Shimaa M. A. Sayed
Karsten Siems3
Christian Schmitz-Linneweber
Walter Luyten
Nadine Saul- 1Molecular Genetics Group, Institute of Biology, Faculty of Life Sciences, Humboldt University of Berlin, Berlin, Germany
- 2Botany and Microbiology Department, Faculty of Science, New Valley University, El-Kharga, Egypt
- 3AnalytiCon Discovery GmbH, Potsdam, Germany
- 4Biology Department, KU Leuven, Leuven, Belgium
To uncover potential anti-aging capacities of Traditional Chinese Medicine (TCM), the nematode Caenorhabditis elegans was used to investigate the effects of Eucommia ulmoides and Cuscuta chinensis extracts, selected by screening seven TCM extracts, on different healthspan parameters. Nematodes exposed to E. ulmoides and C. chinensis extracts, starting at the young adult stage, exhibited prolonged lifespan and increased survival after heat stress as well as upon exposure to the pathogenic bacterium Photorhabdus luminescens, whereby the survival benefits were monitored after stress initiation at different adult stages. However, only C. chinensis had the ability to enhance physical fitness: the swimming behavior and the pharyngeal pumping rate of C. elegans were improved at day 7 and especially at day 12 of adulthood. Finally, monitoring the red fluorescence of aged worms revealed that only C. chinensis extracts caused suppression of intestinal autofluorescence, a known marker of aging. The results underline the different modes of action of the tested plants extracts. E. ulmoides improved specifically the physiological fitness by increasing the survival probability of C. elegans after stress, while C. chinensis seems to be an overall healthspan enhancer, reflected in the suppressed autofluorescence, with beneficial effects on physical as well as physiological fitness. The C. chinensis effects may be hormetic: this is supported by increased gene expression of hsp-16.1 and by trend, also of hsp-12.6.
Introduction
Aging is a complicated biological process that is associated with loss of physiological (e.g. stress resistance, metabolic rate, immune status), cognitive, reproductive and physical (e.g. locomotion and muscle integrity) functions which finally leads to death (Fuellen et al., 2019; Kocsisova et al., 2019). Over the past three decades, great outcomes have been achieved by aging researchers in prolonging lifespan, at least in model organisms including yeast (Saccharomyces cerevisiae), worms (Caenorhabditis elegans), and flies (Drosophila melanogaster), using genetic manipulations or nutritional approaches (Kirkland and Peterson, 2009). In humans, the extension of healthspan lags behind the increase of life expectancy, thus, extension of lifespan is often accompanied by the extension of time spent in ill health and reduced overall fitness (Kunugi and Mohammed Ali, 2019). Therefore, healthspan, defined as the period of life in which individuals are active and free from chronic disease, is the new target of anti-aging efforts instead of lifespan (Tissenbaum, 2015; Luyten et al., 2016). Indeed, over the past 10 years, the articles indexed in PubMed with the term “healthspan” or “health span” in the title or abstract increased dramatically (Kaeberlein, 2018).
The World Health Organization (WHO) estimated that more than 80% of the world’s population depends on herbs for their basic health care requirements (Zhang et al., 2013). Among those, Traditional Chinese Medicine (TCM) is one of the most important phytomedicine systems, which has been used in Asian countries for thousands of years (Li et al., 2019b). On May 25th, 2019, the WHO issued the 11th revision of the International Statistical Classification of Diseases (ICD-11) (WHO, 2019) which now includes TCM as a recognized phytomedicinal approach. This reflects the contribution of TCM to the world’s healthcare, and also acknowledges the current needs for TCM (Lam et al., 2019). Now, scientific efforts are focusing on separating the harmful and placebo effects of TCM treatments from the truly beneficial effects (Break-with-Traditon, 2019). However, as discussed by Fung and Linn (2015), high quality clinical trials to determine TCM’s efficacy are still rare. More than 400 different herbs with almost 4,000 different formulas are listed in “The Encyclopedia of Traditional Chinese Medicine” (Xu et al., 2018a). Since clinical trials are expensive, protracted, and require substantial organizational efforts, the most promising TCM preparations need to be wisely chosen from that long list. The current study aimed to help with this selection process.
Cuscuta chinensis (C. chinensis) Lam. (Family: Convolvulaceae) is one of the most commonly used herbs in TCM. It contains several pharmaceutically active compounds such as phenolic compounds, polysaccharides, lignans, flavonoids and resin glycosides typical for the family Convolvulaceae (Donnapee et al., 2014; Sun et al., 2014; Wei et al., 2019a). C. chinensis has been reported to display anticancer, anti-apoptosis, antioxidant, anti-inflammatory, immunostimulatory, anti-osteoporotic, and hepatoprotective activities, and is also widely used in the clinical treatment of kidney yang-deficiency (Yang et al., 2011; Kang et al., 2014; Sun et al., 2014; Yang et al., 2017). Eucommia ulmoides (E. ulmoides) Oliv. (Family: Eucommiaceae) is a native tree in China (Wuyun et al., 2018), which has been used for centuries in clinical treatment according to ancient Chinese medicine books such as the Shen-nong’s Classic of Materia Medica and Compendium (Ding and Wang, 2015; Ye et al., 2019). The bark, roots, and leaves of E. ulmoides have been reported as a good source of highly potent medicinal compounds (Xing et al., 2019), e.g. iridoids and lignanes. Furthermore, the aqueous extracts of E. ulmoides bark or leaves are usually used as popular drinks in Japan, Korea, and China for hypertension treatment (Kwan et al., 2003). The antioxidant effects of E. ulmoides are attributed to numerous anti-oxidative flavonoids such as rutin, as well as chlorogenic-, ferulic, and caffeic acid (Xu et al., 2018b; Liu et al., 2018).
The nematode Caenorhabditis elegans (C. elegans) is a prominent model organism used in many research areas, including aging (Gruber et al., 2014; Son et al., 2019), due to numerous advantages, such as short generation time, easy and cheap maintenance, simple anatomy and completely sequenced genome (Hastings et al., 2019). Therefore, this model was used to test the hypothesis that the health-promoting effects of TCMs are based on their general anti-aging properties and are, thus, reflected in different healthspan parameters. For that purpose, heat and pathogenic stress resistance, different swim characteristics, pharyngeal pumping, body growth, fecundity, and intestinal autofluorescence were analyzed after treatment of C. elegans with TCM plant extracts.
This study focuses on the general health effects of C. chinensis and E. ulmoides, which were selected among seven tested TCM extracts in a preliminary screen using C. elegans. In addition to C. chinensis and E. ulmoides, the screening included plant preparations from the TCM plants Achyranthes bidentata Blume, Astragalus membranaceus var. mongholicus (Bunge), Ligustrum lucidum W. T.Aiton, Wolfiporia extensa (Peck) Ginns (syn. Poria cocos (Schw.) Wolf and Schisandra chinensis (Turcz.) Baill, all of which have been claimed to possess anti-aging properties.
Materials and Methods
Preparation of Extracts From TCM Used in This Study
Plants were selected by a certified TCM-specialist: Prof. Qingfei Liu (Tsinghua University, Beijing, China). They were acquired from Tong Ren Tang, Beijing (https://www.tongrentangcm.com/en/), and their identity was verified by Prof. Liu, who is trained to recognize Chinese herbs. Table 1 shows the tested organic extracts prepared by AnalytiCon Discovery GmbH (Potsdam, Germany). The extracts are stored at AnalytiCon Discovery GmbH under the batch number specified in Table 1. The dried TCM plants (20 g each) were extracted with a mixture of tbutyl-methyl ether (MTBE) and methanol (50:50, volume 75 ml) as well as with 100% methanol (volume 75 ml) at ambient temperature. Extraction was assisted by 15 min sonication and 2 h maceration. Both extracts of either plant were combined and dried at 45 °C by a continuous stream of air for 4 h. Thereafter, the samples were weighed and analyzed by HPLC-MS (for HPLC-MS methods and results, see Supplementary Tables S1–S3 as well as Supplementary Figures S1–S7 in the Supplementary Materials). The LC-MS chromatograms qualitatively describe the extracts, but it is not yet possible to annotate structures to the peaks.
TABLE 1. Traditional Chinese Medicine (TCM) extracts used in this study.
C. elegans Maintenance
The wild-type C. elegans strain N2 (Bristol) as well as the Escherichia coli feeding strain OP50 were obtained from the Caenorhabditis Genetics Center (CGC) (Minneapolis, MN, United States). Nematodes were maintained according to Brenner (1974) at 22 °C on 96 mm nematode growth medium (NGM) agar plates seeded with live OP50 bacteria, which were grown at 37 °C and concentrated to OD595 = 5 beforehand. Synchronized and contamination-free populations were regularly generated by dissolving young adults in a 3% sodium hypochlorite solution until eggs were isolated, based on a protocol from Stiernagle (2006). The obtained eggs hatched in M9 buffer overnight, and were transferred to new NGM plates the following day.
TCMs Treatment of C. elegans
The plant extracts were dissolved in DMSO at a stock concentration of 60 mg/ml and then added to the NGM agar plates as well as the OP50 bacteria at a final concentration of 30 μg/ml, while DMSO (0.05%) was used as a control in all experiments. Synchronized, untreated L4 larvae were transferred to the prepared NGM (which included 2 mg/ml carbenicillin) agar plates, followed by the addition of 100 µM 5-fluorodeoxyuridine (FUdR), which prevents the development of progeny (Hosono, 1978). The nematodes were incubated on those plates at 22 °C until the respective adulthood stages, at which the following experiments were performed.
To test whether the TCMs show any antibacterial impact against E. coli OP50 or P. luminescens, the bacterial growth was determined by frequently measuring the optical density (at 595 nm) during exposure to 30 μg/ml of each TCM. The results were expressed as colony-forming units (CFU/ml) according to the method described by Peñuelas-Urquides et al. (2013).
Stress Resistance and Lifespan Assays
On the 3rd, 7th, and 12th day of adulthood, worms were exposed to heat stress at 37 °C for 3 h. Thereafter, the incubation at 22 °C was continued and the number of surviving nematodes was monitored daily to determine their heat stress resistance.
For the pathogenic stress resistance assay, Photorhabdus luminescens (subspec. Laumondii, strain TT01; obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany) was used as a known pathogenic bacterium for C. elegans (Sato et al., 2014). A frozen glycerol stock of P. luminescens bacteria was used to inoculate 50 ml LB Lennox medium. After growing in a shaker at 28 °C for 48 h and concentrating the bacteria to a final OD595 = 9, the agar plates containing P. luminescens were prepared according to Hoinville and Wollenberg (2018): NGM plates (35 mm diameter) were seeded with P. luminescens mixed with E. coli OP50 (1:1), and incubated for an additional 48 h at 28 °C. On the 3rd and 7th day of adulthood, worms were shifted to these modified NGM plates containing P. luminescens, incubated at 22 °C, and survivors were determined daily by observing their movement after a soft touch with a platinum wire under the microscope. Each stress assay was performed blinded and repeated independently at least two times.
To determine the effect of C. chinensis and E. ulmoides on the lifespan of C. elegans, L4-stage synchronized worms were transferred to small NGM agar plates (see TCMs Treatment of C. elegans). Surviving and dead worms were counted daily until all worms had died. In addition, 100 µM quercetin hydrate (Sigma Aldrich, St. Louis, MO, United States) was used as a positive control. Approximately 75–90 worms divided over three plates were used for each treatment, and the experiment was performed three times.
