doi: 10.1158/0008-5472.CAN-20-2990

  • CrossRef
  • Google Scholar

• 14

  AndreesenRHennemannBKrauseSW. Adoptive Immunotherapy of Cancer Using Monocyte-Derived Macrophages: Rationale, Current Status, and Perspectives. J Leuk Biol (1998) 64(4):419–26. doi: 10.1002/jlb.64.4.419

  • CrossRef
  • Google Scholar

• 15

  KlichinskyMRuellaMShestovaOLuXMBestAZeemanMet al. Human Chimeric Antigen Receptor Macrophages for Cancer Immunotherapy. Nat Biotechnol (2020) 38(8):947–53. doi: 10.1038/s41587-020-0462-y

  • CrossRef
  • Google Scholar

• 16

  MukhopadhyayM. Macrophages Enter CAR Immunotherapy. Nat Methods (2020) 17(6):561. doi: 10.1038/s41592-020-0862-4

  • CrossRef
  • Google Scholar

• 17

  ColeyWB. The Classic: The Treatment of Malignant Tumors by Repeated Inoculations of ErysipelasWith a Report of Ten Original Cases. Clin Orthop Relat Res (1991) 262:3–11. doi: 10.1097/00003086-199101000-00002

  • CrossRef
  • Google Scholar

• 18

  ZinkernagelRMDohertyPC. Immunological Surveillance Against Altered Self Components by Sensitised T Lymphocytes in Lymphocytes Choriomeningitis. Nature (1974) 251(5475):547–8. doi: 10.1038/251547a0

  • CrossRef
  • Google Scholar

• 19

  HerbermanRBNunnMEHoldenHTLavrinDH. Natural Cytotoxic Reactivity of Mouse Lymphoid Cells Against Syngeneic and Allogeneic Tumors. II. Characterization of Effector Cells. Int J Cancer (1975) 16(2):230–9. doi: 10.1002/ijc.2910160205

  • CrossRef
  • Google Scholar

• 20

  Organon Teknika Corporation. Summary for Basis of Approval (1998). Available at: http://waybackarchive-itorg/7993/20170723144330/https://wwwfdagov/downloads/BiologicsBloodVaccines/Vaccines/ApprovedProducts/UCM101490pdf (Accessed November 1, 2021).

  • Google Scholar

• 21

  van der BruggenPTraversariCChomezPLurquinCDe PlaenEVan den EyndeBet al. A Gene Encoding an Antigen Recognized by Cytolytic T Lymphocytes on a Human Melanoma. Science (1991) 254(5038):1643–7. doi: 10.1126/science.1840703

  • CrossRef
  • Google Scholar

• 22

  Cancer Research Institute. FDA Approval Timeline of Active Immunotherapies. Available at: https://wwwcancerresearchorg/scientists/immuno-oncology-landscape/fda-approval-timeline-of-active-immunotherapies (Accessed August 5, 2021).

  • Google Scholar

• 23

  GardnerTAElzeyBDHahnNM. Sipuleucel-T (Provenge) Autologous Vaccine Approved for Treatment of Men With Asymptomatic or Minimally Symptomatic Castrate-Resistant Metastatic Prostate Cancer. Hum Vaccin Immunother (2012) 8(4):534–9. doi: 10.4161/hv.19795

  • CrossRef
  • Google Scholar

• 24

  FecciPEMitchellDAArcherGEMorseMALyerlyHKBignerDDet al. The History, Evolution, and Clinical Use of Dendritic Cell-Based Immunization Strategies in the Therapy of Brain Tumors. J Neurooncol (2003) 64(1-2):161–76. doi: 10.1007/BF02700031

  • CrossRef
  • Google Scholar

• 25

  Clinincal trials phase II. Dendritic Cell Vaccine for Patients With Brain Tumors. Available at: https://clinicaltrialsgov/ct2/show/NCT01204684 (Accessed June 1, 2021).

  • Google Scholar

• 26

  MohantyRChowdhuryCRAregaSSenPGangulyPGangulyN. CAR T Cell Therapy: A New Era for Cancer Treatment (Review). Oncol Rep (2019) 42(6):2183–95. doi: 10.3892/or.2019.7335

  • CrossRef
  • Google Scholar

• 27

  DeNardoDGRuffellB. Macrophages as Regulators of Tumour Immunity and Immunotherapy. Nat Rev Immunol (2019) 19(6):369–82. doi: 10.1038/s41577-019-0127-6

  • CrossRef
  • Google Scholar

• 28

  U.S. Food and Drug Administration. IMLYGIC (Talimogene Laherparepvec, Amgen Inc.). Available at: https://wwwfdagov/vaccines-blood-biologics/cellular-gene-therapy-products/imlygic-talimogene-laherparepvec (Accessed November 21, 2021).

  • Google Scholar

• 29

  HirayamaAVTurtleCJ. Toxicities of CD19 CAR-T Cell Immunotherapy. Am J Hematol (2019) 94(S1):S42–s49. doi: 10.1002/ajh.25445

  • CrossRef
  • Google Scholar

• 30

  HammerichLBhardwajNKohrtHEBrodyJD. In Situ Vaccination for the Treatment of Cancer. Immunotherapy (2016) 8(3):315–30. doi: 10.2217/imt.15.120

  • CrossRef
  • Google Scholar

• 31

  HanSWangW-WRozaliETohHC. Defining Precision Cellular Immunotherapy—Seeking Biomarkers to Predict and Optimize Outcomes of T Cell Therapies in Cancer. Precis Cancer Med (2019) 2:25. doi: 10.21037/pcm.2019.07.02

  • CrossRef
  • Google Scholar

• 32

  RileyRSJuneCHLangerRMitchellMJ. Delivery Technologies for Cancer Immunotherapy. Nat Rev Drug Discov (2019) 18(3):175–96. doi: 10.1038/s41573-018-0006-z

  • CrossRef
  • Google Scholar

• 33

  ZhangHYeZLYuanZGLuoZQJinHJQianQJ. New Strategies for the Treatment of Solid Tumors With CAR-T Cells. Int J Biol Sci (2016) 12(6):718–29. doi: 10.7150/ijbs.14405

  • CrossRef
  • Google Scholar

• 34

  MorganRAChinnasamyNAbate-DagaDGrosARobbinsPFZhengZet al. Cancer Regression and Neurological Toxicity Following Anti-MAGE-A3 TCR Gene Therapy. J Immunother (2013) 36(2):133–51. doi: 10.1097/CJI.0b013e3182829903

  • CrossRef
  • Google Scholar

• 35

  American Cancer Society. CAR T-Cell Therapy and Its Side Effects. Available at: https://wwwcancerorg/treatment/treatments-and-side-effects/treatment-types/immunotherapy/car-t-cell1html (Accessed June 10, 2021).

