ORIGINAL RESEARCH article
Volume 12 - 2021 | https://doi.org/10.3389/fphar.2021.671859
REL-1017 (Esmethadone) Increases Circulating BDNF Levels in Healthy Subjects of a Phase 1 Clinical Study
- 1Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova, Italy
- 2Department of Health Science, University of Milan, Milan, Italy
- 3Department of Medical Biotechnology and Translational Medicine, University of Milan, Milan, Italy
- 4Nephrology Division, Boston Children’s Hospital and Harvard Medical School, Boston, MA, United States
- 5Department of Psychiatry, University of California, San Diego School of Medicine, La Jolla, CA, United States
- 6Neuroscience Education Institute, San Diego, CA, United States
- 7Relmada Therapeutics, Inc., New York, NY, United States
Brain-derived neurotrophic factor (BDNF), a neurotrophin widely expressed in the central nervous system, exhibits important effects on neural plasticity. BDNF has been implicated in the mechanism of action of ketamine, a N-methyl-d-aspartic acid receptor (NMDAR) antagonist with rapid anti-depressant effects in humans. REL-1017 (esmethadone), the d-optical isomer of the racemic mixture d-l-methadone, is devoid of clinically relevant opioid activity at doses expected to exert therapeutic NMDAR antagonistic activity in humans. The present study was conducted to ascertain the effects of oral administration of 25 mg of REL-1017 for 10 days on plasma BDNF in healthy subjects confined to an inpatient unit for a phase 1 clinical trial. We observed an increase in post-treatment BDNF plasma levels compared to pre-treatment levels. Post-treatment, Day 10 BDNF plasma levels ranged from 2 to 17 times pre-treatment levels in the 25 mg REL-1017 treatment group, whereas in the placebo group, BDNF plasma levels remained unchanged (p = 0.028). Diastolic blood pressure decreased significantly in subjects treated with REL-1017, while no effect could be observed in the placebo group. In conclusion, the administration of 25 mg REL-1017 significantly increased BDNF plasma levels and significantly decreased diastolic blood pressure in healthy subjects confined to an inpatient unit for a phase 1 clinical trial.
- REL-1017 induces a significant and rapid increase in plasma BDNF levels in healthy subjects confined to an inpatient unit for a phase 1 clinical study.
- REL-1017 reduced diastolic blood pressure in these subjects.
Brain-derived neurotrophic factor (BDNF) is a neurotrophin that plays a key role in neural plasticity. In the central nervous system (CNS), BDNF is produced by neurons in different brain areas (Miranda et al., 2019) and binds to tyrosine kinase coupled receptors eliciting downstream effects. BDNF production has been implicated in the mechanism of action of antidepressant drugs (Li et al., 2010). In particular, the therapeutic effects of ketamine (Swainson et al., 2019), an established N-methyl-D-aspartic acid receptor (NMDAR) channel blocker with rapid antidepressant effects, are BDNF dependent (Pochwat et al., 2014; Kraus et al., 2019). In patients with treatment-resistant depression (TRD), ketamine rapidly and significantly increased plasma BDNF levels in treatment responders compared to non-responders (Haile et al., 2014). Similar results have also been obtained in preclinical studies, since ketamine and other NMDAR channel blockers produce rapid behavioral antidepressant-like effects in animal models. These effects are dependent on rapid synthesis of BDNF (Li et al., 2010; Autry et al., 2011). Furthermore, it has been observed that subjects carrying the Val66Met (rs6265) single nucleotide polymorphism (SNP), which has been shown to affect intracellular trafficking and secretion of BDNF, are at higher risk for developing major depressive disorder (MDD) compared to Val/Val subjects (Laje et al., 2012). This clinical observation is confirmed by animal studies, since the mouse model carrying this SNP in both alleles (BDNFMet/Met) replicates the phenotypic hallmarks of MDD. Furthermore, these authors demonstrated that BDNF was expressed in mouse brains at normal levels, but its neuronal secretion was defective. From the behavioral point of view, BDNFMet/Met mice subjected to stressful conditions exhibited increased anxiety-related behaviors which were unresponsive to antidepressants (see (Tsai, 2018) and refs therein). Accordingly, Val/Val subjects were more likely to exhibit increased an antidepressant response to ketamine than Met carriers (Laje et al., 2012). Based on these observations, Liu and collaborators (Liu et al., 2012) hypothesized that the reduced antidepressant response to ketamine infusion observed in approximately 30% of treated patients might be related to the Val66Met SNP.
