ORIGINAL RESEARCH article

Front. Psychiatry, 13 May 2022

Sec. Anxiety and Stress Disorders

Volume 13 - 2022 | https://doi.org/10.3389/fpsyt.2022.836965

Inverse Association Between Resting-State Putamen Activity and Iowa Gambling Task Performance in Patients With Obsessive-Compulsive Disorder and Control Subjects

  • SH

    Suguru Hasuzawa 1

  • HT

    Hirofumi Tomiyama 1

  • KM

    Keitaro Murayama 1

  • AO

    Aikana Ohno 2

  • MK

    Mingi Kang 2

  • TM

    Taro Mizobe 1

  • KK

    Kenta Kato 1

  • AM

    Akira Matsuo 1

  • KK

    Kazufumi Kikuchi 3

  • OT

    Osamu Togao 3

  • TN

    Tomohiro Nakao 1*

  • 1. Department of Neuropsychiatry, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan

  • 2. Graduate School of Human Environment Studies, Kyushu University, Fukuoka, Japan

  • 3. Department of Clinical Radiology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan

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Abstract

Background:

Symptoms of obsessive-compulsive disorder (OCD) have been conceptualized as manifestations of decision-making deficits. Patients with OCD exhibit impairment during the decision-making process, as assessed by the Iowa Gambling Task (IGT). This impairment is independent of clinical severity and disease progression. However, the association between the decision-making deficit and resting-state brain activity of patients with OCD has not been examined.

Methods:

Fifty unmedicated patients with OCD and 55 matched control subjects completed IGT. Resting-state brain activity was examined using the fractional amplitude of low-frequency fluctuations (fALFFs). fALFF analysis focused on the slow-4 and 5 bands. Group comparisons were performed to determine the association between IGT performance and fALFFs.

Results:

There was a significant group difference in the association between the IGT total net score and slow-4 fALFFs in the left putamen (voxel height threshold of p < 0.001; cluster size threshold of p < 0.05; family wise error-corrected). Higher putamen slow-4 fALFFs were correlated with lower IGT scores for OCD patients (r = −0.485; p < 0.0005) and higher IGT scores for control subjects (r = 0.402; p < 0.005). There was no group difference in the association between the IGT total net score and slow-5 fALFFs.

Conclusions:

These findings in unmedicated patients demonstrate the importance of resting-state putamen activity for decision-making deficit associated with OCD, as measured by IGT. The inverse correlation may be explained by the hypersensitive response of the putamen in patients with OCD.

Introduction

Obsessive-compulsive disorder (OCD) is associated with chronic doubt and difficulty making decisions (1). Therefore, it has been suggested that OCD symptoms can be conceptualized as manifestations of a decision-making deficit. Neurocognitive research has highlighted decision-making deficits as the core feature of OCD (2–6). In this context, decision-making refers to the ability of an individual to process environmental information and choose an action that can yield a benefit. A benefit is achieved after the action as an immediate reward or after a certain period of time as a long-term reward. According to learning theory, the process during which individuals learn by trial and error to distinguish long-term rewards from immediate rewards and adjust their behaviors to maximize the ultimate benefit is called reinforcement learning (7). The Iowa Gambling Task (IGT) (8) is a well-established neuropsychological test that can assess the decision-making ability through reinforcement learning. IGT has been widely used in studies evaluating the decision-making deficits of patients with OCD (6, 9–14).

According to a meta-analysis of IGT performance in patients with OCD (15), patients perform significantly worse than control subjects (CTL). Moreover, IGT performance in patients with OCD is clinically important because it predicts the therapeutic response to serotonin reuptake inhibitors (6, 12). However, IGT performance is not necessarily correlated with the severity of overt clinical symptoms as measured by scales such as the Yale–Brown Obsessive Compulsive Scale (Y-BOCS) and Hamilton Anxiety Rating Scale (HAM-A) (6, 13, 16–18). Cavedini et al. (2) compared the poor IGT performance in depression and OCD patients and argued that the impairment observed in patients with depression is a state characteristic related to depressive mood, whereas in patients with OCD, the impairment constitutes a trait characteristic unrelated to the severity of overt symptoms. Furthermore, Zhang et al. (19) examined IGT performance in three OCD patient groups (i.e., non-medicated patients, medicated patients, and remitted patients) and found that all three groups performed worse than CTL; therefore, they concluded that the poor IGT performance in patients with OCD is trait-related. Because IGT performance in patients with OCD indicates a stable and long-lasting deficit that is not proportional to the severity of clinical phenotypes, some have regarded this performance as an endophenotype candidate for this disorder (9, 18–22).