Pharyngeal Pumping Assay
The influence of C. chinensis and E. ulmoides on the pharyngeal pumping rate was measured on the 7th and 12th day of adulthood. About 50 worms of each exposure group and age were randomly selected and the pharyngeal pumping was determined during crawling on the OP50 seeded NGM agar by recording videos for 60 s using a digital microscope (VHX-600, Osaka, Japan) with ×500 magnification. Finally, the pumping was counted by visual inspection of the videos. The test was performed two times.
Measuring Food Intake by Using Fluorescent Bacteria
To study the effect of C. chinensis and E. ulmoides on the food intake of C. elegans, the E. coli strain OP50-GFP (CGC, Minneapolis, MN, United States) was used according to Raizen et al., (2012). Extract-treated and control worms were transferred on the 7th and 12th day of adulthood to small agar plates seeded with a confluent lawn of OP50-GFP, and were allowed to feed for 15 min. Thereafter, about 25–30 nematodes per age and treatment were rinsed, washed in M9 buffer, moved to a 2% agarose pad on a glass slide, and anesthetized by 1 M NaN3. The worms were photographed with an Axiolab fluorescence microscope (Carl Zeiss, Jena, Germany) equipped with a GFP filter and a ProgRes C12 digital camera (Jenoptik, Jena, Germany). Mean fluorescence intensities per single worm were quantified using the CellProfiler software (Mcquin et al., 2018). The intensity values were normalized by subtracting the green autofluorescence values measured in extract-treated and control worms of the same age, fed with standard OP50.
Intestinal Autofluorescence
The intestinal autofluorescence in extract-treated C. elegans was measured on the 7th and 12th days of adulthood according to Pincus et al., (2016). Approximately 25 individuals per age and treatment were mounted on a 2% agarose pad on a microscope glass slide, and immobilized using 1 M sodium azide. Red autofluorescence was determined and imaged using the Axiolab fluorescence microscope (Carl Zeiss, Jena, Germany) with a TRITC filter set (excitation: 546 nm; emission: 600 nm), and equipped with a ProgRes C12 digital camera (Jenoptik, Jena, Germany). Mean fluorescence intensities per nematode were quantified densitometrically using the CellProfiler software (Mcquin et al., 2018).
Swimming Behavior
Analysis of the swimming behavior of wild type C. elegans was carried out using the CeleST software (Restif et al., 2014). On the 7th and 12th day of adulthood, five worms each were transferred to wells with a depth of 0.5 mm and a diameter of 10 mm on a microscope slide, which were filled with M9 buffer and covered by a cover slip to facilitate visualization. Then, 60-s videos were recorded per well and ≥50 nematodes were used per treatment and age. After isolating every second frame of the videos, and applying the greyscale and invert mode via Adobe Photoshop, the wave initiation rate, activity index, brush stroke and body wave number were determined with the CeleST software.
Body Size and Fecundity Measurements
The size of treated and untreated worms was measured by transferring them on the 7th and 12th day of adulthood to a 2% agarose pad on a glass slide, and anesthetizing them by using 1 M sodium azide. The bright field image of each animal was captured using a microscope (Carl Zeiss, Jena, Germany) equipped with a digital camera (ProgRes C12, Jenoptik, Jena, Germany). The body size of at least 40 nematodes per treatment was measured by determining the number of covered pixels per body with the CellProfiler software.
The effect of C. chinensis and E. ulmoides on fecundity was determined by counting the daily and total offspring per nematode. Ten synchronized L4 hermaphrodites, which were treated with the tested plant extracts since L1 larval stage, were placed individually to treatment and control plates at 22 °C without the reproductive inhibitor FUdR. Every 24 h, each single worm was transferred to a fresh plate until day 3 of adulthood. The hatched eggs were allowed to develop to L2 or L3 larvae and then the total offspring produced by each individual was counted per day and finally summed up.
Quantitative Real-Time PCR
About 3,000 worms per sample were collected on ice on the 12th day of adulthood, washed with M9-buffer, transferred to lysis tubes containing ceramic beads and 100 µl Lysis Solution RL (innuSPEED Tissue RNA Kit, Analytik Jena, Germany) and then ground thoroughly in a homogenizer (speedMill plus, Analytik Jena, Germany). Total RNA was extracted according to the manufacturer’s protocol (innuSPEED Tissue RNA Kit, Analytik Jena, Germany) with subsequent DNAse treatment (TURBO DNA-free Kit, Invitrogen, Carlsbad, CA, United States). Gel-electrophoresis and NanoDrop measurements were used to determine the quality and quantity of the RNA samples. cDNA synthesis was performed with the Script cDNA Synthesis Kit (Jena Biosciences, Jena, Germany) using 1 µg of total RNA per sample. The Quantitative Real-Time PCR (qPCR) analysis was conducted with the Luna Universal qPCR Kit (New England BioLabs, Frankfurt a.M., Germany) and the MyiQ Single Color RT-PCR Detection System (Bio-Rad, Hercules, CA, United States). Each sample was measured in three biological replicates, and each biological replicate was measured in technical triplicates. The fold changes were calculated according to Pfaffl (2001) and act-1 and cdc-42 were used as reference genes for normalization. The primer sequences, annealing temperatures and efficiencies as well as the qPCR cycle protocol are given in Supplementary Tables S4, S5.
Statistical Analysis
Statistical analysis was carried out using the one-way ANOVA test followed by the Bonferroni’s multiple comparison test (https://astatsa.com/OneWay_Anova_with_TukeyHSD/) or a log-rank test via the Online Application for Survival analysis OASIS 2 (Han et al., 2016) with subsequent Bonferroni correction. Data are displayed as mean ± SEM (standard error of the mean). Changes were considered statistically significant if their p-value was *(p < 0.05), **(p < 0.01), ***(p < 0.001) or ****(p < 0.0001).
Results
E. ulmoides and C. chinensis Increased Heat Stress Resistance of C. elegans
To determine the effect of TCM extracts on heat stress resistance of C. elegans, extract-treated and control nematodes were exposed to a heat shock (37 °C for 3 h) at different ages of adulthood (3rd, 7th, and 12th day). Representative survival curves as well as mean survival following the heat stress condition are presented in Figure 1. The application of several extracts significantly increased the stress resistance of younger worms to heat shock. However, among the seven extracts, only E. ulmoides and C. chinensis were able to improve the survival in older (12-days) nematodes (Figures 1A,D). In contrast, the organic extract from Achyrantes bidentata was not able to provoke any changes in survival rates, neither in young nor in old nematodes.
FIGURE 1. Heat stress resistance of C. elegans in the presence of different TCM extracts. C. elegans was treated with seven different TCM plant preparations (30 μg/ml), and DMSO as control, starting at the L4 stage. Heat stress (37 °C for 3 h) was applied at different ages of adulthood (3rd, 7th and 12th day), and the survival after stress was monitored in the different treatment groups (A). The values represent the mean survival ± SEM from three biological replicates. Significant differences were determined by a log-rank test and Bonferroni correction, with *p < 0.05; **p < 0.01; ***p < 0.001 and ****p < 0.0001. In addition, representative survival curves are shown for nematodes treated with E. ulmoides and C. chinensis extracts, which were heat-stressed on the 3rd(B), 7th(C) and 12th(D) day of adulthood.
The mean survival of control animals after heat shock on the 3rd, 7th, and 12th day of adulthood amounted to 6.7, 5.2, and 4.0 days, respectively (Table 2), reflecting the general aging-dependence of the heat stress resistance parameter. The incubation with C. chinensis caused a survival increase at the aforementioned ages to 8.3, 6.2, and 4.6 days, respectively. Interestingly, application of E. ulmoides exhibited the strongest overall effect by boosting the nematodes’ survival to 9.3, 6.3 and 5.0 days, respectively. Figures 1B–D revealed that not only the mean lifespan was increased by these two extracts, but also the maximum lifespan (Table 2).
TABLE 2. Survival after heat stress in the presence and absence of TCM extracts.
E. ulmoides and C. chinensis Improved the Resistance to Photorhabdus luminescens
Photorhabdus luminescens is able to infect C. elegans and consequently increases the death rate (Sato et al., 2014; Hoinville and Wollenberg, 2018). In this study, the ability was investigated of TCM extracts to counteract the pathogen-induced mortality in C. elegans exposed to the P. luminescens strain TT01 starting on the 3rd and 7th day of adulthood. Since the ingestion of pathogens (essential for infection) cannot be guaranteed in old nematodes due to the drastically decreased pharyngeal pumping frequency, no pathogen resistance assay was started on the 12th day of adulthood.
As expected, the pathogen sensitivity increases with age, underlining the suitability of pathogen stress assays in aging research (Figure 2A). All extracts were able to prolong the survival during pathogen exposure when starting on the 3rd day of adulthood (Figure 2A). However, only E. ulmoides and C. chinensis caused in addition a survival advantage in middle-aged nematodes (Figures 2A–C). In detail, E. ulmoides and C. chinensis could prolong the mean survival of C. elegans infected on the 3rd day of adulthood to 10.3 and 10.5 days, respectively, compared to 8.6 days for untreated worms. For nematodes infected on the 7th day of adulthood, E. ulmoides and C. chinensis extended the mean survival by 26 and 16%, respectively, but only E. ulmoides was able to prolong the maximum lifespan in both assays (Table 3). To exclude simple pathogen-inhibiting effects by the extracts as the underlying reason for increased resistance, the growth of liquid P. luminescens cultures was observed during extract treatment (30 μg/ml). All treated cultures were able to grow in the same manner as the control (no added substance) or the DMSO solvent control (Figure 2D). Thus, antibacterial effects did not play a role in the observed pathogenic resistance. Moreover, the tested extracts also had no effect on the growth of OP50 (Figure 2E).
FIGURE 2. Survival of C. elegans during exposure to the pathogenic bacterium P. luminescens.C. elegans was treated with seven different TCM plant preparations (30 μg/ml), and DMSO as control, starting at the L4 stage (A) Pathogenic stress due to P. luminescens exposure was initiated on the 3rd or 7th day of adulthood, and the survival during stress was monitored in the different treatment groups. The values represent the mean survival ± SEM from three biological replicates. Significant differences were determined by log-rank test and Bonferroni correction with *p < 0.05; **p < 0.01; ***p < 0.001 or ****p < 0.0001. In addition, survival curves are shown for nematodes treated with E. ulmoides and C. chinensis extracts, which were exposed to the pathogen starting on the 3rd(B) and 7th(C) day of adulthood (D) The growth of P. luminescens and (E)E. coli OP50 was monitored during extract exposure and the colony-forming units per time point are shown.
TABLE 3. Nematode survival during P. luminescens exposure in the presence and absence of TCM extracts.
Based on their effectiveness in the above shown stress resistance assays, further tests focused solely on treatments with E. ulmoides and C. chinensis extracts.