REL-1017 (esmethadone; dextromethadone), the dextro isomer of racemic d-l-methadone, is a low affinity NMDAR channel blocker with a half maximal inhibitor concentration (IC50) in the micromolar range, similarly to ketamine and dextromethorphan (Laurel Gorman et al., 1997; Hanania et al., 2020). In preclinical studies, REL-1017 improved the depressive behavior in rodent models of depression (Fogaça et al., 2019; Hanania et al., 2020) and increased levels of synaptic proteins in the medial prefrontal cortex (Fogaça et al., 2019). Mechanistic studies indicated that the antidepressant-like effects of REL-1017 observed in rodent models were mediated by mammalian target of rapamycin complex 1 (mTORC1) and BDNF induction of neural plasticity (Fogaça et al., 2019). In phase 1 clinical trials, REL-1017 demonstrated favorable safety, tolerability, and pharmacokinetic profiles (De Martin et al., 2018; Bernstein et al., 2019). Therefore, based on preclinical in vivo results (Fogaça et al., 2019) and on in vitro experimental data obtained with ketamine and other NMDAR channel blockers (Kotermanski and Johnson, 2009), we hypothesized that REL-1017 may preferentially block tonically and pathologically hyperactive NMDARs while sparing physiological phasic glutamatergic activity, allowing for improvement of depressive symptoms in the absence of cognitive side effects typical of NMDAR channel blockers. In this context, the down-regulation of excessive Ca2+ influx via tonically and pathologically hyperactive NMDARs would determine downstream effects of enhanced production/release of BDNF and restore BDNF-dependent neural plasticity.
Healthy volunteers undergoing voluntary hospitalization to test new pharmaceutical compounds with potential psychiatric indications likely are subjected to clinically meaningful stress. As with animal models of depression, excessive stress may cause molecular changes that underlie the development of depression, and these molecular changes may be reversed by REL-1017, via BDNF dependent mechanism, potentially determined by blocking excessive Ca2+ influx via hyperactive NMDARs (Fogaça et al., 2019). However, whether the therapeutic effects of REL-1017 in humans are correlated with enhanced production/release of BDNF remains to be determined. Furthermore, low circulating BDNF levels have been associated with coronary artery disease (CAD) (Lee et al., 2017), and a prospective study suggests that low BDNF concentrations predict CAD and higher all-cause mortality (Jiang et al., 2011). Therefore, low BDNF levels may be central to the crosstalk between CNS disorders and cardiovascular homeostasis. Furthermore, effects of stress on blood pressure (BP) have been demonstrated, and it is likely that exposure to stress may be linked to sustained BP elevations (Chrousos, 2009).
In light of these considerations, we evaluated the effects of REL-1017 administration on BDNF plasma levels, and systolic and diastolic BP in healthy volunteers confined to an inpatient unit for a phase 1 clinical study.
Subjects and Study Design
The present study was conducted as part of a single site, randomized, double-blind, placebo-controlled phase 1 clinical trial (ClinicalTrials.gov Identifier: NCT03637361) of 25 mg REL-1017 administered orally for 10 days to healthy volunteers admitted for 14 days to a Clinical Research Unit (CRU) (Bernstein et al., 2019). The 8 subjects, whose demographic characteristics are listed in Table 1, were randomly assigned to the 2 experimental groups, i.e., placebo (2 subjects) and 25 mg of REL-1017 (6 subjects). The demographic and physical characteristics were similar between the two treatment groups. BDNF plasma levels were measured before the first dose (Day 1, predose) and at Days 2, 6, and 10. Blood pressure was measured both 2 and 24 h after the administration of REL-1017.
