REVIEW article

Front. Pharmacol., 12 July 2022

Sec. Ethnopharmacology

Volume 13 - 2022 | https://doi.org/10.3389/fphar.2022.920823

Dendrobium huoshanense C.Z.Tang et S.J.Cheng: A Review of Its Traditional Uses, Phytochemistry, and Pharmacology

    LG

    Leilei Gao 1,2*

    FW

    Fang Wang 1,2

    TH

    Tingting Hou 1,3

    CG

    Chunye Geng 1,3

    TX

    Tao Xu 1,2

    BH

    Bangxing Han 1,2*

    DL

    Dong Liu 1,2*

  • 1. College of Biological and Pharmaceutical Engineering, West Anhui University, Lu’an, China

  • 2. Anhui Province Traditional Chinese Medicine Resource Protection and Sustainable Utilization Engineering Laboratory, Lu’an, China

  • 3. School of Pharmacy, Anhui University of Chinese Medicine, Hefei, China

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Abstract

Dendrobium huoshanense, a traditional medicinal and food homologous plant, belongs to the family Orchidaceae and has a long history of medicinal use. It is reported that the stem of D. huoshanense has a variety of bioactive ingredients such as polysaccharides, flavonoids, sesquiterpenes, phenols, etc. These bioactive ingredients make D. huoshanense remarkable for its pharmacological effects on anti-tumor, immunomodulation, hepatoprotective, antioxidant, and anticataract activities. In recent years, its rich pharmacological activities have attracted extensive attention. However, there is no systematic review focusing on the chemical compositions and pharmacological effects of D. huoshanense. Therefore, the present review aims to summarize current research on the chemical compositions and pharmacological activities of D. huoshanense. This study provides valuable references and promising ideas for further investigations of D. huoshanense.

Introduction

Dendrobium is a valuable traditional Chinese medicine with a long history of medicinal use (Li et al., 2022; Zhu et al., 2021; Niu et al., 2018). It was first recorded in “Shennong’s Herbal Classic” (Chen et al., 2021). However, there are various species in this genus, and their quality varies significantly (Chen et al., 2011). Dendrobium huoshanense C.Z.Tang et S.J.Cheng is an endemic epiphytic orchid species as well as a national geographical indication product of China (Figure 1) (Huang et al., 2007). This plant was first described in a local classic reference “Bai Cao Jing” (《百草镜》) and the distribution area of this plant is in the Da-bie Mountains and adjacent areas, especially in the Huoshan County town, Anhui province, China (Zhao et al., 2017; Yuan et al., 2019; Chen et al., 2020). Currently, the species has been included in the Pharmacopoeia of the Peoples Republic of China (2020 Edition) and approved to be used as food (Hao et al., 2018; Editorial Board of China Pharmacopoeia Committee 2020; Hao et al., 2021c).

FIGURE 1

FIGURE 1

The whole plant (A), fresh strips (B), and the commercial product named Fengdou(C) of D. huoshanense.

D. huoshanense is locally known as “Mihu,” and its stem has high medicinal value, which is sweet in taste and slightly cold in nature. It is commonly utilized for benefiting the stomach and producing body fluid, clearing heat, and nourishing yin (Hsieh et al., 2008; Wang et al., 2009; Dai et al., 2021). Previous studies have showed that D. huoshanense has various activities, such as immunoregulation, anti-oxidation, anti-cataract, anti-glycation, anti-aging, anti-cataract, antitumor, anti-rheumatoid arthritis, anti-atherosclerosis, anti-inflammation, hypoglycemic activity, and liver protection activities (Wu et al., 2009; Ohara et al., 2013; Deng et al., 2016; Zhang et al., 2020; Hao J.-W. et al., 2021; Zhu et al., 2022). The main components that play a therapeutic role are active substances such as flavonoids, sesquiterpenes, and especially polysaccharides, which is the index of the quality evaluation of D. huoshanense (Lee and Chen 2014; Liu et al., 2018; Yuan et al., 2018; Chen et al., 2019; Zhou et al., 2020; Hao et al., 2021a; Wang et al., 2021). These diverse chemical compositions and extensive pharmacological activities of D. huoshanense have attracted much attention, while posing great difficulties for further research.

At present, there is much literature summarizing the Dendrobium genus, but no literature systematically reviewing the status of the research on D. huoshanense. Therefore, this review systematically describes the current research status of a specific species, D. huoshanense, including the materia medica research, resource, phytochemistry, and pharmacology, to provide a reference for further research on D. huoshanense.