Numerous studies have evaluated the brain activity during IGT performed by healthy individuals (23–28). IGT performance correlates with the activity of the frontal regions, including the ventromedial prefrontal cortex (vmPFC), dorsolateral prefrontal cortex (dlPFC), orbitofrontal cortex (OFC), supplementary motor area, and pre-supplementary motor area, as well as the striatum, insula, anterior cingulate cortex (ACC), and posterior cingulate cortex. These regions are involved in reward information processing. Regarding the neurobiological hypothesis of OCD, the cortico-striatal-thalamo-cortical (CSTC) loop model (29) is widely accepted, and the regions related to IGT performance largely overlap with the CSTC loops.

Nevertheless, neuroimaging studies evaluating the IGT performance in patients with OCD are limited. To the best of our knowledge, only two such studies have been conducted (30, 31), which examined the brain activity during each phase of the task; patients showed underactivation in the striatum and the frontal regions, including the vmPFC, dlPFC, and OFC, compared to controls during all three phases (i.e., outcome anticipation, decision-making, and outcome presentation). However, both of these studies have limitations. First, they targeted only adolescent patients with OCD and included patients using medication. Second, they used a shortened version of the IGT to reduce the stress that participants would experience during the simultaneous task performance and image acquisition. The administration of the shortened IGT version in the two studies resulted in no group differences in the IGT total net score.

If the poor IGT performance in patients with OCD is an endophenotype candidate for this disorder, then the abnormality might be detected in a purer form during the resting state, which is free from task load and symptom provocation. The aforementioned neuroimaging studies on IGT performance have focused mainly on task-evoked brain activation. However, if the poor IGT performance that occurs with OCD is derived from any trait-related problem, aberrations may be found in the intrinsic brain activity at rest. Moreover, performing neurocognitive assessments separately from image acquisition has the advantage of being less stressful for participants. In this case, it is not necessary to use a shortened version of IGT. Regarding other psychiatric disorders that can involve decision-making deficits (for example, pathological gambling, substance abuse, and eating disorders), some studies have revealed associations between impaired IGT performance and aberrant resting-state brain activity (32–34). However, no studies have examined the association between IGT performance and resting-state brain activity in patients with OCD.

Resting-state brain activity can be measured by the amplitude of low-frequency fluctuations (ALFFs) or fractional ALFFs (fALFFs) (35, 36). These measurements enable the assessment of the entire brain without a priori selection of regions of interest. The fALFFs comprise a relative measure of the ALFFs and are defined as the total power within a specific frequency band divided by the total power of the whole detectable frequency range. Compared to the measurement of ALFFs, which are susceptible to pulsatile artifacts, fALFF measurements have higher sensitivity and specificity (35, 36). Furthermore, in recent studies, low-frequency fluctuations were divided into several independent bands (slow-5, 0.01–0.027 Hz; slow-4, 0.027–0.073 Hz; slow-3, 0.073–0.198 Hz; slow-2, 0.198–0.25 Hz), and the features of various psychiatric disorders were investigated according to the properties of each band (37–41). Evidence has shown that the slow-2 and 3 bands are susceptible to physiological noise, such as cardiac signals, whereas the slow-4 and 5 bands can sensitively reveal neural activity (36). Slow-4 fluctuations are most robust in the basal ganglia and represent dopaminergic activities (36, 37, 42), whereas slow-5 fluctuations are more dominant than slow-4 fluctuations in the prefrontal cortices (especially in the ventromedial parts) (36, 37).