Measurement of BDNF Plasma Concentrations
Blood sampling and plasma REL-1017 concentrations, as well as BP measurements, were assessed as described by Bernstein et al. (2019). BDNF plasma levels were assessed in blood samples obtained before treatment start (Day 1, predose) and 4 h after administration of a 25 mg-dose of REL-1017 (six patients) or placebo (two patients) on Days 2, 6, and 10. Plasma levels of BDNF were measured with an ELISA kit (Raybiotech, Peachtree Corners, Georgia, United States) following the manufacturer’s instructions. Quantitative determination of BDNF was carried out by standard calibration curves obtained with human recombinant BDNF at concentrations ranging from 0.066 to 16 ng/ml (n = 7), processed following the same protocol used for the plasma samples. As expected, the calibration curves fitted an allosteric sigmoidal equation (r2 ≥ 0.99). We first performed a preliminary study on the subjects’ plasma samples to find the appropriate dilution to obtain BDNF values within the calibration range (1:2 or 1:10 dilutions were selected as appropriate). Each concentration was the result of three independent determinations. Data are presented as mean ± SD or SEM.
Statistical analyses were performed with GraphPad Prism 8.0. Wilcoxon Signed Rank test was used to compare BDNF concentrations before treatment and 4 h after administration of REL-1017 or placebo at Days 2, 6, and 10. We also compared demographic characteristics and BDNF values obtained in placebo and treated subjects with Mann-Whitney test and assessed the existence of a correlation between plasma REL-1017 and BDNF concentrations by the Spearman correlation analysis. A p-value <0.05 was considered statistically significant.
BDNF levels were significantly higher in REL-1017-treated subjects compared to the placebo group starting on Day 2 (p = 0.0357 vs. placebo); no significant difference between the two groups were present at baseline (Figure 1A). We also observed in all REL-1017-treated subjects an increase in BDNF plasma levels post-treatment compared to Day 1 pre-treatment values. The robust increase in BDNF plasma levels was maintained throughout the duration of treatment with REL-1017 (p = 0.028 at Day 2, p = 0.043 at Day 6, and p = 0.028 at Day 10 vs. pre-treatment, respectively). By contrast, BDNF plasma levels remained unchanged in the placebo group (Figures 1C,D). Plasma BDNF levels were significantly correlated with plasma levels of REL-1017 (p < 0.0001, Figure 1B). The lowest Day 10 plasma BDNF increase (less than twice the pre-treatment level) was seen in study subject with the lowest Day 10 REL-1017 level and AUC among all six treated subjects (Subject 6, Supplementary Table S1). We also observed significant changes in systolic and diastolic BP at 2 and 24 h after administration of REL-1017. While the decrease in systolic BP did not reach statistical significance (Figure 2A), a highly significant decrease in diastolic BP was evident in REL-1017 vs. placebo subjects (Figure 2B), both at 2 and 24 h after drug administration.
FIGURE 1. (A) Plasma BDNF concentration in placebo (black line) and REL-1017-treated (red line) subjects. p < 0.05 vs. placebo at the same time point. (B) Spearman correlation between REL-1017 and BDNF plasma concentrations. (C) Percentage changes in BDNF levels in placebo subjects. (D) delta changes in BDNF levels in treated subjects. *p < 0.05 vs. Day 1 (baseline).
FIGURE 2. Changes in systolic (A) and diastolic (B) blood pressure 2 and 24 h after REL-1017 administration. The data represent differences from baseline in BP measurements taken during treatment. ***p < 0.001 and ****p < 0.0001 vs. placebo.
Healthy volunteers confined to an inpatient unit for a phase 1 clinical study are likely subjected to significant psychological stress. In these subjects, psychological stress could activate NMDAR in specific brain circuits, and NMDAR hyperactivation may in turn lead to dysregulated calcium signaling with downregulation of the synthesis and/or release of BDNF, as seen in rodent models of depressive-like behavior (Fogaça et al., 2019). Administration of REL-1017 in animal models rapidly reverses depressive like behavior (Fogaça et al., 2019; Hanania et al., 2020), via BDNF dependent mechanisms (Fogaça et al., 2019) and produced a rapid and robust increase in plasma BDNF in subjects confined to an inpatient for a phase 1 study by nearly 3-fold at Day 2 and Day 6 and more than 5-fold at Day 10. The role of BDNF in the cardiovascular system has been studied extensively and convincing evidence points to BDNF as a protective hormone in cardiovascular homeostasis (Pius-Sadowska and Machaliński, 2017). Furthermore, while the neuropsychiatric phenotypes of individuals carrying the Val66Met SNP have been studied extensively (Glatt and Lee, 2016), the consequences of this polymorphism on cardiovascular function are not completely defined. However, clinical studies demonstrated that subjects carrying Val/Val genotype have a higher risk than Met allele carriers of developing cardiovascular events (Jiang et al., 2017), further strengthening the link between BDNF and cardiovascular health.