Materia Medica Research

Materia Medica Research on Origin

The habitats of D. huoshanense have been recorded in many important classical documents of traditional Chinese medicine. In the period of 220–450 AD, “Records of Famous Doctors” (《名医别录》) recorded that “Dendrobium grows on the stone beside the water in the valley of Lu’an”. It is found that Lu’an refers to the area of Huoshan in the Anhui province at that time, demonstrating that the earliest recorded origin of D. huoshanense is in the Huoshan County. “Bai Cao Jing” (《百草镜》) in the Qing Dynasty described that Dendrobium from the Lu’an and Huoshan Counties was called “Huoshan Shihu” and was the best, which was first documented with the name of D. huoshanense. In 1984, Tang et al. systematically studied several species of Dendrobium and analyzed their botanical traits (Tang and Cheng 1984). Moreover, “Mihu” produced in the Huoshan County was officially named Dendrobium huoshanense C.Z. Tang et S.J. Cheng.

At present, “Flora Reipublicae Popularis Sinicae” reported that “D. huoshanense is produced in southwestern Henan (Nanzhao) and southwestern Anhui (Huoshan) of China. It grows on tree trunks in mountain forests and on rocks in valleys. The type specimen was collected in Anhui (Huoshan) of China”. Through materia medica research and field investigations, Liu and wang et al. also clearly support the occurrence of D. huoshanense in Huoshan, Anhui province of China (Liu 1996; Wang and Peng 2004). In 2007, D. huoshanense was listed in the national geographic indication to protect products. In short, the habitats of D. huoshanense are mainly concentrated in Huoshan, Anhui province of China.

Materia Medica Research on Medicinal Use

D. huoshanense has been used in medicine for a long time. “Records of Famous Doctors” (《名医别录》) recorded that Dendrobium could nourish the essence, reinforce the kidneys, calm the stomach, build muscles, relieve foot and knee pain, and remove convulsions, while emphasizing that the origin of Dendrobium is the now Huoshan County. These are enough to show that the orchid plant recorded in “Records of Famous Doctors” (《名医别录》) refers to D. huoshanense (Tao 2013). The ancient medical reference “Bai Cao Jing” (《百草镜》) put forward the local name of D. huoshanense for the first time and stated that this plant is the best for medicinal use. The traits of D. huoshanense, including dwarf plantlets in a cluster and an upright or bending stem resembling grasshopper legs, are almost identical to those recorded in the “Shen Nong Ben Cao” (Zhao 1998). It can be seen that D. huoshanense has been used as high-quality Dendrobium for medicinal purposes for many generations. In addition, D. huoshanense is included in the Chinese Pharmacopoeia (2020 version), and its efficacy is recorded for benefiting the stomach and producing body fluids, clearing heat, and nourishing the yin (Editorial Board of China Pharmacopoeia Committee 2020).

Resource

Resource Distribution

Dendrobium is the second largest genus belonging to the family Orchidaceae, widely distributed in Asia, Europe, Oceania, and other regions (Liu JL. et al., 2020; Han et al., 2020; Hu et al., 2020; Wang et al., 2020). There are 105 species and two varieties of Dendrobium in China, which are produced in Anhui, Zhejiang, Jiangxi, Fujian, Taiwan, Hubei, Hunan, Guangdong, Guangxi, Hainan, Sichuan (including Chongqing), Guizhou, Yunnan, Tibet, etc. Among them, there are 39 species of Dendrobium with medicinal purposes, including D. huoshanense (Chiang et al., 2012; Ye et al., 2021). However, the natural distribution of D. huoshanense is relatively narrow, because of its preference for a cool and moist environment, and high environmental requirements (Liu et al., 2007; Qin et al., 2008; Niu et al., 2020). Wild D. huoshanense mainly grows as an epiphytic on the cliffs beside rivers and valleys at a slight altitude ranging between 200 and 1,200 m. In view of its strict requirements on the environment, D. huoshanense is currently endemic to the Da-bie Mountains, with Huoshan in the Anhui Province as the center of distribution (Zha et al., 2007d; Zheng et al., 2011; Wang et al., 2012; Wu et al., 2016). The cultivation industry of D. huoshanense is also in Huoshan, which has reached 8.0 million m2 with 350 tons of the annual production (including flower, fresh and dry materials of D. huoshanense).

Resource Conservation

Over the years, due to the unreasonable collection of D. huoshanense and the limitation of its own reproduction modes, the wild resources are on the verge of extinction. It has been listed in the National Key Protected Wild Plants as a “Class I protected species” (http://www.gov.cn/zhengce/zhengceku/2021-09/09/content_5636409.htm). In order to improve resource conservation and resolve the market demand, key measures must be taken. By mastering the distribution, reserves, and the native environment of D. huoshanense, the provenance protection base has been established and some local standards such as “Dendrobium huoshanense C. Z. Tang et S. J. Cheng” and “Technical regulation for the protection of the protospecies of Dendrobium huoshanense C. Z. Tang et S. J. Cheng” have been promulgated. In addition, the rapid breeding of D. huoshanense has been realized through modern biotechnology, which effectively alleviates the resource situation of D. huoshanense (Luo et al., 2003; Shi et al., 2003).

