Abstract
The concept of proper resolution of inflammation rather than counteracting it, gained a lot of attention in the past few years. Re-assembly of tissue and cell homeostasis as well as establishment of adaptive immunity after inflammatory processes are the key events of resolution. Neutrophiles and macrophages are well described as promotors of resolution, but the role of T cells is poorly reviewed. It is also broadly known that sphingolipids and their imbalance influence membrane fluidity and cell signalling pathways resulting in inflammation associated diseases like inflammatory bowel disease (IBD), atherosclerosis or diabetes. In this review we highlight the role of sphingolipids in T cells in the context of resolution of inflammation to create an insight into new possible therapeutical approaches.
The innate and adaptive immune system
The immune system is a large network consisting of an innate and an adaptive part. Both systems work together and intertwine with each other to defend the body from germs or foreign substances (Figure 1) (Yatim and Lakkis, 2015; Hillion et al., 2020). The skin as part of the innate immune system is the first defence against pathogens and protects our body from foreign entries. When pathogens passed this barrier the innate immune system then activates a cascade of signalling pathways and immune cells from both, the innate and the adaptive, immune systems to fight off the danger (Smith et al., 2019).
FIGURE 1
Pathogens can be neutralized or directly killed by cells of the innate immune system (Macrophages, Natural killer cells etc.), but the scope of function of the innate immune cells goes beyond. Bacterial cell wall components or viral particles are processed by cells of the innate system and presented as antigens to the adaptive immune system. Furthermore, the innate immune cells recruit adaptive immune cells from the blood to the site of inflammation by secretion of chemokines and cytokines.
The adaptive immune system consists of T lymphocytes, B lymphocytes and the antibodies that are produced by the B cells (Buchholz et al., 2016; Bradley and Thomas, 2019; Wang et al., 2020). B cells come from and mature in the bone marrow. They are activated by a subpopulation of T cells (T helper cells) via antigens that bind at their surface immunoglobulin (Ig). Upon activation B cells then release soluble antibodies. The antibody release enables the humoral immune response consisting of pathogen neutralisation, opsonization and complement activation. The complement system enzymatically facilitates lysis and phagocytosis of pathogens (Merle et al., 2015). Antibodies also prevent bacterial adherence including binding to the pathogen (Forthal, 2014).
T cells derive from the bone marrow and are then moved through the blood for maturation in the thymus. They have a broad spectrum of functions that are determined by their surface expression pattern that divides these cells into different subpopulations (Romagnani, 2014). The activation and maturation of those cells is mediated via the T Cell Receptor (TCR). They have to undergo a checkpoint to ensure only non-self-antigen responsive cells are selected (Fife and Bluestone, 2008). Negative selection of T cells removes self-reactive cells from the body (Palmer, 2003), positive selection assures that only T cells with a TCR that can recognize foreign antigens via MHC stimulation will mature (Klein et al., 2014). This process takes places in the thymus and occurs with cells that already express the TCR and both co-receptors Cluster of Differentiation (CD) 4 and 8.
Inflammation processes and proper resolution
Under normal circumstances, the process of inflammation is a mechanism to defend the body from foreign danger like pathogens, toxins or damaged cells. Usually this process is managed within days and finished by resolution of this acute inflammation (Varela et al., 2018). Inflammatory cells, such as macrophages, neutrophils, B cells and T cells, are recruited and send to the site of pathogen infiltration to act against the those pathogens to heal the injury and thereby restoring tissue homeostasis (Figure 1) (Schnoor et al., 2016).
Local, acute inflammation in the tissue is characterized by Galen’s prominent five properties: rubor (redness), tumor (swelling), dolor (pain), calor (heat) and function laesa (impaired function). These characteristics are triggered by the immune cell response to the infectious or sterile damage. Receptors on the surface of immune cells can recognize harmful stimuli via different receptors. Infectious stimuli are so called PAMPs (pathogen-associated molecular patterns) that activate a variety of PRRs (pattern-recognition receptors) in immune and non-immune cells, but additionally endogenous signals also known as DAMPs (danger-associated molecular patterns) can be responsible for inflammatory responses (Walsh et al., 2013; Eppensteiner et al., 2019; Zindel and Kubes, 2020; Murao et al., 2021). Toll-like receptors (TLRs) are one class of these PRR. When they get activated, different inflammatory signalling cascades in immune or non-immune cells are triggered, resulting in the translocation of the nuclear factor 'kappa-light-chain-enhancer' of activated B-cells (NF-κB), activator protein 1 (AP-1) or interferon regulatory factor 3 (IRF3) from cytosol to the nucleus, thereby leading to a transcriptional regulation of the immune response (Kawai and Akira, 2007; Liu W. et al., 2009). The activation of these pathways leads to the release of pro-inflammatory cytokines like Interferon-Gamma (IFN-γ), Interleukin 1β (IL1β) or IL6 and pro-inflammatory lipid mediators like Platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero3-phosphocholine), leukotriens, prostanoids or sphingolipids (Lawrence, 2009; Schauberger et al., 2016; Srivastava and Baig, 2018; Yanai et al., 2018). Interactions of cytokines with their receptors on immune cells activate signalling pathways including NF-κB, mitogen-activated protein kinase (MAPK), januskinase- signal transducers and activators of transcription (JAK-STAT) that promote further cytokine production, proliferation and differentiation of immune cell to specific subtypes (Moens et al., 2013; Fang et al., 2018; Salas et al., 2020). PAF is produced by various cells including neutrophils, eosinophils, endothelial cells and fibroblasts and induces platelet aggregation and leukocyte degranulation and adhesion (Pałgan and Bartuzi, 2015). Leukotrienes (Cysteinyl leukotrienes (CystLTs), leukotriene B4 (LTB4), leukotriene C4 (LTC4)) are produced by leukocytes such as macrophages, eosinophils, basophils and are important for leukocyte influx and vascular permeability (Rådmark et al., 2015; Schauberger et al., 2016). Prostanoids are produced by almost every cell type and are crucial for chemotaxis, activation of immune cells, act on platelet aggregation and vascular smooth muscle cells (Schauberger et al., 2016). S1P (sphingosine-1 phosphate) is the best known pro-inflammatory sphingolipid and involved in chemotaxis as well as activation of various immune cells (see also chapter 6.4) (Sukocheva et al., 2020).
To restore the pre-inflammation status of the tissue, immune cells must be removed from the inflammatory site, inflammatory signalling processes have to be resolved and chemokine gradients have to be diluted to prevent further migration of immune cells (Figure 2). Chemokines at the site of inflammation are cleaved by proteolysis and sequestration (Ye, 2013; Chongsathidkiet et al., 2018; Tao et al., 2019; Sallenave and Guillot, 2020). Neutrophils are the first cells to be egressed from the tissue (Greenlee-Wacker, 2016). They undergo Fas-ligand induced apoptosis via tumor necrosis factor (TNF) receptor interactions that lead to phosphoinositide 3-kinase (PI3K)-activation, they produce reactive oxygen species (ROS) (Salamone et al., 2001; Croker et al., 2011). Macrophages are responsible for the release of Fas-ligand and TNF and the clearance of neutrophils that went apoptotic (Brown and Savill, 1999). During this efferocytosis the pro-inflammatory expression pattern (M1) of macrophages change to an anti-inflammatory pattern (M2) (Kohno et al., 2021). M2 macrophages are characterized by their ability to release immunosuppressive cytokines like IL10, transforming growth factor β (TGFβ) and vascular endothelial growth factor (VEGF) (Orecchioni et al., 2019).
FIGURE 2
Anti-inflammatory signals are beneficial for tissue homeostasis. Stromal cells, macrophages and progenitor or stem cells must work together in order to impede scar formation or fibrosis (Figure 2). TGFβ released from macrophages promote fibroblast differentiation into myofibroblasts through regulation of ECM remodelling (Caja et al., 2018). VEGF signalling leads to angiogenesis and recovery of normal oxygen supply (Shibuya, 2011). Also mesenchymal stem cells (MSC) affect inflammation by the production of growth factors and chemokines that influence macrophage polarization, maturation and migration (Zhao et al., 2020). MSCs promote phagocytosis of macrophages and their differentiation by effecting the metabolism of the macrophages via a prostaglandin E2 (PGE2) dependant mechanism (Vasandan et al., 2016). Polarization of M1 type macrophages to a M2 type can also be enhanced by MSCs (Liao et al., 2020).
Dysfunction or -regulation of these processes can shift acute inflammation to a chronic status and are the cause of many diseases like inflammatory bowel disease (IBD), chronic airway inflammation, stromal keratitis, rheumatoid arthritis and even cancer.
T cell subsets
T cells have a broad spectrum of functions. CD8 expressing T cells are also called cytotoxic T cells because of their capability of killing pathogens and cancer cells. Cytotoxic T cells have to be stimulated by antigen-presenting cells (APC) via MHC class I in order to mature (Kratky et al., 2011). This happens in the nature of an infection and causes these cells to proliferate and fight off intracellular dangers (Cox and Zajac, 2010). CD8 cells are capable of direct killing of infected cells via induction of apoptosis in those cells through the secretion of perforin and granzymes (Voskoboinik et al., 2015). Perforins form pores into the target’s cell membrane and enable the entrance of granzymes (Osińska et al., 2014). Granzymes are a group of serine proteases that cleave viral and cellular proteins of the infected target cells thereby killing it (Trapani, 2001). Apoptotic target cells are then phagocytized by macrophages (Kourtzelis et al., 2020). Another possibility of target cell killing is the induction of apoptosis via Fas-FasL interactions (Yamada et al., 2017). Upon Fas activation a signalling cascade is activated and caspase proteases are induced following apoptosis of these cells (Aouad et al., 2004; Sobrido-Cameán and Barreiro-Iglesias, 2018).
CD4 cells are often characterized as T helper cells (Th). Those cells regulate immune and non-immune cells via the production and release of cytokines and recognize antigens via MHC class II molecules. There is a variety of different T helper cell subsets, that is, described by their cytokine release and transcriptional profile. On the one hand, Th1 cells are prominent to produce IFNγ, TNFβ, TNFα and IL2 and they also express the transcription factor T-bet (T-box expressed in T cells) (Viallard et al., 1999; Szabo et al., 2000; Hwang et al., 2005; Solomou et al., 2006; Kisuya et al., 2019; Artham et al., 2020). On the other hand, TH2 cells do not release IFNγ, but IL4, IL5 and IL13 and they produce the transcription factors GATA binding protein 3 (GATA-3) and c-Maf (musculoaponeurotic fibrosarcoma) (McKenzie et al., 1998; Kanhere et al., 2012; Paul, 2015; Imbratta et al., 2020). Both subpopulations express different chemokine receptors, Th1 cells express C-C chemokine receptor type 5 (CCR5) and CXCR3, TH2 cells express CCR4 and CCR8 (Zingoni et al., 1998; Turner et al., 2007; Yoshie and Matsushima, 2015; Watanabe et al., 2020). Th1 cells are mainly responsible for the recruitment and activation of macrophages by IFNγ release and TH2 cells activate B cells via IL4 and IL13, but also eosinophils via IL5.