Abnormalities in fALFFs that occur with OCD have been investigated by numerous studies, but inconsistent results have been observed (40, 43–50). This inconsistency could be attributed to several factors, such as including patients using medications (40, 43, 47) and focusing exclusively on female patients (46). In contrast, Gao et al. (45) and Yang et al. (49) compared a large number of unmedicated patients with OCD and CTL and found significant group differences in fALFFs in the dlPFC, putamen, and superior frontal gyrus, as well as a negative correlation between functional connectivity in the striato-thalamic junction and symptom severity evaluated with the Y-BOCS. Because the CSTC loop model is supported by these findings of fALFF studies, it seems likely that resting-state CSTC abnormalities are related to decision-making deficit in OCD.

Here, we conducted a fALFF study of the resting-state brain activity in a large number of unmedicated adult patients with OCD and examined its relationship with IGT performance; then, we compared the results with those of CTL. Furthermore, we examined differences in fALFFs in whole-brain voxels of patients with OCD and CTL by considering the possibility of abnormalities related to decision-making deficit occurring in OCD without showing a direct correlation with IGT scores. As discussed previously, poor IGT performance in patients with OCD is thought to reflect a relatively invariable cognitive impairment, independent of the emotional disturbance (anxiety) severity. This suggests the involvement of the dorsal/cognitive part of the CSTC loops in poor IGT performance. Among the diverse brain regions involved in IGT performance in healthy individuals, the dlPFC and dorsal striatum belong to the dorsal CSTC loop. These are key regions for cognitive processing that allow individuals to learn to achieve large long-term rewards while incurring small immediate losses (51–56). In addition, the aforementioned fALFF studies on a large sample of patients with OCD (45, 49) have found abnormalities in these regions. Therefore, we hypothesized that the abnormalities responsible for the decision-making deficit associated with OCD would be found in these regions. Because slow-4 and 5 fALFFs sensitively reflect the neuroactivity of the basal ganglia and prefrontal cortices, respectively, we conducted a fALFF analysis, focusing mainly on these two bands.

Materials and Methods

Subjects

A total of 105 participants were recruited for this study, including 50 medication-free patients with OCD and 55 control subjects matched for age and sex. All patients with OCD were diagnosed using the Structured Clinical Interview for Diagnostic and Statistical Manual of Mental Disorders, fourth edition (DSM-IV), Axis I Disorders (patient edition), and fulfilled the DSM-IV criteria. We ensured that none of the participants met the criteria for any current comorbid Axis I disorder and that all participants also fulfilled the Diagnostic and Statistical Manual of Mental Disorders, fifth edition (DSM-5), criteria for OCD. The control subjects were recruited from the local community and interviewed according to the Structured Clinical Interview for DSM-IV (non-patient edition). None of the participants reported history of any psychiatric disorders. Candidates with a history of significant head injury, epilepsy, or intellectual disability were excluded.

This study was conducted in accordance with the principles of the Declaration of Helsinki. Ethical approval was obtained from the Kyushu University Ethics Committee. The study details were explained to all participants, and written informed consent was obtained from all participants.

Clinical Assessment

The participants were clinically assessed in the same manner as performed during previous studies (57–59). The Japanese version of the Y-BOCS (60) was used to assess the global severity of OCD symptoms, while the Hamilton Rating Scale for Anxiety (HAM-A) (61) and the Hamilton Rating Scale for Depression (HAM-D, 17-item version) (62) were used to quantify the degrees of anxiety and depression. The Japanese version of the National Adult Reading Test (63) was used to measure the verbal intelligence quotient (IQ). Demographic and clinical data were statistically analyzed using the chi-square test, Student's t-test, and Mann-Whitney U test to detect group differences between the OCD and CTL groups.