Scientific-based harvesting is also an important factor for resource protection and reserve. The suitable harvesting time for D. huoshanense is from November to June of the following year, and there are two ways for harvesting: “Cai Lao Liu Xin” and whole plant harvesting (Ren et al., 2014). “Cai Lao Liu Xin” is the method of harvesting certain stems with ages of more than 3 years old, while whole plants should be harvested from clumps of more than 20 months old. Furthermore, the cultivation modes of D. huoshanense have been systematically researched, including facility cultivation mode, under-forest cultivation mode, and simulative habitat cultivation mode, and provide powerful help for the conservation resources of D. huoshanense (Figure 2) (Yi et al., 2021). All in all, the resources of D. huoshanense have been effectively protected and rationally exploited.

FIGURE 2

FIGURE 2

External (A1–A3) and internal (B1–B3) growth environments of D. huoshanense under the facility cultivation mode, under-forest cultivation mode, and simulative habitat cultivation mode, respectively.

Constituents From D. huoshanense

Modern biomedical researches show that D. huoshanense contains many active ingredients, such as polysaccharides, flavonoids, bibenzyls, phenols, sesquiterpenoids, etc. It is confirmed that these active ingredients are used singly or in combinations to make D. huoshanense of high medicinal value. The following sections will elaborate on the bioactivity present in between the ingredients and D. huoshanense.

Polysaccharides

Polysaccharide is the main active ingredient of D. huoshanense, which is effective in anti-inflammation, anti-oxidant, anti-tumor, and immunoregulation, and is mainly composed of glucose, galactose, xylose, and arabinose and mannose, with the molecular weight distribution range of 3,200–8,090,000 Da (Zha et al., 2007b; Xie et al., 2018; Hao et al., 2019; Dai et al., 2020; Yi et al., 2021b; Zhang Y. et al., 2021; Wu et al., 2022). At present, a variety of D. huoshanense polysaccharides have been isolated, such as DHAH (Meng et al., 2013), DHP (Pan et al., 2014), DHP1A (Tian et al., 2013), HPS-1B23 (Zha et al., 2007c), DHP-W2 (Pan et al., 2013), DHPD1 (Zha et al., 2013), DHPD2 (Li et al., 2014), TC-DHPA4 (Si et al., 2018), GXG (Xie et al., 2019a), cDHPS (Liu B. et al., 2020; Shang et al., 2021), and DHP-4A (Li et al., 2015). Their structures are identified by GC-MS, HPLC-GPC, IR, NMR, HSQC, and HMBC, and the details are shown in Table 1.

TABLE 1

Compound nameMonosaccharide compositionStructural featuresMolecular weight (Da)References
DHAHMan: Glc: Gal = 100:53:02ND2.2×105Meng et al. (2013)
DHPAra: Gal: Glc: Man = 0.03:0.11:1.00:0.07NDNDPan et al. (2014)
DHP1AMan: Glc: Gal = 2.5:16:1(1→4)-linked α-D-Glcp, (1→6)-linked α-D-Glcp and (1→4)-linked β-D-Manp6.7×103Tian et al. (2013)
HPS-1B23Glc: Man: Gal = 31:10:8(1→4)-linked α-D-Glcp, (1→6)-linked α-D-Glcp, (1→3,6)-linked α-D-Glcp and (1→3,6)-linked α-D-Manp2.2×104Zha et al. (2007c)
DHP-W2Glc: Xyl: Gal = 1.0:1.0:0.5(1→6)-linked β-D-Glcp, (1→4)-linked β-D-Glcp and (1→4,6)-linked β-D-Glcp with branches at O-4/67.3×104Pan et al. (2013)
DHPD1Glc: Ara: Gal = 0.023:1.023:0.021ND3.2×103Zha et al. (2013)
DHPD2Gala: Glc: Ara = 0.896:0.723:0.2(1→5)-linked α-L-Araf, (1→6)-linked α-D-Glcp, (1→6)-linked β-D-Glcp, (1→4)-linked β-D-Glcp, (1→3,6)-linked β-D-Galp and (1→6)-linked β-D-Galp, with the branches of terminal α-D-Xlyp and β-D-Manp.8.09×106Li et al. (2014)
TC-DHPA4Rha: Ara: Man: Glc: Gala = 1.28:1:1.67:4.71:10.43(1→6)-linked β-Galp, (1→4)-linked β-Glcp, and (1→6)-linked β-Glcp, with four branched chains8.0 × 105Si et al. (2018)
GXGGlc: Xyl: Gal = 2.85 : 2.13 : 1.00(1→4)-linked Xylp, (1→2,4)-linked Xylp, (1→4)-linked Galp, (1→3,6)-linked Galp, (1→6)-linked Glcp, (1→4)-linked Glcp and (1→4,6)-linked Glcp1.78×106Xie et al. (2019a)
CdhpsMan: Glc = 2.88:1.00β-(1→4)-linked D-Glcp and β-(1→4)-linked D-Manp with partial acetylation at 3-OH2.59×105(Liu et al., 2020a; Shang et al., 2021)
DHP-4AGlc:Ara: Man: Rha = 13.8:3.0:6.1:2.1(1→6)-linkedβ-D-Glcp, (1→6)-linkedβ-D-Manp and (1→3,6)-linked β-D-Manp, with branches at the C-3 position of (1→6)-linkedβ-D-Manp2.32×105Li et al. (2015)