There is one additional major subpopulation among the CD4 effector cells, that is, called Th17 cells. Those cells mainly release IL17A to activate macrophages, endothelial and epithelial cells and fibroblasts, as well as CXCL8 to stimulate neutrophils and exhibit pro-inflammatory functions (Pelletier et al., 2010; Li J. et al., 2013; Brembilla et al., 2013; Tabarkiewicz et al., 2015; Zhang et al., 2021; Muench et al., 2022). RAR-related orphan receptor gamma (ROR(γ)t) is a transcription factor, that is, solely expressed by Th17 cells (Steinmetz et al., 2011).
Besides their effector functions in inflammatory processes, there is one specialized CD4 subset, that has a more regulatory function. Regulatory T cells (Tregs) are a subpopulation of CD4 expressing cells that additionally express high amounts of CD25, the alpha-subunit of the IL2 receptor (Kmieciak et al., 2009). They are also often categorized by their expression of the transcription factor forkhead box P3 (FOXP3) which is a prominent Treg marker (Rudensky, 2011). It is important for both, regulatory and developmental pathways in Tregs. Tregs are known for their immune-suppressive function and behaviour (Wan, 2010). The production of the anti-inflammatory cytokine IL10 is one of the main tasks of Tregs and a key player in suppressive function (Ng et al., 2013). The process of self-tolerance is tightly regulated by Tregs and disturbances in this processes lead to autoimmune diseases (Sakaguchi et al., 2008; Yang et al., 2015). Because of their immunosuppressive function, Tregs are a key mediator in resolution of inflammation. They promote macrophage efferocytosis and modulate monocyte macrophage differentiation thereby driving the resolution of inflammation (Weirather et al., 2014; Proto et al., 2018).
T cell activation
The TCR is a heterodimer complex that has two TCR chains and six CD3 chains also connected with different components like Co-receptors, kinases and ligands. Most T cells express the α- and β- TCR chain and are referred to as normal T cells, whereas T cells that express γ- and δ- TCR chain receptors are called γδ- T cells (Nanno et al., 2007). They only make up 0.5–5% of the T lymphocytes (Carding and Egan, 2002). The variable domains of the α and β, γ δ respectively, are responsible for the specificity of the TCR and distinguish the different antigens presented by the Major Histocompatibility complex (MHC). TCRα/TCRβ and TCRγ/TCRδ heterodimers form complexes with the CD3γ/ε and/or CD3δ/ε heterodimer molecules, as well as CD3ζ homodimers (Birnbaum et al., 2014).
T cells can be generally classified via their cell surface molecules CD4 and 8, respectively. Both markers are co-stimulatory proteins of the TCR. They are important mediators of the MHC peptide recognition cascade and they are also important for the maturation process of T cells in the thymus (Tikhonova et al., 2012). They are single molecules with an extracellular and a transmembrane region and an intracellular tail (Leahy, 1995). The intracellular tail enables interaction with the lymphocyte-specific protein tyrosine kinase (LCK) which initiates the signalling cascade upon activation of the TCR (Artyomov et al., 2010). CD4 can recognize MHC class I molecules, whereas CD8 cells bind to MHC class II molecules (Li Y. et al., 2013).
The activation of TCR is only possible when not only CD3 or CD4/8 are stimulated but also CD28 (Linsley and Ledbetter, 1993). It is a cell surface monomeric protein that binds to CD80 and CD86 of APCs in order to activate phosphoinositide-3-kinases (PI3K) (Garçon et al., 2008). PI3K catalyzes the conversion of phosphatdiylinositol-4,5-bisphosphate (PIP2) to phosphatidylinositol-3,4,5-trisphosphate (PIP3) which then activates phospholipase C-γ (PLC-γ) in the TCR activation machinery (Falasca et al., 1998). CD28 also enhances the production of IL4 and IL10 via TCR activation and CD40L binding (Hünig et al., 2010).
Upon stimulation of the TCR CD4/8 mediated LCK activation leads to the cascade of ZAP-70 phosphorylation and subsequent activation of the linker activator of T cells (LAT) (Lo et al., 2018). LAT as well as CD28, activate PI3K to form PIP2, but LAT can also activate PLC-γ directly (Balagopalan et al., 2015). Signalling pathways of TCR lead to NFκB, AP-1 and NFAT activation, which then promotes cell proliferation, differentiation and cell survival (Smith-Garvin et al., 2009).
The organization of the TCR’s activation takes place in so-called lipid rafts, often also called micro-domains or detergent-resistant membranes (DRM) (Zumerle et al., 2017). Lipid rafts are specialized compartments in the cell membrane with a high density of sphingomyelins, cholesterols and glycosphingolipids (Lingwood and Simons, 2010; Simons and Sampaio, 2011; Regen, 2020). They are cellular signalling hubs, bringing receptors closely together, kinases and second messengers thereby transforming a signal from the membrane to intracellular signalling cascades. The assembly of TCR/CD3, CD28, PKCθ and LCK is defined as supramolecular activation cluster (SMAC) with a central region (c-SMAC) and a peripheral region (p-SMAC) (Zumerle et al., 2017).
Insights into sphingolipid metabolism and function
As lipid rafts are characterized as structures enriched in sphingomyelins and glycosphingolipids, the role of sphingolipids (Sph) is very crucial (Bieberich, 2018). Sphs are one of the most important classes of membrane lipids. They owe their name to the mythological sphinx after they were first discovered in brain tissue in the 1870s (Thudichum, 1962). Sphs commonly consist of a sphingoid base, like sphingosine or sphinganine, as backbone, at which via an amid-bound a second fatty acyl chain of different chain length is added. Further, the C1-hydroxygroup could be connected to different charged groups like phosphate, glucose/galactose or phosphocholine, resulting in Ceramide-1-phosphate, Glucosyl-Ceramide (GluCer), Galactosyl-Ceramide (GalCer) or sphingomyelin (SM). GluCer can further be processed to Lactosyl-Ceramide (LacCer) and complex Gangliosides (GMs). A nice overview of the sphingolipid pathway is given in (Rajasagi and Rouse, 2018). The de novo synthesis of ceramides takes place in the Endoplasmatic Reticulum (ER) afterwards ceramides are transported to the Golgi apparatus where the synthesis of complex sphingolipids takes place (Figure 3). The first step of the sphingolipid de novo synthesis is mediated by the serine palmitoyltransferase (SPT) which condensates serine and palmitoyl-CoA to generate 3-ketosphinganine, that is, directly reduced to sphinganine by the 3-ketophinganine reductase. Six isoforms of ceramide synthases (CerS1-6) add a second acyl-chain of different chain length to sphinganine/sphingosine to generate dihydro-ceramides or ceramides, respectively. CerS1 and CerS4 add C18/C20 fatty acids, CerS2 C22-C26, CerS3 C18-C32 and CerS5 and CerS6 use mainly C14/C16 fatty acids as substrate (Figure 3). Ceramide synthases are regulated and expressed tissue specific and each ceramide has definite cellular effects (Brachtendorf et al., 2019 und Wegner et al., 2016). Sphingolipids could also be recycled in the salvage pathway, where sphingosine can be obtained from constitutive degradation of sphingomyelin or glycosphingolipids either at the plasma membrane or in the late endosomes and lysosomes by the action of sphingomyelinases, glycosidases and ceramidases. Sphingosine is then either N-acetylated by CerS to form ceramides, phosphorylated by sphingosine-kinases to sphingosine-1-phosphate (S1P), or degraded to phosphatidylethanolamine and fatty aldehyde by lyases. S1P regulates many cellular functions via binding to G protein-coupled receptors S1P1-5. Those receptors reside in different cell types and tissues and upon their stimulation trigger signalling cascades for proliferation, survival, migration and vaso-regulative pathways (Schwab and Cyster, 2007; Obinata and Hla, 2019). The impact of membrane located sphingolipids on cells depends on their basic structure. Ceramides, for example, can affect cellular pathways and diseases differentially through the length of their fatty acid chains (Grösch et al., 2012). On the one hand, the amphilic properties of short chain ceramides result in membrane permeability and enable them to translocate freely into the cytosol. On the other hand, long chain ceramides stay tightly bound to the membrane and cannot pass as freely. Very long chain ceramides are responsible for the epidermal barrier function and can be found in the skin (Li et al., 2007). Specific lipid and protein interactions in membranes are crucial for signal transduction and can be influenced also by the chain length, saturation of the acyl chain or their polar head groups (Quinn, 2014; Uche et al., 2021). Therefore, the biophysical properties of membrane lipids can facilitate or impede receptor signalling.
FIGURE 3
Sphs control and influence major cellular events such as apoptosis, cell growth and proliferation, signal transduction and differentiation (Cerbón et al., 2018; Xie et al., 2019; Hadas et al., 2020; Jakobi et al., 2020; Loberto et al., 2020; Mignard et al., 2020; Muthusamy et al., 2020; Monasterio et al., 2021; Capolupo et al., 2022). Therefore, a dysregulation of these processes have also an impact on chronic inflammation which we want to highlight in the next sections.
The impact of sphingolipids on T cells and their role in resolution of inflammation
In this chapter we want to give an overview how T cells affect resolution of inflammation and how sphingolipids can influence these mechanisms. As mentioned above, T cells have different functions in inflammation and can either enhance or contribute to the resolution of inflammatory lesions. While T helper cells seem to increase and sustain chronic inflammation, Tregs display an important factor in the resolution process.
Role of sphingolipids in treg development and function
Early study showed that the adoptive transfer of Tregs into immune-deficient mice with Th1 and Th2 induced colitis leads to resolution of inflammation and reappearance of normal intestinal architecture (Table 1) (Mottet et al., 2003). Nowadays, we know that Tregs promote macrophage efferocytosis by production and release of IL13 that activates IL10 production in macrophages (Proto et al., 2018). IL10 release enhances efferocytosis and immunosuppression but can also promote the M1 to M2 macrophage phenotype class switch. As a hallmark of Treg cells the transcriptional program is controlled by Foxp3 that reduces the expression of sphingomyelin synthase 1 (SMS1). This leads to an increase in ceramide level, among others C18-ceramide (Apostolidis et al., 2016). C18-ceramide interferes strongly with the SET protein (originally named inhibitor 2 of protein phosphatase 2 A) which reduces the interaction between SET and PP2A and constraints its inhibitory action on the PP2A complex (Mukhopadhyay et al., 2009). Therefore, Foxp3-mediated suppression of SMS1 results in accumulation of ceramide in Treg cells that leads to activation of the PP2A complex (Apostolidis et al., 2016). PP2A inhibits the mTORC1/AKT pathway in Tregs which is involved in the regulation of cytokine production (IL-2/IL17) and is important for the supressive function of Treg cells (Apostolidis et al., 2016). Treg specific depletion of PP2A led to a multi-organ, lymphoproliferative autoimmune disorder that manifested in mice by the age of 10–14 weeks (Table 1) (Apostolidis et al., 2016).