Interestingly, BP was reduced in response to REL-1017 treatment. These effects may be due to the blocking effect of REL-1017 on NMDAR channels on neurons in the ventral medial prefrontal cortex, which physiologically activate the cardiac baroreflex response (Lagatta et al., 2018). Alternatively, the hypotensive effect of REL-1017 may be related to NMDAR blockade of neurons that are part of circuits linked to BDNF-dependent antidepressant effects. Val66Met polymorphism has been associated with higher anticipatory cortisol stress response and anxiety in healthy adults (Colzato et al., 2011).
It should be underscored that the data reported in this study were obtained from a retrospective analysis of a limited number of subjects. However, the BDNF increase in treated subjects was quite dramatic. Furthermore, these clinical findings are consistent with preclinical studies demonstrating that REL-1017 exerts an antidepressant-like activity in animal models of depressive-like behavior comparable to that exerted by ketamine and that this antidepressant-like effect may be due to the modulation of neural plasticity via BDNF-dependent mechanisms (Fogaça et al., 2019).
In conclusion, given the demonstrated preclinical efficacy of REL-1017 in murine models of depressive-like behavior and the overall very favorable pharmacokinetic, safety, and tolerability profile from two phase 1 studies (Bernstein et al., 2019), including the lack of psychotomimetic and opioidergic side effects, REL-1017 may represent a true breakthrough for the treatment of depression. The increase in BDNF plasma levels after REL-1017 administration in subjects experiencing a stressful event, could help clarify the molecular mechanisms underlying the therapeutic efficacy of REL-1017 and other NMDAR channel blockers and the pathophysiology of MDD.
Data Availability Statement
The original contributions presented in the study are included in the article/Supplementary Material, further inquiries can be directed to the corresponding author.
The studies involving human participants were reviewed and approved by the INC. Research Toronto. The patients/participants provided their written informed consent to participate in this study.
SDM analyzed the data and wrote the manuscript, DG performed the experiments, FB, PF, NF, and CEI critically revised the manuscript, FF, SS, and PLM conceived the study and revised the manuscript, MP and ST followed and sponsored the clinical trial.
The study was sponsored by Relmada Therapeutics, Incorporated.
Conflict of Interest
SDM: Ongoing research projects sponsored by Relmada Therapeutics. NF: Relationship with Bristol-Mayers, Daiichi-Sankyo, Pharmanutra, and Amryt Pharma. SS: Consultant for Acadia, Alkermes, Allergan, AbbVie, Arbor Pharmaceuticals, Axovant, Axsome, Celgene, Concert, Clearview, EMD Serono, Eisai Pharmaceuticals, Ferring, Impel NeuroPharma, Intra-Cellular Therapies, Ironshore Pharmaceuticals, Janssen, Karuna, Lilly, Lundbeck, Merck, Otsuka, Pfizer, Relmada, Sage Therapeutics, Servier, Shire, Sunovion, Takeda, Taliaz, Teva, Tonix, Tris Pharma, and Viforpharma. Board Member: GenoMind; Served on Speakers bureaus for: Acadia, Lundbeck, Otsuka, Perrigo, Servier, Sunovion, Takeda, Teva, and Vertex; Research and/or grand support from: Acadia, Avanir, Braeburn Pharmaceuticals, Eli Lilly, Intra-Cellular Therapies, Ironshore, ISSWSH, Neurocrine, Otsuka, Shire, Sunovion, and TMS NeuroHealth Centers. CI: Consultant for and owns stock in Relmada Therapeutics. MP: Consultant for Relmada Therapeutics. ST: CEO of Relmada Therapeutics and significant stock ownership. PM: Paid consultant of Relmada Therapeutics, owns stock of Relmada and may earn Royalties from IP licensed to Relmada.
