Abstract
Nephropathy is a general term for kidney diseases, which refers to changes in the structure and function of the kidney caused by various factors, resulting in pathological damage to the kidney, abnormal blood or urine components, and other diseases. The main manifestations of kidney disease include hematuria, albuminuria, edema, hypertension, anemia, lower back pain, oliguria, and other symptoms. Early detection, diagnosis, and active treatment are required to prevent chronic renal failure. The concept of nephropathy encompasses a wide range of conditions, including acute renal injury, chronic kidney disease, nephritis, renal fibrosis, and diabetic nephropathy. Some of these kidney-related diseases are interrelated and may lead to serious complications without effective control. In serious cases, it can also develop into chronic renal dysfunction and eventually end-stage renal disease. As a result, it seriously affects the quality of life of patients and places a great economic burden on society and families. Ginsenoside is one of the main active components of ginseng, with anti-inflammatory, anti-tumor, antioxidant, and other pharmacological activities. A variety of monomers in ginsenosides can play protective roles in multiple organs. According to the difference of core structure, ginsenosides can be divided into protopanaxadiol-type (including Rb1, Rb3, Rg3, Rh2, Rd and CK, etc.), and protopanaxatriol (protopanaxatriol)- type (including Rg1, Rg2 and Rh1, etc.), and other types (including Rg5, Rh4, Rh3, Rk1, and Rk3, etc.). All of these ginsenosides showed significant renal function protection, which can reduce renal damage in renal injury, nephritis, renal fibrosis, and diabetic nephropathy models. This review summarizes reports on renal function protection and the mechanisms of action of these ginsenosides in various renal injury models.
1 Introduction
Nephropathy is a general term for kidney-related diseases, which refers to changes in the structure and function of the kidney caused by various factors, resulting in pathological damage to the kidney, abnormal blood or urine components, and other diseases. The main clinical manifestations of nephrosis are hematuria, albuminuria, edema, hypertension, anemia, lower back pain, oliguria, and other symptoms. Nephropathy can be divided into glomerular, renal tubular, renal interstitial, and vascular diseases according to the main components of the disease. Chronic renal diseases may eventually lead to chronic renal failure. Renal disease can be divided into acute renal injury and chronic renal failure according to the degree, reversibility, and time of occurrence of renal function damage. Nephropathy can be divided into primary and secondary nephropathy. Primary nephrosis includes immune reaction-mediated nephritis, infectious diseases of the urinary system, renal vascular diseases, renal stones, renal tumors, and congenital nephrosis. Secondary nephropathy can be induced by tumors, metabolism, autoimmune diseases, and other diseases, which are also observed with various drugs, toxins, and other damage to the kidney. Acute kidney injury (AKI) is a prevalent critical renal disease associated with a high risk of death in hospitalized patients. Data from 2015 showed that both the incidence of AKI and mortality after AKI in inpatients exceeded 20% and the prognosis of AKI did not improve significantly (Mehta, et al. 2015). Recent studies have confirmed that renal function cannot completely recover or even require long-term renal replacement therapy in some patients with AKI. AKI eventually progresses to chronic kidney disease (CKD) or end-stage renal disease (ESRD) (Bucaloiu, et al. 2012). Nephritis is one of the most common kidney-related diseases. In clinical practice, glomerulonephritis (GN) is usually referred to as nephritis, which is an immune reaction disease (Coresh, et al. 2003). Renal fibrosis (RF) is a pathophysiological change that is a progressive process of renal function, deteriorating from health to injury until loss of function occurs. RF is also involved in the terminal pathway in CKD and nephritis. The therapeutic effects of CKD and nephritis are closely related to the degree of RF (Gewin, et al. 2017). Diabetic nephropathy (DN) is another major category of kidney-related disease. DN manifests as glomerulosclerosis, caused by diabetic microangiopathy. Once DN develops, most patients develop ESRD (Reutens and Atkins, 2011). Nephropathy, especially CKD and DN, has become a global public health issue with increasing annual prevalence. These urgent problems have prompted researchers to develop more drugs and methods for the treatment of nephropathy.
In recent years, a class of chemical drugs, including selective endothelin receptor antagonists and phosphodiesterase inhibitors, have been used to improve renal function because of their effects on reducing fasting blood glucose and glycosylated hemoglobin levels, as well as anti-inflammatory and anti-fibrosis effects. However, through the summary of previous clinical experience, it was found that some drugs are very easy to produce a variety of adverse reactions in the process of use, and can induce urinary tract infections, thus reducing the efficiency of clinical treatment. After a series of research experiments medical experts found that Chinese medicine plays an important role in the treatment of the disease, and Chinese medicine can regulate blood sugar as well as lipid metabolism to minimize kidney damage, thus creating favorable conditions for promoting early recovery.
Ginsenosides are the main active components of the traditional Chinese herbal medicine Panax ginseng C. A. Mey (Song, et al. 2022). At present, nearly 200 ginsenosides have been isolated and identified from the roots, stems, leaves, flower buds, and berries of Panax ginseng C. A. Mey. (Zhao, et al. 2022a). Ginsenosides can be divided into three classes according to their aglycone structure: protopanaxadiol (PPD), protopanaxatriol (PPT), and oleanolic acid. The PPD types mainly include ginsenosides Rb1, Rd, Rg3, Rh2, CK, and F2, etc., PPT types mainly include ginsenosides Re, Rf, Rg1, Rg2, Rh1, and F1, etc., and oleanolic acid types mainly include ginsenoside Ro (Hou, et al. 2021, Liu, et al. 2022). Modern pharmacological studies have shown that ginsenosides have neuroprotective (Zarneshan, et al. 2022), anti-aging (Meng, et al. 2022), anti-oxidant (He, et al. 2022a), anti-inflammatory (Xu, et al. 2022), and anti-cancer (Zhao, et al. 2022b), effects (Figure 1). Nowadays, many renal protective drugs have been widely used, but some of them have potential adverse effects. Monomers extracted from traditional Chinese herbal medicines have attracted considerable attention as effective and safe substitutes for kidney diseases (Gao, et al. 2017). Ginsenoside has been proven to have significant renoprotective effects and can be used as an antioxidant, anti-inflammatory, and anti-apoptotic agent. This review summarizes the protective effects and molecular mechanisms of ginsenosides in various types of kidney diseases.