TABLE 1
| Sphingolipid class | Animal model | Treatment | T Cell population | References |
|---|---|---|---|---|
| - | SCID and rag1-/- | DSS (colitis) | Adoptive Treg transfer led to resolution of colitis | Mottet et al. (2003) |
| Foxp3YFP-crePpp2r1aflox/flox | - | Tregs displayed different metabolic and cytokine profile and did not show immune suppressive activity | Apostolidis et al. (2016) | |
| Sphingomyelins and Ceramides | ASM-/- | - | Tregs were elevated and more activated | Hollmann et al. (2016) |
| Glycosphingolipids | T cell specific Ugcg-/- | DSS (colitis) | Treg levels were decreased and the pathogenesis of colitis was more severe compared to control | Komuro et al. (2022) |
| C22-24 Ceramides | CerS2-/- | Ovalbumin (asthma) | TH2 response was impared and Th17 response was elevated accompanied by higher TCR signalling activity | Shin et al. (2021) |
| Ceramides | CerS6-/- | GVHD | T cell response to alloantigen was impaired and differentiation into Th1 cells was inhibited | Sofi et al. (2017) |
| Ceramides | CerS5-/- | DSS (colitis) | CD8+ cells were reduced and TCR signalling impaired | El-Hindi et al. (2020) |
| Ceramides | T cell specific CerS4-/- | DSS (colitis) | Prolonged TCR activation in CD8 cells | El-Hindi et al. (2022) |
Animal studies, showing the importance of sphingolipids in T cells.
Another mechanism how sphingomyelin influences Tregs is described by Hollmann et al. (Hollmann et al., 2016). They demonstrated that in acid sphingomyelinase (ASMase)-deficient mice a higher number of splenic Tregs could be observed in comparison to control mice (Table1). ASMase is located in endo-lysosomes and catalyzes the hydrolysis of sphingomyeline to phosphorylcholine and ceramide. This enzyme has a pH optimum of 5.0. After certain stimuli ASMase translocates from the lysosome to the plasma membrane which results in the formation of ceramide-enriches membrane platforms. In T cells ASMase is activated after stimulation of CD28 but not after CD3 stimulation (Boucher et al., 1995). In wt mice ASMase activity is higher in Tregs than in CD4+/FoxP3-/CD25- T cells upon CD28 stimulation. This leads to an increase in ceramide content and higher amount of low lipid order membranes in Tregs. In ASMase deficient Tregs (as well as to a lower amount in CD4+/FoxP3-/CD25- T cells) the percentage of low lipid order membranes is reduced, which goes along with a higher sphingomyelin/ceramide ratio, and facilitates T cell activation (Hollmann et al., 2016). In ASMase knockout Treg cells neither CD25 nor IL-10 were increased in comparison to wt Tregs but the expression of CTLA-4 (Cytotoxic T-Lymphocyte-Associated Protein 4) at the membrane is increased that competes with CD28 for the binding to B7 (CD80/CD86) and additionally removes co-stimulatory ligands from the surface of APCs by transendocytosis (Hollmann et al., 2016). Also in a clinical study it has been shown that treatment of depressive patients with the antidrepessant and acid sphingomyelinase inhibitor sertraline leads to an increase in Tregs among CD4+ T cells which was dependent on CD28 activation (Wiese et al., 2021).
Not only sphingomyelin but also glycosylceramide or gangliosides are important for Treg development. The UDP-glucose ceramide glucosyltransferase (UGCG) is the first enzyme in the process of glycosphingolipid biosynthesis. In IBD patients and in a DSS induced colitis mouse model UGCG activity was decreased, and knockdown of UGCG resulted in Treg decrease and CD4 effector cell increase (Table1). In line with this, intravenouse application of nanoparticles loaded with glucosylceramide (GluCer) into DSS treated mice improved colitis symptomes in these mice and enhaced Treg cells in spleen and colon (Komuro et al., 2022). These data indicate that Treg development and function depends on different sphingolipids that impact activation and differentiation of Tregs at various cell stages.
The role of gangliosides in Th17 development
A dysbalance between Th17 and Treg cells that leans toward the pro-inflammatory Th17 phenotype is also one of the hallmarks of inflammation. To resolve the inflammatory site it should also be considered if pro-inflammatory cells could be inhibited by sphingolipids. It has already been shown that the activation of CD4 and CD8 T cells depends on different gangliosides in their membrane (Nagafuku et al., 2012). Especially during the development of Th17 cells from Th0 cells gangliosides (GMs) and LacCer are upregulated and build a specific signature of differentiated CD4+ Th17cells (Sen et al., 2021). The important role of GMs during this differentiation process is supported by the finding that a deficiency of GM3S (GM3 synthase) attenuates the differentiation of CD4+ T cells to Th17 cells (Zhu et al., 2011). As GMs are enriched in lipid rafts changes in their expression are likely responsible for alterations of lipid raft associated protein functions. The differentiation of Th17 cells is strongly dependent on the IL-6 receptor (Glycoprotein 130, GP130) and TGF-β (Figure 4). Both receptors have been shown to be located in lipid rafts and transduce their signal via the STAT and MAPK pathway (Zuo and Chen, 2009; Allen et al., 2014). Allen et al. have shown that Th17 differentiation is dependent on lipid-raft dependent IL-6 responsiveness (Allen et al., 2014). But also TGFβ receptor signalling and endocytosis is dependent on its localisation into specific lipid rafts at the plasma membrane (Le Roy and Wrana, 2005). Future studies will show if alterations in GM can directly affect IL-6 or TGFβ signalling in Th17 cells via disrupting their association within lipid rafts.
FIGURE 4
The impact of ceramides on TCR activity
T cell activation depends on the assembly of different proteins around the TCR in lipid rafts. Cholesterol binds directly to the TCRβ chain facilitating nanoclustering of TCRs independently of antigen binding (Molnár et al., 2012). TCR activation is also affected, when the lipid order is disturbed, for example, by abolishment of neutral SMase 2 (Börtlein et al., 2019). But also ceramides of distinct chain length are important for T cell function. As described above, six mammalian CerS are responsible for the acyl chain length of sphingolipids. CerS2 knockout mice were less sensitive against Ovalbumin-induced allergic asthma which was accompanied by an impaired Th2 response and increased Th17 response. Notably, the increased Th17 response was only visible after TCR stimulation, indicating that loss of very-long chain ceramides in CerS2 knockout mice affects TCR signalling in Th17 cells (Table 1) (Shin et al., 2021). However also CerS4, CerS5 and CerS6 affect TCR signalling, although in different ways. Inhibition of CerS6 in T cells prevents the differentiation into Th1 cells and impairs TCR signal transduction which was related to a reduced co-localization of PKCθ with CD3 at the plasma membrane (Table 1) (Sofi et al., 2017). In CerS5 knockout mice the number of CD3+/CD8+ and Treg cells was reduced in the intra-epithelial lymphocyte fraction of the colon and TCR signalling was impaired (Table 1) (El-Hindi et al., 2020). Instead, depletion of CerS4 in T cells led to a constitutively and prolonged TCR activation (Table1) (El-Hindi et al., 2022). These data indicate that the molecular composition of the membrane around the TCR is important for proper T cell activation and can be influenced by sphingolipids of various chain length.
The impact of S1P on T cell migration and signalling
S1P is a bioactive lipid mediator, that is, generated ubiquitously. S1P is formed from ceramide, which is recycled from sphingomyelin by sphingomyelinases and then converted to sphingosine by ceramidases. Two isoenzymes, sphingosine kinase 1 and 2 (SphK), phosphorylate sphingosine to S1P. Both enzymes are located differently, SphK1 can be found in the cytoplasm and the cell membrane, whereas SphK2 is located mainly in mitochondria, nucleus and the ER. S1P is exported out of cells by transporters (such as spinster homolog 2 (SPNS2)) and binds to chaperones in the vascular system (Obinata and Hla, 2019). S1P is known to control many cellular processes via binding and signalling to the G-coupled receptors S1P1-5. S1P and S1P1-5 are crucial for inflammatory responses. Especially S1P1 is improtant for lymphocyte trafficking and vascular integrity (Schwab and Cyster, 2007). A gradient of S1P is built between lymph organs, tissues and blood. High concentrations of S1P in the thymus and low concentrations in blood inhibits the egress of T cells and low concentrations in the thymus combined with high concentrations in blood facilitate T cell trafficking out of the thymus. Maintenance of high S1P levels in blood is mediated by SphKs, while low levels of S1P in lymphoid organs is obtained by S1P lyase activity. Binding of S1P to its receptors subsequently leads to an internalisation of the receptors. Co-expression of chemokine receptors can influence S1P receptor 1 surface expression. Expression of CCR7 in T cells, for example, leads T cells into lymphnodes and deletion of CCR7 promotes T cell egress (Debes et al., 2005). Upon TCR stimulation CCR7 expression is downregulated which results in more egress of T cells into the blood. S1P1 is then internalised after S1P exposure and T cells become unresponsive to S1P gradient and migration signals. The internalisation and desensitization of S1P1 is very crucial to the S1P response, incomplete S1PR internalization can promote Th17 dependent autoimmune neuro inflammation (Garris et al., 2013). S1P1 deletion in Th17 cells could bear a resistance to EAE, while deletion in Tregs led to autoimmunity in a MS model (Eken et al., 2017). In inflamed tissue cells respond to Interferon release with the upregulation of CD69, a membrane-bound, type II C-lectin receptor (Cibrián and Sánchez-Madrid, 2017). CD69 binds S1P1 and stabilizes a conformation that mimics S1P1 in a ligand-bound state and induces its internalization, resulting in the trapping of naïve T cells in inflammatory tissue (Shiow et al., 2006; Bankovich et al., 2010). S1P1 also delivers an intrinsic negative feedback loop to decrease thymic production of Tregs thereby suppressing Treg activity via Akt-mTOR pathway (Liu G. et al., 2009). MTOR signalling favours Th17 differentiation and suppresses Treg functions by antagonizing important pathways for Treg activity (Liu et al., 2010). Sustained Smad3 activity is important for Foxp3 expression by TGF-β signalling in Tregs and is antagonized by mTOR pathway activated by S1P1 (Liu et al., 2010). Activation of STAT3 induces Th17 differentiation and attenuates Treg development (Garris et al., 2013). In summary, S1P does not only impact T cell migration but also affects the development of Tregs and Th17 cells, favouring the development of Th17 (Figure 4).