C. chinensis and E. ulmoides Enhanced Longevity
In addition to the survival under stressful conditions, the lifespan was also monitored under standard laboratory conditions. C. elegans exposed to E. ulmoides or C. chinensis extract featured a significant increase of the mean lifespan, as shown in the representative survival curves (Figure 3). C. chinensis caused the highest increase of the mean lifespan (24%), while E. ulmoides increased it by only 9%. Quercetin was used as a positive control and its increase in mean lifespan by 19% ranged between the effects from E. ulmoides and C. chinensis. Furthermore, all treatments were able to increase the minimum, median and maximum lifespan (Table 4).
FIGURE 3. Effects of C. chinensis and E. ulmoides extracts on the lifespan of wild type C. elegans.(A) Representative survival curves as well as (B) the mean lifespan ± SEM from three biological replicates are shown; 100 µM quercetin was used as a positive control. Significant differences were determined by a log-rank test and subsequent Bonferroni correction with *p < 0.05; **p < 0.01; ***p < 0.001 or ****p < 0.0001.
TABLE 4. Effect of C. chinensis and E. ulmoides on the lifespan of C. elegans.
C. chinensis Increased the Pharyngeal Pumping Frequency and Food Intake of C. elegans
C. elegans feeds by a cycle of contraction and relaxation of the pharyngeal muscles, which can be monitored by up and down movement of the grinder. The pharyngeal pumping rate in C. elegans determines the food intake and mirrors the general fitness status of the organism. The pumping was measured in wild-type adult animals on the 7th and 12th days of adulthood in the presence or absence of the plant extracts. The pumping frequencies of the pharynx of untreated nematodes (control) were about 128 and 42 pumps per minute on the 7th and 12th days of adulthood, respectively. C. chinensis exposure led to a significant increase of the pharyngeal pumping rate, with 150 and 53 pumps per minute on the 7th and 12th day of adulthood, respectively. On the other hand, the treatment with E. ulmoides did not lead to any frequency changes in either age class (Figure 4A).
FIGURE 4. Influences of E. ulmoides and C. chinensis extracts on (A) pharynx pumping rate and (B) intake of the E. coli strain OP50-GFP. Synchronized L4 larvae were transferred to NGM plates in the absence (control) or presence (30 μg/ml) of the respective plant extract. On the 7th and 12th days of adulthood, the pharyngeal pumping rate of individuals (n = 50) was measured under a stereoscopic microscope for 60 s. The results represent mean ± SEM and significant changes to the control are considered *(p < 0.05), **(p < 0.01), ***(p < 0.001) or ****(p < 0.0001) according to one-way ANOVA and post-hoc Bonferroni test.
In addition, food intake was evaluated by measuring the intensity of fluorescence in the intestinal lumen after feeding the worms with the E. coli strain OP50-GFP for 15 min. The analysis of fluorescent intensity revealed that the C. chinensis extract increased the amount of OP50-GFP bacteria in the worms’ intestine significantly by 34% on the 7th day and non-significantly by 12% on the 12th day of adulthood compared with the control group. The treatment with the E. ulmoides extract resulted in non-significant increase in the food uptake by only 9% on the 7th and 12th day of adulthood, respectively (Figure 4B).
C. chinensis Reduced the Accumulation of Autofluorescent Material
Autofluorescent material accumulates over time in cells and tissues with low turnover (Terman and Brunk, 2006), and is often used as a marker of aging in C. elegans (Pincus and Slack, 2010). We evaluated the intestinal autofluorescent material accumulation, as a marker of aging, of C. elegans on the 7th and 12th days of adulthood. C. elegans treated with C. chinensis showed significantly reduced autofluorescence intensities to 96.1 and 94.2% on the 7th and 12th days of adulthood, respectively, compared with the untreated group (100%). In contrast, treatment with E. ulmoides had no impact on red fluorescence in either age group (Figure5).
FIGURE 5. Red autofluorescence of wild-type C. elegans treated with 30 μg/ml plant extract. On the 7th and 12th days of adulthood, treated and untreated nematodes were photographed in bright field as well as under red fluorescent light. (A) Representative control and C. chinensis-treated nematode on the 12th day of adulthood (B) The fluorescence intensity of individuals was determined by densitometric analysis using the CellProfiler software. The results are the average intensity for two independent experiments (n ≥ 25 per experiment). The intensity was represented as mean ± SEM and the significant changes to the control are considered *(p < 0.05), **(p < 0.01), ***(p < 0.001), or ****(p < 0.0001) according to one-way ANOVA and post-hoc Bonferroni test.
C. chinensis Enhanced Locomotor Fitness in Liquid Media
We investigated the ability of C. chinensis and E. ulmoides to improve the nematode’s swimming behavior on the 7th and 12th days of adulthood by measuring four parameters: wave initiation rate, activity index, brush stroke, and body wave number. Indeed, the data obtained in the current study verify the age-dependence of all selected swim parameters when comparing the 7th and 12th days of adulthood (Figure 6). On the 7th day of adulthood, C. chinensis could increase the wave initiation rate to 56.6 waves per minute comparing with the control (47 waves per minute). Furthermore, the brush stroke was also enhanced by C. chinensis and at least a positive, albeit not statistically significant, trend is visible for the other two parameters. Interestingly, C. chinensis caused stronger effects on the 12th day of adulthood with statistically significant improvements in all measured parameters. By contrast, E. ulmoides did not produce any statistically significant difference on the swimming performance of C. elegans (Figure 6).
FIGURE 6. Swim performance of C. elegans after C. chinensis and E. ulmoides treatment. Wave initiation rate, body wave number, brush stroke and activity index were determined on the 7th and 12th day of adulthood. Error bars are the standard error of the mean (SEM) and one bar represents n ≥ 50 from two independent trials. Statistical significance was determined according to one-way ANOVA and post-hoc Bonferroni test as *(p < 0.05), **(p < 0.01), ***(p < 0.001), or ****(p < 0.0001).
Only Slight Impacts of E. ulmoides and C. chinensis on Body Size and Reproduction
In the current study, we measured the effect of E. ulmoides and C. chinensis on the growth as well as reproductive fitness of C. elegans. Indeed, after measuring the body area of treated and untreated worms on the 7th and 12th days of adulthood, the results showed that none of the tested TCM preparations had a significant effect on the body size of C. elegans, whereas a slight reducing trend is visible on the 12th day of adulthood in C. chinensis treated worms (Figure 7A). Moreover, exposure to E. ulmoides and C. chinensis did not significantly influence reproductive fitness in terms of the total number of progenies produced per individual (Figure 7B). However, we found that E. ulmoides and C. chinensis delayed the reproduction by decreasing the number of offspring on the first (E. ulmoides; not significant) or second (C. chinensis) day, respectively (Figure 7B). Interestingly, the extract treated worms led to a significant increase of the offspring on the third day of observation. Our results revealed that the selected TCM preparations increased the healthspan of C. elegans without significantly impairing overall growth, but with a slight impact on the timing of reproduction, which may reflect a delay in reproductive (ovarian) aging.
FIGURE 7. Impact of E. ulmoides and C. chinensis on body size and reproduction of C. elegans. (A) Body size of C. elegans on the 7th and 12th day of adulthood after TCM treatment (n ≥ 25 per experiment). (B) The number of offspring per day and in total during exposure to TCM treatment (n = 27–30 nematodes per treatment). The results display the average size for three independent experiments and error bars represent the SEM. Statistically significant differences to the control are considered with *p < 0.05 according to one-way ANOVA and post-hoc Bonferroni test.
C. chinensis Treatment Increased the Transcription of Heat Shock Protein Hsp-16.1
To get a first insight into the molecular mechanism of the observed healthspan effects, the gene expression for five heat shock proteins, namely daf-21 (hsp-90), hsp-16.1, hsp-16.2, hsp-70, and hsp-12.6, was analyzed in extract-treated nematodes on the 12th day of adulthood. The exposure to C. chinensis significantly upregulated the expression of hsp-16.1 by 3.8-fold (Figure 8). Moreover, the expression of hsp-12.6 also increased after C. chinensis treatment by 2.9-fold; however, due to the high variance and limited sample number, the significance threshold was not reached. Interestingly, treatment with E. ulmoides revealed no significant changes in the expression level of any tested genes.
FIGURE 8. Effect of C. chinensis and E. ulmoides treatment on the gene expressions levels of heat-shock proteins at the 12th day of adulthood. Gene expression was determined via qPCR, and expression values were calculated according to Pfaffl (2001). Gene expression values of the reference genes act-1 and cdc-42 were used to normalize the data. The graph shows the mean of three biological replicates. The error bars show the standard error of the mean (SEM) and statistical significance was determined according to one-way ANOVA and post-hoc Bonferroni test with *(p < 0.05).
Discussion
An organism’s lifespan is inevitably accompanied by the aging process, which involves functional decline, a steady increase of a plethora of chronic diseases, and ultimately death. Thus, it has been an ongoing dream of mankind to improve healthspan and extend life. In the last century, developed countries have profited from medical advances, improvements in public healthcare systems, and better living conditions derived from their socioeconomic power to achieve a remarkable increase in life expectancy. However, according to the WHO, age itself remains the greatest risk factor for all major life-threatening disorders, and the number of people suffering from age-related diseases is anticipated to almost double over the next two decades. The fact that healthspan has not increased at the same pace as lifespan is a source of grave concern (de Cabo et al., 2014). Attempts for living longer and healthier have attracted the attention of both the public and researchers. One interesting method for inducing a longer and healthier life is dietary supplementation with TCM. This study evaluated the anti-aging properties and health-related impacts of C. chinensis and E. ulmoides using C. elegans. Indeed, various nutritional and pharmacological interventions with C. chinensis and E. ulmoides have been previously shown. For a detailed overview of the bioactivities reported for C. chinensis and E. ulmoides see Tables 5, 6.
TABLE 5. Pharmacological effects and bioactivities of Eucommia ulmoides Oliver.
TABLE 6. Pharmacological effects and bioactivities of Cuscuta chinensis Lam.
Hormesis: A Possible Explanation for Age-dependent Effects of TCM-Mediated Heat Stress Resistance
In this study, the tested TCMs showed positive impact on heat stress resistance of C. elegans exposed to a heat shock (37 °C for 3 h) at different ages of adulthood (3rd, 7th, and 12th days). The beneficial effect of TCMs on heat stress resistance is consistent with other studies in C. elegans that showed enhancement of both, thermal tolerance and lifespan by other TCM-derived extracts, such as extracts from Zanthoxylum armatum DC. and Salvia miltiorrhiza Bunge (Pandey et al., 2019; Zhang et al., 2019). In addition, previous studies reported that quercetin, a common flavonoid found in TCMs, increased the tolerance to thermal stress, which was applied to the worms by heating them to 37 °C (Liu et al., 2016) or 35 °C (Saul et al., 2008). Thus, the enhancement of heat stress resistance by TCM could be based on a bioactive compound common to many of these medicinal plants that may regulate stress-signaling pathways. Alternatively, these compounds may act themselves as chemical chaperones that stabilize protein conformation and promote a general cellular stress response as described by Benedetti et al. (2008).