FIGURE 1
2 Nephropathy
Nephropathy is a general term for common kidney diseases that seriously endanger human health, including different types of renal injury, renal failure, nephritis, renal fibrosis, and DN. The pathogenesis of kidney disease is complex and often involves multiple mechanisms, as shown in Figure 1. Different types of kidney diseases may also interact and progress. Here, the characteristics of these kidney-related diseases are introduced.
2.1 Renal injury
Acute kidney injury (AKI) is a common clinical emergency characterized by a rapid decline in renal function, which eventually leads to acute renal failure (ARF) and other organ failures. The etiology of AKI is complex and diverse and can be divided into prerenal, renal, and postrenal according to the anatomical location of the etiology. Prerenal AKI refers to a progressive decrease in blood flow perfusion in the renal parenchyma due to various causes, leading to a progressive decrease in the glomerular filtration rate (GFR). Renal AKI refers to renal parenchymal damage caused by a variety of factors, including the unrelieved renal ischemia of pre-renal AKI and damage to glomeruli, renal tubules, renal interstitium, and renal microvessels. Postrenal AKI refers to urinary tract obstruction caused by many factors and can be generally divided into intrarenal, extrarenal, and urethral obstruction (Chen and Guo, 2019). In recent years, the incidence of AKI has been increasing, and the incidence in hospitalized patients which has reached 1%–5%, has been growing rapidly. Once AKI occurs, the fatality rate of patients increases significantly, and the death rate of severe cases is >50% (Weiyun, et al. 2019).
Chronic kidney disease (CKD) is a disease of chronic renal insufficiency caused by various primary or secondary causes of renal injury and is characterized by chronic glomerular and renal tubule injury. CKD is defined as persistent urinary abnormalities, structural abnormalities, or impaired renal function, suggesting loss of functional nephrons (Romagnani et al. 2017; Diwan et al. 2018). The basic clinical manifestations of CKD include proteinuria, hematuria, hypertension, and edema. CKD pathogenesis mainly involves immune responses, cytokines, inflammatory mediators, and hemodynamic abnormalities.
Previously, it was believed that the kidney had a strong compensatory capacity, and the renal function of patients with AKI could recover better. However, recent studies have confirmed that the renal function of a considerable number of AKI patients that cannot be completely recovered, may even require long-term renal replacement therapy, eventually progressing to CKD or ESRD (Lo et al. 2009; Chawla et al. 2014). The important pathogenic mechanisms of AKI progression to CKD include glomerular hyperfiltration and hypertrophy, mitochondrial dysfunction (Zhan et al. 2013), cell infiltration and secretion of bioactive molecules (Venkatachalam et al. 2010), reduction in renal capillary density, and tubulointerstitial fibrosis (Chawla and Kimmel, 2012). Cytokines such as ET-1 (López-Farré, Gómez-Garre, et al. 1991), TGF- β (Gentle, et al. 2013), serum galectin-3 (Calvier, et al. 2013), and HIF (Nangaku, et al. 2013), play a role in these pathways.
Some chemotherapeutic drugs and chemical reagents for diagnosis and treatment may lead to drug-induced AKI. As a highly effective and broad-spectrum anticancer drug, the renal transport of cisplatin (CDDP) is regulated by proximal tubular transporters, which accumulate in proximal tubular epithelial cells, causing inflammation, injury, and cell death. More than a third of the patients receiving CDDP treatment suffer from nephrotoxicity, manifested as AKI, loss of serum sodium and magnesium, and dysfunction of the urine concentration (Miller, et al. 2010). AKI is the main complication of CDDP-induced nephrotoxicity.
2.2 Nephritis
In a narrow sense, nephritis refers to glomerulonephritis, which is generally referred to as nephritis in clinical practice. Broadly, nephritis includes pyelonephritis, glomerulonephritis (GN), and tubulointerstitial nephritis. Pyelonephritis is an inflammation of the renal pelvis and renal parenchyma caused by pathogenic microorganisms and is often accompanied by lower urinary tract infections. Glomerulonephritis, a disease caused by an immune reaction, is commonly referred to as nephritis and is mainly located in the glomerulus. Interstitial nephritis, also known as tubulointerstitial nephritis, is a clinical-pathological syndrome of acute and chronic renal tubulointerstitial damage caused by various factors.
Anti-glomerular basement membrane (GBM) nephritis is an autoimmune disease associated with the GBM antibody (Lahmer and Heemann, 2012). Nephritis belongs to type one rapidly progressive glomerulonephritis, the pathological classification of which is crescentic nephritis. Most patients have an acute onset, rapid progression, and poor prognosis (Fernandes, et al. 2016). Most untreated patients die of acute renal failure or pulmonary hemorrhage. The onset of the disease is characterized by rapid progressive nephritis syndrome.
Lupus nephritis (LN) is an immune injury caused by systemic lupus erythematosus (SLE) in different pathological kidney types. The clinical manifestations of LN are mostly similar to those of nephrotic syndrome or chronic glomerulonephritis, with edema, hematuria, proteinuria, hypertension, fever, rash, and other symptoms (Yung et al. 2017; Lin et al. 2019). LN is one of the main complications and lethal factors of SLE. LN pathogenesis mainly includes immune complex lesions (Anders and Fogo, 2014) and epigenetic abnormalities (Dieker, et al. 2007). In addition, abnormalities in the complement system (Hristova and Stoyanova, 2017), sexual hormone disorders (Feng, et al. 2010), and environmental effects are also related to the occurrence and development of LN.