Enhancing the resolution process by pharmacological interaction with the sphingolipid pathway
As described above several enzymes of the sphingolipid pathway can influence T-cell function and differentiation and might be new targets for the pharmacological treatment of inflammation.
The repurposing of drugs that are already used for a long time in patient treatment is an auspicious approach to accelerate the approval of drugs for new treatment targets. Inhibition of ASMase by tricyclic antidepressants like amitriptyline, fluoxetine or sertraline is one of these examples (Kornhuber et al., 2008). Amitriptyline accumulates in the late lysosomes (low pH) and inhibits the translocation of ASMase from lysosomes to the plasma membrane which is subsequently inactivated by proteolytic degradation (Beckmann et al., 2014). How this leads to an increase in Tregs in human blood, as mentioned above for sertraline (Wiese et al., 2021), might be explained by the molecular mechanisms that are induced by the inhibition of ASMase. Inhibition of ASMase by amitriptyline or fluoxetine leads to an accumulation of sphingomyelin in lysosomes and Golgi membranes and ceramides in the endoplasmic reticulum. This leads to the induction of autophagy in cells (Gulbins et al., 2018). Th17 cells are highly sensitive to autophagy induction which results in a switch of gene expression with lower IL17 expression level and enhanced Foxp3 expression that converts these cells into Tregs. In accordance with that the Treg phenotype is highly dependent on autophagy and inhibition of autophagy in Tregs leads to increased production of IL17A in these cells (Park et al., 2016; González-Osuna et al., 2022). These data indicate that the induction of autophagy in Th17 cells might be a trigger to switch the Th17 phenotype to a Treg phenotype thereby promoting anti-inflammatory mechanisms that contribute to the resolution of inflammation. Data from animal studies of cystic fibrosis as well as a clinical phase IIb study indicate that amitriptyline improves lung inflammation and function (Table 2), which might be related to the functional switch of T cells (Becker et al., 2010; Nährlich et al., 2013). These data indicate that tricyclic antidepressants might be new treatment option for chronic inflammatory diseases that are characterized by an unbalance between Th17 and Treg cells.
TABLE 2
| Substance | Target | Indication | Clinical phase | Clinical trial No | Status |
|---|---|---|---|---|---|
| Amitriptyline | ASMase | Lung inflammation | II | NCT00515229 | completed |
| Venclustat | GCS | Sandhoff disease (Late-onset GM2) | III | NCT04221451 | a.n. recruiting |
| Venclustat | GCS | Fabry’s disease | III | NCT05206773 | recruiting |
| III | NCT05280548 | recruiting | |||
| Venclustat | GCS | Gaucher’s Disease Type III | III | NCT05222906 | recruiting |
| Venclustat | GCS | Gaucher Disease Type 1/3 | II | NCT02843035 | a.n. recruiting |
| Fingolimod, Ofatumumab, Siponimod | S1P1, S1P3, S1P4, S1P5 CD20, S1P1, S1P5 | Multiple sclerosis | III | NCT04926818 | recruiting |
| Etrasimod | S1P1, S1P4, S1P5 | Ulcerative colitis | II | NCT04607837 | a.n. recruiting |
| II | NCT05061446 | recruiting | |||
| II | NCT05287126 | recruiting | |||
| III | NCT04706793 | a.n. recruiting | |||
| III | NCT04176588 | recruiting | |||
| Etrasimod | S1P1, S1P4, S1P5 | Eosinophilic esophagitis | II | NCT04682639 | a.n.recruiting |
| Etrasimod | S1P1, S1P4, S1P5 | Crohn’s disease | II/III | NCT04173273 | recruiting |
| Ponesimod | S1P1, S1P4, S1P5 | Multiple sclerosis | III | NCT03232073 | a.n. recruiting |
| II | NCT01093326 | a.n. recruiting | |||
| n.s. | NCT03500328 | recruiting | |||
| IV | NCT03535298 | recruiting | |||
| Amiselimod | S1P1 | Colitis | II | NCT04857112 | recruiting |
| Ozanimod | S1P1, S1P5 | Colitis | II/III | NCT05076175 | recruiting |
| III | NCT03915769 | recruiting | |||
| Ozanimod | S1P1, S1P5 | Crohn’s disease | II/III | NCT05470985 | not yet recruiting |
| III | NCT03467958 | recruiting | |||
| Ozanimod | S1P1, S1P5 | Multiple sclerosis | n.s | NCT05245344 | not yet recruiting |
| NCT05028634 | recruiting | ||||
| Siponimod | S1P1, S1P5 | Multiple sclerosis | n.s | NCT04895202 | recruiting |
| n.s | NCT05376579 | recruiting | |||
| IV | NCT04792567 | a.n. recruiting | |||
| IV | NCT04925557 | recruiting |
Ongoing clinical trials with substances that interfere with the sphingolipid pathway.
ns = not specified.
a.n.recruiting = active not recruiting.
Although, GM3S seems to be an additional beneficial target to manipulate the equilibrium between Th17 and Tregs, unfortunately inhibition of this enzyme might have severe side effects as mutations in the ST3GAL5 gene (encodes for GM3S) are associated with severe infantile-onset of neurological symptoms such as progressive microcephaly, intellectual disability, dyskinetic movements, blindness and deafness (Inamori and Inokuchi, 2022). Gangliosides are important lipid components of the myelin sheath in the central and peripheral nervous system. So, the application of substances that inhibit GM3S should be cell type specific. As from right now, no specific GM3S inhibitor is on the market (Yamanaka et al., 2020). However, a glycosyl-ceramide synthase (GCS) inhibitor might be a useful alternative. Venglustat is an inhibitor of GCS and originally developed for the treatment of patients with lysosomal storage disease (Gaucher disease type 3, Fabry disease and GM2 Gangliosidosis, Sandhoff disease, see also clinical trials in Table 2). GCS couples the first glycoside to ceramide resulting in GlyCer, that is, the precursor for all other complex gangliosides, as well as for the GM3S. Fabry patients suffer additionally from airway inflammation. Treatment of these patients with an enzyme replacement therapy using recombinant human α-galactosidase A enzyme, which partial restores the glycosphingolipid level, stabilizes obstructive lung disease and decreased Th17 cells while increase Th1 cells (Odler et al., 2017). As this was only a very small study, further data are needed. The impact of venglustat on the development of Th17 or Tregs has not been investigated in detail so far.
Furthermost progress is made in the pharmacological inhibition of the S1P signaling pathway. Fingolimod (FTY720) is a prodrug, that is, phosphorylated by Sphk2 to its active metabolite fingolimod-phosphate. It is an unselective S1P receptor agonist, with exception of S1P2, and after binding to S1P1 it leads to an internalization and degradation of this receptor. Fingolimod has immunomodulatory effects and is approved for the treatment of multiple sclerosis patients since 2011 (Stepanovska and Huwiler, 2020). Although, Fingolimod shows promising data in animal studies for ulcerative colitis (UC) (Makled et al., 2022), it is not approved for this indication. Instead, Ozanimod, a selective S1P receptor modulator with high affinity to S1P1 and S1P5 is approved for UC patients. A phase III clinical trial shows a significantly higher clinical remission in colitis patients after ozanimod treatment in comparison to placebo treatment (Sandborn et al., 2021). Other selective S1P receptor modulators are in development or used in clinical trials like Etrasimod (selective modulator of S1PR1,4,5, phase III clinical trial in UC patients), Amiselimod (high affinity to S1PR1, clinical trials in Crohn’s disease (CD)), Ponesimod (selective modulator of S1PR1,4,5 tested in clinical trial phase II for psoriasis) (Pérez-Jeldres et al., 2021) (Table 2). FTY720-p, siponimod, etrasimod, ponesimod, ozanimod, and amiselimod-p bind to the orthosteric site of S1P1 and S1P5. They display similar receptor pharmacology and compete for binding at the same site. Therefore, they can be considered interchangeable with one another (Selkirk et al., 2022). Furthermore, current clinical trials compare the safety and efficacy of combination therapy combining fingolimod, siponimod and ofatumumab (anti-CD20 antibody) in pediatric patients with multiple sclerosis (Table 2). A very comprehensive overview about S1PR modulators in clinical studies is given in (Stepanovska and Huwiler 2020).
These data indicate, that the pharmacological interaction with S1P signaling to influence T cell migration or function is a promising approach to treat inflammatory conditions.
Conclusion
The resolution of inflammation gained a lot of attention in the past few years as an active process of re-establishing tissue-homeostasis and resolving inflammatory processes. Tregs regulate macrophage responses, immunosuppressive cytokine release and restoration of tissue homeostasis whereas pro-inflammatory Th1/2/17 cells promote inflammation. In this review we highlighted how sphingolipids can interfere with the differentiation and activation process of T cells. A particular sphingolipid composition of the membrane seems to be required for the different subsets of T cells and the formation of variable immunological synapses. For this reason sphingolipids influence diverse chronic inflammatory diseases that are driven by Th1 or Th2 cell response, and an imbalance between Th17 and Tregs such as in rheumatoid arthritis (Tsukuda et al., 2012) or IBD patients (Komuro et al., 2022) and are important for the resolution of inflammation. So, in future studies enzymes of the sphingolipid pathway as well as sphingolipids themselves should be considered as new possible targets to boost resolving processes and to dampen pro-inflammatory mechanisms. Ongoing clinical trials with various sphingolipid modulators might shed light on the utility of these substances in different inflammatory diseases. However, to proof if anti-inflammatory effects are mediated by the impact of these substances on T cells, future studies should also investigate the T cell subtypes in blood after treatment of patients with sphingolipid modulators. Additionally, investigating the effect of sphingolipid inhibitors on primary human blood cells in vitro using lipidomics, transcriptomics and characterizing released cytokines by cytometric bead array assay (CBA), might give a hint how these substances influence T cell subtypes and which signaling pathways are involved in the resolution of inflammation.
Statements
Author contributions
JH and SG structured and wrote the paper, JH and NM concepted and designed the figures.
Funding
The Work was supported by the Deutsche Forschungsgemeinschaft (DFG) GR2011/7-1 and SFB1039.