  • Google Scholar

• 36

  CuiYHillAW. Atopy and Specific Cancer Sites: A Review of Epidemiological Studies. Clin Rev Allergy Immunol (2016) 51(3):338–52. doi: 10.1007/s12016-016-8559-2

  • CrossRef
  • Google Scholar

• 37

  KozłowskaRBożekAJarząbJ. Association Between Cancer and Allergies. Allergy Asthma Clin Immunol (2016) 12:39–9. doi: 10.1186/s13223-016-0147-8

  • CrossRef
  • Google Scholar

• 38

  BozekAJarzabJMielnikMBogaczAKozlowskaRMangoldD. Can Atopy Have a Protective Effect Against Cancer? PLoS One (2020) 15(2):e0226950. doi: 10.1371/journal.pone.0226950

  • CrossRef
  • Google Scholar

• 39

  FerastraoaruDJordakievaGJensen-JarolimE. The Other Side of the Coin: IgE Deficiency, A Susceptibility Factor for Malignancy Occurrence. World Allergy Organ J (2021) 14(1):100505. doi: 10.1016/j.waojou.2020.100505

  • CrossRef
  • Google Scholar

• 40

  FerastraoaruDBaxHJBergmannCCapronMCastellsMDombrowiczDet al. AllergoOncology: Ultra-Low IgE, a Potential Novel Biomarker in Cancer-a Position Paper of the European Academy of Allergy and Clinical Immunology (EAACI). Clin Transl Allergy (2020) 10(32):020–00335. doi: 10.1186/s13601-020-00335-w

  • CrossRef
  • Google Scholar

• 41

  FerastraoaruDRosenstreichD. IgE Deficiency Is Associated With High Rates of New Malignancies: Results of a Longitudinal Cohort Study. J Allergy Clin Immunol Pract (2020) 8(1):413–5. doi: 10.1016/j.jaip.2019.06.031

  • CrossRef
  • Google Scholar

• 42

  GoydelRSRaderC. Antibody-Based Cancer Therapy. Oncogene (2021) 4(10):021–01811.

  • Google Scholar

• 43

  LeohLSDaniels-WellsTRPenichetML. IgE Immunotherapy Against Cancer. Curr Top Microbiol Immunol (2015) 388:109–49. doi: 10.1007/978-3-319-13725-4_6

  • CrossRef
  • Google Scholar

• 44

  ClynesRATowersTLPrestaLGRavetchJV. Inhibitory Fc Receptors Modulate In Vivo Cytoxicity Against Tumor Targets. Nat Med (2000) 6(4):443–6. doi: 10.1038/74704

  • CrossRef
  • Google Scholar

• 45

  NimmerjahnFRavetchJV. Antibodies, Fc Receptors and Cancer. Curr Opin Immunol (2007) 19(2):239–45. doi: 10.1016/j.coi.2007.01.005

  • CrossRef
  • Google Scholar

• 46

  SunLKLiouRSSunNCGossettLASunCDavisFMet al. Transfectomas Expressing Both Secreted and Membrane-Bound Forms of Chimeric IgE With Anti-Viral Specificity. J Immunol (1991) 146(1):199–205.

  • Google Scholar

• 47

  GouldHJMackayGAKaragiannisSNO’TooleCMMarshPJDanielBEet al. Comparison of IgE and IgG Antibody-Dependent Cytotoxicity In Vitro and in a SCID Mouse Xenograft Model of Ovarian Carcinoma. Eur J Immunol (1999) 29(11):3527–37. doi: 10.1002/(SICI)1521-4141(199911)29:11<3527::AID-IMMU3527>3.0.CO;2-5

  • CrossRef
  • Google Scholar

• 48

  KaragiannisSNWangQEastNBurkeFRiffardSBracherMGet al. Activity of Human Monocytes in IgE Antibody-Dependent Surveillance and Killing of Ovarian Tumor Cells. Eur J Immunol (2003) 33(4):1030–40. doi: 10.1002/eji.200323185

  • CrossRef
  • Google Scholar

• 49

  FuSLPierreJSmith-NorowitzTAHaglerMBowneWPincusMRet al. Immunoglobulin E Antibodies From Pancreatic Cancer Patients Mediate Antibody-Dependent Cell-Mediated Cytotoxicity Against Pancreatic Cancer Cells. Clin Exp Immunol (2008) 153(3):401–9. doi: 10.1111/j.1365-2249.2008.03726.x

  • CrossRef
  • Google Scholar

• 50

  KaragiannisPSingerJHuntJGanSKRudmanSMMechtcheriakovaDet al. Characterisation of an Engineered Trastuzumab IgE Antibody and Effector Cell Mechanisms Targeting HER2/neu-Positive Tumour Cells. Cancer Immunol Immunother (2009) 58(6):915–30. doi: 10.1007/s00262-008-0607-1

  • CrossRef
  • Google Scholar

• 51

  RudmanSMJosephsDHCambrookHKaragiannisPGilbertAEDodevTet al. Harnessing Engineered Antibodies of the IgE Class to Combat Malignancy: Initial Assessment of FcvarepsilonRI-Mediated Basophil Activation by a Tumour-Specific IgE Antibody to Evaluate the Risk of Type I Hypersensitivity. Clin Exp Allergy (2011) 41(10):1400–13. doi: 10.1111/j.1365-2222.2011.03770.x

  • CrossRef
  • Google Scholar

• 52

  SpillnerEPlumMBlankSMieheMSingerJBrarenI. Recombinant IgE Antibody Engineering to Target EGFR. Cancer Immunol Immunother (2012) 61(9):1565–73. doi: 10.1007/s00262-012-1287-4

  • CrossRef
  • Google Scholar

• 53

  TeoPZUtzPJMollickJA. Using the Allergic Immune System to Target Cancer: Activity of IgE Antibodies Specific for Human CD20 and MUC1. Cancer Immunol Immunother (2012) 61(12):2295–309. doi: 10.1007/s00262-012-1299-0

  • CrossRef
  • Google Scholar

• 54

  DanielsTRLeuchterRKQuinteroRHelgueraGRodriguezJAMartinez-MazaOet al. Targeting HER2/neu With a Fully Human IgE to Harness the Allergic Reaction Against Cancer Cells. Cancer Immunol Immunother (2012) 61(7):991–1003. doi: 10.1007/s00262-011-1150-z

  • CrossRef
  • Google Scholar

• 55

  Daniels-WellsTRHelgueraGLeuchterRKQuinteroRKozmanMRodriguezJAet al. A Novel IgE Antibody Targeting the Prostate-Specific Antigen as a Potential Prostate Cancer Therapy. BMC Cancer (2013) 13:195. doi: 10.1186/1471-2407-13-195

  • CrossRef
  • Google Scholar

• 56

  JosephsDHBaxHJDodevTGeorgouliMNakamuraMPellizzariGet al. Anti-Folate Receptor-Alpha IgE But Not IgG Recruits Macrophages to Attack Tumors via TNFalpha/MCP-1 Signaling. Cancer Res (2017) 77(5):1127–41. doi: 10.1158/0008-5472.CAN-16-1829

  • CrossRef
  • Google Scholar

• 57

  PlotkinJDEliasMGFereydouniMDaniels-WellsTRDellingerALPenichetMLet al. Human Mast Cells From Adipose Tissue Target and Induce Apoptosis of Breast Cancer Cells. Front Immunol (2019) 10:138. doi: 10.3389/fimmu.2019.00138