2.3 Renal fibrosis
Renal fibrosis (RF) is a pathological result of long-term or repeated renal injury caused by single or multiple factors, the main pathological feature of which is excessive deposition of the extracellular matrix. The microscopic manifestation of RF is fibrosis of intrinsic renal cells, which is essentially the necrosis of intrinsic renal cells due to damage (Ke, et al. 2015). RF is the terminal stage of several chronic kidney diseases. It is a pathological process characterized by leukocyte infiltration, apoptosis, necrosis of renal tubular cells, the proliferation of tubulointerstitial fibroblasts, and deposition of extracellular matrix (Liu, 2010). Studies have shown that in addition to epithelial damage, the mechanism of RF is related to growth factors, such as transforming growth factor β (TGF-β) (Liu 2006), platelet-derived growth factor (Ostendorf, et al. 2014), connective tissue growth factor (Burns, et al. 2006), and epidermal growth factor (Stangou, et al. 2009). Renal fibrosis can be divided into two stages depending on the extent of damage to the intrinsic cells of the kidney and whether they can be repaired namely the reversible stage of fibrosis formation and progression, and the scar formation stage. The treatment of the first stage is of great significance for the rehabilitation of kidney disease and reversal of renal failure, which should be urgently addressed by doctors and patients. In the second stage, although it is possible to prevent the progression of renal fibrosis, it is difficult to repair scarred renal tissue (Humphreys, 2018).
2.4 Diabetic nephropathy
More than 30% of patients with diabetes suffer from DN, which is the main cause of morbidity and mortality. DN is characterized by early microalbuminuria, which gradually develops into massive albuminuria and progressive renal insufficiency, and finally forms ESRD. Unfortunately, once ESRD develops, the 5-year survival rate of patients is usually less than 20% (Natesan and Kim, 2021). The main pathological features of DN include glomerulosclerosis, tubulointerstitial fibrosis, and renal vascular disease. The pathogenic factors and pathogenesis are complex and include metabolic disorder (Xu, et al. 2018; Chen, et al. 2020), genetic factors (Wu, et al. 2021a), oxidative stress (Stehouwer, 2004; Sagoo and Gnudi, 2018), and inflammatory mechanisms (Wada and Makino, 2013), etc.
3 Application of ginsenosides in kidney related diseases
Ginsenosides are usually composed of 30 carbon atoms, with a 4-ring steroid structure and a sugar group. The history of separating ginsenosides from plants (e.g., ginseng, Panax notoginseng, American ginseng) can be traced back to 1854. More than 100 types of ginsenosides have been identified and successfully classified (Shin, et al. 2015). Each ginsenoside has at least two (C-3 and C-20) or three (C-3, C-6, and C-20) hydroxyl groups, which are free or linked to monomers, disaccharides, or trimers in most cases (Shin and Oh, 2016). The variety of ginsenosides is due to the different positions and the number of glycosyl groups connected by triterpene saponins, stereoisomerism of ginsenosides, and variable side chains at the C-20 position. Furthermore, it has created a variety of active ingredients, including anti-cancer, anti-diabetes, anti-fatigue, anti-aging, liver protection, and for kidney protection (Yenisetti et al. 2016). According to the position of the sugar group at C-3 and C-6, ginsenosides are divided into three categories: protopanaxadiol (PPD)-type saponins, protopanaxatriol (PPT)-type saponins, and oleanolic acid-type saponins (others) (Qu, et al. 2009), as shown in Figure 2. Ginsenosides with a high content of ginseng, such as Re, Rg1, Rd, and Rb1, contain many sugar residues, which makes it impossible or difficult to be directly absorbed and utilized by the human body after ingestion. These ginsenosides usually need to be metabolized and converted into smaller molecules before being absorbed by the human body, which greatly affects their biological activities. After chemical or biological transformation, the main ginsenosides can be metabolized into rare ginsenosides such as Rg3, Rk1, Rg5, CK, Rk3, and Rh4. These rare saponins show higher pharmacological activity owing to the reduction of sugar residues linked to their molecular structure, increased hydrophobicity, and enhanced cellular penetration. In addition, a variety of ginsenosides have been shown to exert anti-cancer, anti-diabetic, anti-fatigue, anti-aging, hepatoprotective and renoprotective effects (Yenisetti et al. 2016). Numerous studies have shown that ginsenosides can protect the kidneys from damage through different pathways. For example, Ginsenoside Rb1 can treat acute kidney injury by activating the Nrf2/ARE pathway (Sun et al. 2012). The mechanisms of ginsenosides to alleviate renal diseases by improving glucolipid metabolism, inhibiting oxidative stress, anti-inflammation, anti-apoptosis, regulating autophagy, and anti-fibrosis are highlighted here, as shown in Table 1.