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
References
1
AllenM. J.FanY-Y.MonkJ. M.HouT. Y.BarhoumiR.McMurrayD. N.et al (2014). n-3 PUFAs reduce T-helper 17 cell differentiation by decreasing responsiveness to interleukin-6 in isolated mouse splenic CD4⁺ T cells. J. Nutr.144, 1306–1313. 10.3945/jn.114.194407
2
AouadS. M.CohenL. Y.Sharif-AskariE.HaddadE. K.AlamA.SekalyR-P. (2004). Caspase-3 is a component of Fas death-inducing signaling complex in lipid rafts and its activity is required for complete caspase-8 activation during Fas-mediated cell death. J. Immunol.172, 2316–2323. 10.4049/jimmunol.172.4.2316
3
ApostolidisS. A.Rodríguez-RodríguezN.Suárez-FueyoA.DioufaN.OzcanE.CrispínJ. C.et al (2016). Phosphatase PP2A is requisite for the function of regulatory T cells. Nat. Immunol.17, 556–564. 10.1038/ni.3390
4
ArthamS.VermaA.AlwhaibiA.AdilM. S.ManicassamyS.MunnD. H.et al (2020). Delayed Akt suppression in the lipopolysaccharide-induced acute lung injury promotes resolution that is associated with enhanced effector regulatory T cells. Am. J. Physiol. Lung Cell Mol. Physiol.318, L750–L761. 10.1152/ajplung.00251.2019
5
ArtyomovM. N.LisM.DevadasS.DavisM. M.ChakrabortyA. K. (2010). CD4 and CD8 binding to MHC molecules primarily acts to enhance Lck delivery. Proc. Natl. Acad. Sci. U. S. A.107, 16916–16921. 10.1073/pnas.1010568107
6
BalagopalanL.KortumR. L.CoussensN. P.BarrV. A.SamelsonL. E. (2015). The linker for activation of T cells (LAT) signaling hub: From signaling complexes to microclusters. J. Biol. Chem.290, 26422–26429. 10.1074/jbc.R115.665869
7
BankovichA. J.ShiowL. R.CysterJ. G. (2010). CD69 suppresses sphingosine 1-phosophate receptor-1 (S1P1) function through interaction with membrane helix 4. J. Biol. Chem.285, 22328–22337. 10.1074/jbc.M110.123299
8
BeckerK. A.RiethmüllerJ.LüthA.DöringG.KleuserB.GulbinsE. (2010). Acid sphingomyelinase inhibitors normalize pulmonary ceramide and inflammation in cystic fibrosis. Am. J. Respir. Cell Mol. Biol.42, 716–724. 10.1165/rcmb.2009-0174OC
9
BeckmannN.SharmaD.GulbinsE.BeckerK. A.EdelmannB. (2014). Inhibition of acid sphingomyelinase by tricyclic antidepressants and analogons. Front. Physiol.5, 331. 10.3389/fphys.2014.00331
10
BieberichE. (2018). Sphingolipids and lipid rafts: Novel concepts and methods of analysis. Chem. Phys. Lipids216, 114–131. 10.1016/j.chemphyslip.2018.08.003
11
BirnbaumM. E.BerryR.HsiaoY-S.ChenZ.Shingu-VazquezM. A.YuX.et al (2014). Molecular architecture of the αβ T cell receptor-CD3 complex. Proc. Natl. Acad. Sci. U. S. A.111, 17576–17581. 10.1073/pnas.1420936111
12
BörtleinC.SchumacherF.KleuserB.DölkenL.AvotaE. (2019). Role of neutral sphingomyelinase-2 (NSM 2) in the control of T cell plasma membrane lipid composition and cholesterol homeostasis. Front. Cell Dev. Biol.7, 226. 10.3389/fcell.2019.00226
13
BoucherL-M.WiegmannK.FüttererA.PfefferK.MachleidtT.SchützeS.et al (1995). CD28 signals through acidic sphingomyelinase. J. Exp. Med.181, 2059–6810. 10.1084/jem.181.6.2059
14
BradleyP.ThomasP. G. (2019). Using T cell receptor repertoires to understand the principles of adaptive immune recognition. Annu. Rev. Immunol.37, 547–570. 10.1146/annurev-immunol-042718-041757
15
BrembillaN. C.MontanariE.TruchetetM-E.RaschiE.MeroniP.ChizzoliniC. (2013). Th17 cells favor inflammatory responses while inhibiting type I collagen deposition by dermal fibroblasts: Differential effects in healthy and systemic sclerosis fibroblasts. Arthritis Res. Ther.15, R151. 10.1186/ar4334
16
BrownS. B.SavillJ. (1999). Phagocytosis triggers macrophage release of Fas ligand and induces apoptosis of bystander leukocytes. J. Immunol.162, 480–485.
17
BuchholzV. R.SchumacherT. N. M.BuschD. H. (2016). T cell fate at the single-cell level. Annu. Rev. Immunol.34, 65–92. 10.1146/annurev-immunol-032414-112014
18
CajaL.DituriF.MancarellaS.Caballero-DiazD.MoustakasA.GiannelliG.et al (2018). TGF-Β and the tissue microenvironment: Relevance in fibrosis and cancer. Int. J. Mol. Sci.19. 10.3390/ijms19051294
19
CapolupoL.KhvenI.LedererA. R.MazzeoL.GlouskerG.HoS.et al (2022). Sphingolipids control dermal fibroblast heterogeneity. Science376, eabh1623. 10.1126/science.abh1623
20
CardingS. R.EganP. J. (2002). Gammadelta T cells: Functional plasticity and heterogeneity. Nat. Rev. Immunol.2, 336–345. 10.1038/nri797
21
CerbónJ.Baranda-AvilaN.Falcón-MuñozA.Camacho-ArroyoI.CerbónM. (2018). Sphingolipid synthesis and role in uterine epithelia proliferation. Reproduction156, 173–183.
22
ChongsathidkietP.JacksonC.KoyamaS.LoebelF.CuiX.FarberS. H.et al (2018). Sequestration of T cells in bone marrow in the setting of glioblastoma and other intracranial tumors. Nat. Med.24, 1459–1468. 10.1038/s41591-018-0135-2
23
CibriánD.Sánchez-MadridF. (2017). CD69: From activation marker to metabolic gatekeeper. Eur. J. Immunol.47, 946–953. 10.1002/eji.201646837
24
CoxM. A.ZajacA. J. (2010). Shaping successful and unsuccessful CD8 T cell responses following infection. J. Biomed. Biotechnol.2010, 159152. 10.1155/2010/159152
25
CrokerB. A.O'DonnellJ. A.NowellC. J.MetcalfD.DewsonG.CampbellK. J.et al (2011). Fas-mediated neutrophil apoptosis is accelerated by bid, bak, and bax and inhibited by bcl-2 and mcl-1. Proc. Natl. Acad. Sci. U. S. A.108, 13135–13140. 10.1073/pnas.1110358108
26
DebesG. F.ArnoldC. N.YoungA. J.KrautwaldS.LippM.HayJ. B.et al (2005). Chemokine receptor CCR7 required for T lymphocyte exit from peripheral tissues. Nat. Immunol.6, 889–9410. 10.1038/ni1238
27
EkenA.DuhenR.SinghA. K.FryM.BucknerJ. H.KitaM.et al (2017). S1P1 deletion differentially affects TH17 and Regulatory T cells. Sci. Rep.7, 12905. 10.1038/s41598-017-13376-2
28
El-HindiK.BrachtendorfS.HartelJ. C.OertelS.BirodK.MerzN.et al (2022). T-Cell-Specific CerS4 depletion prolonged inflammation and enhanced tumor burden in the AOM/DSS-Induced CAC model. Int. J. Mol. Sci.23. 10.3390/ijms23031866
29
El-HindiK.BrachtendorfS.HartelJ. C.OertelS.BirodK.TrautmannS.et al (2020). Ceramide synthase 5 deficiency aggravates dextran sodium sulfate-induced colitis and colon carcinogenesis and impairs T-cell activation. Cancers (Basel)12. 10.3390/cancers12071753
30
EppensteinerJ.KwunJ.ScheuermannU.BarbasA.LimkakengA. T.KuchibhatlaM.et al (2019). Damage- and pathogen-associated molecular patterns play differential roles in late mortality after critical illness. JCI Insight4. 10.1172/jci.insight.127925
31
FalascaM.LoganS. K.LehtoV. P.BaccanteG.LemmonM. A.SchlessingerJ. (1998). Activation of phospholipase C gamma by PI 3-kinase-induced PH domain-mediated membrane targeting. EMBO J.17, 414–2210. 10.1093/emboj/17.2.414
32
FangP.LiX.DaiJ.ColeL.CamachoJ. A.ZhangY.et al (2018). Immune cell subset differentiation and tissue inflammation. J. Hematol. Oncol.11, 97. 10.1186/s13045-018-0637-x
33
FifeB. T.BluestoneJ. A. (2008). Control of peripheral T-cell tolerance and autoimmunity via the CTLA-4 and PD-1 pathways. Immunol. Rev.224, 166–182. 10.1111/j.1600-065X.2008.00662.x
34
ForthalD. M. (2014). Functions of antibodies. Microbiol. Spectr.2, AID–201410. 10.1128/microbiolspec.AID-0019-2014
35
GarçonF.PattonD. T.EmeryJ. L.HirschE.RottapelR.SasakiT.et al (2008). CD28 provides T-cell costimulation and enhances PI3K activity at the immune synapse independently of its capacity to interact with the p85/p110 heterodimer. blood111, 1464–1471. 10.1182/blood-2007-08-108050
36
GarrisC. S.WuL.AcharyaS.AracA.BlahoV. A.HuangY.et al (2013). Defective sphingosine 1-phosphate receptor 1 (S1P1) phosphorylation exacerbates TH17-mediated autoimmune neuroinflammation. Nat. Immunol.14, 1166–1172. 10.1038/ni.2730
37
González-OsunaL.Sierra-CristanchoA.CafferataE. A.Melgar-RodríguezS.RojasC.CarvajalP.et al (2022). Senescent CD4+CD28− T lymphocytes as a potential driver of Th17/treg imbalance and alveolar bone resorption during periodontitis. Ijms23, 2543. 10.3390/ijms23052543
38
Greenlee-WackerM. C. (2016). Clearance of apoptotic neutrophils and resolution of inflammation. Immunol. Rev.273, 357–370. 10.1111/imr.12453
39
GröschS.SchiffmannS.GeisslingerG. (2012). Chain length-specific properties of ceramides. Prog. Lipid Res.51, 50–62. 10.1016/j.plipres.2011.11.001
40
GulbinsA.SchumacherF.BeckerK. A.WilkerB.SoddemannM.BoldrinF.et al (2018). Antidepressants act by inducing autophagy controlled by sphingomyelin-ceramide. Mol. Psychiatry23, 2324–2346. 10.1038/s41380-018-0090-9
41
HadasY.VincekA. S.YoussefE.ŻakM. M.ChepurkoE.SultanaN.et al (2020). Altering sphingolipid metabolism attenuates cell death and inflammatory response after myocardial infarction. Circulation141, 916–930. 10.1161/CIRCULATIONAHA.119.041882
42
HillionS.ArleevskayaM. I.BlancoP.BordronA.BrooksW. H.CesbronJ. Y.et al (2020). The innate part of the adaptive immune system. Clin. Rev. Allergy Immunol.58, 151–154. 10.1007/s12016-019-08740-1
43