Neuropsychological Assessment

IGT was performed according to the original procedures developed by Bechara et al. (8); however, the hypothetical money was converted from United States dollars to Japanese yen. The participants selected cards from four decks labeled A, B, C, and D. At the start of the game, the participants were told that a loan of 200,000 yen was available. After picking a card, the participants would either win or lose some money. They could select cards from any deck, and the task was self-paced. The goal of the game was to win as much money as possible.

Card decks A and B were disadvantageous because they resulted in a net loss over time. Although they yielded larger immediate rewards, they inflicted larger penalties. In contrast, card decks C and D were advantageous because the total of the rewards was larger than that of the penalties. The participants were not informed of the risks of rewards and punishments in each deck or of the number of card selections allowed.

A total of 100 cards were selected to complete the task. Task performance was measured by the net score, which was calculated as the number of cards picked from the advantageous decks minus the number of cards picked from the disadvantageous decks.

Image Data Acquisition and Preprocessing

Magnetic resonance imaging (MRI) data were acquired in the same manner as described previously (57–59). Image data were obtained using a 3.0-T MRI scanner (Achieva TX; Phillips Healthcare, Best, the Netherlands). T2*-weighted gradient-echo planar imaging sequence (echo time, 30 ms; repetition time, 2,500 ms; field of view, 212 × 212 mm; matrix, 64 × 64; slice thickness, 3.2 mm; flip angle, 80°) and high-resolution T1-weighted anatomical image (echo time, 3.8 ms; repetition time, 8.2 ms; field of view, 240 × 240 mm; flip angle, 8°; slice thickness, 1 mm; inversion time, 1,026 ms) results were acquired for each participant. A total of 240 real scans were obtained, which involved a 10-min real scan after an initial 10-s dummy scan. The participants were instructed to relax with their eyes open and to keep watching a presented gray cross during scanning. After MRI, the arousal level during the scan was evaluated using the Stanford-Sleepiness Scale.

Resting-state functional MRI data were analyzed using the CONN toolbox 20.b (64) with MATLAB R2020a. The first four functional volumes were removed, and the remaining 236 functional images were used for analysis. The images underwent slice-timing correction, realignment, and normalization in accordance with the standard Montreal Neurological Institute template. Six rigid body parameters were estimated for each subject. We applied the ART scrubbing procedure (https://www.nitrc.org/projects/artifact_detect/) to exclude image artifacts caused by head movement using the 97th percentile as the standard threshold of a normative sample (with thresholds for motion of 0.9 mm or global bold signal changes more than 5 standard deviations). There was no significant difference in the mean motion of the OCD and CTL groups (t = −1.226; p = 0.223). Functional images were smoothed using a Gaussian kernel with 6-mm full-width at half-maximum. Using the anatomical image of each participant, we created white matter and cerebrospinal fluid masks during the spatial processing steps. Signal noises from the white matter and cerebrospinal fluid were discerned by applying linear regression as a confounding effect (64). Then, fALFFs were computed using the preprocessed data. fALFFs were defined as the ratio of power in the low-frequency range to the total power in the entire frequency range for each individual voxel time series (35). The ratios of the power in the slow-5 (0.01–0.027 Hz) and slow-4 (0.027–0.073 Hz) ranges were calculated relative to the full frequency range (0–0.25 Hz), as previously described (36).

Data Analysis

After calculating fALFFs of slow-4 and slow-5, we examined group differences in slow-4 and 5 fALFFs associated with the IGT total net score by using an analysis of covariance interaction model [statistical significance was set at a voxel height threshold of p < 0.001 and cluster-size threshold of p < 0.05; family-wise error (FWE) corrected (two-sided), with the Gaussian random field theory approach] while controlling for age, sex, and IQ, which have been reported to affect IGT performance (65–69). Additionally, we examined differences in fALFFs in whole brain voxels of the OCD and CTL groups while controlling for age, sex, and IQ [significance threshold set at a voxel height threshold of p < 0.001 and cluster size threshold of p < 0.05; FWE corrected (two-sided), with the Gaussian random field theory approach]. Furthermore, to strictly control the false-positive risk, we conducted a supplemental analysis of covariance by using a non-parametric permutation approach (5,000 times iterations), with a voxel height threshold of p < 0.001 and cluster-size threshold of p < 0.05 FWE corrected (70).