FIGURE 2
TABLE 1
| Type | Ginsenosides | Nephropathy | Mechanism of drug action | References |
|---|---|---|---|---|
| PPD | Rb1 | AKI | Activating the Nrf2/ARE pathway | Sun et al. (2012) |
| CKD | Reducing oxidative stress and inflammation | Zhang et al. (2022) | ||
| CKD | Inhibiting the Wnt/β-catenin pathway | Xu et al. (2017) | ||
| CKD | Regulation of Akt-independent and AMPK-dependent mTOR signaling to inhibit autophagy | Zhou et al. (2019) | ||
| RF | Downregulation of TGF-β1 expression | Xie et al. (2009) | ||
| RF | Inhibiting Bip/eIF2α/CHOP signaling-mediated EMT | Ni et al. (2022) | ||
| Type 2 DN | Regulating the expression of miR-3550 and further combining with Wnt/β-catenin signaling | Shao et al. (2019) | ||
| Type 2 DN | Inhibiting aldose reductase activity | He et al. (2022a) | ||
| Rb3 | cisplatin-induced AKI | Regulating AMPK-/mTOR-mediated autophagy and inhibiting apoptosis | Xing et al. (2019) | |
| cisplatin-induced AKI | TGF-β-mediated mitochondrial apoptosis | Wu et al. (2021a) | ||
| Rg3 | D-galactose induced AKI | Inhibiting the renal oxidative stress caused by D-galactose, and at the same time activated the PI3K/AKT signaling pathway to attenuate the apoptosis of liver and kidney cells | Sun et al. (2013) | |
| LPS-induced AKI | Reducing the expression of NF-kB and iNOS proteins, and reduced the expression of COX-2 and HO-1 proteins | Kang et al. (2007) | ||
| cisplatin-induced AKI | Regulation of PI3K/AKT and NF-κB-mediated apoptosis and inflammatory pathways | Zhang et al. (2021) | ||
| cisplatin-induced AKI | Inhibiting NLRP3 by inhibiting apoptosis and autophagy | Zhai et al. (2021) | ||
| cisplatin-induced AKI | Blocking the JNK-p53-caspase-3 signaling cascade | Han et al. (2016) | ||
| cisplatin-induced AKI | Regulating inflammation and apoptosis | Park et al. (2015) | ||
| Type 2 DN | Regulation of MAPK/NF-κB signaling pathway | Li et al. (2021) | ||
| Type 2 DN | Inhibit inflammation | Zhou et al. (2020) | ||
| Type 2 DN | Inhibiting oxidative stress and advanced glycation end product formation | Kang et al. (2010b) | ||
| kidney cancer | Blockade of TRPM7 channel activity | Kim et al. (2011) | ||
| Rd | cisplatin-induced AKI | Inhibiting free radical-mediated lipid peroxidation while inhibiting apoptosis | Yokozawa and Dong (2001) | |
| cisplatin-induced AKI | Inhibition of lipid peroxidation by free radicals | Yokozawa and Liu (2000) | ||
| kidney cancer | Inhibiting TRPM7 channel activity | Kim et al. (2013) | ||
| Rh2 | cisplatin-induced AKI | Acting on a caspase-mediated pathway | Qi et al. (2019) | |
| Type 2 DN | Down-regulating discoid domain receptor 1 | Shen et al. (2021) | ||
| CK | Primary GN | Enhancing autophagy induction by inhibiting NLRP3 inflammasome activation in kidney tissue, macrophages, and bone marrow-derived dendritic cells, increasing SIRT1 expression, and triggering autophagy-mediated NLRP3 inflammasome inhibition | Wu et al. (2020) | |
| TIN | Inhibiting NLRP3 inflammasome initiation and mitochondria-related activation signaling in tubulointerstitial lesions | Hsu et al. (2020) | ||
| Type 2 DN | Inhibiting NLRP3 inflammasome activation and NF-κB/p38 signaling pathway in diabetic nephropathy in high-fat diet/streptozotocin-induced diabetic mice | Song et al. (2018) | ||
| Type 2 DN | Enhancing antioxidant capacity, reduced the damage of TGF-β1 to renal tissue | Shao et al. (2015) | ||
| CK | kidney cancer | Regulating ROS and LNRNA THOR | Chen et al. (2021) | |
| PPT | Rg1 | AKI | Inhibition of sideroporosis in renal TEC by FSP1 | Guo et al. (2022) |
| D-galactose induced AKI | Preventing DNA damage by attenuating oxidative stress | Fan et al. (2016) | ||
| CKD | Inhibiting NOX4-NLRP3 signaling in mice | Liu (2010) | ||
| Anti-GBM RPGN | Increasing renal blood flow | Hattori et al. (1991) | ||
| Anti-GBM RPGN | Activating NRF2 signaling | Guo et al. (2019) | ||
| RF | Downregulation of TGF-β1 expression | Xie et al. (2008a) | ||
| RF | Blocking TEMT by inhibiting the expression of TSP-1, thereby inhibiting the activation of TGF-β1 | Xie et al. (2008b) | ||
| RF | Inhibiting endoplasmic reticulum stress-induced apoptosis in rats after unilateral ureteral obstruction | Li et al. (2015) | ||
| RF | Inhibiting TGF-β1-induced transdifferentiation of rat renal tubular epithelial cells | Xie et al. (2008c) | ||
| RF | Inhibiting NOX4 and NLRP3 inflammasome activation in SAMP8 mice | Shen et al. (2020) | ||
| RF | Regulating Klotho/TGF-β1/Smad signaling pathway | Li et al. (2018) | ||
| Type 2 DN | Regulating the PI3K/AKT/FOXO3 pathway | Liu et al. (2021) | ||
| Type 2 DN | Reducing the expression of TGF-β1 and the already mentioned inflammatory response factors in renal tissue | Ma et al. (2010) | ||
| Type 2 DN | Reducing the expressions of TNF-α and MCP-1 | Zhang et al. (2009) | ||
| Type 2 DN | Reducing oxidative stress and inhibits TGF-β1/Smads signaling cascade in renal fibrosis in diabetic nephropathy rats | Du et al. (2018) | ||
| Rg2 and Rh1 | AKI | Blockade of LPS-TLR4 signaling reduced p38-STAT1 activation and NF-κB translocation, which in turn suppressed the transcription of inflammatory cytokines and mediators such as IFN-β, TNF-α, IL-1β, and iNOS | Huynh et al. (2020) | |
| Rh1 | Type 2 DN | Regulation of AMPK/PI3K/Akt-mediated inflammatory and apoptosis signaling pathways | Su et al. (2021) | |
| Others | Rg5 | cisplatin-induced AKI | Inhibiting inflammation, oxidative stress and apoptosis | Li et al. (2016) |
| cisplatin-induced AKI | Regulating inflammation and apoptosis | Park et al. (2015) | ||
| Type 2 DN | Inhibiting NLRP3 inflammasome activation and MAPK signaling pathway in high-fat diet/streptzotocin-induced diabetic mice | Zhu et al. (2020) | ||
| Rh3 | kidney cancer | Inhibition of the JNK and ERK mitogen-activated protein kinase signaling cascades | Lee and Kang (2017) | |
| Rh4 and Rk3 | cisplatin-induced AKI | anti-oxidation | Baek et al. (2006) |
The mechanism of action of various ginsenosides on different kidney related diseases.