  • CrossRef
  • Google Scholar

• 58

  PellizzariGMartinezOCrescioliSPageRDi MeoAMeleSet al. Immunotherapy Using IgE or CAR T Cells for Cancers Expressing the Tumor Antigen SLC3A2. J Immunother Cancer (2021) 9(6):2020–002140. doi: 10.1136/jitc-2020-002140

  • CrossRef
  • Google Scholar

• 59

  SaulLJosephsDHCutlerKBradwellAKaragiannisPSelkirkCet al. Comparative Reactivity of Human IgE to Cynomolgus Monkey and Human Effector Cells and Effects on IgE Effector Cell Potency. MAbs (2014) 6(2):509–22. doi: 10.4161/mabs.27828

  • CrossRef
  • Google Scholar

• 60

  ClinicalTrials. Phase I Study of MOv18 IgE, a First in Class Chimeric IgE Antibody in Patients With Advanced Solid Tumours. Available at: https://clinicaltrialsgov/ct2/show/NCT02546921 (Accessed may 7, 2021).

  • Google Scholar

• 61

  FazekasJFurdosISingerJJensen-JarolimE. Why Man’s Best Friend, the Dog, Could Also Benefit From an Anti-HER-2 Vaccine. Oncol Lett (2016) 12(4):2271–6. doi: 10.3892/ol.2016.5001

  • CrossRef
  • Google Scholar

• 62

  GrujicMPaivandyAGustafsonAMThomsenAROhrvikHPejlerG. The Combined Action of Mast Cell Chymase, Tryptase and Carboxypeptidase A3 Protects Against Melanoma Colonization of the Lung. Oncotarget (2017) 8(15):25066–79. doi: 10.18632/oncotarget.15339

  • CrossRef
  • Google Scholar

• 63

  WilliamsIPCrescioliSSowHSBaxHJHobbsCIlievaKMet al. In Vivo Safety Profile of a CSPG4-Directed IgE Antibody in an Immunocompetent Rat Model. MAbs (2020) 12(1):1685349. doi: 10.1080/19420862.2019.1685349

  • CrossRef
  • Google Scholar

• 64

  SpicerJBasuBMontesABanerjiUKristeleitRVealGJet al. Abstract CT141: Phase 1 Trial of MOv18, A First-in-Class IgE Antibody Therapy for Cancer. Cancer Res (2020) 80(16 Supplement):CT141. doi: 10.1158/1538-7445.AM2020-CT141

  • CrossRef
  • Google Scholar

• 65

  NakamuraMSouriEAOsbornGLaddachRChauhanJStavrakaCet al. IgE Activates Monocytes From Cancer Patients to Acquire a Pro-Inflammatory Phenotype. Cancers (Basel) (2020) 12(11):3376. doi: 10.3390/cancers12113376

  • CrossRef
  • Google Scholar

• 66

  PellizzariGHoskinCCrescioliSMeleSGotovinaJChiaruttiniGet al. IgE Re-Programs Alternatively-Activated Human Macrophages Towards Pro-Inflammatory Anti-Tumoural States. EBioMedicine (2019) 43:67–81. doi: 10.1016/j.ebiom.2019.03.080

  • CrossRef
  • Google Scholar

• 67

  VarricchiGGaldieroMRLoffredoSMaroneGIannoneRMaroneGet al. Are Mast Cells MASTers in Cancer? Front Immunol (2017) 8:424. doi: 10.3389/fimmu.2017.00424

  • CrossRef
  • Google Scholar

• 68

  ChanJKMagistrisALoizziVLinFRutgersJOsannKet al. Mast Cell Density, Angiogenesis, Blood Clotting, and Prognosis in Women With Advanced Ovarian Cancer. Gynecol Oncol (2005) 99(1):20–5. doi: 10.1016/j.ygyno.2005.05.042

  • CrossRef
  • Google Scholar

• 69

  FuHZhuYWangYLiuZZhangJWangZet al. Tumor Infiltrating Mast Cells (TIMs) Confers a Marked Survival Advantage in Nonmetastatic Clear-Cell Renal Cell Carcinoma. Ann Surg Oncol (2017) 24(5):1435–42. doi: 10.1245/s10434-016-5702-5

  • CrossRef
  • Google Scholar

• 70

  HedstromGBerglundMMolinDFischerMNilssonGThunbergUet al. Mast Cell Infiltration Is a Favourable Prognostic Factor in Diffuse Large B-Cell Lymphoma. Br J Haematol (2007) 138(1):68–71. doi: 10.1111/j.1365-2141.2007.06612.x

  • CrossRef
  • Google Scholar

• 71

  YangXDAiWAsfahaSBhagatGFriedmanRAJinGet al. Histamine Deficiency Promotes Inflammation-Associated Carcinogenesis Through Reduced Myeloid Maturation and Accumulation of CD11b+Ly6G+ Immature Myeloid Cells. Nat Med (2011) 17(1):87–95. doi: 10.1038/nm.2278

  • CrossRef
  • Google Scholar

• 72

  SiebenhaarFMetzMMaurerM. Mast Cells Protect From Skin Tumor Development and Limit Tumor Growth During Cutaneous De Novo Carcinogenesis in a Kit-Dependent Mouse Model. Exp Dermatol (2014) 23(3):159–64. doi: 10.1111/exd.12328

  • CrossRef
  • Google Scholar

• 73

  CherdantsevaTMBobrovIPAvdalyanAMKlimachevVVKazartsevAVKryuchkovaNGet al. Mast Cells in Renal Cancer: Clinical Morphological Correlations and Prognosis. Bull Exp Biol Med (2017) 163(6):801–4. doi: 10.1007/s10517-017-3907-7

  • CrossRef
  • Google Scholar

• 74

  DantasRCMde SouzaROValverdeLFVidalMTASalesCBSSousaLPet al. Evaluation of Mast Cell Density in the Tumor Microenvironment in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma. Appl Immunohistochem Mol Morphol (2017) 25(10):e83–8. doi: 10.1097/PAI.0000000000000587

  • CrossRef
  • Google Scholar

• 75

  BrockmeyerPKlingASchulzXPerskeCSchliephakeHHemmerleinB. High Mast Cell Density Indicates a Longer Overall Survival in Oral Squamous Cell Carcinoma. Sci Rep (2017) 7(1):14677. doi: 10.1038/s41598-017-15406-5

  • CrossRef
  • Google Scholar

• 76

  WelshTJGreenRHRichardsonDWallerDAO’ByrneKJBraddingP. Macrophage and Mast-Cell Invasion of Tumor Cell Islets Confers a Marked Survival Advantage in Non-Small-Cell Lung Cancer. J Clin Oncol (2005) 23(35):8959–67. doi: 10.1200/JCO.2005.01.4910

  • CrossRef
  • Google Scholar

• 77

  ShikotraAOhriCMGreenRHWallerDABraddingP. Mast Cell Phenotype, TNFalpha Expression and Degranulation Status in Non-Small Cell Lung Cancer. Sci Rep (2016) 6:38352. doi: 10.1038/srep38352