HollmannC.WernerS.AvotaE.ReuterD.JaptokL.KleuserB.et al (2016). Inhibition of acid sphingomyelinase allows for selective targeting of CD4+ conventional versus Foxp3+ regulatory T cells. J. Immunol.197, 3130–3141. 10.4049/jimmunol.1600691
44
HünigT.LühderF.ElfleinK.GogishviliT.FröhlichM.GulerR.et al (2010). CD28 and IL-4: Two heavyweights controlling the balance between immunity and inflammation. Med. Microbiol. Immunol.199, 239–246. 10.1007/s00430-010-0156-z
45
HwangE. S.HongJ-H.GlimcherL. H. (2005). IL-2 production in developing Th1 cells is regulated by heterodimerization of RelA and T-bet and requires T-bet serine residue 508. J. Exp. Med.202, 1289–1300. 10.1084/jem.20051044
46
ImbrattaC.HusseinH.AndrisF.VerdeilG. (2020). c-MAF, a Swiss army knife for tolerance in lymphocytes. Front. Immunol.11, 206. 10.3389/fimmu.2020.00206
47
InamoriK-I.InokuchiJ-i. (2022). Ganglioside GM3 synthase deficiency in mouse models and human patients. Int. J. Mol. Sci.23. 10.3390/ijms23105368
48
JakobiK.BeyerS.KochA.ThomasD.SchwalmS.ZeuzemS.et al (2020). Sorafenib treatment and modulation of the sphingolipid pathway affect proliferation and viability of hepatocellular carcinoma in vitro. Int. J. Mol. Sci.21. 10.3390/ijms21072409
49
KanhereA.HertweckA.BhatiaU.GökmenM. R.PeruchaE.JacksonI.et al (2012). T-bet and GATA3 orchestrate Th1 and Th2 differentiation through lineage-specific targeting of distal regulatory elements. Nat. Commun.3, 1268. 10.1038/ncomms2260
50
KawaiT.AkiraS. (2007). Signaling to NF-kappaB by toll-like receptors. Trends Mol. Med.13, 460–469. 10.1016/j.molmed.2007.09.002
51
KisuyaJ.ChemtaiA.RaballahE.KeterA.OumaC. (2019). The diagnostic accuracy of Th1 (IFN-γ, TNF-α, and IL-2) and Th2 (IL-4, IL-6 and IL-10) cytokines response in AFB microscopy smear negative PTB- HIV co-infected patients. Sci. Rep.9, 2966. 10.1038/s41598-019-39048-x
52
KleinL.KyewskiB.AllenP. M.HogquistK. A. (2014). Positive and negative selection of the T cell repertoire: What thymocytes see (and don't see). Nat. Rev. Immunol.14, 377–391. 10.1038/nri3667
53
KmieciakM.GowdaM.GrahamL.GodderK.BearH. D.MarincolaF. M.et al (2009). Human T cells express CD25 and Foxp3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function. J. Transl. Med.7, 89. 10.1186/1479-5876-7-89
54
KohnoK.Koya-MiyataS.HarashimaA.TsukudaT.KatakamiM.AriyasuT.et al (2021). Inflammatory M1-like macrophages polarized by NK-4 undergo enhanced phenotypic switching to an anti-inflammatory M2-like phenotype upon co-culture with apoptotic cells. J. Inflamm.18, 2. 10.1186/s12950-020-00267-z
55
KomuroM.NaganeM.EndoR.NakamuraT.MiyamotoT.NiwaC.et al (2022). Glucosylceramide in T cells regulates the pathology of inflammatory bowel disease. Biochem. Biophys. Res. Commun.599, 24–30. 10.1016/j.bbrc.2022.02.004
56
KornhuberJ.TripalP.ReichelM.TerflothL.BleichS.WiltfangJ.et al (2008). Identification of new functional inhibitors of acid sphingomyelinase using a structure-property-activity relation model. J. Med. Chem.51, 219–237. 10.1021/jm070524a
57
KourtzelisI.HajishengallisG.ChavakisT. (2020). Phagocytosis of apoptotic cells in resolution of inflammation. Front. Immunol.11, 553. 10.3389/fimmu.2020.00553
58
KratkyW.Reis e SousaC.OxeniusA.SpörriR. (2011). Direct activation of antigen-presenting cells is required for CD8+ T-cell priming and tumor vaccination. Proc. Natl. Acad. Sci. U. S. A.108, 17414–17419. 10.1073/pnas.1108945108
59
LawrenceT. (2009). The nuclear factor NF-kappaB pathway in inflammation. Cold Spring Harb. Perspect. Biol.1, a001651. 10.1101/cshperspect.a001651
60
Le RoyC.WranaJ. L. (2005). Clathrin- and non-clathrin-mediated endocytic regulation of cell signalling. Nat. Rev. Mol. Cell Biol.6, 112–126. 10.1038/nrm1571
61
LeahyD. J. (1995). A structural view of CD4 and CD8. FASEB J.9, 17–25. 10.1096/fasebj.9.1.7821755
62
LiJ.HsuH-C.MountzJ. D. (2013a). The dynamic duo-inflammatory M1 macrophages and Th17 cells in rheumatic diseases. J. Orthop. Rheumatol.1, 4. 10.13188/2334-2846.1000002
63
LiW.SandhoffR.KonoM.ZerfasP.HoffmannV.DingB. C-H.et al (2007). Depletion of ceramides with very long chain fatty acids causes defective skin permeability barrier function, and neonatal lethality in ELOVL4 deficient mice. Int. J. Biol. Sci.3, 120–128. 10.7150/ijbs.3.120
64
LiY.YinY.MariuzzaR. A. (2013b). Structural and biophysical insights into the role of CD4 and CD8 in T cell activation. Front. Immunol.4, 206. 10.3389/fimmu.2013.00206
65
LiaoY.LiG.ZhangX.HuangW.XieD.DaiG.et al (2020). Cardiac Nestin+ mesenchymal stromal cells enhance healing of ischemic heart through periostin-mediated M2 macrophage polarization. Mol. Ther.28, 855–873. 10.1016/j.ymthe.2020.01.011
66
LingwoodD.SimonsK. (2010). Lipid rafts as a membrane-organizing principle. Science327, 46–50. 10.1126/science.1174621
67
LinsleyP. S.LedbetterJ. A. (1993). The role of the CD28 receptor during T cell responses to antigen. Annu. Rev. Immunol.11, 191–212. 10.1146/annurev.iy.11.040193.001203
68
LiuG.BurnsS.HuangG.BoydK.ProiaR. L.FlavellR. A.et al (2009a). The receptor S1P1 overrides regulatory T cell-mediated immune suppression through Akt-mTOR. Nat. Immunol.10, 769–777. 10.1038/ni.1743
69
LiuG.YangK.BurnsS.ShresthaS.ChiH. (2010). The S1P(1)-mTOR axis directs the reciprocal differentiation of T(H)1 and T(reg) cells. Nat. Immunol.11, 1047–1056. 10.1038/ni.1939
70
LiuW.OuyangX.YangJ.LiuJ.LiQ.GuY.et al (2009b). AP-1 activated by toll-like receptors regulates expression of IL-23 p19. J. Biol. Chem.284, 24006–24016. 10.1074/jbc.M109.025528
71
LoW-L.ShahN. H.AhsanN.HorkovaV.StepanekO.SalomonA. R.et al (2018). Lck promotes Zap70-dependent LAT phosphorylation by bridging Zap70 to LAT. Nat. Immunol.19, 733–741. 10.1038/s41590-018-0131-1
72
LobertoN.ManciniG.BassiR.CarsanaE. V.TamaniniA.PedemonteN.et al (2020). Sphingolipids and plasma membrane hydrolases in human primary bronchial cells during differentiation and their altered patterns in cystic fibrosis. Glycoconj J.37, 623–633. 10.1007/s10719-020-09935-x
73
MakledM. N.SerryaM. S.El-SheakhA. R. (2022). Fingolimod ameliorates acetic acid-induced ulcerative colitis: An insight into its modulatory impact on pro/anti-inflammatory cytokines and AKT/mTOR signalling. Basic Clin. Pharmacol. Toxicol.130, 569–580. 10.1111/bcpt.13720
74
McKenzieG. J.BancroftA.GrencisR. K.McKenzieA. N. J. (1998). A distinct role for interleukin-13 in Th2-cell-mediated immune responses. Curr. Biol.8, 339–4210. 10.1016/s0960-9822(98)70134-4
75
MerleN. S.ChurchS. E.Fremeaux-BacchiV.RoumeninaL. T. (2015). Complement system Part I - molecular mechanisms of activation and regulation. Front. Immunol.6, 262. 10.3389/fimmu.2015.00262
76
MignardV.DuboisN.LanoéD.JoallandM-P.OliverL.PecqueurC.et al (2020). Sphingolipid distribution at mitochondria-associated membranes (MAMs) upon induction of apoptosis. J. Lipid Res.61, 1025–1037. 10.1194/jlr.RA120000628
77
MoensU.KostenkoS.SveinbjørnssonB. (2013). The role of mitogen-activated protein kinase-activated protein kinases (MAPKAPKs) in inflammation. Genes (Basel)4, 101–133. 10.3390/genes4020101
78
MolnárE.SwamyM.HolzerM.Beck-GarcíaK.WorchR.ThieleC.et al (2012). Cholesterol and sphingomyelin drive ligand-independent T-cell antigen receptor nanoclustering. J. Biol. Chem.287, 42664–42674. 10.1074/jbc.M112.386045
79
MonasterioB. G.Jiménez-RojoN.García-ArribasA. B.RiezmanH.GoñiF. M.AlonsoA. (2021). CHO/LY-B cell growth under limiting sphingolipid supply: Correlation between lipid composition and biophysical properties of sphingolipid-restricted cell membranes. FASEB J.35, e21657. 10.1096/fj.202001879RR
80
MottetC.UhligH. H.PowrieF. (2003). Cutting edge: Cure of colitis by CD4+CD25+ regulatory T cells. J. Immunol.170, 3939–3943. 10.4049/jimmunol.170.8.3939
81
MuenchD. E.SunZ.SharmaA.TangC.CramptonJ. S.LaoC.et al (2022). A pathogenic Th17/cd38+ macrophage feedback loop drives inflammatory arthritis through TNF-α. J. Immunol.208, 1315–1328. 10.4049/jimmunol.2101025
82
MukhopadhyayA.SaddoughiS. A.SongP.SultanI.PonnusamyS.SenkalC. E.et al (2009). Direct interaction between the inhibitor 2 and ceramide via sphingolipid-protein binding is involved in the regulation of protein phosphatase 2A activity and signaling. FASEB J.23, 751–763. 10.1096/fj.08-120550
83
MuraoA.AzizM.WangH.BrennerM.WangP. (2021). Release mechanisms of major DAMPs. Apoptosis26, 152–162. 10.1007/s10495-021-01663-3
84
MuthusamyT.CordesT.HandzlikM. K.YouLeLimE. W.GengatharanJ.et al (2020) Serine restriction alters sphingolipid diversity to constrain tumour growth. Nature586:790–795. 10.1038/s41586-020-2609-x
85
NagafukuM.OkuyamaK.OnimaruY.SuzukiA.OdagiriY.YamashitaT.et al (2012). CD4 and CD8 T cells require different membrane gangliosides for activation. Proc. Natl. Acad. Sci. U. S. A.109, E336–E342. 10.1073/pnas.1114965109
86