Results

Clinical Characteristics

The groups did not significantly differ in age, sex, handedness, or estimated verbal IQ (Table 1). All participants were medication-free for at least 4 weeks, and 16 patients with OCD were drug-naïve. The mean Y-BOCS score of the OCD group was 24.5 (standard deviation, ±5.51) (Table 1). During the acquisition of MRI data, no participant fell asleep, and there was no significant difference between the OCD and CTL groups in terms of the arousal level (Table 1).

Table 1

VariableOCD (n = 50)CTL (n = 55)Statistics
χ2tdfp-value
Demographic and clinical characteristics
Sex, male/female20/3024/310.14210.706
Hand, Edinburgh Handedness Inventory68.00(61.09)84.52(34.54)1.6775.7730.100
Age, years32.80(11.58)34.2(11.66)0.611030.543
Estimated verbal IQa104.30(8.42)b106.91(8.76)1.541030.127
HAM-D-173.98(4.04)–
HAM-A4.98(6.35)–
Y-BOCS total24.50(5.51)–
Y-BOCS obsessions12.06(3.57)–
Y-BOCS compulsions12.44(2.72)–
Onset, years20.96(8.28)–
Illness duration, years11.83(10.41)–
Stanford Sleepiness Scale3.42(1.59)3.31(1.43)−0.261020.793
IGT total net score4.00(27.53)11.09(23.43)2.311030.023*

Demographic and clinical characteristics of the participants.

HAM-A, Hamilton Anxiety Scale; HAM-D, Hamilton Depression Scale; Y-BOCS, Yale-Brown Obsessive Compulsive Scale; IGT, Iowa Gambling Task.

*

p < 0.05.

a

Verbal IQ was estimated by the Japanese version of National Adult Reading Test (JART).

b

One participant did not complete JART.

IGT Performance

The groups showed a significant difference in IGT performance (Table 1). The IGT total net score was significantly lower for patients with OCD than for CTL. After controlling for age, sex, and verbal IQ, this result remained unchanged.

To evaluate the effects of symptom severity on task performance of the OCD group, we also analyzed the association between IGT total net scores and Y-BOCS scores (total, obsessions, and compulsions), HAM-D scores, and HAM-A scores. We found no significant association between IGT performance and clinical scores.

fALFF Results

Associations Between IGT Performance and Slow-4 ALFFs

The groups showed a significant difference in the association between the IGT total net score and fALFFs in the left putamen (voxel height threshold of p < 0.001 and cluster size threshold of p < 0.05; FWE corrected) (Figures 1, 2 and Table 2). In the OCD group, greater fALFF values in the left putamen were correlated with lower IGT total net scores (r = −0.485; p < 0.0005). The CTL group showed an inverse pattern, however, with greater putamen fALFFs being correlated with higher IGT total net scores (r = 0.402; p < 0.005). In the OCD group, there were no significant associations between the clinical assessment (Y-BOCS, HAM-D, HAM-A) scores and putamen fALFFs.

Figure 1

Figure 2

Table 2

RegionKexyzp-FWEa
L. putamen72−24600.020839

Brain region where the association between IGT performance and slow-4 fALFFs was significantly different in OCD compared to CTL.

a

Cluster-level corrected p < 0.05 FWE after applying a voxel height threshold of p < 0.001.

Peak coordinates are given in MNI space.

L, left; R, right; Ke, Cluster extent; fALFFs, fractional amplitude of low-frequency fluctuations; IGT, Iowa Gambling Task.

In the supplemental analysis of covariance using a permutation-based cluster level correction, these results did not survive statistical significance.

Between-Group Differences in Slow-4 ALFFs

There were no significant differences between the slow-4 fALFFs in any brain region in the OCD and CTL groups.

Associations Between IGT Performance and Slow-5 ALFFs

There were no significant group differences in the association between slow-5 fALFFs and IGT performance.