  • CrossRef
  • Google Scholar

• 78

  SinnamonMJCarterKJSimsLPLafleurBFingletonBMatrisianLM. A Protective Role of Mast Cells in Intestinal Tumorigenesis. Carcinogenesis (2008) 29(4):880–6. doi: 10.1093/carcin/bgn040

  • CrossRef
  • Google Scholar

• 79

  CarliniMJDalurzoMCLastiriJMSmithDEVasalloBCPuricelliLIet al. Mast Cell Phenotypes and Microvessels in Non-Small Cell Lung Cancer and Its Prognostic Significance. Hum Pathol (2010) 41(5):697–705. doi: 10.1016/j.humpath.2009.04.029

  • CrossRef
  • Google Scholar

• 80

  OldfordSAHaidlIDHowattMALeivaCAJohnstonBMarshallJS. A Critical Role for Mast Cells and Mast Cell-Derived IL-6 in TLR2-Mediated Inhibition of Tumor Growth. J Immunol (2010) 185(11):7067–76. doi: 10.4049/jimmunol.1001137

  • CrossRef
  • Google Scholar

• 81

  PurwarRSchlapbachCXiaoSKangHSElyamanWJiangXet al. Robust Tumor Immunity to Melanoma Mediated by Interleukin-9-Producing T Cells. Nat Med (2012) 18(8):1248–53. doi: 10.1038/nm.2856

  • CrossRef
  • Google Scholar

• 82

  Rabelo MeloFSantosh MartinSSommerhoffCPPejlerG. Exosome-Mediated Uptake of Mast Cell Tryptase Into the Nucleus of Melanoma Cells: A Novel Axis for Regulating Tumor Cell Proliferation and Gene Expression. Cell Death Dis (2019) 10(9):659. doi: 10.1038/s41419-019-1879-4

  • CrossRef
  • Google Scholar

• 83

  FleischmannASchlommTKollermannJSekulicNHulandHMirlacherMet al. Immunological Microenvironment in Prostate Cancer: High Mast Cell Densities Are Associated With Favorable Tumor Characteristics and Good Prognosis. Prostate (2009) 69(9):976–81. doi: 10.1002/pros.20948

  • CrossRef
  • Google Scholar

• 84

  ForoozanMRoudiRAbolhasaniMGheytanchiEMehrazmaM. Clinical Significance of Endothelial Cell Marker CD34 and Mast Cell Marker CD117 in Prostate Adenocarcinoma. Pathol Res Pract (2017) 213(6):612–8. doi: 10.1016/j.prp.2017.04.027

  • CrossRef
  • Google Scholar

• 85

  HempelHACukaNSKulacIBarberJRCornishTCPlatzEAet al. Low Intratumoral Mast Cells Are Associated With a Higher Risk of Prostate Cancer Recurrence. Prostate (2017) 77(4):412–24. doi: 10.1002/pros.23280

  • CrossRef
  • Google Scholar

• 86

  TanSYFanYLuoHSShenZXGuoYZhaoLJ. Prognostic Significance of Cell Infiltrations of Immunosurveillance in Colorectal Cancer. World J Gastroenterol (2005) 11(8):1210–4. doi: 10.3748/wjg.v11.i8.1210

  • CrossRef
  • Google Scholar

• 87

  NaikRBaligaPBansalRPaiM. Distribution of Mast Cells in the Axillary Lymph Nodes of Breast Cancer Patients. J Indian Med Assoc (1997) 95(12):606–7.

  • Google Scholar

• 88

  DabiriSHuntsmanDMakretsovNCheangMGilksBBajdikCet al. The Presence of Stromal Mast Cells Identifies a Subset of Invasive Breast Cancers With a Favorable Prognosis. Mod Pathol (2004) 17(6):690–5. doi: 10.1038/modpathol.3800094

  • CrossRef
  • Google Scholar

• 89

  RibattiDFinatoNCrivellatoEGuidolinDLongoVMangieriDet al. Angiogenesis and Mast Cells in Human Breast Cancer Sentinel Lymph Nodes With and Without Micrometastases. Histopathology (2007) 51(6):837–42. doi: 10.1111/j.1365-2559.2007.02869.x

  • CrossRef
  • Google Scholar

• 90

  RajputABTurbinDACheangMCVoducDKLeungSGelmonKAet al. Stromal Mast Cells in Invasive Breast Cancer Are a Marker of Favourable Prognosis: A Study of 4,444 Cases. Breast Cancer Res Treat (2008) 107(2):249–57. doi: 10.1007/s10549-007-9546-3

  • CrossRef
  • Google Scholar

• 91

  AminiRMAaltonenKNevanlinnaHCarvalhoRSalonenLHeikkilaPet al. Mast Cells and Eosinophils in Invasive Breast Carcinoma. BMC Cancer (2007) 7:165. doi: 10.1186/1471-2407-7-165

  • CrossRef
  • Google Scholar

• 92

  della RovereFGranataAFamiliariDD’ArrigoGMondelloBBasileG. Mast Cells in Invasive Ductal Breast Cancer: Different Behavior in High and Minimum Hormone-Receptive Cancers. Anticancer Res (2007) 27(4B):2465–71.

  • Google Scholar

• 93

  MercoglianoMFBruniSMauroFElizaldePVSchillaciR. Harnessing Tumor Necrosis Factor Alpha to Achieve Effective Cancer Immunotherapy. Cancers (Basel) (2021) 13(3):564. doi: 10.3390/cancers13030564

  • CrossRef
  • Google Scholar

• 94

  NicoudMBSterleHAMassariNATaquez DelgadoMAFormosoKHerrero DuclouxMVet al. Study of the Antitumour Effects and the Modulation of Immune Response by Histamine in Breast Cancer. Br J Cancer (2020) 122(3):348–60. doi: 10.1038/s41416-019-0636-x

  • CrossRef
  • Google Scholar

• 95

  YanWLShenKYTienCYChenYALiuSJ. Recent Progress in GM-CSF-Based Cancer Immunotherapy. Immunotherapy (2017) 9(4):347–60. doi: 10.2217/imt-2016-0141

  • CrossRef
  • Google Scholar

• 96

  TaskinenMKarjalainen-LindsbergMLLeppaS. Prognostic Influence of Tumor-Infiltrating Mast Cells in Patients With Follicular Lymphoma Treated With Rituximab and CHOP. Blood (2008) 111(9):4664–7. doi: 10.1182/blood-2007-11-125823

  • CrossRef
  • Google Scholar

• 97

  MolinDEdstromAGlimeliusIGlimeliusBNilssonGSundstromCet al. Mast Cell Infiltration Correlates With Poor Prognosis in Hodgkin’s Lymphoma. Br J Haematol (2002) 119(1):122–4. doi: 10.1046/j.1365-2141.2002.03768.x