We could show that the ability of the TCMs to enhance stress resistance depends on the age of nematodes during test-performance. But what do the different tested adult-stages stand for? The 3rd day of adulthood reflects the time when the nematode is reproductive, very active and healthy; thus, symptoms of aging are not readily apparent. However, Kauffman et al. (2010) could show that learning and memory abilities are surprisingly impaired already at this young stage. On the 7th day of adulthood, reproduction came to a halt, movements are slower, stress resistance is decreased and long-term memory vanishes (Kauffman et al., 2010); thus, the effects of aging become visible. On the 12th day of adulthood, aging strongly emerges with the appearance of immobile nematodes, high stress-sensitivity, morphological abnormalities and deaths. Since the general anti-aging properties of TCMs are the main focus of this paper, results from the 7th and especially 12th day of adulthood were deemed more relevant.
The question arises why so many extracts are active in young, but not in old nematodes? The answer could be related to the hormesis effect, which is the biphasic response to low and high doses of numerous chemical, biological, or physical insults (Kendig et al., 2010). Several studies suggested the hormesis effect as the main mechanism underlying the beneficial effects of natural extracts and polyphenols (Saul et al., 2011; Martel et al., 2019), which is sometimes also called more specifically “xenohormesis” (Suter and Lucock, 2017). It is known that hormesis is dependent on the timing and duration of treatment (Tyne et al., 2015) and that the adaptive response to (mild) stressors is less active in aged individuals (Pomatto and Davies, 2017). Therefore, the lack of activity for most of the tested extracts in old nematodes could be due to their hormetic action being less efficient in old nematodes and/or in long-term treatment scenarios. For the few plant preparations that show positive effects in older nematodes, a mechanism distinct from hormesis may be at work.
To test whether hormesis might also explain the beneficial effects of C. chinensis and E. ulmoides in old nematodes, the gene expression of five hsp-genes was determined in treated and untreated animals on the 12th day of adulthood. Molecular chaperones were shown to be implicated in adaptive stress response and hormesis (Lakshmi et al., 2020), and especially heat shock proteins and their chaperone-activities play a fundamental role in hormesis in C. elegans (Zhao and Wang, 2012). Therefore, daf-21 (hsp-90) was specifically selected due to its implication in pathogenic stress resistance (JebaMercy et al., 2016; Balasubramaniam et al., 2019), in the stabilization of the ageing-relevant nutrient-sensor SIR-2.1 (Nguyen et al., 2018) and the DAF-16-mediated lifespan regulation (Somogyvári et al., 2018). Furthermore, in response to hormetic life- and healthspan prolonging treatments, the up-regulation of hsp-16.1 (Yanase et al., 1999; Lin et al., 2019), hsp-16.2 (Olsen et al., 2006; Hartwig et al., 2009), as well as hsp-70 and hsp-12.6 (Pietsch et al., 2011) was described. In our study, only hsp-16.1 was differentially expressed and a clear, but not statistically significant, trend was observed for hsp-12.6. Both genes were up-regulated after chronic C. chinensis exposure, whereas no response was detected after E. ulmoides treatment. This underlines the different mode of action of these two extracts, and suggests a possible hormetic action of the C. chinensis extract.
Increased Pathogen Resistance due to a TCM-Mediated Boost of the Immune Response?
P. luminescens has been reported as a nematode-symbiotic bacterium that causes a lethal intestinal infection in C. elegans and consequently reduces the worm’s developmental, survival and reproductive capacity (Sato et al., 2014; Hoinville and Wollenberg, 2018). In this study, the ability was investigated of TCM extracts to counteract the pathogen-induced mortality in C. elegans exposed to P. luminescens. E. ulmoides and C. chinensis increased the pathogenic resistance of C. elegans against P. luminescens by extending the survival during pathogen exposure in young and middle-aged nematodes. Interestingly, E. ulmoides and C. chinensis did not show direct antibacterial effects on P. luminescens or E. coli; thus, the antimicrobial impact of these TCMs did not play a role in the observed pathogenic resistance. Other published pathogen stress resistance trials in C. elegans reveal possible underlying mechanisms: resistance against Pseudomonas aeruginosa infections has been caused by many natural or synthetic compounds that target host immune responses to attenuate infections (Pukkila-Worley et al., 2012; Kong et al., 2016). The extract from Swietenia macrophylla seeds, for instance, promotes the survival of P. aeruginosa-challenged nematodes by boosting the expression of a C. elegans lysozyme encoding gene (lys-7) (Dharmalingam et al., 2012). Also, the natural polyphenols isolated from Magnolia plant species (honokiol and magnolol) promoted a cellular immune response and slowed down C. elegans killing by Staphylococcus aureus (Choi et al., 2015). Whether a boosted immune response is also responsible for the observed effects in the current study needs to be addressed in future molecular biological experiments.
Only C. chinensis Is an Overall Healthspan Enhancer
Besides stress resistance and lifespan, C. chinensis is also able to improve further ageing-related parameters, in fact, the pharyngeal pumping rate, locomotion and autofluorescence in older C. elegans.
The pharyngeal pumping rate in C. elegans determines the food intake and mirrors the general fitness status of the organism. C. chinensis exposure led to a significant increase of the pharyngeal pumping frequency of middle-aged and old C. elegans, whereas the treatment with E. ulmoides had no impact on the pumping rate. Several studies reported that an increased pharyngeal pumping is a sign of rejuvenation, since the pumping rate declines gradually with increasing age (Huang et al., 2004). Thus, the increased pumping frequency in C. chinensis-treated nematodes could be a sign of slower aging. This result is consistent with a recent report of Zeng et al. (2019). They investigated Jianpi-yangwei, a traditional Chinese medicine primarily used to treat functional decline related to aging in humans, and showed that it also slowed the decline in pharyngeal pumping during aging in C. elegans. In addition, polyphenols, which are suggested to be the main active TCM ingredient in our plants (Liu and Henkel, 2002), are able to increase the pumping rate as shown e.g. for blueberry polyphenols (Wilson et al., 2006). On the other hand, previous studies suggested that decreased pumping frequency is a cause of health- and lifespan enhancement in C. elegans, with dietary restriction as the underlying reason (Lakowski and Hekimi, 1998). However, this mode of action can be excluded for the two tested extracts since no reduction in the pumping rate or food intake was observed subsequent to the treatment.
Autofluorescent material (sometimes referred to as lipofuscin or “age pigment”) builds up over time in cells and tissues with low turnover (Terman and Brunk, 2006), and is often used as a marker of aging in C. elegans (Pincus and Slack, 2010). The transparent nature of C. elegans facilitates the examination of that autofluorescent material in living subjects (Gerstbrein et al., 2005). It was shown in C. elegans that the red autofluorescence, which is mainly located in the intestine, reflects the aging status of the individual in the most reliable way (Pincus et al., 2016). Our results revealed that C. chinensis-treated C. elegans showed significantly reduced autofluorescence intensities; however, the treatment with E. ulmoides had no impact on the accumulation of autofluorescent material. Indeed, several TCMs have been reported as important sources of active compounds that decrease the accumulation of autofluorescent material in C. elegans, but most are hard to compare to our work. For instance, a Panax notoginseng preparation astonishingly reduced the autofluorescence by roughly one half (Jin et al., 2019). However, the concentrations used were more than 25 times higher compared to the current study. Moreover, blue instead of red fluorescence was used, which is not suitable as an aging marker (Pincus et al., 2016). Also a Jianpi-yangwei preparation was used successfully to reduce the autofluorescence (Zeng et al., 2019), but again, the concentration was higher than in the current study, and green rather than red fluorescence was used. It remains unclear whether other TCM preparations at low concentrations exert similar activities, since the required studies have not been carried out.
Locomotion is one of the most important features that reflect the physical ability of nematodes and the aging stage (Li et al., 2019a). This study evaluated the ability of C. chinensis and E. ulmoides to upgrade the nematode’s swimming by measuring wave initiation rate, activity index, brush stroke, and body wave number. The wave initiation rate is the number of body waves per minute, which indicates the movement-speed, whereas the body wave number determines the waviness of the body at each time point. Furthermore, the activity index adds up the number of pixels that are covered by the nematode during the time spent for two strokes as an indicator for the vigorousness of bending over time. Finally, the brush stroke parameter reflects the area covered by the body in a complete stroke, which indicates the depth of the movement. Both latter parameters were normalized to the body size. These features were selected due to their strong age dependence, since the body wave number is increasing and the remaining parameters are decreasing with age (Restif et al., 2014). Interestingly, C. chinensis could significantly improve all measured locomotion parameters particularly in old C. elegans. On the other hand, E. ulmoides did not produce any statistically significant difference on the swimming performance of C. elegans. This is in line with the observations for the autofluorescence and the pharyngeal pumping, which together indicate that E. ulmoides is not a general anti-aging extract, but specifically acts on stress resistance. On the contrary, C. chinensis provoked beneficial effects in all life- and healthspan parameters and could be regarded as an overall anti-aging plant preparation.
To quantify the benefit of the extract treatments, the results were compared to quercetin. The polyphenol quercetin is a prominent and robust health- and lifespan enhancer which is studied usually in a range of 50–200 µM with overall beneficial effects in C. elegans. The survival after heat stress, starting between the 2nd and 6th day of adulthood, was increased by 8% (Zhou et al., 2018), 14% (Upadhyay et al., 2013; Büchter et al., 2015), or 20% (Saul et al., 2008), respectively, in quercetin-treated nematodes. By using comparable ages and test performances, C. chinensis increased the survival by 19–24% and E. ulmoides by 22–38%, thus, mostly exceeding the beneficial effects previously published for quercetin. Furthermore, the motility (Sugawara and Sakamoto, 2020) and pharyngeal pumping (Pietsch et al., 2011) were significantly enhanced in aged nematodes, and blue autofluorescence decreased by 28% in moderately aged nematodes (Büchter et al., 2015) after quercetin treatment. C. chinensis treatment resulted in similar effects, but could decrease the autofluorescence only by 4–6%. However, we measured red instead of blue autofluorescence, as suggested by Pincus et al. (2016). Thus, a direct comparison is not possible. Quercetin treatments also increase mean lifespan by 10% (Saul et al., 2008), 15% (Kampkötter et al., 2008), 18% (Pietsch et al., 2009), or 19% (Büchter et al., 2015), respectively. This is in line with our lifespan extension of 19% by 100 µM quercetin (which corresponds to about 30 μg/ml). Nematodes treated with 30 μg/ml C. chinensis surpass these values by more than 5%, whereas E. ulmoides only induced a slight life extension compared to quercetin. In total, C. chinensis effects seem to be comparable to quercetin, or are even somewhat stronger in terms of health- and lifespan prolongation.