Between-Group Differences in Slow-5 ALFFs

There were no significant differences between the slow-5 fALFFs in any brain region in the OCD and CTL groups.

Discussion

We conducted a neuroimaging study using fALFFs to identify traits associated with poor IGT performance in patients with OCD. IGT performance was significantly worse in the OCD patient group than in the control group. There were no significant associations between IGT performance and the severity of psychiatric symptoms in patients with OCD.

Our main finding was the inverse correlation between putamen slow-4 fALFFs and IGT performance in patients with OCD and CTL. This intriguing phenomenon can be attributed to the aberrant putamen function during the reinforcement learning process. After examining the brain activity of patients with OCD during a probabilistic reversal learning task, Hauser et al. (71) reported that the patients showed significantly increased reward prediction error (RPE) responses in the ACC and putamen compared to CTL. RPEs, which have a crucial role in reinforcement learning, have been shown to be encoded by the mesolimbic dopamine system including the striatum and ACC (72–77). The findings of Hauser et al. (71) suggest that the hypersensitization of this RPE encoding is responsible for impaired decision-making in patients with OCD. Moreira et al. (78) examined the brain activity of patients with OCD during a gambling task and found results similar to those of Hauser et al. (71): when patients with OCD perceived unexpected losses, they demonstrated larger deactivation in the ACC and putamen than CTL. Because negative RPEs generally cause deactivation of mesolimbic dopamine neurons (79), the findings of Moreira et al. (78) suggest hypersensitization of the RPE response with OCD.

Our data showed that among patients with OCD, higher putamen fALFFs at rest were correlated with worse IGT performances. In general, high putamen fALFFs at rest are predictive of large phasic reactions during a learning task; studies of healthy subjects have demonstrated that for each brain region, there is a fixed correlation pattern for spontaneous activity at rest and task-evoked activation (80–84), and that resting-state striatal fALFFs reflect dopamine release levels in the area (85, 86). Therefore, with OCD, higher putamen fALFFs may result in larger hypersensitive responses to RPE. Because IGT performance depends on the sensitivity to RPEs (especially the sensitivity to negative RPEs) (28), OCD patients with higher putamen fALFFs will perform poorly. In contrast, among control subjects without such hypersensitivity, higher putamen activity will contribute to appropriate learning through RPEs and improve IGT performance.

Nevertheless, it is possible that the putamen function itself is intact in patients with OCD and that abnormalities exist elsewhere. Although a rostral part of the putamen was detected during our study, the posterior putamen, caudate, and nucleus accumbens are primarily involved in RPE processing (77, 87, 88). The OFC, vmPFC, and dlPFC have important roles in the valuation of action outcomes and guidance of goal-directed behavior (28, 89, 90). The putamen receives the results of information processing in these regions and automates learned action sequences as a habit (91–94). Habit refers to the automatic and outcome-insensitive repetition of certain action sequences (95–97). Previous studies on decision-making deficits with OCD have inferred that aberrant information processing in the OFC and dlPFC, implicated in shaping goal-directed behavior, leads patients to make choices that do not produce valuable outcomes (98–102). Although our data showed no significant difference between the CTL and OCD groups in terms of resting-state fALFF values, the possibility of aberrations in the OFC or dlPFC cannot be ruled out. If information processing in these regions is altered, then disadvantageous behaviors will be learned and fixed as habits through putamen activity. Therefore, in patients with OCD, higher putamen activity may facilitate fixation of disadvantageous behaviors, which results in poorer IGT performance. On the contrary, among control subjects, information processing in the OFC and dlPFC is intact and leads to the appropriate selection of beneficial behaviors. Consequently, the higher putamen activity of CTL facilitates the fixation of beneficial behaviors, which results in better IGT performance. However, the present results could not confirm this. Future research using seed-based analysis to investigate the association between functional connectivity from the PFC to the putamen and decision-making ability should be conducted to verify this second explanation.