3.1 PPD type ginsenosides
The PPD type ginsenoside uses PPD as an aglycone. Generally, ginsenosides are divided into two configurations: 20 S)—PPD and 20 R)—PPD, according to the substitution of C-3 or C-20 hydroxyl groups of aglycones by different sugar groups. The structural formulas of common PPD-type ginsenosides are shown in Figure 1. The PPD ginsenoside group has been shown to have significant pharmacological activities, including ginsenosides Rb1, Rb2, Rb3, RC, Rg3, Rh2, Rd, and CK (Table 2). PPD has good biological activities such as antioxidant and anti-inflammatory (Yang et al. 2019). Several studies have reported the nephroprotective effects of PPD and related mechanisms.
TABLE 2
| Ginsenoside | Activity | References |
|---|---|---|
| Rb1 | Neuroprotective, antioxidant, estrogen-like effects | Lee et al. (2003a) |
| Park et al. (2005) | ||
| Rb2 | Inhibition of tumor metastasis | Fujimoto et al. (2001) |
| Rb3 | Antioxidative | Liu et al. (2002) |
| Liu et al. (2003) | ||
| RC | Enhance immunity, anti-inflammatory effect | Berek et al. (2001) |
| Surh et al. (2002) | ||
| Rg3 | Anti-tumor, nerve protection, blood vessel protection, anti-platelet aggregation | Popovich and Kitts (2002) |
| Keum et al. (2003) | ||
| Rd | Enhance immunity, antioxidant, protect cardiovascular and cerebrovascular | Berek et al. (2001) |
| Liu et al. (2002) | ||
| Liu et al. (2003) | ||
| Rh2 | Antitumor | Bae et al. (2004) |
| Kim and Jin (2004) | ||
| CK | Anti - heritable virus effect, anti—tumor | Lee et al. (1998) |
| Lee et al. (2005) |
Pharmacological activity of PPD type ginsenosides.
3.1.1 Ginsenoside Rb1
Ginsenoside Rb1 is one of the main active monomers of ginseng and has the ability to scavenge oxygen free radicals and thus has antioxidant activity. Ginsenoside Rb1 has been shown to inhibit renal ischemia-reperfusion injury and interstitial fibrosis and reduce renal cell apoptosis and oxidative damage. For instance, ginsenoside Rb1 upregulates Nrf2 and heme oxygenase-1 (HO-1) by activating the nuclear factor-related factor 2 (Nrf2)/ARE pathway, which in turn attenuates acute kidney injury caused by intestinal ischemia-reperfusion (Sun et al. 2012). In addition, the possible protective effects and mechanisms of ginsenoside Rb1 on oxidative damage and renal interstitial fibrosis in rats with unilateral ureteral obstruction (UUO) have been widely studied. Fan et al. found that ginsenoside Rb1 significantly inhibited renal interstitial fibrosis in UUO rats by down-regulating TGF-β1 (Xie et al. 2009) expression or ihibiting Bip/eIF2α/CHOP signaling-mediated EMT (Ni et al. 2022). There are reports that ginsenoside Rb1 treats acute kidney injury by activating the Nrf2/ARE pathway which acts against oxidative stress (Sun et al. 2012). Ginsenoside Rb1 can further prevent autophagy by inhibiting the Wnt/β-catenin pathway (Xu, et al. 2017) or by regulating Akt-independent (cell proliferation and survival) and AMPK-dependent mTOR signaling-involved in cell survival under energy stress (Zhou et al. 2019), thereby reducing oxidative stress and inflammation in patients with CKD (Zhang, et al. 2022). In detail, ginsenoside Rg1 treatment significantly reduced ROS production and inhibited NOX4 and NLRP3 inflammatory vesicle activation, which in turn ameliorated LPS-induced chronic kidney injury and renal fibrosis (Zhang et al. 2022). Rb1 can also inhibit the Wnt/β-linked protein pathway by activating peroxisome proliferator-activated receptor γ (PPAR-γ) to exert anti-calcium properties and thus improve the symptoms of CKD. In recent years, studies have found that ginsenoside Rb1 can regulate Type two diabetic nephropathy by regulating the expression of miR-3550 and further combining with Wnt/β-catenin signaling (Shao et al. 2019) or inhibiting aldose reductase activity (He et al. 2022b).
3.1.2 Ginsenoside Rb3
Ginsenoside Rb3, which is one of the main pharmacologically active ingredients, mainly exists in the roots, flower buds, stems, and leaves of Panax ginseng; the roots, stems, and leaves of Panax quinquefolium; and the stems and leaves of Panax notoginseng. According to reports, ginsenoside Rb3 can regulate cisplatin-induced AKI by regulating AMPK/mTOR-mediated autophagy and inhibiting apoptosis in vitro and in vivo (Xing et al. 2019). Among them, Li et al. demonstrated for the first time the protective effect and potential mechanism of ginsenoside Rb3 on cisplatin-induced renal failure, restoring the antioxidant system by regulating the AMPK/mTOR signaling pathway, and inhibiting proximal tubular damage by inhibiting ROS-mediated apoptosis and autophagy. Some evidence suggests that the TGF-β pathway may lead to cisplatin-induced nephrotoxicity, and ginsenoside Rb3 can have a protective effect on nephrotoxicity in the treatment of oral cancer with CPT through TGF-β pathway-mediated mitochondrial apoptosis (Wu et al. 2021b).