Summary of polysaccharides from D. huoshanense.

Ara, arabinose; Man, mannose; Glc, glucose; Gal, galactose; Xyl, xylose; Rha, rhamnose.

Sesquiterpenoids

Sesquiterpenoids are essential chemical components, which are also the significant material basis for the pharmacological activity of Dendrobium (Fan et al., 2021). Sesquiterpenoids (1–5) have also been found in D. huoshanense and their structures have been elucidated through extensive spectroscopic analyses. The detailed structural information is shown in Figure 3 (Chen et al., 2022).

FIGURE 3

FIGURE 3

Structure of sesquiterpenes (1–5) in D. huoshanense.

Flavonoids

Flavonoids, which are the most widely distributed class of compounds in Chinese herbal medicine, are important chemical constituents of Dendrobium (Yuan et al., 2022). Flavonoids are also found in D. huoshanense, but only few flavonoids and flavonoid glycosides (6–11) have been isolated, and their structures are shown in Figure 4 (Chang et al., 2010; Zhao et al., 2021).

FIGURE 4

FIGURE 4

Structure of flavonoids (6–11) in D. huoshanense.

Bibenzyls

Bibenzyls, with a basic structural skeleton 1, 2-diphenylethane, are widely distributed in Dendrobium (Sun et al., 2021). Currently, few bibenzylates have been isolated from D. huoshanense. According to the literature statistics, eight bibenzylates (12–19) have been isolated and identified from D. huoshanense (Figure 5) (Li QM. et al., 2020; Zhao et al., 2021).

FIGURE 5

FIGURE 5

Structure of bibenzyls (12–19) in D. huoshanense.

Phenols

Phenols are widely present in Dendrobium and have complex and diverse structures, but are not the main active components (Dong et al., 2020). D. huoshanense also contains a large number of phenols, and 20 phenols have been isolated. Their chemical structures are shown in Figure 6 (Chang et al., 2010; Zhao et al., 2021).

FIGURE 6

FIGURE 6

Structure of phenols (20–39) in D. huoshanense.

Other Compounds

In addition to the aforementioned chemical constituents, D. huoshanense also contains other types of chemical constituents (Chang et al., 2010; Zhao et al., 2021), including Malic acid, Dimethyl malate, N-phenylacetamide, Isopentyl butyrate, Shikimic acid, etc., and their chemical structures are shown in Figure 7.

FIGURE 7

FIGURE 7

Structure of other compounds (40–54) in D. huoshanense.

Pharmacology

The active components in D. huoshanense are diverse and complex, and have many important effects. The pharmacological effects and possible mechanisms of action of D. huoshanense will be elaborated and reviewed below.

Antitumor Activity

As a special species of the Dendrobium genus, D. huoshanense has the ability to improve body function to prevent and treat tumor diseases. In the cell proliferation assay, the aqueous extract of D. huoshanense could effectively inhibit the growth of HeLa cells with a concentration range of 2–10 mg/ml (Zhang X. et al., 2021). Moreover, the inhibition rates of the aqueous extract of different plant ages on HeLa cells were varied. Among them, the aqueous extract of a 3 year old D. huoshanense possessed the highest anticancer activity, followed by a 2 year old, and the annuals were the least active.

In addition to the crude extract of D. huoshanense, polysaccharides and small molecule compounds isolated from D. huoshanense also have good antitumor activities. Luo et al. investigated the inhibitory effect of D. huoshanense polysaccharide cDHPS on gastric cancer and preliminarily explored its constitutive relationship. The results showed that cDHPS (at 0.2 mg/ml) could significantly inhibit tumor growth, induce tumor cell apoptosis, suppress tumor angiogenesis, and enhance a T cell immune response of murine forestomach carcinoma tumor-bearing mice (Liu B. et al., 2020; Liu et al., 2021). The structure–activity relationship investigation indicated that the molecular weight and the O-acetyl group of D. huoshanense polysaccharides greatly influenced the anti-gastric cancer activity.