  • CrossRef
  • Google Scholar

• 98

  EnglundAMolinDEnbladGKarlenJGlimeliusILjungmanGet al. The Role of Tumour-Infiltrating Eosinophils, Mast Cells and Macrophages in Classical and Nodular Lymphocyte Predominant Hodgkin Lymphoma in Children. Eur J Haematol (2016) 97(5):430–8. doi: 10.1111/ejh.12747

  • CrossRef
  • Google Scholar

• 99

  AndersenMDKamperPNielsenPSBendixKRiber-HansenRSteinicheTet al. Tumour-Associated Mast Cells in Classical Hodgkin’s Lymphoma: Correlation With Histological Subtype, Other Tumour-Infiltrating Inflammatory Cell Subsets and Outcome. Eur J Haematol (2016) 96(3):252–9. doi: 10.1111/ejh.12583

  • CrossRef
  • Google Scholar

• 100

  BanatGATretynAPullamsettiSSWilhelmJWeigertAOleschCet al. Immune and Inflammatory Cell Composition of Human Lung Cancer Stroma. PLoS One (2015) 10(9):e0139073. doi: 10.1371/journal.pone.0139073

  • CrossRef
  • Google Scholar

• 101

  GiannouADMaraziotiASpellaMKanellakisNIApostolopoulouHPsallidasIet al. Mast Cells Mediate Malignant Pleural Effusion Formation. J Clin Invest (2015) 125(6):2317–34. doi: 10.1172/JCI79840

  • CrossRef
  • Google Scholar

• 102

  HolzelMLandsbergJGloddeNBaldTRogavaMRiesenbergSet al. A Preclinical Model of Malignant Peripheral Nerve Sheath Tumor-Like Melanoma Is Characterized by Infiltrating Mast Cells. Cancer Res (2016) 76(2):251–63. doi: 10.1158/0008-5472.CAN-15-1090

  • CrossRef
  • Google Scholar

• 103

  MaltbySKhazaieKMcNagnyKM. Mast Cells in Tumor Growth: Angiogenesis, Tissue Remodelling and Immune-Modulation. Biochim Biophys Acta (2009) 1796(1):19–26. doi: 10.1016/j.bbcan.2009.02.001

  • CrossRef
  • Google Scholar

• 104

  RibattiDCrivellatoE. Mast Cells, Angiogenesis, and Tumour Growth. Biochim Biophys Acta (2012) 1822(1):2–8. doi: 10.1016/j.bbadis.2010.11.010

  • CrossRef
  • Google Scholar

• 105

  KomiDEARedegeldFA. Role of Mast Cells in Shaping the Tumor Microenvironment. Clin Rev Allergy Immunol (2020) 58(3):313–25. doi: 10.1007/s12016-019-08753-w

  • CrossRef
  • Google Scholar

• 106

  RanieriGAmmendolaMPatrunoRCelanoGZitoFAMontemurroSet al. Tryptase-Positive Mast Cells Correlate With Angiogenesis in Early Breast Cancer Patients. Int J Oncol (2009) 35(1):115–20. doi: 10.3892/ijo_00000319

  • CrossRef
  • Google Scholar

• 107

  Aponte-LopezAFuentes-PananaEMCortes-MunozDMunoz-CruzS. Mast Cell, the Neglected Member of the Tumor Microenvironment: Role in Breast Cancer. J Immunol Res (2018) 2018:2584243. doi: 10.1155/2018/2584243

  • CrossRef
  • Google Scholar

• 108

  AmmendolaMSaccoRSammarcoGLuposellaMPatrunoRGadaletaCDet al. Mast Cell-Targeted Strategies in Cancer Therapy. Transfus Med Hemother (2016) 43(2):109–13. doi: 10.1159/000444942

  • CrossRef
  • Google Scholar

• 109

  RigoniAColomboMPPucilloC. The Role of Mast Cells in Molding the Tumor Microenvironment. Cancer Microenviron (2015) 8(3):167–76. doi: 10.1007/s12307-014-0152-8

  • CrossRef
  • Google Scholar

• 110

  PiliponskyAMChenC-CGrimbaldestonMABurns-GuydishSMHardyJKalesnikoffJet al. Mast Cell-Derived TNF can Exacerbate Mortality During Severe Bacterial Infections in C57BL/6-KitW-Sh/W-Sh Mice. Am J Pathol (2010) 176(2):926–38. doi: 10.2353/ajpath.2010.090342

  • CrossRef
  • Google Scholar

• 111

  RodewaldHRFeyerabendTB. Widespread Immunological Functions of Mast Cells: Fact or Fiction? Immunity (2012) 37(1):13–24. doi: 10.1016/j.immuni.2012.07.007

  • CrossRef
  • Google Scholar

• 112

  KatzHRAustenKF. Mast Cell Deficiency, a Game of Kit and Mouse. Immunity (2011) 35(5):668–70. doi: 10.1016/j.immuni.2011.11.004

  • CrossRef
  • Google Scholar

• 113

  RibattiD. Mast Cells in Lymphomas. Crit Rev Oncol Hematol (2016) 101:207–12. doi: 10.1016/j.critrevonc.2016.03.016

  • CrossRef
  • Google Scholar

• 114

  AaltomaaSLipponenPPapinahoSKosmaVM. Mast Cells in Breast Cancer. Anticancer Res (1993) 13:785–8.

  • Google Scholar

• 115

  MestasJHughesCC. Of Mice and Not Men: Differences Between Mouse and Human Immunology. J Immunol (2004) 172(5):2731–8. doi: 10.4049/jimmunol.172.5.2731

  • CrossRef
  • Google Scholar

• 116

  PassanteE. Mast Cell and Basophil Cell Lines: A Compendium. Methods Mol Biol (2014) 1192:101–13. doi: 10.1007/978-1-4939-1173-8_8

  • CrossRef
  • Google Scholar

• 117

  EpsteinMM. Do Mouse Models of Allergic Asthma Mimic Clinical Disease? Int Arch Allergy Immunol (2004) 133(1):84–100. doi: 10.1159/000076131

  • CrossRef
  • Google Scholar

• 118

  FinkelmanFD. Anaphylaxis: Lessons From Mouse Models. JAllergy Clin Immunol (2007) 120(3):506–15. doi: 10.1016/j.jaci.2007.07.033

  • CrossRef
  • Google Scholar

• 119

  MaurerMTaubeCSchroderNWJEbmeyerJSiebenhaarFGeldmacherAet al. Mast Cells Drive IgE-Mediated Disease But may be Bystanders in Many Other Inflammatory and Neoplastic Conditions. J Allergy Clin Immunol (2019) 144(4):S19–30. doi: 10.1016/j.jaci.2019.07.017

  • CrossRef
  • Google Scholar

• 120

  BischoffSC. Role of Mast Cells in Allergic and Non-Allergic Immune Responses: Comparison of Human and Murine Data. Nat Rev Immunol (2007) 7(2):93–104. doi: 10.1038/nri2018

  • CrossRef
  • Google Scholar

• 121

  ZschalerJSchlorkeDArnholdJ. Differences in Innate Immune Response Between Man and Mouse. Crit Rev Immunol (2014) 34(5):433–54. doi: 10.1615/CritRevImmunol.2014011600