Health-enhancements via TCMs only produce minor trade-off effects in reproductive traits. According to the life history theory there is a strong relationship between longevity, body size and fecundity (Fabian and Flatt, 2012). This theory suggests that an increase of body size can be balanced against the benefits of faster reproduction and the costs of lower offspring viability and lower initial fecundity, weighed against a backdrop of differential allocation of physiological and metabolic resources to each of these processes and to growth itself. (Woodruff et al., 2019). The change in both, biotic (predation pressure, resource abundance, pollinator density, etc.) and abiotic (temperature, salinity, humidity, etc.) environmental factors can have an impact on the survival, growth and fecundity of an organism (Woodruff et al., 2019). Thus, by increasing the survival through the usage of the TCM extracts, a trade-off in other life-history traits as described by Stearns (1989) and Zera and Harshman (2001) is conceivable. Therefore, we tried to exclude any side effects of E. ulmoides and C. chinensis on the growth as well as reproductive fitness of C. elegans. Our results revealed that E. ulmoides and C. chinensis increased the healthspan and lifespan of C. elegans without significantly impairing overall growth and offspring, but with a slight impact on the timing of reproduction. Some health-prolonging substances have already been found to delay or decrease reproductive output (Saul et al., 2013; Kim et al., 2014; Zhuang et al., 2014). For instance, Saul et al. (2011) and Saul et al. (2013) reported that after life- and healthspan promoting polyphenol exposures, the beneficial effects in C. elegans were associated with significant postponement of the initial reproduction. Moreover, other studies concluded that the lifespan and healthspan extensions caused by different substance exposures were often associated with reductions in growth of C. elegans (Liao et al., 2011; Saul et al., 2013). Curcumin, as an active ingredient in the herbal medicine turmeric (Curcuma longa), caused lifespan and healthspan extension in C. elegans in parallel to body size decrease (Liao et al., 2011). Furthermore, the two polyphenolic tannin components tannic acid and catechin were able to extend the life- and healthspan of C. elegans, but with significant reduction in the body size (Saul et al., 2013). On the other hand, several studies found healthspan prolongation as well as improved fecundity in parallel (Ryu et al., 2016; Zhang et al., 2018b). In many cases, however, enhanced survival is not linked to a trade-off in reproduction or growth traits. Scerbak and colleagues found that lifespan-extending Blueberry and fungus treatments did not influence total progeny produced per individual over reproductive lifespan (Scerbak et al., 2016). Tyrosol, a main phenol in extra virgin olive oil, increased healthspan and did not affect other fitness parameters in C. elegans, such as growth and reproduction. Although treated nematodes showed a slight decrease in body size at the L4 larval stage, the body size on the first and sixth adult day was not affected (Cañuelo et al., 2012).
General Discussion and Limitations of Our Study
All the selected TCM preparations are claimed to have anti-aging activity, and most indeed show effects on some of the aging parameters that we measured in C. elegans. However, deeper phenotyping reveals differences in their effects. In several cases, effects are only observed at earlier ages (corresponding to adult animals). Even for the two extracts that show activity in older worms, the spectrum of effects is different. A decrease in resistance to various stresses is generally viewed as a hallmark of aging, but reversing this effect apparently does not suffice to combat also other signs of aging, like locomotor ones. This is consistent with the observation that different physiological systems (locomotor, cardiovascular, immune, etc.) may age at divergent rates in different individuals. Nonetheless, some molecular processes probably underlie aging in general, and would therefore affect all organs and tissues. Plant extracts with widespread anti-aging effects across physiological systems therefore likely affect such generic molecular aging processes, and are most attractive therapeutically.
Our study has a number of limitations. Effects in C. elegans do not necessarily extrapolate to other animals, even though most aging mechanisms and pathways in C. elegans have counterparts in mammals. Moreover, anti-aging effects of C. chinensis have been documented in animals, and are attested in TCM for humans (Donnapee et al., 2014). In any case, mechanistic studies will be easier in C. elegans. Although the precise anti-aging mechanism of the TCM extracts remains to be elucidated, differences between them have already become apparent. We only tested a single dose of the TCM plant extracts, and only a single solvent was used for extraction. It is conceivable that preparations at higher doses or prepared with other solvents could be more efficacious, but they may also prove to be more toxic. It is not clear whether the anti-aging compounds from the plant extracts are absorbed via the intestine after ingestion, or penetrate the C. elegans cuticle. However, in mice as well as rats, oral administration of C. chinensis preparations showed anti-aging effects. The plant extracts were administered to C. elegans from the beginning of the L4 stage; it is therefore not clear whether they would be equally effective if started at later times in life. Several of the phytochemicals documented in C. chinensis have anti-aging potential, but their relative contributions and potential synergies would be quite hard to study in mammals; however, this should be feasible in C. elegans.
Conclusion
This study demonstrates the different effects (and possible modes of action) of the tested TCM extracts. E. ulmoides affected specific physiological characteristics by improving the survival probability of C. elegans during and after stress. On the other hand, C. chinensis enhanced the healthspan in a more general way by improving the physical fitness, the stress resistance and the “autofluorescence” aging biomarker. In addition, C. chinensis exposure led to a stronger life-prolonging effect under standard laboratory conditions compared to E. ulmoides, and the upregulation of hsp-16.1 was only observed after C. chinensis treatment. Furthermore, extracts from L. lucidum, P. cocos, A. membranaceus, S. chinensis, and A. bidentata were beneficial for C. elegans in stress conditions at younger age. The analysis of additional endpoints and/or concentrations could further uncover their potential and their relation to a hormesis effect. Collectively, our results suggest that TCM extracts have a promising capability to modulate healthspan in a beneficial way, with C. chinensis having the greatest potential to be an effective antiaging treatment out of the seven extracts tested.
Data Availability Statement
The original contributions presented in the study are included in the article/Supplementary Material, further inquiries can be directed to the corresponding author.
Author Contributions
Conceptualization, SS, CS-L, WL, and NS; methodology, SS and NS; validation, NS and CS-L; formal analysis, SS; investigation, SS; resources, KS; data curation, SS and NS; writing—original draft preparation, SS; writing—review and editing, CS-L, WL, KS, and NS; visualization, SS; supervision, NS and CS-L; project administration, WL, CS-L, and NS; funding acquisition, WL, CS-L, and NS All authors have read and agreed to the published version of the manuscript.
Funding
This project has received funding from the European Union’s Horizon 2020 research and innovation program under Grant agreement No 633589 (Aging with Elegans). This publication reflects only the authors’ views and the Commission is not responsible for any use that may be made of the information it contains. In addition, a financial support from the Yousef Jameel Academic Program at the Humboldt-University of Berlin, Germany is gratefully acknowledged. Finally, we acknowledge support by the German Research Foundation (DFG) and the Open Access Publication Fund of Humboldt-Universität zu Berlin.
Conflict of Interest
Author KS was employed by the company AnalytiCon Discovery GmbH, Germany.
The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Acknowledgments
We thank the Caenorhabditis Genetics Center, which is funded by the National Institutes of Health National Center for Research Resources, for the supply of the Caenorhabditis elegans strains, and Prof. Qingfei Liu (Tsinghua University, Beijing, China) for help with the selection and acquisition of the TCM plant material. Not least, we thank Thea Böttcher and Shumon Chakrabarti for their technical support in the lab, and Christian E. W. Steinberg for enabling this project.
Supplementary Material
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fphar.2021.604435/full#supplementary-material.
References
Balasubramaniam, B., Vinitha, T., Deepika, S., Jebamercy, G., Venkatakrishna, L. M., and Balamurugan, K. (2019). Analysis of Caenorhabditis elegans phosphoproteome reveals the involvement of a molecular chaperone, HSP-90 protein during Salmonella enterica Serovar Typhi infection. Int. J. Biol. Macromol. 137, 620–646. doi:10.1016/j.ijbiomac.2019.06.085
Benedetti, M. G., Foster, A. L., Vantipalli, M. C., White, M. P., Sampayo, J. N., Gill, M. S., et al. (2008). Compounds that confer thermal stress resistance and extended lifespan. Exp. Gerontol. 43, 882–891. doi:10.1016/j.exger.2008.08.049
Break-with-Traditon (2019). The World Health Organization’s decision to include traditional Chinese medicine in its global diagnostic compendium could backfire. Nature 570, 5. doi:10.1038/d41586-019-01726-1
Brenner, S. (1974). The genetics of Caenorhabditis elegans. Genetics 77, 71–94. doi:10.1093/genetics/77.1.71
Büchter, C., Zhao, L., Havermann, S., Honnen, S., Fritz, G., Proksch, P., et al. (2015). TSG (2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside) from the Chinese herb polygonum multiflorum increases life span and stress resistance of Caenorhabditis elegans. Oxid. Med. Cell Longev. 2015, 124357. doi:10.1155/2015/124357
Cañuelo, A., Gilbert-López, B., Pacheco-Liñán, P., Martínez-Lara, E., Siles, E., and Miranda-Vizuete, A. (2012). Tyrosol, a main phenol present in extra virgin olive oil, increases lifespan and stress resistance in Caenorhabditis elegans. Mech. Ageing Dev. 133, 563–574. doi:10.1016/j.mad.2012.07.004
Choi, E. J., Kim, H. I., Kim, J. A., Jun, S. Y., Kang, S. H., Park, D. J., et al. (2015). The herbal-derived honokiol and magnolol enhances immune response to infection with methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant S. aureus (MRSA). Appl. Microbiol. Biotechnol. 99, 4387–4396. doi:10.1007/s00253-015-6382-y
Cui, H., Dong, P., and Chen, B. (2019). Effect of total flavonoids of Cuscuta chinensis Lam. (Convolvulaceae) on oxidative stress injury in mouse testis and epididymis, and on serum levels of reproductive hormones in oligoasthenospermia mice model. Trop. J. Pharmaceut. Res. 18, 1253–1258. doi:10.4314/tjpr.v18i6.15
De cabo, R., Carmona-Gutierrez, D., Bernier, M., Hall, M. N., and Madeo, F. (2014). The search for ntiaging interventions: from elixirs to fasting regimens. Cell 157, 1515–1526. doi:10.1016/j.cell.2014.05.031
Dharmalingam, K., Tan, B. K., Mahmud, M. Z., Sedek, S. A., Majid, M. I., Kuah, M. K., et al. (2012). Swietenia macrophylla extract promotes the ability of Caenorhabditis elegans to survive Pseudomonas aeruginosa infection. J. Ethnopharmacol. 139, 657–663. doi:10.1016/j.jep.2011.12.016
Ding, H., and Wang, Z. (2015). Experience of treatment and understanding of impediment disease in Shen nong ben cao jing (Shen-ntonz’s Classic of Materia Medica). Zhonghua yi shi za zhi, 45, 3–6.