Xu et al. isolated five picrotoxane-type sesquiterpenes (1–5) from D. huoshanense and examined their cytotoxicity activity on HL-60, MCF-7, SMMC-7721, and SW-480 human cancer cells (Chen et al., 2022). The results showed that compound 3 (Figure 3) showed significant effects on HL-60, MCF-7, SMMC-7721, and SW-480 cells with IC50s of 5.81, 6.49, 9.65, and 6.80 μM, respectively. Importantly, the cytotoxicity activity of compound 3 was comparable to 5-fluorouracil. To sum up, sesquiterpenes from D. huoshanense were worth further studying to find novel anticancer drugs.

Antioxidant Activity

Many traditional Chinese medicines have been reported to possess antioxidant activity (Muhammad et al., 2022). Compared with synthetic antioxidants, herbal components have the advantage of less toxicity, so they have attracted much attention. D. huoshanense, an important traditional Chinese medicine, has also shown a significant antioxidant activity. Luo et al. compared the antioxidant activity of crude polysaccharides of D. huoshanense with those crude polysaccharides of D. officinale, D. nobile, and D. chrysotoxum (Pan et al., 2014). The results showed that D. huoshanense polysaccharide had the strongest antioxidant activity and could significantly enhance the activities of antioxidative enzymes superoxidedismutase (SOD) and catalase (CAT) and increase the content of L-glutathione (GSH). In addition, refined D. huoshanense polysaccharides were isolated to investigate the antioxidant activity (Xu et al., 2019). It was found that a refined D. huoshanense polysaccharide could reduce malonaldehyde (MDA) levels, increase T-AOC levels, alleviate D-galactose-induced oxidative damage in mice, and exhibit a significant antioxidant activity.

Luo et al. have also proved that DHP1A obtained from D. huoshanense possesses a remarkable inhibition effect on the lipid peroxidation in vitro (Tian et al., 2013). At a concentration of 2.0 mg/ml, the inhibition rate of DHP1A reached 56.5%, which was higher than that of dextran (p < 0.01) and close to that of vitamin C. Furthermore, DHP1A could alleviate the hepatic oxidative stress caused by CCl4 in mice. These findings demonstrated that DHP1A exhibited a significant antioxidant activity and its mechanism of action might be anti-lipid peroxidation.

Immunomodulatory Activity

Immune function is the body’s resistance to diseases, improving the immunity of the body and enhancing the immune function is the key to prevent the occurrence of diseases and restore health. D. huoshanense has an excellent performance in immune regulation. For example, Wong et al. demonstrated that the administration of a crude polysaccharide of D. huoshanense (DH-PS) in mice not only induced the production of cytokines (IL-12 p40, IL-6, IL-10, and TNF-α) and chemokines (KC, RANTES, MCP-1, and MIP-1β), but also activated or amplified various immune cells, including NK cells/activated NK cells, NKT cells/activated NKT cells, regulatory T cells, B cells/activated B cells, CD4+ T cells/activated CD4+ T cells, and CD8+ T cells/activated CD8+ T cells (Lin et al., 2014). These results indicate that DH-PS regulated the immune function through stimulating cytokine secretions and promoting the expansions and/or activations of immune cells.

Luo et al. have investigated the immune activity of polysaccharides from D. huoshanense at different growth stages (Chen et al., 2012). The results showed that the polysaccharides obtained from different growth stages of D. huoshanense could promote the production of interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) in mouse spleen cells, showing similar immune activities. Later, some authors confirmed that the water-soluble polysaccharides HPS-1B23, HPS1A, HPS1B, and HPS2B isolated from D. huoshanense exhibited immunomodulatory activities, through stimulating macrophages to secrete IFN-γ and TNF-α (Zha, Luo and Jiang 2007a; Zha et al., 2007c). Further study revealed that HPS2B23 activated macrophages by binding to toll-like receptor 4 and triggering nuclear factor-kappa B (NF-κB), mitogen-activated protein kinases (MAPKs), and phosphoinositide 3-kinase-Akt (PI3 K/Akt) signaling pathways (Xie et al., 2016). In addition, HPS-1B23 has also been shown to modulate the small intestinal immune system and the systemic immune system after oral administration (Zha et al., 2014). Luo et al. also fractionated the homogeneous polysaccharides DHP-4A and GXG from D. huoshanense, which showed significant immunomodulatory effects (Li et al., 2015; Xie et al., 2019b). In general, D. huoshanense polysaccharides have a significant activity in immunomodulation but the structure–activity relationship has not been systematically studied.