3.1.3 Ginsenoside Rd
Ginsenoside Rd is a rare type of saponin. The content of Rd in ginseng is very low, while that in Panax notoginseng is approximately 0.36%–1.47%, which is higher than that in ginseng. Intestinal enzymes can metabolize Rb1 with high content into Rd; therefore, Rd is one of the important forms of saponins that are absorbed and utilized by the intestine after metabolism. Recent studies have found that ginsenoside Rd has strong biological activity, especially protective effects on the kidneys. For example, ginsenosides-Rd eliminate the damaging effects of oxidative stress on the kidneys by inhibiting free radical lipid peroxidation (Yokozawa and Liu 2000). Moreover, ginsenoside Rd can regulate cisplatin-induced AKI by inhibiting free radical-mediated lipid peroxidation while inhibiting apoptosis (Yokozawa and Dong 2001). In the research of exploring new anti-kidney cancer drugs, ginsenoside Rd has played an important role. Studies have said that ginsenoside Rd can regulate kidney cancer by inhibiting TRPM7 channel activity (Kim 2013).
3.1.4 Ginsenoside Rg3
Ginsenoside Rg3 is one of the main active substances in ginseng and has extensive pharmacological effects. With the new foci of the research, Rg3 was found to have anti-tumor effects (Sin et al. 2012; Ding et al. 2015), reducing the cardiotoxicity and nephrotoxicity of chemotherapy drugs (Han et al. 2016), and anti-cicatricial (Tang et al. 2018). Through literature reading, we find that there is a growing number of studies focusing on ginsenoside Rg3 to improve acute kidney injury. For example, Rg3 can regulate D-galactose-induced AKI by inhibiting the renal oxidative stress caused by d-galactose and simultaneously activating the PI3K/AKT signaling pathway to attenuate the apoptosis of liver and kidney cells (Sun, et al. 2013). Rg3 can regulate LPS-induced AKI by reducing the expression of NF-kB and iNOS proteins and reducing the expression of COX-2 and HO-1 proteins (Kang, et al. 2007). Numerous studies have also shown that ginsenoside Rg3 can improve cisplatin-induced AKI by modulating multiple pathways. In details, ginsenosides Rg3 can regulate cisplatin-induced AKI by regulating PI3K/AKT and NF-κB-mediated apoptosis and inflammatory pathways (Zhang et al. 2021). Ginsenosides Rg3 also reduces cisplatin-induced AKI by inhibiting apoptosis and autophagy to suppress NLRP3 (Zhai et al. 2021) and blocking the JNK-p53-cysteine asparticase-3 signaling cascade (Han et al. 2016). In addition, Rg3 can also regulate type 2 diabetic nephropathy mainly focusing on pathways that regulate MAPK/NF-κB signaling pathway (Li et al. 2021), inhibit inflammation and oxidative stress response (Zhou et al. 2020) and late glycosylation end product formation (Kang et al. 2010b). There have also been recent studies that Rg3 can regulate kidney cancer by blockading of TRPM7 channel activity (Kim et al. 2011).
3.1.5 Ginsenoside Rh2
Ginsenoside Rh2 is a rare saponin found in Panax ginseng. Rh2 was first found in red ginseng and was later isolated from American ginseng, Panax notoginseng, and other plants. Rh2 can regulate the immune, central nervous, endocrine, and cardiovascular systems, etc., and has anti-tumor, anti-allergy, anti-depression, anti-aging, and improved myocardial ischemic effects. Ginsenoside Rh2 regulates cisplatin-induced AKI by acting on a caspase-mediated pathway (Qi, et al. 2019). Rh2 regulates type two diabetic nephropathy by downregulating discoid domain receptor 1 (Shen, et al. 2021).
3.1.6 Ginsenoside CK(M1)
Ginsenoside CK does not exist in natural ginseng but is a metabolite produced by diol-type ginsenosides (such as Rb1, Rb2, and Rc) under the action of intestinal flora after oral administration (Chen, et al. 2015). CK is one of the main components of ginsenosides that play a role in the body with high biological activity, including inhibition of T Cells (Kang, et al. 2010a), promotion of tumor cell apoptosis (Cho, et al. 2009), anti-inflammation (Joh et al. 2011) and protection of the myocardium (Tsutsumi, et al. 2011). Ginsenoside CK can modulate primary glomerulonephritis by inhibiting NLRP3 inflammasome activation in renal tissue, macrophages, and bone marrow-derived dendritic cells, increasing SIRT1 expression, and triggering autophagy-mediated inhibition of NLRP3 inflammable bodies (Wu, et al. 2020). Ginsenoside CK can regulate tubulointerstitial nephritis by inhibiting NLRP3 inflammasome initiation and mitochondria-related activation signaling in tubulointerstitial lesions (Hsu, et al. 2020). Ginsenoside CK can regulate type two DN by inhibiting NLRP3 inflammasome activation and the NF-κB/p38 signaling pathway in DN in high-fat diet/streptozotocin-induced diabetic mice (Song, et al. 2018) enhancing antioxidant capacity, and reducing the damage of TGF-β1 in renal tissue (Shao, et al. 2015). Ginsenoside CK regulates kidney cancer by regulating reactive oxygen species (ROS) and Testis-associated highly conserved oncogenic long-stranded non-coding RNA (LNRNA THOR) (Chen, et al. 2021). Ginsenoside M1 can regulate acute severe lupus nephritis by inhibiting NLRP3 inflammasome activation and differentially regulating T-cell function (Lin, et al. 2019).