Ding, J. X., Li, W. L., Hu, Y., Song, H., Sun, X. M., and Ji, Y. B. (2019). Characterization of estrogenic active ingredients in Cuscuta chinensis Lam. based on spectral characteristics and high performance liquid chromatography/quadrupole time of flight mass spectrometry. Mol. Med. Rep. 19 (2), 1238–1247. doi:10.3892/mmr.2018.9755
Donnapee, S., Li, J., Yang, X., Ge, A. H., Donkor, P. O., Gao, X. M., et al. (2014). Cuscuta chinensis Lam.: a systematic review on ethnopharmacology, phytochemistry and pharmacology of an important traditional herbal medicine. J. Ethnopharmacol. 157, 292–308. doi:10.1016/j.jep.2014.09.032
Fuellen, G., Jansen, L., Cohen, A. A., Luyten, W., Gogol, M., Simm, A., et al. (2019). Health and aging: unifying concepts, scores, biomarkers and pathways. Aging Dis. 10, 883–900. doi:10.14336/AD.2018.1030
Fung, F. Y., and Linn, Y. C. (2015). Developing traditional Chinese medicine in the era of evidence-based medicine: current evidences and challenges. Evid. Based Complement Alternat. Med. 2015, 425037–425039. doi:10.1155/2015/425037
Gao, J. M., Li, R., Zhang, L., Jia, L. L., Ying, X. X., Dou, D. Q., et al. (2013). Cuscuta chinensis seeds water extraction protecting murine osteoblastic MC3T3-E1 cells against tertiary butyl hydroperoxide induced injury. J. Ethnopharmacol. 148, 587–595. doi:10.1016/j.jep.2013.05.005
Gerstbrein, B., Stamatas, G., Kollias, N., and Driscoll, M. (2005). In vivo spectrofluorimetry reveals endogenous biomarkers that report healthspan and dietary restriction in Caenorhabditis elegans. Aging Cell 4, 127–137. doi:10.1111/j.1474-9726.2005.00153.x
Ghazanfari, T., Naseri, M., Shams, J., and Rahmati, B. (2013). Cytotoxic effects ofCuscutaextract on human cancer cell lines. Food Agric. Immunol. 24, 87–94. doi:10.1080/09540105.2011.648608
Gruber, J., Chen, C. B., Fong, S., Ng, L. F., Teo, E., and Halliwell, B. (2014). Caenorhabditis elegans: what we can and cannot learn from aging worms. Antioxidants Redox Signal. 23, 256–279. doi:10.1089/ars.2014.6210
Gu, J., Wang, J. J., Yan, J., Cui, C. F., Wu, W. H., Li, L., et al. (2011). Effects of lignans extracted from Eucommia ulmoides and aldose reductase inhibitor epalrestat on hypertensive vascular remodeling. J. Ethnopharmacol. 133, 6–13. doi:10.1016/j.jep.2010.08.055
Han, S. K., Lee, D., Lee, H., Kim, D., Son, H. G., Yang, J. S., et al. (2016). OASIS 2: online application for survival analysis 2 with features for the analysis of maximal lifespan and healthspan in aging research. Oncotarget 7, 56147–56152. doi:10.18632/oncotarget.11269
Hao, S., Xiao, Y., Lin, Y., Mo, Z., Chen, Y., Peng, X., et al. (2016). Chlorogenic acid-enriched extract from Eucommia ulmoides leaves inhibits hepatic lipid accumulation through regulation of cholesterol metabolism in HepG2 cells. Pharm. Biol. 54, 251–259. doi:10.3109/13880209.2015.1029054
Hartwig, K., Heidler, T., Moch, J., Daniel, H., and Wenzel, U. (2009). Feeding a ROS-generator to Caenorhabditis elegans leads to increased expression of small heat shock protein HSP-16.2 and hormesis. Genes Nutr. 4, 59–67. doi:10.1007/s12263-009-0113-x
Hastings, J., Mains, A., Virk, B., Rodriguez, N., Murdoch, S., Pearce, J., et al. (2019). Multi-omics and genome-scale modeling reveal a metabolic shift during C. elegans aging. Front. Mol. Biosci. 6, 2. doi:10.3389/fmolb.2019.00002
Hirata, T., Kobayashi, T., Wada, A., Ueda, T., Fujikawa, T., Miyashita, H., et al. (2011). Anti-obesity compounds in green leaves of Eucommia ulmoides. Bioorg. Med. Chem. Lett. 21, 1786–1791. doi:10.1016/j.bmcl.2011.01.060
Hoinville, M. E., and Wollenberg, A. C. (2018). Changes in Caenorhabditis elegans gene expression following exposure to Photorhabdus luminescens strain TT01. Dev. Comp. Immunol. 82, 165–176. doi:10.1016/j.dci.2017.09.005
Horii, Y., Tanida, M., Shen, J., Hirata, T., Kawamura, N., Wada, A., et al. (2010). Effects of Eucommia leaf extracts on autonomic nerves, body temperature, lipolysis, food intake, and body weight. Neurosci. Lett. 479, 181–186. doi:10.1016/j.neulet.2010.05.030
Hosono, R. (1978). Sterilization and growth inhibition of Caenorhabditis elegans by 5-fluorodeoxyuridine. Exp. Gerontol. 13, 369–374. doi:10.1016/0531-5565(78)90047-5
Huang, C., Xiong, C., and Kornfeld, K. (2004). Measurements of age-related changes of physiological processes that predict lifespan of Caenorhabditis elegans. Proc. Natl. Acad. Sci. U.S.A. 101, 8084. doi:10.1073/pnas.0400848101
Jafarian, A., Ghannadi, A., and Mohebi, B. (2014). Cytotoxic effects of chloroform and hydroalcoholic extracts of aerial parts of Cuscuta chinensis and Cuscuta epithymum on Hela, HT29 and MDA-MB-468 tumor cells. Res. Pharm. Sci. 9, 115–122.
Jebamercy, G., Durai, S., Prithika, U., Marudhupandiyan, S., Dasauni, P., Kundu, S., et al. (2016). Role of DAF-21protein in Caenorhabditis elegans immunity against Proteus mirabilis infection. J. Proteomics 145, 81–90. doi:10.1016/j.jprot.2016.03.047
Jin, S.-Y., Li, D.-Q., Lu, S., Han, L.-T., Liu, D.-H., Huang, Z., et al. (2019). Ethanol extracts of Panax notoginseng increase lifespan and protect against oxidative stress in Caenorhabditis elegans via the insulin/IGF-1 signaling pathway. J. Funct. Foods 58, 218–226. doi:10.1016/j.jff.2019.04.031
Jin, X., Amitani, K., Zamami, Y., Takatori, S., Hobara, N., Kawamura, N., et al. (2010). Ameliorative effect of Eucommia ulmoides Oliv. leaves extract (ELE) on insulin resistance and abnormal perivascular innervation in fructose-drinking rats. J. Ethnopharmacol. 128, 672–678. doi:10.1016/j.jep.2010.02.019
Kaeberlein, M. (2018). How healthy is the healthspan concept? GeroScience 40, 361–364. doi:10.1007/s11357-018-0036-9
Kampkötter, A., Timpel, C., Zurawski, R. F., Ruhl, S., Chovolou, Y., Proksch, P., et al. (2008). Increase of stress resistance and lifespan of Caenorhabditis elegans by quercetin. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 149, 314–323. doi:10.1016/j.cbpb.2007.10.004
Kang, S. Y., Jung, H. W., Lee, M. Y., Lee, H. W., Chae, S. W., and Park, Y. K. (2014). Effect of the semen extract of Cuscuta chinensis on inflammatory responses in LPS-stimulated BV-2 microglia. Chin. J. Nat. Med. 12, 573–581. doi:10.1016/S1875-5364(14)60088-1
Kauffman, A. L., Ashraf, J. M., Corces-Zimmerman, M. R., Landis, J. N., and Murphy, C. T. (2010). Insulin signaling and dietary restriction differentially influence the decline of learning and memory with age. PLoS Biol. 8, e1000372. doi:10.1371/journal.pbio.1000372
Kendig, E. L., Le, H. H., and Belcher, S. M. (2010). Defining hormesis: evaluation of a complex concentration response phenomenon. Int. J. Toxicol. 29, 235–246. doi:10.1177/1091581810363012
Kim, B. H., Park, K. S., and Chang, I. M. (2009). Elucidation of anti-inflammatory potencies of Eucommia ulmoides bark and Plantago asiatica seeds. J. Med. Food 12, 764–769. doi:10.1089/jmf.2008.1239
Kim, J. S., Koppula, S., Yum, M. J., Shin, G. M., Chae, Y. J., Hong, S. M., et al. (2017). Anti-fibrotic effects of Cuscuta chinensis with in vitro hepatic stellate cells and a thioacetamide-induced experimental rat model. Pharm. Biol. 55, 1909–1919. doi:10.1080/13880209.2017.1340965
Kim, Y. S., Seo, H. W., Lee, M. H., Kim, D. K., Jeon, H., and Cha, D. S. (2014). Protocatechuic acid extends lifespan and increases stress resistance in Caenorhabditis elegans. Arch Pharm. Res. (Seoul) 37, 245–252. doi:10.1007/s12272-013-0183-6
Kirkland, J. L., and Peterson, C. (2009). Healthspan, translation, and new outcomes for animal studies of aging. J. Gerontol. A Biol. Sci. Med. Sci. 64, 209–212. doi:10.1093/gerona/gln063
Kobayashi, Y., Miyazawa, M., and Kojima, T. (2010). The use of Morus alba L. (Mulberry) and Eucommia ulmoides (Tochu) leaves as functional foods: a promising approach in the management of hyperlipidemia. J. Tradit. Med. 27, 225–230. doi:10.11339/jtm.27.225
Kocsisova, Z., Kornfeld, K., and Schedl, T. (2019). Rapid population-wide declines in stem cell number and activity during reproductive aging in C. elegans. Development 146, dev173195. doi:10.1242/dev.173195
Kong, C., Eng, S. A., Lim, M. P., and Nathan, S. (2016). Beyond traditional antimicrobials: a Caenorhabditis elegans model for discovery of novel anti-infectives. Front. Microbiol. 7, 1956. doi:10.3389/fmicb.2016.01956
Kunugi, H., and Mohammed Ali, A. (2019). Royal jelly and its components promote healthy aging and longevity: from animal models to humans. Int. J. Mol. Sci. 20, 4662. doi:10.3390/ijms20194662
Kwan, C. Y., Chen, C. X., Deyama, T., and Nishibe, S. (2003). Endothelium-dependent vasorelaxant effects of the aqueous extracts of the Eucommia ulmoides Oliv. leaf and bark: implications on their antihypertensive action. Vasc. Pharmacol. 40, 229–235. doi:10.1016/j.vph.2003.09.001
Kwon, S. H., Kim, M. J., Ma, S. X., You, I. J., Hwang, J. Y., Oh, J. H., et al. (2012). Eucommia ulmoides Oliv. Bark. protects against hydrogen peroxide-induced neuronal cell death in SH-SY5Y cells. J. Ethnopharmacol. 142, 337–345. doi:10.1016/j.jep.2012.04.010
Kwon, S. H., Lee, H. K., Kim, J. A., Hong, S. I., Kim, S. Y., Jo, T. H., et al. (2011). Neuroprotective effects of Eucommia ulmoides Oliv. Bark on amyloid beta (25–35)-induced learning and memory impairments in mice. Neurosci. Lett. 487, 123–127. doi:10.1016/j.neulet.2010.10.042