Protective Effects on Liver

The liver, which is the largest digestive and metabolic organ in the body, can metabolize endogenous or exogenous toxic substances and is highly susceptible to damage by various toxic substances. Therefore, it is especially necessary to improve the protection of the liver. In previous studies, our research team investigated the protective effect of D. huoshanense on liver injuries in mice. First, we compared the effect of D. huoshanense with the other four species of Dendrobium (D. officinale (Huoshan), D. officinale (Yunnan), D. moniliforme, and D. henanense) (Wang et al., 2017). It was found that the administration of freshly squeezed juices of Dendrobium at the dose of 1.25 and 7.5 g‧kg−1 for 2 weeks had a protective effect on CCl4-induced acute liver injury, but the effect was different, among which, D. huoshanense had the significant activity compared with others. Thereafter, the hepatoprotective effects of D. huoshanense with different cultivation patterns and growth years were also investigated. The results showed that D. huoshanense cultivated in under-forest cultivation planting had better protective effects on acute liver injuries induced by carbon tetrachloride, acetaminophen, and cyclophosphamide in mice than D. huoshanense cultivated in greenhouse (Li Z. Q. et al., 2020). In addition, D. huoshanense plants of different ages could reduce the acute liver injury induced by acetaminophen in mice, where the 2-year old D. huoshanense had the best efficacy (Li et al., 2021).

Luo et al. investigated that the protective effects of crude polysaccharides from D. huoshanense on CCl4-induced acute liver injuries in mice (Huang et al., 2013). The results showed that the crude polysaccharides with different doses (200, 100, and 50 mg‧kg−1) could reduce the activity of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the serum and the level of hepatic MDA, enhance the activity of hepatic SOD, inhibit the expression of TNF-α in hepatocytes, and alleviate liver tissue damage induced by CCl4, indicating that the D. huoshanense polysaccharide could protect mice from CCl4-induced acute liver injury by scavenging free radicals and inhibiting lipid peroxidation and the expression of TNF-α. Furthermore, Luo et al. further found that refined polysaccharides, galactoglucomannan form D. huoshanense, dose-dependently inhibited the activity of ALT, AST, and lactate dehydrogenase (LDH). At a dose of 200 mg‧kg−1‧day−1, galactoglucomannan significantly decreased the selenite-increased activities of ALT, AST, and LDH, and reduced the MDA levels and H2O2 to 59.5% and 34.6%, respectively (Pan et al., 2012). In addition, galactoglucomannan could reverse the selenium-induced decrease of the concentration of GSH in the liver and inhibit selenium-induced transforming growth factor β1 (TGF-β1) expression, showing significant potential to prevent liver injury and fibrosis. Interestingly, the daily supplementation of galactoglucomannan prevents ethanol-induced liver injury. The results of the proteomic analysis and metabolomic analysis indicated that galactoglucomannan might correct the perturbed metabolism pathways by ethanol exposure to protect the liver from ethanol-induced injuries (Wang et al., 2014; Wang et al., 2015).

DHP1A, a polysaccharide isolated from D. huoshanense, could also reduce the levels of ALT, AST, LDH, and 8-hydroxy-20-deoxyguanosine (8-OhdG) in the serum, exhibiting a strong hepatoprotective activity. Meanwhile, DHP1A also down-regulated the expression of TNF-α, interleukin-1β (IL-1β), monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory protein-2 (MIP-2), CD68, and phosphorylated IκBα (p-IκBα), demonstrating that the hepatoprotective activity of DHP1A was related with its anti-inflammatory activity (Tian et al., 2015).

Anticataract Activity

D. huoshanense is an important traditional Chinese medicine with a protective effect on the eyes and has long been used to prepare eye protection preparations, such as Shihu Yeguang pills (Editorial Board of China Pharmacopoeia Committee 2020; Horng et al., 2021). Therefore, D. huoshanense is of interest for the treatment of eye diseases. Luo et al. found that crude polysaccharides from D. huoshanense could inhibit the oxidation pathway by the down-regulation of inducible nitric oxide synthase (iNOS) gene expression and advanced glycation end product (AGEs) formations to suppress diabetic cataract, which is an important cause of blindness worldwide (Luo et al., 2008). Especially, at 200 mg.kg−1.day−1, the crude polysaccharide significantly reduced the level of nitric oxide (NO) (70.12 ± 1.2 μmol mg−1 protein) and the activity of iNOS (U mg−1 protein), and the fluorescence intensity of AGEs was remarkably inhibited, which showed that the D. huoshanense polysaccharide has potential for the prevention and cure of diabetic cataract. Another study showed that the D. huoshanense polysaccharide significantly improved the level of GSH, decreased the content of MDA, and increased the activities of glutathione peroxidase (GSH-PX), glutathione reductase (GR), glutathione S-transferase (GST), SOD, and CAT in the lens of diabetic rats, indicating that the D. huoshanense polysaccharide may also prevent the development of diabetic cataracts by ameliorating oxidative stress (Li et al., 2012). In order to investigate the core structure of the D. huoshanense polysaccharide against cataracts, the refined polysaccharide DHPD1 was enzymatically hydrolyzed with pectinase to obtain different fragments of oligosaccharides and their anti-cataract activities were evaluated with the apoptosis model of human lens epithelial cells induced by H2O2 (Zha et al., 2017). The results showed that DHPD1-24, composed of (1 → 5)-linked-Araf, (1 → 3,6)-linked-Manp, 1-linked-Glcp, (1 → 4)-linked-Glcp, (1 → 6)-linked-Glcp, (1 → 4,6)-linked-Glcp, (1 → 6)-linked-Galp, and 1-linked-Xylp in a molar ratio of 1.06:1.53:2.11:2.04:0.93:0.91:0.36:1.01, was the core structure of the D. huoshanense polysaccharide against cataract, and it could inhibit H2O2-induced human lens epithelial cell apoptosis through suppressing the MAPKs signaling pathway, which provided the foundation for further unraveling the structure–activity relationship of the D. huoshanense polysaccharide against cataract.