NährlichL.MainzJ. G.AdamsC.EngelC.HerrmannG.IchevaV.et al (2013). Therapy of CF-patients with amitriptyline and placebo--a randomised, double-blind, placebo-controlled phase IIb multicenter, cohort-study. Cell Physiol. Biochem.31, 505–512. 10.1159/000350071
87
NannoM.ShioharaT.YamamotoH.KawakamiK.IshikawaH. (2007). Γδ T cells: Firefighters or fire boosters in the front lines of inflammatory responses. Immunol. Rev.215, 103–113. 10.1111/j.1600-065X.2006.00474.x
88
NgT. H. S.BrittonG. J.HillE. V.VerhagenJ.BurtonB. R.WraithD. C. (2013). Regulation of adaptive immunity; the role of interleukin-10. Front. Immunol.4, 129. 10.3389/fimmu.2013.00129
89
ObinataH.HlaT. (2019). Sphingosine 1-phosphate and inflammation. Int. Immunol.31, 617–625. 10.1093/intimm/dxz037
90
OdlerB.CsehÁ.ConstantinT.FeketeG.LosonczyG.TamásiL.et al (2017). Long time enzyme replacement therapy stabilizes obstructive lung disease and alters peripheral immune cell subsets in Fabry patients. Clin. Respir. J.11, 942–950. 10.1111/crj.12446
91
OrecchioniM.GhoshehY.PramodA. B.LeyK. (2019). Macrophage polarization: Different gene signatures in M1(LPS+) vs. Classically and M2(LPS-) vs. Alternatively activated macrophages. Front. Immunol.10, 1084. 10.3389/fimmu.2019.01084
92
OsińskaI.PopkoK.DemkowU. (2014). Perforin: An important player in immune response. Cent. Eur. J. Immunol.39, 109–115. 10.5114/ceji.2014.42135
93
PałganK.BartuziZ. (2015). Platelet activating factor in allergies. Int. J. Immunopathol. Pharmacol.28, 584–589. 10.1177/0394632015600598
94
PalmerE. (2003). Negative selection--clearing out the bad apples from the T-cell repertoire. Nat. Rev. Immunol.3, 383–391. 10.1038/nri1085
95
ParkM-J.LeeS-Y.MoonS-J.SonH-J.LeeS-H.KimE-K.et al (2016). Metformin attenuates graft-versus-host disease via restricting mammalian target of rapamycin/signal transducer and activator of transcription 3 and promoting adenosine monophosphate-activated protein kinase-autophagy for the balance between T helper 17 and Tregs. Transl. Res.173, 115–130. 10.1016/j.trsl.2016.03.006
96
PaulW. E. (2015). History of interleukin-4. Cytokine75, 3–7. 10.1016/j.cyto.2015.01.038
97
PelletierM.MaggiL.MichelettiA.LazzeriE.TamassiaN.CostantiniC.et al (2010). Evidence for a cross-talk between human neutrophils and Th17 cells. blood115, 335–343. 10.1182/blood-2009-04-216085
98
Pérez-JeldresT.Alvarez-LobosM.Rivera-NievesJ. (2021). Targeting sphingosine-1-phosphate signaling in immune-mediated diseases: Beyond multiple sclerosis. Drugs81, 985–1002. 10.1007/s40265-021-01528-8
99
ProtoJ. D.DoranA. C.GusarovaG.YurdagulA.JrSozenE.SubramanianM.IslamM. N.et al (2018). Regulatory T cells promote macrophage efferocytosis during inflammation resolution. Immunity49, 666–677. 10.1016/j.immuni.2018.07.015
100
QuinnP. J. (2014). Sphingolipid symmetry governs membrane lipid raft structure. Biochim. Biophys. Acta1838, 1922–1930. 10.1016/j.bbamem.2014.02.021
101
RådmarkO.WerzO.SteinhilberD.SamuelssonB. (2015). 5-Lipoxygenase, a key enzyme for leukotriene biosynthesis in health and disease. Biochim. Biophys. Acta1851, 331–339. 10.1016/j.bbalip.2014.08.012
102
RajasagiN. K.RouseB. T. (2018). Application of our understanding of pathogenesis of herpetic stromal keratitis for novel therapy. Microbes Infect.20, 526–530. 10.1016/j.micinf.2017.12.014
103
RegenS. L. (2020). The origin of lipid rafts. Biochemistry59, 4617–4621. 10.1021/acs.biochem.0c00851
104
RomagnaniS. (2014). T cell subpopulations. Chem. Immunol. Allergy100, 155–164. 10.1159/000358622
105
RudenskyA. Y. (2011). Regulatory T cells and Foxp3. Immunol. Rev.241, 260–268. 10.1111/j.1600-065X.2011.01018.x
106
SofiM. H.HeinrichsJ.DanyM.NguyenH.DaiM.BastianD.et al (2017). Ceramide synthesis regulates T cell activity and GVHD development. JCI Insight2. 10.1172/jci.insight.91701
107
SakaguchiS.YamaguchiT.NomuraT.OnoM. (2008). Regulatory T cells and immune tolerance. Cell133, 775–787. 10.1016/j.cell.2008.05.009
108
SalamoneG.GiordanoM.TrevaniA. S.GamberaleR.VermeulenM.SchettinniJ.et al (2001). Promotion of neutrophil apoptosis by TNF-alpha. J. Immunol.166, 3476–3483. 10.4049/jimmunol.166.5.3476
109
SalasA.Hernandez-RochaC.DuijvesteinM.FaubionW.McGovernD.VermeireS.et al (2020). JAK-STAT pathway targeting for the treatment of inflammatory bowel disease. Nat. Rev. Gastroenterol. Hepatol.17, 323–337. 10.1038/s41575-020-0273-0
110
SallenaveJ-M.GuillotL. (2020). Innate immune signaling and proteolytic pathways in the resolution or exacerbation of SARS-CoV-2 in covid-19: Key therapeutic targets?Front. Immunol.11, 1229. 10.3389/fimmu.2020.01229
111
SandbornW. J.FeaganB. G.D'HaensG.WolfD. C.JovanovicI.HanauerS. B.et al (2021). Ozanimod as induction and maintenance therapy for ulcerative colitis. N. Engl. J. Med.385, 1280–1291. 10.1056/NEJMoa2033617
112
SchaubergerE.PeinhauptM.CazaresT.LindsleyA. W. (2016). Lipid mediators of allergic disease: Pathways, treatments, and emerging therapeutic targets. Curr. Allergy Asthma Rep.16, 48. 10.1007/s11882-016-0628-3
113
SchnoorM.AlcaideP.VoisinM-B.van BuulJ. D. (2016). Recruitment of immune cells into inflamed tissues: Consequences for endothelial barrier integrity and tissue functionality. Mediat. Inflamm.2016, 1561368. 10.1155/2016/1561368
114
SchwabS. R.CysterJ. G. (2007). Finding a way out: Lymphocyte egress from lymphoid organs. Nat. Immunol.8, 1295–1301. 10.1038/ni1545
115
SelkirkJ. V.BortolatoA.YanY. G.ChingN.HargreavesR. (2022). Competitive binding of ozanimod and other sphingosine 1-phosphate receptor modulators at receptor subtypes 1 and 5. Front. Pharmacol.13, 892097. 10.3389/fphar.2022.892097
116
SenP.AndrabiS. B. A.BuchacherT.KhanM. M.KalimU. U.LindemanT. M.et al (2021). Quantitative genome-scale metabolic modeling of human CD4+ T cell differentiation reveals subset-specific regulation of glycosphingolipid pathways. Cell Rep.37, 109973. 10.1016/j.celrep.2021.109973
117
ShibuyaM. (2011). Vascular endothelial growth factor (VEGF) and its receptor (vegfr) signaling in angiogenesis: A crucial target for anti- and pro-angiogenic therapies. Genes Cancer2, 1097–1105. 10.1177/1947601911423031
118
ShinS-H.ChoK-A.YoonH-S.KimS-Y.KimH-Y.Pewzner-JungY.et al (2021). Ceramide synthase 2 null mice are protected from ovalbumin-induced asthma with higher T cell receptor signal strength in CD4+ T cells. Int. J. Mol. Sci.22. 10.3390/ijms22052713
119
ShiowL. R.RosenD. B.BrdickováN.XuY.AnJ.LanierL. L.et al (2006). CD69 acts downstream of interferon-alpha/beta to inhibit S1P1 and lymphocyte egress from lymphoid organs. Nature440, 540–544. 10.1038/nature04606
120
SimonsK.SampaioJ. L. (2011). Membrane organization and lipid rafts. Cold Spring Harb. Perspect. Biol.3, a004697. 10.1101/cshperspect.a004697
121
SmithN. C.RiseM. L.ChristianS. L. (2019). A comparison of the innate and adaptive immune systems in cartilaginous fish, ray-finned fish, and lobe-finned fish. Front. Immunol.10, 2292. 10.3389/fimmu.2019.02292
122
Smith-GarvinJ. E.KoretzkyG. A.JordanM. S. (2009). T cell activation. Annu. Rev. Immunol.27, 591–619. 10.1146/annurev.immunol.021908.132706
123
Sobrido-CameánD.Barreiro-IglesiasA. (2018). Role of caspase-8 and Fas in cell death after spinal cord injury. Front. Mol. Neurosci.11, 101. 10.3389/fnmol.2018.00101
124
SolomouE. E.KeyvanfarK.YoungN. S. (2006). T-bet, a Th1 transcription factor, is up-regulated in T cells from patients with aplastic anemia. blood107, 3983–3991. 10.1182/blood-2005-10-4201
125
SrivastavaM.BaigM. S. (2018). NOS1 mediates AP1 nuclear translocation and inflammatory response. Biomed. Pharmacother.102, 839–847. 10.1016/j.biopha.2018.03.069
126
SteinmetzO. M.SummersS. A.GanP-Y.SempleT.HoldsworthS. R.KitchingA. R. (2011). The Th17-defining transcription factor RORγt promotes glomerulonephritis. J. Am. Soc. Nephrol.22, 472–483. 10.1681/ASN.2010040435
127
StepanovskaB.HuwilerA. (2020). Targeting the S1P receptor signaling pathways as a promising approach for treatment of autoimmune and inflammatory diseases. Pharmacol. Res.154, 104170. 10.1016/j.phrs.2019.02.009
128
SukochevaO. A.LukinaE.McGowanE.BishayeeA. (2020). Sphingolipids as mediators of inflammation and novel therapeutic target in inflammatory bowel disease. Adv. Protein Chem. Struct. Biol.120, 123–158. 10.1016/bs.apcsb.2019.11.003
129
SzaboS. J.KimS. T.CostaG. L.ZhangX.FathmanC. G.GlimcherL. H. (2000). A novel transcription factor, T-bet, directs Th1 lineage commitment. Cell100, 655–669. 10.1016/s0092-8674(00)80702-3
130
TabarkiewiczJ.PogodaK.KarczmarczykA.PozarowskiP.GiannopoulosK. (2015). The role of IL-17 and Th17 lymphocytes in autoimmune diseases. Arch. Immunol. Ther. Exp. Warsz.63, 435–449. 10.1007/s00005-015-0344-z
131
TaoS.TaoR.BuschD. H.WideraM.SchaalH.DrexlerI. (2019). Sequestration of late antigens within viral factories impairs MVA vector-induced protective memory CTL responses. Front. Immunol.10, 2850. 10.3389/fimmu.2019.02850
132
ThudichumJ. L. W. (1962). A treatise on the chemical constitution of the brain.