Kwon, S. H., Ma, S. X., Hwang, J. Y., Ko, Y. H., Seo, J. Y., Lee, B. R., et al. (2016). The anti-inflammatory activity of Eucommia ulmoides Oliv. Bark. Involves NF-κB suppression and Nrf2-dependent HO-1 induction in BV-2 microglial cells. Biomol Ther (Seoul) 24, 268–282. doi:10.4062/biomolther.2015.150
Kwon, S. H., Ma, S. X., Joo, H. J., Lee, S. Y., and Jang, C. G. (2013). Inhibitory effects of Eucommia ulmoides Oliv. Bark on scopolamine-induced learning and memory deficits in mice. Biomol Ther (Seoul) 21, 462–469. doi:10.4062/biomolther.2013.074
Lakowski, B., and Hekimi, S. (1998). The genetics of caloric restriction in Caenorhabditis elegans. Proc. Natl. Acad. Sci. U.S.A. 95, 13091–13096. doi:10.1073/pnas.95.22.13091
Lakshmi, P. K., Kumar, S., Pawar, S., Kuriakose, B. B., Sudheesh, M. S., and Pawar, R. S. (2020). Targeting metabolic syndrome with phytochemicals: focus on the role of molecular chaperones and hormesis in drug discovery. Pharmacol. Res. 159, 104925. doi:10.1016/j.phrs.2020.104925
Lam, W. C., Lyu, A., and Bian, Z. (2019). ICD-11: impact on traditional Chinese medicine and world healthcare systems. Pharmaceut. Med. 33, 373–377. doi:10.1007/s40290-019-00295-y
Lee, G.-H., Lee, H.-Y., Park, S.-A., Shin, T.-S., and Chae, H.-J. (2019). Eucommia ulmoides leaf extract ameliorates steatosis induced by high-fat diet in rats by increasing lysosomal function. Nutrients 11, 426. doi:10.3390/nu11020426
Lee, G. H., Lee, M. R., Lee, H. Y., Kim, S. H., Kim, H. K., Kim, H. R., et al. (2014). Eucommia ulmoides cortex, geniposide and aucubin regulate lipotoxicity through the inhibition of lysosomal BAX. PloS One 9, e88017. doi:10.1371/journal.pone.0088017
Li, C. S., Deng, H. B., Li, D. D., and Li, Z. H. (2013). Advances and challenges in screening traditional Chinese anti-aging materia medica. Chin. J. Integr. Med. 19, 243–252. doi:10.1007/s11655-013-1432-8
Li, G., Gong, J., Liu, J., Liu, J., Li, H., Hsu, A.-L., et al. (2019a). Genetic and pharmacological interventions in the aging motor nervous system slow motor aging and extend life span in C. elegans. Science Advances 5, eaau5041. doi:10.1126/sciadv.aau5041
Li, Y., Xie, Y., He, Y., Hou, W., Liao, M., and Liu, C. (2019b). Quality markers of traditional Chinese medicine: concept, progress, and perspective. Engineering 5, 888–894. doi:10.1016/j.eng.2019.01.015
Liao, J. C., Chang, W. T., Lee, M. S., Chiu, Y. J., Chao, W. K., Lin, Y. C., et al. (2014). Antinociceptive and anti-Inflammatory activities of Cuscuta chinensis seeds in mice. Am. J. Chin. Med. 42, 223–242. doi:10.1142/S0192415X14500153
Liao, V. H., Yu, C. W., Chu, Y. J., Li, W. H., Hsieh, Y. C., and Wang, T. T. (2011). Curcumin-mediated lifespan extension in Caenorhabditis elegans. Mech. Ageing Dev. 132, 480–487. doi:10.1016/j.mad.2011.07.008
Lin, C., Zhang, X., Xiao, J., Zhong, Q., Kuang, Y., Cao, Y., et al. (2019). Effects on longevity extension and mechanism of action of carnosic acid in Caenorhabditis elegans. Food Funct. 10, 1398–1410. doi:10.1039/c8fo02371a
Liu, H., Li, K., Zhao, J., and Deng, W. (2018). Effects of polyphenolic extract from Eucommia ulmoides Oliver leaf on growth performance, digestibility, rumen fermentation and antioxidant status of fattening lambs. Anim. Sci. J. 89, 888–894. doi:10.1111/asj.12998
Liu, J., and Henkel, T. (2002). Traditional Chinese medicine (TCM): are polyphenols and saponins the key ingredients triggering biological activities? Curr. Med. Chem. 9, 1483–1485. doi:10.2174/0929867023369709
Liu, Z., Zhao, J., Li, W., Shen, L., Huang, S., Tang, J., et al. (2016). Computational screen and experimental validation of anti-influenza effects of quercetin and chlorogenic acid from traditional Chinese medicine. Sci. Rep. 6, 19095. doi:10.1038/srep19095
Luo, L. F., Wu, W. H., Zhou, Y. J., Yan, J., Yang, G. P., and Ouyang, D. S. (2010). Antihypertensive effect of Eucommia ulmoides Oliv. extracts in spontaneously hypertensive rats. J. Ethnopharmacol. 129, 238–243. doi:10.1016/j.jep.2010.03.019
Luyten, W., Antal, P., Braeckman, B. P., Bundy, J., Cirulli, F., Fang-Yen, C., et al. (2016). Ageing with elegans: a research proposal to map healthspan pathways. Biogerontology 17, 771–782. doi:10.1007/s10522-016-9644-x
Martel, J., Ojcius, D. M., Ko, Y.-F., Ke, P.-Y., Wu, C.-Y., Peng, H.-H., et al. (2019). Hormetic effects of phytochemicals on health and longevity. Trends in Endocrinology & Metabolism 30, 335–346. doi:10.1016/j.tem.2019.04.001
Mcquin, C., Goodman, A., Chernyshev, V., Kamentsky, L., Cimini, B. A., Karhohs, K. W., et al. (2018). CellProfiler 3.0: next-generation image processing for biology. PLoS Biol. 16, e2005970. doi:10.1371/journal.pbio.2005970
Nguyen, M. T., Somogyvári, M., and Sőti, C. (2018). Hsp90 stabilizes SIRT1 orthologs in mammalian cells and C. elegans. Int. J. Mol. Sci. 19. doi:10.3390/ijms19113661
Olsen, A., Vantipalli, M. C., and Lithgow, G. J. (2006). Lifespan extension of Caenorhabditis elegans following repeated mild hormetic heat treatments. Biogerontology 7, 221–230. doi:10.1007/s10522-006-9018-x
Pandey, T., Sammi, S. R., Nooreen, Z., Mishra, A., Ahmad, A., Bhatta, R. S., et al. (2019). Anti-ageing and anti-Parkinsonian effects of natural flavonol, tambulin from Zanthoxyllum aramatum promotes longevity in Caenorhabditis elegans. Exp. Gerontol. 120, 50–61. doi:10.1016/j.exger.2019.02.016
Peñuelas-Urquides, K., Villarreal-Treviño, L., Silva-Ramírez, B., Rivadeneyra-Espinoza, L., Said-Fernández, S., and León, M. B. d. (2013). Measuring of Mycobacterium tuberculosis growth: a correlation of the optical measurements with colony forming units. Braz. J. Microbiol. 44, 287–290. doi:10.1590/s1517-83822013000100042
Pfaffl, M. W. (2001). A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 29, e45. doi:10.1093/nar/29.9.e45
Pietsch, K., Saul, N., Chakrabarti, S., Stürzenbaum, S. R., Menzel, R., and Steinberg, C. E. (2011). Hormetins, antioxidants and prooxidants: defining quercetin-, caffeic acid- and rosmarinic acid-mediated life extension in C. elegans. Biogerontology 12, 329–347. doi:10.1007/s10522-011-9334-7
Pietsch, K., Saul, N., Menzel, R., Stürzenbaum, S. R., and Steinberg, C. E. (2009). Quercetin mediated lifespan extension in Caenorhabditis elegans is modulated by age-1, daf-2, sek-1 and unc-43. Biogerontology 10, 565–578. doi:10.1007/s10522-008-9199-6
Pincus, Z., Mazer, T. C., and Slack, F. J. (2016). Autofluorescence as a measure of senescence in C. elegans: look to red, not blue or green. Aging (Albany NY) 8, 889–898. doi:10.18632/aging.100936
Pincus, Z., and Slack, F. J. (2010). Developmental biomarkers of aging in Caenorhabditis elegans. Dev. Dynam. 239, 1306–1314. doi:10.1002/dvdy.22224
Pomatto, L. C. D., and Davies, K. J. A. (2017). The role of declining adaptive homeostasis in ageing. J. Physiol. (Lond.) 595, 7275–7309. doi:10.1113/JP275072
Pukkila-Worley, R., Feinbaum, R., Kirienko, N. V., Larkins-Ford, J., Conery, A. L., and Ausubel, F. M. (2012). Stimulation of host immune defenses by a small molecule protects C. elegans from bacterial infection. PLoS Genet. 8, e1002733. doi:10.1371/journal.pgen.1002733
Qi, S., Zheng, H., Chen, C., and Jiang, H. (2019). Du-Zhong (Eucommia ulmoides Oliv.) cortex extract alleviates lead acetate-induced bone loss in rats. Biol. Trace Elem. Res. 187, 172–180. doi:10.1007/s12011-018-1362-6
Qin, D., Tang, Y., Wang, X., Mao, Y., and Feng, Z. (2019). Antagonistic effect of Cuscuta chinensis on a rat model with unilateral cryptorchidism. Med. Sci. Monit. 25, 6727–6735. doi:10.12659/msm.916893
Raizen, D., Song, B. M., Trojanowski, N., and You, Y. J. (2012). Methods for measuring pharyngeal behaviors. WormBook, 1–13. doi:10.1895/wormbook.1.154.1
Restif, C., Ibáñez-Ventoso, C., Vora, M. M., Guo, S., Metaxas, D., and Driscoll, M. (2014). CeleST: computer vision software for quantitative analysis of C. elegans swim behavior reveals novel features of locomotion. PLoS Comput. Biol. 10, e1003702. doi:10.1371/journal.pcbi.1003702
Ryu, D., Mouchiroud, L., Andreux, P. A., Katsyuba, E., Moullan, N., Nicolet-Dit-Félix, A. A., et al. (2016). Urolithin A induces mitophagy and prolongs lifespan in C. elegans and increases muscle function in rodents. Nat. Med. 22, 879–888. doi:10.1038/nm.4132
Sato, K., Yoshiga, T., and Hasegawa, K. (2014). Activated and inactivated immune responses in Caenorhabditis elegans against Photorhabdus luminescens TT01. SpringerPlus 3, 274. doi:10.1186/2193-1801-3-274
Saul, N., Pietsch, K., Menzel, R., and Steinberg, C. E. (2008). Quercetin-mediated longevity in Caenorhabditis elegans: is DAF-16 involved? Mech. Ageing Dev. 129, 611–613. doi:10.1016/j.mad.2008.07.001
Saul, N., Pietsch, K., Stürzenbaum, S. R., Menzel, R., and Steinberg, C. E. (2011). Diversity of polyphenol action in Caenorhabditis elegans: between toxicity and longevity. J. Nat. Prod. 74, 1713–1720. doi:10.1021/np200011a
Saul, N., Pietsch, K., Stürzenbaum, S. R., Menzel, R., and Steinberg, C. E. (2013). Hormesis and longevity with tannins: free of charge or cost-intensive? Chemosphere 93, 1005–1008. doi:10.1016/j.chemosphere.2013.05.069
Scerbak, C., Vayndorf, E. M., Hernandez, A., Mcgill, C., and Taylor, B. E. (2016). Mechanosensory neuron Aging: differential trajectories with Lifespan-Extending Alaskan berry and fungal treatments in Caenorhabditis elegans. Front. Aging Neurosci. 8, 173. doi:10.3389/fnagi.2016.00173