Antiglycation Activity

Glycation is a non-enzymatic reaction that inserts sugar chains into macromolecules such as proteins, DNA, and lipids, to form stable covalent structures. These bound products are involved in developing diabetes, metabolic syndrome, obesity, hypertension, atherosclerosis, and Alzheimer’s disease to result in body damage. Studies have shown that D. huoshanense polysaccharides have significant anti-glycation effects. For example, a polysaccharide, DHP-W2, isolated from D. huoshanense achieved 23% inhibition of protein glycation after 3 weeks of reaction at a concentration of 0.5 mg/ml, which was similar to that of vitamin C at 0.3 mg/ml (Inhibition of protein glycosylation, 28%) (Pan et al., 2013). Luo et al. isolated DHPD1 from D. huoshanense and prepared DHPD1 derivatives under enzymatic degradation conditions to investigate the inhibitory effects of molecular weight alteration of the D. huoshanense polysaccharide on protein glycation (Zha et al., 2013). The results indicated that the anti-glycation activity of the D. huoshanense polysaccharide reduced with the decrease of the molecular weight.

Furthermore, the sulfated DHPD1 with a degree of substitution of 1.473 was studied, displaying that the inhibition activity of sulfated DHPD1 on protein glycation at 1.0 mg/ml was 58.5%, which was 16.2% and 52.5% higher than the same dose of aminoguanidine and DHPD1, respectively (Qian et al., 2014). Luo et al. also extracted DHPD2 from D. huoshanense by fractionation on the DEAE-Cellulose column and dialysis (molecular weight cut off: 8,000 Da), and simultaneously prepared sulfated DHPD2 derivatives by chlorosulfonic acid-pyridine (CSA-Pyr) method (Li et al., 2014). The results of the anti-glycation assay showed that the activity of DHPD2 was enhanced after sulfation and was more favorable at C-2 and C-6 sulfations of the glycosyl residues.

Others

In addition to the bioactivities described previously, it was found that D. huoshanense also had important effects on anti-aging (Gu F. L. et al., 2021), anti-rheumatoid arthritis (Shang et al., 2021), anti-atherosclerosis (Fan et al., 2020), anti-inflammation (Ge et al., 2018; Gu FL. et al., 2021), hypoglycemic activity (Pan et al., 2014; Wang et al., 2019), regulation of intestinal flora (Xie et al., 2019a), and constipation caused by the spleen’s yin deficiency (Gan et al., 2019). However, its specific mechanism of action is still to be further explored. All in all, D. huoshanense is worthy for an in-depth study.

Conclusion and Future Prospects

As the best of

Dendrobium

,

D. huoshanense

has attracted increasing attention. Currently, the materia medica research and resource aspects of

D. huoshanense

have been evident. Furthermore, the

D. huoshanense

industry is vast, with an 8.0 million m

2

promoted planting area and 350 tons of the annual production (including flower, fresh and dry materials of

D. huoshanense

) in Lu’an of the Anhui province. Therefore, this review systematically summarized the recent research on the chemical composition and pharmacological effects of

D. huoshanense

to provide references for further research on

D. huoshanense

, as well as help in a more in-depth understanding, development, and utilization of

D. huoshanense

. Importantly, this review reveals that many aspects of

D. huoshanense

warrant further investigation. The detailed discussion is as follows.

  • (i) Although efficient methods for the conservation resources of D. huoshanense have been obtained, further studies aiming at improving bioactive secondary metabolites in cultivated D. huoshanense should be actively performed, which are relevant to its pharmacological activity and beneficial for its commercialization.

  • (ii) Via different isolation methods, D. huoshanense polysaccharides with different structures can be obtained so that D. huoshanense polysaccharides are worthy of continued research.

  • (iii) Compared with other natural polysaccharides, the pharmacological effects of D. huoshanense polysaccharides are still under-researched, which need to be comprehensively studied to improve the medicinal value of D. huoshanense.