3.2 PPT type ginsenosides
PPT-type ginsenosides take protopanaxatriol as an aglycone. Generally, ginsenosides are divided into two configurations: 20 S)—PPT and 20 R)—PPT, according to the substitution of C-6 or C-20 hydroxyl groups of aglycones by different sugar groups. The structural formulae of common PPT-type ginsenosides are shown in Figure 1. The PPT ginsenoside group has been shown to have significant pharmacological activities, mainly Re, Rg1, Rg2, and Rh1, as shown in Table 3.
TABLE 3
| Ginsenoside | Activity | References |
|---|---|---|
| Re | Inhibits proliferation and protects nerves | Cai et al. (2022) |
| Rg1 | Neuroprotective effect, induction of apoptosis, estrogen-like effect | Chan et al. (2002) |
| Rg2 | Protection of central and peripheral nervous system | Sala et al. (2002) |
| Rh1 | Boost immunity, estrogen-like, inhibit proliferation | Popovich and Kitts (2002) |
| Lee et al. (2003b) |
Pharmacological activity of PPT type ginsenosides.
3.2.1 Ginsenoside Rg1
Among all the PPT saponins, ginsenoside Rg1 ranks second only to ginsenoside Re. In addition, studies have shown that the ginsenoside Rg1 content in Panax notoginseng is high (Rg1 accounts for 20% of the total saponins of Panax notoginseng, and Re is only 2.5%), and thus has great development value (Yang, et al. 2015). Rg1 has many functions including anti-aging, anti-oxidation, and improved immunity and memory (monakhov, Baisong et al. 2018). Ginsenoside Rg1 can regulate D-galactose-induced AKI by preventing DNA damage by attenuating oxidative stress (Fan, et al. 2016). Rg1 can regulate CKD by inhibiting NOX4-NLRP3 inflammazone signaling pathways in mice (Liu, et al. 2010). Rg1 can regulate anti-GBM GN, a rare autoimmune disease, by increasing renal blood flow (Hattori, et al. 1991) and activating NRF2 signaling (Guo, et al. 2019). Ginsenoside Rg1 can inhibit the development of renal fibrosis by modulating various pathways, such as the downregulation of protein TGF-β1 expression (Xie, et al. 2008a), blocking TEMT by inhibiting the expression of TSP-1 (Xie et al. 2008b), thereby inhibiting the activation of TGF-β1 (Li, et al. 2015), inhibiting endoplasmic reticulum stress-induced apoptosis in rats after unilateral ureteral obstruction (Xie et al. 2008c), inhibiting TGF-β1-induced transdifferentiation of rat renal tubular epithelial cells (Shen, et al. 2020), inhibiting NOX4 and NLRP3 inflammasome activation in SAMP8 mice, and regulating the Klotho/TGF-β1/Smad signaling pathway (Li et al. 2018). Ginsenoside Rg1 can inhibit the development of type 2 DN by regulating the PI3K/AKT/FOXO3 pathway (Liu, et al. 2021), reducing the expression of TGF-β1 and the already mentioned inflammatory response factors in renal tissue (Ma et al. 2010), and reducing the expression of TNF-α and MCP-1 (Zhang, et al. 2009). The combination of Rg1 and Astragalus IV can reduce oxidative stress and inhibit the TGF-β1/Smad signaling cascade in renal fibrosis in rats with DN (Du, et al. 2018).
3.2.2 Ginsenoside Rg2 and Rh1
Rg2 is an intermediate product of ginsenoside Re metabolism in vivo. Rg2 has many biological activities, such as affecting the sensitivity of the opposite nerve cell process receptors, promoting intercellular communication, and reducing the neural activity caused by electricity in the rat hippocampus (Sala, et al. 2002). Ginsenoside Rg2 and Rh1 can regulate AKI by blocking LPS-TLR4 signaling, which reduces p38-STAT1 activation and NF-κB translocation, which in turn suppresses the transcription of inflammatory cytokines and mediators, such as IFN-β, TNF-α, IL-1β, and iNOS (Huynh, et al. 2020).
3.2.3 Ginsenoside Rh1
Ginsenoside Rh1 is a rare saponin found in red ginseng, Panax notoginseng, and American ginseng in trace (Jeon, et al. 2020). Because of its remarkable immunoregulatory activity, Rh1 has high medicinal value in the treatment of many senile diseases (Tam, et al. 2018). In addition, Rh1 can inhibit inflammatory reaction (Vinh et al. 2017), regulate abnormal immune responses in hypersensitive disease (Han and Kim, 2020), and inhibit tumor cell proliferation (Yi, 2019). Ginsenoside Rh1 can regulate type two DN by regulating oxidative stress, angiotensin II (Ang-II), and inflammatory processes, as well as AMPK/PI3K/Akt-mediated inflammatory and apoptosis signaling pathways (Su, et al. 2021).
3.3 Other ginsenosides
Other types of ginsenosides include dammarane-type tetracyclic triterpenoids. Some of these saponins are isolated from ginseng with very little content, whereas others are obtained after chemical treatment. Compared with the original PPD- and PPT-type ginsenosides, only the skeleton of aglycone or the side chain of the parent nucleus was partially changed.
3.3.1 Ginsenoside Rg5
Ginsenoside Rg5 is a derivative of ginsenoside, one of the main components of red ginseng (Kim, et al. 1996). Rg5 is a secondary saponin obtained from PPD-type saponins (Rb1, Rb2, Rb3, Rc, and Rd) via regioselective hydrolysis and stereoselective dehydration (Lee, et al. 2009). In animal and human clinical trials, Rg5 not only has significant effects in improving lung inflammation (Kim et al. 2012) and improving memory (Yao et al. 2014), anti-cancer (Liang et al. 2015), but also reduces cisplatin induced nephrotoxicity (Li et al. 2016). Ginsenoside Rh3 can regulate cisplatin-induced AKI by inhibiting inflammation, oxidative stress, and apoptosis (Li et al. 2016). Ginsenoside Rh3 can inhibit the development of type two diabetic nephropathy by inhibiting NLRP3 inflammasome activation and the MAPK signaling pathway in high-fat diet/streptozotocin-induced diabetic mice (Zhu, et al. 2020).