Shin, S., Lee, Y. J., Kim, E. J., Lee, A. S., Kang, D. G., and Lee, H. S. (2011). Effect of Cuscuta chinensis on renal function in ischemia/reperfusion-induced acute renal failure rats. Am. J. Chin. Med. 39, 889–902. doi:10.1142/S0192415X11009287
Somogyvári, M., Gecse, E., and Sőti, C. (2018). DAF-21/Hsp90 is required for C. elegans longevity by ensuring DAF-16/FOXO isoform A function. Sci. Rep. 8, 12048. doi:10.1038/s41598-018-30592-6
Son, H. G., Altintas, O., Kim, E. J. E., Kwon, S., and Lee, S.-J. V. (2019). Age-dependent changes and biomarkers of aging in Caenorhabditis elegans. Aging Cell 18, e12853. doi:10.1111/acel.12853
Stearns, S. C. (1989). Trade-offs in life-history evolution. Funct. Ecol. 3, 259–268. doi:10.2307/2389364
Sugawara, T., and Sakamoto, K. (2020). Quercetin enhances motility in aged and heat-stressed Caenorhabditis elegans nematodes by modulating both HSF-1 activity, and insulin-like and p38-MAPK signalling. PloS One 15, e0238528. doi:10.1371/journal.pone.0238528
Sun, K., Zhao, C., Chen, X. F., Kim, H. K., Choi, B. R., Huang, Y. R., et al. (2013). Ex vivo relaxation effect of Cuscuta chinensis extract on rabbit corpus cavernosum. Asian J. Androl. 15, 134–137. doi:10.1038/aja.2012.124
Sun, S. L., Guo, L., Ren, Y. C., Wang, B., Li, R. H., Qi, Y. S., et al. (2014). Anti-apoptosis effect of polysaccharide isolated from the seeds of Cuscuta chinensis Lam on cardiomyocytes in aging rats. Mol. Biol. Rep. 41, 6117–6124. doi:10.1007/s11033-014-3490-1
Suter, S., and Lucock, M. (2017). Xenohormesis: applying evolutionary principles to contemporary health issues. Explorat. Res. Hypoth. Med. 2, 79–85. doi:10.14218/erhm.2017.00023
Terman, A., and Brunk, U. T. (2006). Oxidative stress, accumulation of biological 'Garbage', and aging. Antioxidants Redox Signal. 8, 197–204. doi:10.1089/ars.2006.8.197
Tissenbaum, H. A. (2015). UsingC. elegansfor aging research. Invertebr. Reprod. Dev. 59, 59–63. doi:10.1080/07924259.2014.940470
Tyne, W., Little, S., Spurgeon, D. J., and Svendsen, C. (2015). Hormesis depends upon the life-stage and duration of exposure: examples for a pesticide and a nanomaterial. Ecotoxicol. Environ. Saf. 120, 117–123. doi:10.1016/j.ecoenv.2015.05.024
Upadhyay, A., Chompoo, J., Taira, N., Fukuta, M., and Tawata, S. (2013). Significant longevity-extending effects of Alpinia zerumbet leaf extract on the life span of Caenorhabditis elegans. Biosci. Biotechnol. Biochem. 77, 217–223. doi:10.1271/bbb.120351
Wang, T. J., An, J., Chen, X. H., Deng, Q. D., and Yang, L. (2014). Assessment of Cuscuta chinensis seeds’ effect on melanogenesis: comparison of water and ethanol fractions in vitro and in vivo. J. Ethnopharmacol. 154, 240–248. doi:10.1016/j.jep.2014.04.016
Wang, Y., Tan, X., Li, S., and Yang, S. (2019). The total flavonoid of Eucommia ulmoides sensitizes human glioblastoma cells to radiotherapy via HIF-α/MMP-2 pathway and activates intrinsic apoptosis pathway. OncoTargets Ther. 12, 5515–5524. doi:10.2147/OTT.S210497
Wei, Y., Li, S., Han, C., Bao, Y., and Shi, W. (2019b). Cuscuta chinensis flavonoids alleviate bisphenol A-induced apoptosis of testicular cells in male mice offspring. Andrologia 51, e13427. doi:10.1111/and.13427
Wei, Y.-Q., Sun, M.-M., and Fang, H.-Y. (2019a). Dienzyme-assisted salting-out extraction of flavonoids from the seeds of Cuscuta chinensis Lam. Ind. Crop. Prod. 127, 232–236. doi:10.1016/j.indcrop.2018.10.068
WHO, (2019). International statistical classification of diseases and related health problems (ICD-11). Geneva, Switzerland: World Health Organization.
Wilson, M. A., Shukitt-Hale, B., Kalt, W., Ingram, D. K., Joseph, J. A., and Wolkow, C. A. (2006). Blueberry polyphenols increase lifespan and thermotolerance in Caenorhabditis elegans. Aging Cell 5, 59–68. doi:10.1111/j.1474-9726.2006.00192.x
Woodruff, G. C., Johnson, E., and Phillips, P. C. (2019). A large close relative of C. elegans is slow-developing but not long-lived. BMC Evol. Biol. 19, 74. doi:10.1186/s12862-019-1388-1
Wuyun, T. N., Wang, L., Liu, H., Wang, X., Zhang, L., Bennetzen, J. L., et al. (2018). The hardy rubber tree genome provides insights into the evolution of polyisoprene biosynthesis. Mol. Plant. 11, 429–442. doi:10.1016/j.molp.2017.11.014
Xing, Y.-F., He, D., Wang, Y., Zeng, W., Zhang, C., Lu, Y., et al. (2019). Chemical constituents, biological functions and pharmacological effects for comprehensive utilization of Eucommia ulmoides Oliver. Food Sci. Hum. Wellness 8, 177–188. doi:10.1016/j.fshw.2019.03.013
Xu, H.-Y., Zhang, Y.-Q., Liu, Z.-M., Chen, T., Lv, C.-Y., Tang, S.-H., et al. (2018a). ETCM: an encyclopaedia of traditional Chinese medicine. Nucleic Acids Res. 47, D976–D982. doi:10.1093/nar/gky987
Xu, J., Hou, H., Hu, J., and Liu, B. (2018b). Optimized microwave extraction, characterization and antioxidant capacity of biological polysaccharides from Eucommia ulmoides Oliver leaf. Sci. Rep. 8, 6561. doi:10.1038/s41598-018-24957-0
Yanase, S., Hartman, P. S., Ito, A., and Ishii, N. (1999). Oxidative stress pretreatment increases the X-radiation resistance of the nematode Caenorhabditis elegans. Mutat. Res. 426, 31–39. doi:10.1016/s0027-5107(99)00079-2
Yang, L., Chen, Q., Wang, F., and Zhang, G. (2011). Antiosteoporotic compounds from seeds of Cuscuta chinensis. J. Ethnopharmacol. 135, 553–560. doi:10.1016/j.jep.2011.03.056
Yang, S., Xu, X., Xu, H., Xu, S., Lin, Q., Jia, Z., et al. (2017). Purification, characterization and biological effect of reversing the kidney-yang deficiency of polysaccharides from semen cuscutae. Carbohydr. Polym. 175, 249–256. doi:10.1016/j.carbpol.2017.07.077
Ye, J., Han, W., Deng, P., Jiang, Y., Liu, M., Li, L., et al. (2019). Comparative transcriptome analysis to identify candidate genes related to chlorogenic acid biosynthesis in Eucommia ulmoides Oliv. Trees (Berl.) 33, 1373–1384. doi:10.1007/s00468-019-01865-y
Zeng, L., Yang, Z., Yun, T., Fan, S., Pei, Z., Chen, Z., et al. (2019). Antiaging effect of a Jianpi-yangwei formula in Caenorhabditis elegans. BMC Compl. Alternative Med. 19, 313. doi:10.1186/s12906-019-2704-4
Zera, A. J., and Harshman, L. G. (2001). The physiology of life history trade-offs in animals. Annu. Rev. Ecol. Systemat. 32, 95–126. doi:10.1146/annurev.ecolsys.32.081501.114006
Zhang, A., Sun, H., and Wang, X. (2013). Recent advances in natural products from plants for treatment of liver diseases. Eur. J. Med. Chem. 63, 570–577. doi:10.1016/j.ejmech.2012.12.062
Zhang, J., Zhang, Q., Liu, G., and Zhang, N. (2019). Therapeutic potentials and mechanisms of the Chinese traditional medicine Danshensu. Eur. J. Pharmacol. 864, 172710. doi:10.1016/j.ejphar.2019.172710
Zhang, R., Liu, Z. G., Li, C., Hu, S. J., Liu, L., Wang, J. P., et al. (2009). Du-Zhong (Eucommia ulmoides Oliv.) cortex extract prevent OVX-induced osteoporosis in rats. Bone 45, 553–559. doi:10.1016/j.bone.2008.08.127
Zhang, R., Pan, Y. L., Hu, S. J., Kong, X. H., Juan, W., and Mei, Q. B. (2014). Effects of total lignans from Eucommia ulmoides barks prevent bone loss in vivo and in vitro. J. Ethnopharmacol. 155, 104–112. doi:10.1016/j.jep.2014.04.031
Zhang, W., Fu, Z.-T., Xie, Y., Duan, Z.-W., Wang, Y., and Fan, R.-H. (2018a). High resolution UPLC-MS/MS method for simultaneous separation and determination of six flavonoids from semen cuscutae extract in rat plasma: application to comparative pharmacokinetic studies in normal and kidney-deficient rats. Nat. Prod. Res. 34, 1446–1451. doi:10.1080/14786419.2018.1511556
Zhang, Y., Mi, D.-Y., Wang, J., Luo, Y.-P., Yang, X., Dong, S., et al. (2018b). Constituent and effects of polysaccharides isolated from Sophora moorcroftiana seeds on lifespan, reproduction, stress resistance, and antimicrobial capacity in Caenorhabditis elegans. Chin. J. Nat. Med. 16, 252–260. doi:10.1016/s1875-5364(18)30055-4
Zhao, Y. L., and Wang, D. Y. (2012). Formation and regulation of adaptive response in nematode Caenorhabditis elegans. Oxid. Med. Cell Longev. 2012, 564093. doi:10.1155/2012/564093
Zhou, J. B., Zheng, Y. L., Zeng, Y. X., Wang, J. W., Pei, Z., and Pang, J. Y. (2018). Marine derived xyloketal derivatives exhibit anti-stress and anti-ageing effects through HSF pathway in Caenorhabditis elegans. Eur. J. Med. Chem. 148, 63–72. doi:10.1016/j.ejmech.2018.02.028
Zhou, Y., Liang, M., Li, W., Li, K., Li, P., Hu, Y., et al. (2009). Protective effects of Eucommia ulmoides Oliv. bark and leaf on amyloid β-induced cytotoxicity. Environ. Toxicol. Pharmacol. 28, 342–349. doi:10.1016/j.etap.2009.05.012
Keywords: C. elegans, Cuscuta chinensis, Eucommia ulmoides, healthspan, traditional Chinese medicine, aging
Citation: Sayed SMA, Siems K, Schmitz-Linneweber C, Luyten W and Saul N (2021) Enhanced Healthspan in Caenorhabditis elegans Treated With Extracts From the Traditional Chinese Medicine Plants Cuscuta chinensis Lam. and Eucommia ulmoides Oliv.. Front. Pharmacol. 12:604435. doi: 10.3389/fphar.2021.604435
Received: 09 September 2020; Accepted: 04 January 2021;
Published: 02 February 2021.
Edited by:
Christian W. Gruber, Medical University of Vienna, AustriaReviewed by:
Tobias Eisenberg, University of Graz, AustriaAdam Matkowski, Wroclaw Medical University, Poland
Copyright © 2021 Sayed, Siems, Schmitz-Linneweber, Luyten and Saul. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Nadine Saul, nadine.saul@gmx.de