To our knowledge, the correlation between putamen fALFFs and IGT performance in healthy individuals has not been reported previously. Nevertheless, Kambeitz et al. (103) reported that healthy individuals with higher striatal dopamine levels at rest had higher net IGT scores. Because putamen fALFF values are thought to reflect the actual dopamine level in the region (85, 86, 104), our results are consistent with those reported by Kambeitz et al. (103). Additionally, according to Kayser et al. (56) and Smith et al. (53), healthy individuals with lower putamen activity and those with lower dopamine synthesis capacity in the putamen showed higher choice impulsivity and preferred immediate rewards to long-term rewards. Therefore, our CTL group findings are supported by these studies.

In the present study, we hypothesized that the abnormalities responsible for poor IGT performance with OCD would be found in the dorsal CSTC loop with projections from the dlPFC to the thalamus via the dorsal striatum. However, contrary to our hypothesis, there were no significant differences in fALFFs in any brain region of the OCD and CTL groups. Previous studies that recruited a large number of unmedicated patients with OCD (45, 49) showed higher fALFFs in the dlPFC and putamen in the patient group than in the control group. This inconsistency might be attributable to differences in the various profiles of the examined OCD patient groups. Functional neuroimaging studies of patients with OCD showed that aberrant activation of the dlPFC and putamen observed in patient groups was associated with the duration of illness (105, 106). Increased putamen activation was also related to greater comorbidity with mood and anxiety disorders (105). Furthermore, Mataix-Cols et al. (107) suggested that abnormal activation of the dlPFC and putamen with OCD is specifically associated with the aggressive/checking symptom dimension. By considering and comparing these factors in the present study and in the studies by Yang et al. (49) and Gao et al. (45), it is apparent that the illness duration was longer in our patient group (11.83 ± 10.41 years) than in the patient group studied by Yang et al. (6.4 ± 5.2 years; Gao et al. did not report this information). Regarding comorbidity with mood and anxiety disorders, our patients had even lower HAM-D and HAM-A scores (3.98 ± 4.04 and 4.98 ± 6.35, respectively) than the patients studied by Gao et al. (45) (6.7 ± 4.2 and 7.8 ± 5.4, respectively) and Yang et al. (49) (7.56 ± 3.76 and 7.6 ± 3.5, respectively), thus indicating that our patients more accurately represent the core OCD group without comorbidities. Although we cannot compare symptom dimensions because of the lack of information, it is possible that the studies by Gao et al. (45) and Yang et al. (49) included more patients with aggressive/checking symptoms than our study included. All these differences in the profiles of patients with OCD might be responsible for the inconsistent results.

The aforementioned factors are also associated with the limitations of this study. First, the different symptom dimensions of OCD may have distinct neural correlates (106–108). For a more precise understanding of decision-making deficits with OCD, a large number of patients with each symptom dimension should be enrolled in a study to compare IGT performance between the groups.

Second, the present study was not free from medication effects. Although we recruited 50 patients with OCD who were medication-free for at least 4 weeks, only 16 of them were drug-naïve. For the remaining patients, the possible residual effects of previous medications could not be ruled out. Therefore, more drug-naïve patients should be recruited in future analyses.

Third, we recruited only patients who were diagnosed with OCD without any other Axis I disorder. However, OCD is highly comorbid with anxiety and depressive disorders (109). It is unclear whether our findings are valid for patients with such comorbidities. Future work should include OCD patients with comorbidities and compare their results with the results of a core OCD group and a group of CTL to evaluate decision-making deficits.

Fourth, it is also unclear to what extent our findings are specific to OCD. Poor IGT performance has been reported in several psychiatric disorders (32–34). To confirm that our findings are strictly specific to OCD, we should recruit patients with these psychiatric disorders and examine group differences in fALFFs associated with the IGT scores.

Fifth, as control subjects, we recruited individuals who had no past and current DSM disorder. However, according to Kessler et al. (108), about half of the general population will meet the criteria for a DSM disorder at some point in life. Given such a high potential risk, it would be desirable to perform detailed clinical assessments of control subjects and compare the results with those of the patient group.