3.3.2 Ginsenoside Rh3
Ginsenoside Rh3 is a metabolite of ginsenoside Rg5 in the human body (Kim et al. 2013), but it has better effects on various pharmacological activities than Rg5 (Shin et al. 2006). Ginsenoside Rh3 can regulate cisplatin-induced AKI by inhibiting JNK and ERK mitogen-activated protein kinase signaling cascades (Lee and Kang, 2017).
3.3.3 Ginsenoside Rg3, Rg5 and Rk1
Black ginseng, a new processed product of ginseng, has a unique processing method (Gao, et al. 2017). Black ginseng contains rare saponins that are different from ginseng and red ginseng, and its representative active components are Rg3, Rg5, and Rk1 (Gao, et al. 2017). As a tetracyclic triterpene discovered in recent years, Rk1 has attracted much attention because of its biological activities, such as antitumor, blood glucose regulation, and nervous system protection (Gao, et al. 2017). Ginsenoside Rk1 regulates cisplatin-induced AKI by regulating inflammation and apoptosis (Park, et al. 2015).
3.3.4 Ginsenoside Rh4 and Rk3
Ginsenoside Rk3/Rh4 comprises a pair of isomers, which are obtained by removing a sugar group from ginsenoside Rg1 and converting it to Rh1, and then removing a water molecule at C20 position from Rh1. Ginsenoside Rh4 and Rk3 can regulate cisplatin-induced AKI by inhibiting oxidation (Baek, et al. 2006).
4 Prospect and conclusion
Nowadays, kidney-related diseases have become common worldwide, such as diabetic nephropathy, chronic kidney disease, acute kidney disease, and hypertensive nephropathy. The causes of kidney diseases are complex, and since the pathogenesis of most kidney diseases is not fully understood, the treatment of kidney diseases is mostly empirical and lacks etiologic treatment tools (Liu and Xiao 1992). At the same time, many drugs that protect the kidney have been widely used, but some of them have strong adverse effects. For this reason, monomeric components derived from traditional Chinese herbal medicines have received much attention as effective and safe alternative drugs for kidney diseases. Currently, ginsenosides as natural drugs have been widely approved to exert therapeutic effects on kidney-related drugs in vivo and in vitro. In the existing studies, we found that ginsenosides for the treatment of nephropathy mainly focus on mechanisms through the inhibition of inflammatory responses and oxidative stress. Emerging reports suggest that ginsenosides can have some binding activity to the glucocorticoid receptor and can promote its nuclear translocation, while some ginsenosides can exert anti-inflammatory effects by inhibiting the activity of NF-κB. Recent findings have confirmed that NF-κB is closely associated with foot cell injury. This shows that NF-κB pathway is a hot pathway for kidney disease research. Current research mainly focuses on ginsenosides, with little research on other components such as ginsenosides polysaccharides, which should be more widely explored in depth. The efficacy of ginsenosides in the treatment of kidney disease has become clear, and in the future, in-depth research on ginsenosides in the treatment of kidney disease can be conducted from multiple angles, levels and directions to provide better treatment for patients.
In conclusion, the pathogenesis of several major kidney-related diseases is discussed in this review. The mechanism of ginsenosides in various types of nephropathy has been summarized, including PPD and PPT types. Although ginsenosides have been studied in nephropathy, their mechanism of action has not been fully elucidated. The animal model of nephropathy was used as the basis for further research and discussion of the pathogenesis and mechanism of drug action. The establishment of animal models of nephropathy should be consistent with the pathology and course of kidney disease, which is a goal of researchers. Therefore, it is necessary to further study other related effects of ginsenosides on kidney-related diseases through appropriate animal models, which are expected to develop new drugs for the clinical treatment of drug-induced nephrotoxic diseases, diabetic nephropathy, renal fibrosis, and other kidney diseases.
Statements
Author contributions
MF and XL contributed equally to this work; WG and DZ designed the review; MF, XL, QW, MS, XF, YZ, DW, and HL contributed to the writing of the manuscript.
Funding
This work was supported by the National Natural Science Foundation of China (Grant Number: 81973468), Jilin province science and technology development plan item (Grant Number: 20200708081YY), Jilin Provincial Development and Reform Commission (Grant Number: 2021C035-5).
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
The handling editor declared a past collaboration with the author MF.
Publisher’s note
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Summary
Keywords
ginsenoside, kidney, nephropathy, animal model, mechanism
Citation
Fan M, Lan X, Wang Q, Shan M, Fang X, Zhang Y, Wu D, Luo H, Gao W and Zhu D (2023) Renal function protection and the mechanism of ginsenosides: Current progress and future perspectives. Front. Pharmacol. 14:1070738. doi: 10.3389/fphar.2023.1070738
Received
15 October 2022
Accepted
24 January 2023
Published
06 February 2023
Volume
14 - 2023
Edited by
Wei Li, Jilin Agricultural University, China
Reviewed by
Dayun Sui, Jilin University, China
Jingjing Wu, Dalian Medical University, China
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Copyright
© 2023 Fan, Lan, Wang, Shan, Fang, Zhang, Wu, Luo, Gao and Zhu.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Wenyi Gao, gaowy@ccucm.edu.cn; Difu Zhu, zhudf@ccucm.edu.cn
†These authors have contributed equally to this work
This article was submitted to Ethnopharmacology, a section of the journal Frontiers in Pharmacology
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