133
TikhonovaA. N.van LaethemF.HanadaK-i.LuJ.PobezinskyL. A.HongC.et al (2012). αβ T cell receptors that do not undergo major histocompatibility complex-specific thymic selection possess antibody-like recognition specificities. Immunity36, 79–91. 10.1016/j.immuni.2011.11.013
134
TrapaniJ. A. (2001). Granzymes: A family of lymphocyte granule serine proteases. Genome Biol.2, 3014.1–3014.7. 10.1186/gb-2001-2-12-reviews3014
135
TsukudaY.IwasakiN.SeitoN.KanayamaM.FujitaniN.ShinoharaY.et al (2012). Ganglioside GM3 has an essential role in the pathogenesis and progression of rheumatoid arthritis. PLoS One7, e40136. 10.1371/journal.pone.0040136
136
TurnerJ. E.SteinmetzO. M.StahlR. A.PanzerU. (2007). Targeting of Th1-associated chemokine receptors CXCR3 and CCR5 as therapeutic strategy for inflammatory diseases. Mini Rev. Med. Chem.7, 1089–1096. 10.2174/138955707782331768
137
UcheL. E.GoorisG. S.BouwstraJ. A.BeddoesC. M. (2021). Increased levels of short-chain ceramides modify the lipid organization and reduce the lipid barrier of skin model membranes. Langmuir37, 9478–9489. 10.1021/acs.langmuir.1c01295
138
VarelaM. L.MogildeaM.MorenoI.LopesA. (2018). Acute inflammation and metabolism. Inflammation41, 1115–1127. 10.1007/s10753-018-0739-1
139
VasandanA. B.JahnaviS.ShashankC.PrasadP.KumarA.PrasannaS. J. (2016). Human Mesenchymal stem cells program macrophage plasticity by altering their metabolic status via a PGE2-dependent mechanism. Sci. Rep.6, 38308. 10.1038/srep38308
140
ViallardJ. F.PellegrinJ. L.RanchinV.SchaeverbekeT.DehaisJ.Longy-BoursierM.et al (1999). Th1 (IL-2, interferon-gamma (IFN-g)) and Th2 (IL-10, IL-4) cytokine production by peripheral blood mononuclear cells (PBMC) from patients with systemic lupus erythematosus (SLE). Clin. Exp. Immunol.115, 189–195. 10.1046/j.1365-2249.1999.00766.x
141
VoskoboinikI.WhisstockJ. C.TrapaniJ. A. (2015). Perforin and granzymes: Function, dysfunction and human pathology. Nat. Rev. Immunol.15, 388–400. 10.1038/nri3839
142
WalshD.McCarthyJ.O'DriscollC.MelgarS. (2013). Pattern recognition receptors--molecular orchestrators of inflammation in inflammatory bowel disease. Cytokine Growth Factor Rev.24, 91–104. 10.1016/j.cytogfr.2012.09.003
143
WanY. Y. (2010). Regulatory T cells: Immune suppression and beyond. Cell Mol. Immunol.7, 204–210. 10.1038/cmi.2010.20
144
WangY.LiuJ.BurrowsP. D.WangJ-Y. (2020). B cell development and maturation. Adv. Exp. Med. Biol.1254, 1–22. 10.1007/978-981-15-3532-1_1
145
WatanabeS.YamadaY.MurakamiH. (2020). Expression of Th1/Th2 cell-related chemokine receptors on CD4+ lymphocytes under physiological conditions. Int. J. Lab. Hematol.42, 68–76. 10.1111/ijlh.13141
146
WeiratherJ.HofmannU. D. W.BeyersdorfN.RamosG. C.VogelB.FreyA.et al (2014). Foxp3+ CD4+ T cells improve healing after myocardial infarction by modulating monocyte/macrophage differentiation. Circ. Res.115, 55–67. 10.1161/CIRCRESAHA.115.303895
147
WieseT.DennstädtF.HollmannC.StonawskiS.WurstC.FinkJ.et al (2021). Inhibition of acid sphingomyelinase increases regulatory T cells in humans. Brain Commun.3, fcab020. 10.1093/braincomms/fcab020
148
XieS. Z.Garcia-PratL.VoisinV.FerrariR.GanO. I.WagenblastE.et al (2019). Sphingolipid modulation activates proteostasis programs to govern human hematopoietic stem cell self-renewal. Cell Stem Cell25, 639–653. e7. 10.1016/j.stem.2019.09.008
149
YamadaA.ArakakiR.SaitoM.KudoY.IshimaruN. (2017). Dual role of fas/FasL-mediated signal in peripheral immune tolerance. Front. Immunol.8, 403. 10.3389/fimmu.2017.00403
150
YamanakaK.TakahashiY.AzumaY.HantaniY. (2020). Assay development and screening for the identification of ganglioside GM3 synthase inhibitors. Biochemistry59, 1242–1251. 10.1021/acs.biochem.0c00055
151
YanaiH.ChibaS.HangaiS.KometaniK.InoueA.KimuraY.et al (2018). Revisiting the role of IRF3 in inflammation and immunity by conditional and specifically targeted gene ablation in mice. Proc. Natl. Acad. Sci. U. S. A.115, 5253–5258. 10.1073/pnas.1803936115
152
YangS.FujikadoN.KolodinD.BenoistC.MathisD. (2015). Immune toleranceRegulatory T cells generated early in life play a distinct role in maintaining self-tolerance. Science348, 589–594. 10.1126/science.aaa7017
153
YatimK. M.LakkisF. G. (2015). A brief journey through the immune system. Clin. J. Am. Soc. Nephrol.10, 1274–1281. 10.2215/CJN.10031014
154
YeJ. (2013). Roles of regulated intramembrane proteolysis in virus infection and antiviral immunity. Biochim. Biophys. Acta1828, 2926–2932. 10.1016/j.bbamem.2013.05.005
155
YoshieO.MatsushimaK. (2015). CCR4 and its ligands: From bench to bedside. Int. Immunol.27, 11–20. 10.1093/intimm/dxu079
156
ZhangL.LiuM.LiuW.HuC.LiH.DengJ.et al (2021). Th17/IL-17 induces endothelial cell senescence via activation of NF-κB/p53/Rb signaling pathway. Lab. Invest.101, 1418–1426. 10.1038/s41374-021-00629-y
157
ZhaoX.ZhaoY.SunX.XingY.WangX.YangQ. (2020). Immunomodulation of MSCs and MSC-derived extracellular vesicles in osteoarthritis. Front. Bioeng. Biotechnol.8, 575057. 10.3389/fbioe.2020.575057
158
ZhuY.GumlawN.KarmanJ.ZhaoH.ZhangJ.JiangJ-L.et al (2011). Lowering glycosphingolipid levels in CD4+ T cells attenuates T cell receptor signaling, cytokine production, and differentiation to the Th17 lineage. J. Biol. Chem.286, 14787–14794. 10.1074/jbc.M111.218610
159
ZindelJ.KubesP. (2020). DAMPs, PAMPs, and LAMPs in immunity and sterile inflammation. Annu. Rev. Pathol.15, 493–518. 10.1146/annurev-pathmechdis-012419-032847
160
ZingoniA.SotoH.HedrickJ. A.StoppacciaroA.StorlazziC. T.SinigagliaF.et al (1998). Preferentially expressed in Th2 but not Th1 cutting edge: The chemokine receptor CCR8 is cells. J. Immunol.161, 547–551.
161
ZumerleS.MolonB.ViolaA. (2017). Membrane rafts in T cell activation: A spotlight on CD28 costimulation. Front. Immunol.8, 1467. 10.3389/fimmu.2017.01467
162
ZuoW.ChenY-G. (2009). Specific activation of mitogen-activated protein kinase by transforming growth Factor- receptors in lipid rafts is required for epithelial cell plasticity. Mol. Biol. Cell20, 1020–1029. 10.1091/mbc.e08-09-0898
Summary
Keywords
ceramides, gangliosides, S1P, lipid rafts, treg, Th17, bioactive lipids
Citation
Hartel JC, Merz N and Grösch S (2022) How sphingolipids affect T cells in the resolution of inflammation. Front. Pharmacol. 13:1002915. doi: 10.3389/fphar.2022.1002915
Received
25 July 2022
Accepted
24 August 2022
Published
13 September 2022
Volume
13 - 2022
Edited by
Paola Patrignani, University of Studies G. d'Annunzio Chieti and Pescara, Italy
Reviewed by
Stefania Tacconelli, University of Studies G. d'Annunzio Chieti and Pescara, Italy
Annalisa Contursi, University of Studies G. d'Annunzio Chieti and Pescara, Italy
Updates
Copyright
© 2022 Hartel, Merz and Grösch.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Sabine Grösch, groesch@em-uni-frankfurt.de
This article was submitted to Inflammation Pharmacology, a section of the journal Frontiers in Pharmacology
Disclaimer
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.