  • CrossRef
  • Google Scholar

• 122

  GalliSJTsaiMPiliponskyAM. The Development of Allergic Inflammation. Nature (2008) 454(7203):445–54. doi: 10.1038/nature07204

  • CrossRef
  • Google Scholar

• 123

  AkulaSPaivandyAFuZThorpeMPejlerGHellmanL. How Relevant Are Bone Marrow-Derived Mast Cells (BMMCs) as Models for Tissue Mast Cells? A Comparative Transcriptome Analysis of BMMCs and Peritoneal Mast Cells. Cells (2020) 9(9):2118. doi: 10.3390/cells9092118

  • CrossRef
  • Google Scholar

• 124

  ZhaoWKepleyCLMorelPAOkumotoLMFukuokaYSchwartzLB. Fc Gamma RIIa, Not Fc Gamma RIIb, Is Constitutively and Functionally Expressed on Skin-Derived Human Mast Cells. J Immunol (2006) 177(1):694–701. doi: 10.4049/jimmunol.177.1.694

  • CrossRef
  • Google Scholar

• 125

  YuYBlokhuisBRGarssenJRedegeldFA. Non-IgE Mediated Mast Cell Activation. Eur J Pharmacol (2016) 778:33–43. doi: 10.1016/j.ejphar.2015.07.017

  • CrossRef
  • Google Scholar

• 126

  BruhnsP. Properties of Mouse and Human IgG Receptors and Their Contribution to Disease Models. Blood (2012) 119(24):5640–9. doi: 10.1182/blood-2012-01-380121

  • CrossRef
  • Google Scholar

• 127

  XingWAustenKFGurishMFJonesTG. Protease Phenotype of Constitutive Connective Tissue and of Induced Mucosal Mast Cells in Mice Is Regulated by the Tissue. Proc Natl Acad Sci USA (2011) 108(34):14210–5. doi: 10.1073/pnas.1111048108

  • CrossRef
  • Google Scholar

• 128

  DaiHKorthuisRJ. Mast Cell Proteases and Inflammation. Drug Discovery Today Dis Models (2011) 8(1):47–55. doi: 10.1016/j.ddmod.2011.06.004

  • CrossRef
  • Google Scholar

• 129

  ChauhanJMcCrawAJNakamuraMOsbornGSowHSCoxVFet al. IgE Antibodies Against Cancer: Efficacy and Safety. Antibodies (2020) 9(4):55. doi: 10.3390/antib9040055

  • CrossRef
  • Google Scholar

• 130

  PellizzariGBaxHJJosephsDHGotovinaJJensen-JarolimESpicerJFet al. Harnessing Therapeutic IgE Antibodies to Re-Educate Macrophages Against Cancer. Trends Mol Med (2020) 26(6):615–26. doi: 10.1016/j.molmed.2020.03.002

  • CrossRef
  • Google Scholar

• 131

  TaniguchiSElhanceAVan DuzerAKumarSLeitenbergerJJOshimoriN. Tumor-Initiating Cells Establish an IL-33–TGF-β Niche Signaling Loop to Promote Cancer Progression. Science (2020) 369(6501):eaay1813. doi: 10.1126/science.aay1813

  • CrossRef
  • Google Scholar

• 132

  KamphuisJBJWorrallWPMStackowiczJMougelAMauréECondeEet al. Comment on “Tumor-Initiating Cells Establish an IL-33-TGF-β Niche Signaling Loop to Promote Cancer Progression”. Science (2021) 372(6538):eabf2022. doi: 10.1126/science.abf2022

  • CrossRef
  • Google Scholar

• 133

  DuanZLuoY. Targeting Macrophages in Cancer Immunotherapy. Signal Transduct Target Ther (2021) 6(1):127. doi: 10.1038/s41392-021-00506-6

  • CrossRef
  • Google Scholar

• 134

  CotechiniTAtallahAGrossmanA. Tissue-Resident and Recruited Macrophages in Primary Tumor and Metastatic Microenvironments: Potential Targets in Cancer Therapy. Cells (2021) 10(4):960. doi: 10.3390/cells10040960

  • CrossRef
  • Google Scholar

• 135

  MehrajUQayoomHMirMA. Prognostic Significance and Targeting Tumor-Associated Macrophages in Cancer: New Insights and Future Perspectives. Breast Cancer (2021) 28(3):539–55. doi: 10.1007/s12282-021-01231-2

  • CrossRef
  • Google Scholar

• 136

  SihraBSKonOMGrantJAKayAB. Expression of High-Affinity IgE Receptors (FcϵRI) on Peripheral Blood Basophils, Monocytes, and Eosinophils in Atopic and Nonatopic Subjects: Relationship to Total Serum IgE Concentrations. J Allergy Clin Immunol (1997) 99(5):699–706. doi: 10.1016/S0091-6749(97)70033-2

  • CrossRef
  • Google Scholar

• 137

  MaurerDFiebigerEReiningerBWolff-WiniskiBJouvinMHKilgusOet al. Expression of Functional High Affinity Immunoglobulin E Receptors (Fc Epsilon RI) on Monocytes of Atopic Individuals. J Exp Med (1994) 179(2):745–50. doi: 10.1084/jem.179.2.745

  • CrossRef
  • Google Scholar

• 138

  GinhouxFJungS. Monocytes and Macrophages: Developmental Pathways and Tissue Homeostasis. Nat Rev Immunol (2014) 14(6):392–404. doi: 10.1038/nri3671

  • CrossRef
  • Google Scholar

• 139

  BannertCBidmon-FliegenschneeBStaryGHotzyFStiftJNurkoSet al. Fc-Epsilon-RI, the High Affinity IgE-Receptor, Is Robustly Expressed in the Upper Gastrointestinal Tract and Modulated by Mucosal Inflammation. PLoS One (2012) 7(7):e42066. doi: 10.1371/journal.pone.0042066

  • CrossRef
  • Google Scholar

• 140

  MacDermotJJosephSDolleryCT. The Identity of IgE Receptors (Fc Epsilon RII) That Mediate Cellular Activation of Human Macrophages: Evidence Against a Role for CD23. Mol Immunol (1992) 29(1):71–6. doi: 10.1016/0161-5890(92)90158-T

  • CrossRef
  • Google Scholar

• 141

  OsterhoffBRappersbergerKWangBKoszikFOchiaiKKinetJPet al. Immunomorphologic Characterization of Fc Epsilon RI-Bearing Cells Within the Human Dermis. J Invest Dermatol (1994) 102(3):315–20. doi: 10.1111/1523-1747.ep12371789

  • CrossRef
  • Google Scholar

• 142

  EscribeseMMRosaceDChivatoTFernandezTDCorbiALBarberD. Alternative Anaphylactic Routes: The Potential Role of Macrophages. Front Immunol (2017) 8:515. doi: 10.3389/fimmu.2017.00515

  • CrossRef
  • Google Scholar

• 143

  FinkelmanFDKhodounMVStraitR. Human IgE-Independent Systemic Anaphylaxis. J Allergy Clin Immunol (2016) 137(6):1674–80. doi: 10.1016/j.jaci.2016.02.015