Lastly, it is possible that our results may be affected by false-positive risk (70). When we applied the most conservative statistical threshold to control for multiple comparisons (using a non-parametric permutation approach, with a voxel height threshold of p < 0.001 and cluster-size threshold of p < 0.05 FWE corrected) (70, 109), our results could not survive statistical significance. We consider that the presently applied threshold (using the typical SPM based approach with Gaussian random field theory, with cluster defined threshold with a voxel height threshold of p < 0.001 and cluster-level threshold of p < 0.05 FWE corrected) is strict enough to control FWE, even when we take into account the problem raised by Eklund et al. (70). Many recent neuroimaging studies (110, 111) also used a similar threshold for multiple comparison correction. Nevertheless, the possibility of false-positive risk cannot be completely ruled out.

Conclusions

We confirmed that unmedicated patients with OCD exhibited worse IGT performance than CTL. This poor performance was not correlated with the severity of clinical manifestations as assessed by the Y-BOCS or HAM-D. Furthermore, we examined group differences in fALFFs associated with the IGT scores of OCD patients and CTL. We found an inverse correlation between putamen slow-4 fALFFs and IGT performance in patients with OCD and CTL. Higher putamen fALFFs were correlated with lower IGT scores in patients with OCD and higher IGT scores in CTL. These findings highlight the importance of resting-state putamen activity for the processes of learning and decision-making as assessed by IGT. The inverse association detected in the present study is consistent with previously reported hypersensitive responses of the putamen to RPEs. Such abnormal functioning of the putamen may comprise a biological feature underlying aberrations in the CSTC loops that were observed during previous studies using task-related MRI to evaluate IGT performance in patients with OCD. If the perseveration tendency in OCD as assessed by IGT is related to the putamen function, the putamen as well as the network between the putamen and the prefrontal cortices may be targets for neuromodulatory treatments in OCD.

Funding

This work was supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI Grant Numbers (C) JP21K07547 and JP19K08076.

Publisher's Note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Statements

Data availability statement

The original contributions presented in the study are included in the article/supplementary material, further inquiries can be directed to the corresponding author/s.

Ethics statement

The studies involving human participants were reviewed and approved by Kyushu University Ethics Committee. The patients/participants provided their written informed consent to participate in this study.

Author contributions

SH designed the study, collected data, and wrote the initial draft of the manuscript. HT and KM collected data, designed the study, and critically reviewed the manuscript. AO and MK contributed to analysis and interpretation of data. TM, KKa, AM, KKi, and OT contributed to data collection. TN critically reviewed the manuscript. All authors approved the final version of the manuscript.

Acknowledgments

We thank the Japan Society for the Promotion of Science (JSPS) KAKENHI for advice regarding the analysis of imaging data.

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Summary

Keywords

obsessive-compulsive disorder (OCD), Iowa Gambling Task (IGT), decision-making, putamen, resting-state functional MRI, fractional amplitude of low-frequency fluctuations (fALFF)

Citation

Hasuzawa S, Tomiyama H, Murayama K, Ohno A, Kang M, Mizobe T, Kato K, Matsuo A, Kikuchi K, Togao O and Nakao T (2022) Inverse Association Between Resting-State Putamen Activity and Iowa Gambling Task Performance in Patients With Obsessive-Compulsive Disorder and Control Subjects. Front. Psychiatry 13:836965. doi: 10.3389/fpsyt.2022.836965

Received

16 December 2021

Accepted

21 April 2022

Published

13 May 2022

Volume

13 - 2022

Edited by

Taekwan Kim, Korea Advanced Institute of Science and Technology, South Korea

Reviewed by

Nathaniel Harnett, McLean Hospital, United States; Emily Belleau, McLean Hospital, United States; Junhee Lee, Eulji University, South Korea

Updates

Copyright

*Correspondence: Tomohiro Nakao

This article was submitted to Anxiety and Stress Disorders, a section of the journal Frontiers in Psychiatry

Disclaimer

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

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