The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fphar.2021.671859/full#supplementary-material
Autry, A. E., Adachi, M., Nosyreva, E., Na, E. S., Los, M. F., Cheng, P.-f., et al. (2011). NMDA Receptor Blockade at Rest Triggers Rapid Behavioural Antidepressant Responses. Nature 475, 91–95. doi:10.1038/nature10130
Bernstein, G., Davis, K., Mills, C., Wang, L., McDonnell, M., Oldenhof, J., et al. (2019). Characterization of the Safety and Pharmacokinetic Profile of D-Methadone, a Novel N-Methyl-D-Aspartate Receptor Antagonist in Healthy, Opioid-Naive Subjects. J. Clin. Psychopharmacol. 39, 226–237. doi:10.1097/JCP.0000000000001035
Chrousos, G. P. (2009). Stress and Disorders of the Stress System. Nat. Rev. Endocrinol. 5, 374–381. doi:10.1038/nrendo.2009.106
Colzato, L. S., Van der Does, A. J. W., Kouwenhoven, C., Elzinga, B. M., and Hommel, B. (2011). BDNF Val66Met Polymorphism Is Associated with Higher Anticipatory Cortisol Stress Response, Anxiety, and Alcohol Consumption in Healthy Adults. Psychoneuroendocrinology 36, 1562–1569. doi:10.1016/j.psyneuen.2011.04.010
De Martin, S., Vitolo, O., Bernstein, G., Alimonti, A., Traversa, A, Inturrisi, C. E., et al. (2018). The NMDAR Antagonist REL-1017 Increases Plasma BDNF Levels in Healthy Volunteers Undergoing a 14-Day In-Patient Phase 1 Study. ACNP 57th Annual Meeting: Poster Session II. Neuropsychopharmacol 43, 228–382.
Fogaça, M. V., Fukumoto, K., Franklin, T., Liu, R.-J., Duman, C. H., Vitolo, O. V., et al. (2019). N-Methyl-D-aspartate Receptor Antagonist D-Methadone Produces Rapid, mTORC1-dependent Antidepressant Effects. Neuropsychopharmacol. 44, 2230–2238. doi:10.1038/s41386-019-0501-x
Glatt, C. E., and Lee, F. S. (2016). Common Polymorphisms in the Age of Research Domain Criteria (RDoC): Integration and Translation. Biol. Psychiatry 79, 25–31. doi:10.1016/j.biopsych.2014.12.020
Haile, C. N., Murrough, J. W., Iosifescu, D. V., Chang, L. C., Al Jurdi, R. K., Foulkes, A., et al. (2014). Plasma Brain Derived Neurotrophic Factor (BDNF) and Response to Ketamine in Treatment-Resistant Depression. Int. J. Neuropsychopharm. 17, 331–336. doi:10.1017/S1461145713001119
Hanania, T., Manfredi, P., Inturrisi, C., and Vitolo, O. V. (2020). The N-Methyl-D-Aspartate Receptor Antagonist D-Methadone Acutely Improves Depressive-like Behavior in the Forced Swim Test Performance of Rats. Exp. Clin. Psychopharmacol. 28, 196–201. doi:10.1037/pha0000310
Jiang, H., Liu, Y., Zhang, Y., and Chen, Z.-Y. (2011). Association of Plasma Brain-Derived Neurotrophic Factor and Cardiovascular Risk Factors and Prognosis in Angina Pectoris. Biochem. Biophysical Res. Commun. 415, 99–103. doi:10.1016/j.bbrc.2011.10.020
Jiang, R., Babyak, M. A., Brummett, B. H., Hauser, E. R., Shah, S. H., Becker, R. C., et al. (2017). Brain-Derived Neurotrophic Factor Rs6265 (Val66Met) Polymorphism Is Associated with Disease Severity and Incidence of Cardiovascular Events in a Patient Cohort. Am. Heart J. 190, 40–45. doi:10.1016/j.ahj.2017.05.002
Kotermanski, S. E., and Johnson, J. W. (2009). Mg2+ Imparts NMDA Receptor Subtype Selectivity to the Alzheimer's Drug Memantine. J. Neurosci. 29, 2774–2779. doi:10.1523/JNEUROSCI.3703-08.2009
Kraus, C., Kadriu, B., Lanzenberger, R., Zarate, C. A., and Kasper, S. (2020). Prognosis and Improved Outcomes in Major Depression: a Review. Foc 18, 220–235. doi:10.1176/appi.focus.18205