  • CrossRef
  • Google Scholar

• 144

  JosephMTonnelABCapronADessaintJP. The Interaction of IgE Antibody With Human Alveolar Macrophages and Its Participation in the Inflammatory Processes of Lung Allergy. Agents Actions (1981) 11(6-7):619–22. doi: 10.1007/BF01978766

  • CrossRef
  • Google Scholar

• 145

  JosephMTonnelABTorpierGCapronAArnouxBBenvenisteJ. Involvement of Immunoglobulin E in the Secretory Processes of Alveolar Macrophages From Asthmatic Patients. J Clin Invest (1983) 71(2):221–30. doi: 10.1172/JCI110762

  • CrossRef
  • Google Scholar

• 146

  CapronMJouaultTPrinLJosephMAmeisenJCButterworthAEet al. Functional Study of a Monoclonal Antibody to IgE Fc Receptor (Fc Epsilon R2) of Eosinophils, Platelets, and Macrophages. J Exp Med (1986) 164(1):72–89. doi: 10.1084/jem.164.1.72

  • CrossRef
  • Google Scholar

• 147

  MelewiczFMPlummerJMSpiegelbergHL. Comparison of the Fc Receptors for IgE on Human Lymphocytes and Monocytes. J Immunol (1982) 129(2):563–9.

  • Google Scholar

• 148

  SpiegelbergHLBoltz-NitulescuGPlummerJMMelewiczFM. Characterization of the IgE Fc Receptors on Monocytes and Macrophages. Fed Proc (1983) 42(1):124–8.

  • Google Scholar

• 149

  NoroNYoshiokaAAdachiMYasudaKMasudaTYodoiJ. Monoclonal Antibody (H107) Inhibiting IgE Binding to Fc Epsilon R(+) Human Lymphocytes. J Immunol (1986) 137(4):1258–63.

  • Google Scholar

• 150

  KinetJP. The High-Affinity IgE Receptor (Fc Epsilon RI): From Physiology to Pathology. AnnuRevImmunol (1999) 17:931–72. doi: 10.1146/annurev.immunol.17.1.931

  • CrossRef
  • Google Scholar

• 151

  IraniAMHuangCXiaHZKepleyCNafieAFoudaEDet al. Immunohistochemical Detection of Human Basophils in Late-Phase Skin Reactions. J Allergy Clin Immunol (1998) 101(3):354–62. doi: 10.1016/S0091-6749(98)70248-9

  • CrossRef
  • Google Scholar

• 152

  KitaHGleichGJ. Eosinophils and IgE Receptors: A Continuing Controversy. Blood (1997) 89(10):3497–501. doi: 10.1182/blood.V89.10.3497

  • CrossRef
  • Google Scholar

• 153

  IshizukaTOkayamaYKobayashiHMoriM. Interleukin-10 Is Localized to and Released by Human Lung Mast Cells. Clin Exp Allergy (1999) 29(10):1424–32. doi: 10.1046/j.1365-2222.1999.00636.x

  • CrossRef
  • Google Scholar

• 154

  BaghestanianMHofbauerRKienerHPBanklHCWimazalFWillheimMet al. The C-Kit Ligand Stem Cell Factor and Anti-IgE Promote Expression of Monocyte Chemoattractant Protein-1 in Human Lung Mast Cells. Blood (1997) 90(11):4438–49. doi: 10.1182/blood.V90.11.4438.4438_4438_4449

  • CrossRef
  • Google Scholar

• 155

  da SilvaEZJamurMCOliverC. Mast Cell Function: A New Vision of an Old Cell. J Histochem Cytochem (2014) 62(10):698–738. doi: 10.1369/0022155414545334

  • CrossRef
  • Google Scholar

• 156

  CasaleTBWoodDRichersonHBZehrBZavalaDHunninghakeGW. Direct Evidence of a Role for Mast Cells in the Pathogenesis of Antigen-Induced Bronchoconstriction. J Clin Invest (1987) 80(5):1507–11. doi: 10.1172/JCI113234

  • CrossRef
  • Google Scholar

• 157

  MacielTTMouraICHermineO. The Role of Mast Cells in Cancers. F1000Prime Rep (2015) 7:09. doi: 10.12703/P7-09

  • CrossRef
  • Google Scholar

• 158

  SchleimerRPMacGlashanDWJr.SchulmanESPetersSPAdamsGKAdkinsonNFJr.et al. Human Mast Cells and Basophils–Structure, Function, Pharmacology, and Biochemistry. ClinRevAllergy (1983) 1:327–41. doi: 10.1007/BF02991224

  • CrossRef
  • Google Scholar

• 159

  TurnerHKinetJP. Signalling Through the High-Affinity IgE Receptor Fc epsilonRI. Nature (1999) 402(6760 Suppl):B24–30. doi: 10.1038/35037021

  • CrossRef
  • Google Scholar

• 160

  SaxenaSSinghASinghP. Tumor Associated Mast Cells: Biological Roles and Therapeutic Applications. Anat Cell Biol (2020) 53(3):245–51. doi: 10.5115/acb.19.181

  • CrossRef
  • Google Scholar

• 161

  GouldHJSuttonBJ. IgE in Allergy and Asthma Today. Nat Rev Immunol (2008) 8(3):205–17. doi: 10.1038/nri2273

  • CrossRef
  • Google Scholar

• 162

  FolkertsJGaudenzioNMaurerMHendriksRWStadhoudersRTamSYet al. Rapid Identification of Human Mast Cell Degranulation Regulators Using Functional Genomics Coupled to High-Resolution Confocal Microscopy. Nat Protoc (2020) 15(3):1285–310. doi: 10.1038/s41596-019-0288-6

  • CrossRef
  • Google Scholar

• 163

  Dela CruzJSTrinhKRMorrisonSLPenichetML. Recombinant Anti-Human HER2/neu IgG3-(GM-CSF) Fusion Protein Retains Antigen Specificity and Cytokine Function and Demonstrates Antitumor Activity. J Immunol (2000) 165(9):5112–21. doi: 10.4049/jimmunol.165.9.5112

  • CrossRef
  • Google Scholar

• 164

  ShiauMYChiouHLLeeYLKuoTM. & Chang, Y. H.: Establishment of a Consistent L929 Bioassay System for TNF-α Quantitation to Evaluate the Effect of Lipopolysaccharide, Phytomitogens and Cytodifferentiation Agents on Cytotoxicity of TNF-α Secreted by Adherent Human Mononuclear Cells. Mediators Inflamm (2001) 10(4):199–208. doi: 10.1080/09629350123139

  • CrossRef
  • Google Scholar

• 165

  ZanataFShaikSDevireddyRVWuXFerreiraLMGimbleJM. Cryopreserved Adipose Tissue-Derived Stromal/Stem Cells: Potential for Applications in Clinic and Therapy. Adv Exp Med Biol (2016) 951:137–46. doi: 10.1007/978-3-319-45457-3_11

  • CrossRef
  • Google Scholar

• 166

  LimMHOngWKSugiiS. The Current Landscape of Adipose-Derived Stem Cells in Clinical Applications. Expert Rev Mol Med (2014) 16:e8. doi: 10.1017/erm.2014.8

  • CrossRef
  • Google Scholar

• 167

  ConradDHWingardJRIshizakaT. The Interaction of Human and Rodent IgE With the Human Basophil IgE Receptor. J Immunol (1983) 130(1):327–33.