  • (iv) There are many studies on immunomodulation of the D. huoshanense polysaccharide, which shows desirable results, but its druggability has not been studied in depth.

  • (v) Compared with D. officinale, there are fewer studies on the small molecule chemical compositions of D. huoshanense, and there remains significant room for further research.

  • (vi) Many studies on the pharmacological activity and chemical compositions of D. huoshanense have not clearly marked the origin, including the cultivation mode and growth years, which is unfavorable to the systematic study of D. huoshanense and should be paid more attention.

These findings provide guidance for further research on D. huoshanense and encourage researchers to develop new functions and utilization.

Statements

Author contributions

LG collated the literature and wrote the manuscript. FW and TH helped to classify the literature and prepare the table. CG and TX contributed to performing the arrangement of pictures and checking the chemical structures. BH and DL supervised and reviewed the manuscript. All authors have approved the final version.

Funding

This work was supported by the China Agriculture Research System (CARS-21), key projects of excellent young talents support program of Anhui universities (gxyqZD2020040), and Natural Science Foundation of Higher Education Institutions of Anhui Province (KJ2021A0951). We also acknowledged the funding sponsored by the Administration of Traditional Chinese Medicine of Anhui Province (2020ccyb09) and West Anhui University (WGKQ2021022, WXZR202030, WGKQ202001012).

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors, and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Glossary

  • AGEs

    advanced glycation end products

  • AGEs

    advanced glycation end products

  • ALT

    alanine aminotransferase

  • AST

    aspartate aminotransferase

  • CAT

    catalase

  • CSA-Pyr method

    chlorosulfonic acid-pyridine method

  • D. chrysotoxum

    Dendrobium chrysotoxum

  • D. huoshanense

    Dendrobium huoshanense

  • D. moniliforme

    Dendrobium moniliforme

  • D. nobile

    Dendrobium nobile

  • D. officinale

    Dendrobium officinale

  • GC-MS

    gas chromatography-mass spectrometry

  • GSH

    L-glutathione

  • GSH-PX

    glutathione peroxidase

  • GST

    glutathione S-transferase

  • GR

    glutathione reductase

  • HMBC

    heteronuclear multiple bond correlation

  • H2O2

    Hydrogen peroxide

  • HPLC-GPC

    high performance gel permeation chromatography

  • HSQC

    heteronuclear singular quantum correlation

  • IFN-γ

    interferon-γ

  • IL-1β

    interleukin-1β

  • IL-12 p40

    interleukin 12 p40

  • IL-10

    interleukin 10

  • IL-6

    interleukin 6

  • iNOS

    inducible nitric oxide synthase

  • IR

    infrared radiation

  • LDH

    lactate dehydrogenase

  • MAPKs

    mitogen-activated protein kinases

  • MCP-1

    monocyte chemoattractant protein-1

  • MDA

    malonaldehyde

  • MIP-2

    macrophage inflammatory protein-2

  • NF-κB

    nuclear factor-kappa B

  • NK cells

    natural killer cells

  • NKT cells

    natural killer T cells

  • NMR

    nuclear magnetic resonance

  • NO

    nitric oxide

  • 8-OhdG

    8-hydroxy-20-deoxyguanosine

  • PI3 K/Akt

    phosphoinositide 3-kinase-akt

  • p-IκBα

    phosphorylated IκBα

  • SOD

    superoxidedismutase

  • TGF-β1

    transforming growth factor β1

  • TNF-α

    tumor necrosis factor-α

  • T-AOC

    total antioxidant capacity

References

Summary

Keywords

Dendrobium huoshanense, phytochemistry, pharmacology, materia medica research, resource distribution

Citation

Gao L, Wang F, Hou T, Geng C, Xu T, Han B and Liu D (2022) Dendrobium huoshanense C.Z.Tang et S.J.Cheng: A Review of Its Traditional Uses, Phytochemistry, and Pharmacology. Front. Pharmacol. 13:920823. doi: 10.3389/fphar.2022.920823

Received

15 April 2022

Accepted

30 May 2022

Published

12 July 2022

Volume

13 - 2022

Edited by

Michael Heinrich, University College London, United Kingdom

Reviewed by

Mark Arcebal Kling Naive, Jose Rizal Memorial State University, Philippines

Paromik Bhattacharyya, Institute of Himalayan Bioresource Technology (CSIR), India

Jian-Ping Luo, Hefei University of Technology, China

Destario Metusala, National Research and Innovation, Agency, Indonesia

Updates

Copyright

© 2022 Gao, Wang, Hou, Geng, Xu, Han and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Leilei Gao, ; Bangxing Han, ; Dong Liu,

This article was submitted to Ethnopharmacology, a section of the journal Frontiers in Pharmacology

Disclaimer

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

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