Lagatta, D. C., Kuntze, L. B., Ferreira-Junior, N. C., and Resstel, L. B. M. (2018). Medial Prefrontal Cortex TRPV1 and CB1 Receptors Modulate Cardiac Baroreflex Activity by Regulating the NMDA Receptor/nitric Oxide Pathway. Pflugers Arch. - Eur. J. Physiol. 470, 1521–1542. doi:10.1007/s00424-018-2149-5
Laje, G., Lally, N., Mathews, D., Brutsche, N., Chemerinski, A., Akula, N., et al. (2012). Brain-Derived Neurotrophic Factor Val66Met Polymorphism and Antidepressant Efficacy of Ketamine in Depressed Patients. Biol. Psychiatry 72, e27–e28. doi:10.1016/j.biopsych.2012.05.031
Laurel Gorman, A., Elliott, K. J., and Inturrisi, C. E. (1997). The D- and L- Isomers of Methadone Bind to the Non-competitive Site on the N-Methyl-D-Aspartate (NMDA) Receptor in Rat Forebrain and Spinal Cord. Neurosci. Lett. 223, 5–8. doi:10.1016/s0304-3940(97)13391-2
Lee, I.-T., Wang, J.-S., Lee, W.-J., Lin, S.-Y., Fu, C.-P., Liang, K.-W., et al. (2017). The Synergistic Effect of Vascular Cell Adhesion Molecule-1 and Coronary Artery Disease on Brain-Derived Neurotrophic Factor. Clinica Chim. Acta 466, 194–200. doi:10.1016/j.cca.2017.01.026
Li, N., Lee, B., Liu, R.-J., Banasr, M., Dwyer, J. M., Iwata, M., et al. (2010). mTOR-Dependent Synapse Formation Underlies the Rapid Antidepressant Effects of NMDA Antagonists. Science 329, 959–964. doi:10.1126/science.1190287
Liu, R.-J., Lee, F. S., Li, X.-Y., Bambico, F., Duman, R. S., and Aghajanian, G. K. (2012). Brain-derived Neurotrophic Factor Val66Met Allele Impairs Basal and Ketamine-Stimulated Synaptogenesis in Prefrontal Cortex. Biol. Psychiatry 71, 996–1005. doi:10.1016/j.biopsych.2011.09.030
Miranda, M., Morici, J. F., Zanoni, M. B., and Bekinschtein, P. (2019). Brain-Derived Neurotrophic Factor: A Key Molecule for Memory in the Healthy and the Pathological Brain. Front. Cel. Neurosci. 13, 363. doi:10.3389/fncel.2019.00363
Pius-Sadowska, E., and Machaliński, B. (2017). BDNF - A Key Player in Cardiovascular System. J. Mol. Cell Cardiol. 110, 54–60. doi:10.1016/j.yjmcc.2017.07.007
Pochwat, B., Pałucha-Poniewiera, A., Szewczyk, B., Pilc, A., and Nowak, G. (2014). NMDA Antagonists under Investigation for the Treatment of Major Depressive Disorder. Expert Opin. Investig. Drugs 23, 1181–1192. doi:10.1517/13543784.2014.918951
Swainson, J., Thomas, R. K., Archer, S., Chrenek, C., MacKay, M.-A., Baker, G., et al. (2019). Esketamine for Treatment Resistant Depression. Expert Rev. Neurotherapeutics 19, 899–911. doi:10.1080/14737175.2019.1640604
Tsai, S.-J. (2018). Critical Issues in BDNF Val66Met Genetic Studies of Neuropsychiatric Disorders. Front. Mol. Neurosci. 11, 156. doi:10.3389/fnmol.2018.00156
Keywords: esmethadone, BDNF, depression, clinical trial, NMDAR
Citation: De Martin S, Gabbia D, Folli F, Bifari F, Fiorina P, Ferri N, Stahl S, Inturrisi CE, Pappagallo M, Traversa S and Manfredi PL (2021) REL-1017 (Esmethadone) Increases Circulating BDNF Levels in Healthy Subjects of a Phase 1 Clinical Study. Front. Pharmacol. 12:671859. doi: 10.3389/fphar.2021.671859
Received: 24 February 2021; Accepted: 13 April 2021;
Published: 28 April 2021.
Edited by:Li Zhang, National Institutes of Health (NIH), United States
Reviewed by:Cheng Jiang, Yale University, United States
Matea Nikolac Perković, Rudjer Boskovic Institute, Croatia
Copyright © 2021 De Martin, Gabbia, Folli, Bifari, Fiorina, Ferri, Stahl, Inturrisi, Pappagallo, Traversa and Manfredi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Paolo L. Manfredi, PManfredi@relmada.com