  • Google Scholar

• 168

  AchatzG. The Biology of IgE: Molecular Mechanism Restraining Potentially Dangerous High Serum IgE Titres In Vivo. In: PenichetMJensen-JarolimE. editors. Cancer and IgE. (2010). doi: 10.1007/978-1-60761-451-7_2

  • CrossRef
  • Google Scholar

• 169

  KuboSNakayamaTMatsuokaKYonekawaHKarasuyamaH. Long Term Maintenance of IgE-Mediated Memory in Mast Cells in the Absence of Detectable Serum IgE. J Immunol (2003) 170(2):775–80. doi: 10.4049/jimmunol.170.2.775

  • CrossRef
  • Google Scholar

• 170

  LawrenceMGWoodfolkJASchuylerAJStillmanLCChapmanMDPlatts-MillsTAE. Half-Life of IgE in Serum and Skin: Consequences for Anti-IgE Therapy in Patients With Allergic Disease. J Allergy Clin Immunol (2017) 139(2):422–8.e424.

  • Google Scholar

• 171

  BeckLAMarcotteGVMacGlashanDTogiasASainiS. Omalizumab-Induced Reductions in Mast Cell Fcϵri Expression and Function. J Allergy Clin Immunol (2004) 114(3):527–30. doi: 10.1016/j.jaci.2004.06.032

  • CrossRef
  • Google Scholar

• 172

  CassRMAndersenBR. The Disappearance Rate of Skin-Sensitizing Antibody Activity After Intradermal Administration. J Allergy (1968) 42(1):29–35. doi: 10.1016/0021-8707(68)90129-9

  • CrossRef
  • Google Scholar

• 173

  OettgenHC. Fifty Years Later: Emerging Functions of IgE Antibodies in Host Defense, Immune Regulation, and Allergic Diseases. J Allergy Clin Immunol (2016) 137(6):1631–45. doi: 10.1016/j.jaci.2016.04.009

  • CrossRef
  • Google Scholar

• 174

  DellingerALCuninPLeeDKungALBrooksDBZhouZet al. Inhibition of Inflammatory Arthritis Using Fullerene Nanomaterials. PLoS One (2015) 10(4):e0126290. doi: 10.1371/journal.pone.0126290

  • CrossRef
  • Google Scholar

• 175

  NortonSKDellingerAZhouZLenkRMacfarlandDVonakisBet al. A New Class of Human Mast Cell and Peripheral Blood Basophil Stabilizers That Differentially Control Allergic Mediator Release. Clin Trans Sci (2010) 3(4):158–69. doi: 10.1111/j.1752-8062.2010.00212.x

  • CrossRef
  • Google Scholar

• 176

  de VriesVCWasiukABennettKABensonMJElguetaRWaldschmidtTJet al. Mast Cell Degranulation Breaks Peripheral Tolerance. Am J Transplant (2009) 9(10):2270–80. doi: 10.1111/j.1600-6143.2009.02755.x

  • CrossRef
  • Google Scholar

• 177

  BrudnoJNKochenderferJN. Toxicities of Chimeric Antigen Receptor T Cells: Recognition and Management. Blood (2016) 127(26):3321–30. doi: 10.1182/blood-2016-04-703751

  • CrossRef
  • Google Scholar

• 178

  FesnakADJuneCHLevineBL. Engineered T Cells: The Promise and Challenges of Cancer Immunotherapy. Nat Rev Cancer (2016) 16(9):566–81. doi: 10.1038/nrc.2016.97

  • CrossRef
  • Google Scholar

• 179

  Martin-AntonioBSuneGPerez-AmillLCastellaMUrbano-IspizuaA. Natural Killer Cells: Angels and Devils for Immunotherapy. Int J Mol Sci (2017) 18(9):1868. doi: 10.3390/ijms18091868

  • CrossRef
  • Google Scholar

• 180

  GoldS. Premedication Use in Preventing Acute Infliximab Infusion Reactions in Patients With Inflammatory Bowel Disease: A Single Center Cohort Study. Inflamm Bowel Dis (2017) 23(10):1882–9. doi: 10.1097/MIB.0000000000001189

  • CrossRef
  • Google Scholar

• 181

  ToumaW. Risk Factors for and Pre-Medications to Prevent Cetuximab-Induced Infusion Reactions in Patients With Squamous Cell Carcinoma of the Head and Neck. Oral Oncol (2014) 50(9):895–900. doi: 10.1016/j.oraloncology.2014.06.017

  • CrossRef
  • Google Scholar

• 182

  NorthcuttMAl-SubuABellaBElitsurY. Safety of Infliximab in Children With IBD: The Experience of an Academic Center in WV. West Va Med J (2014) 110(3):26–9.

  • Google Scholar

• 183

  SorokinP. Mylotarg™ Approved for Patients With CD33+ Acute Myeloid Leukemia. Clin J Oncol Nurs (2000) 4(6):279–80.

  • Google Scholar

• 184

  Jensen-JarolimEAchatzGTurnerMCKaragiannisSLegrandFCapronMet al. AllergoOncology: The Role of IgE-Mediated Allergy in Cancer. Allergy (2008) 63(10):1255–66. doi: 10.1111/j.1398-9995.2008.01768.x

  • CrossRef
  • Google Scholar

• 185

  AlmagroJCDaniels-WellsTRPerez-TapiaSMPenichetML. Progress and Challenges in the Design and Clinical Development of Antibodies for Cancer Therapy. Front Immunol (2017) 8:1751. doi: 10.3389/fimmu.2017.01751

  • CrossRef
  • Google Scholar

• 186

  RosenbaumL. Tragedy, Perseverance, and Chance - The Story of CAR-T Therapy. N Engl J Med (2017) 377(14):1313–5. doi: 10.1056/NEJMp1711886

  • CrossRef
  • Google Scholar

• 187

  SieglerELWangP. Preclinical Models in Chimeric Antigen Receptor-Engineered T-Cell Therapy. Hum Gene Ther (2018) 29(5):534–46. doi: 10.1089/hum.2017.243

  • CrossRef
  • Google Scholar

• 188

  PorterDLLevineBLKalosMBaggAJuneCH. Chimeric Antigen Receptor-Modified T Cells in Chronic Lymphoid Leukemia. N Engl J Med (2011) 365(8):725–33. doi: 10.1056/NEJMoa1103849

  • CrossRef
  • Google Scholar

• 189

  Shimabukuro-VornhagenA. Cytokine Release Syndrome. J Immunother Cancer (2018) 6(1):56. doi: 10.1186/s40425-018-0343-9

  • CrossRef
  • Google Scholar