Original Research ARTICLE
Evaluation of different genetic targets for Salmonella enterica serovar Enteriditis and Typhimurium, using Loop-mediated isothermal AMPlification for detection in food samples
- 1Life Sciences, Laboratório Ibérico Internacional de Nanotecnologia (INL), Portugal
Salmonella Enteritidis and Salmonella Typhimurium continue to be the most frequently identified serovars among confirmed cases of salmonellosis. In the current study different genetic targets (safA, sdf I, STM4497 and typh) were compared, attending to their specificity and sensitivity in pure cultures and in spiked samples, in order to determine their capacity to accurately identify them by loop-mediated isothermal amplification (LAMP). For the genes selected to detect Enteritidis, both performed equally well regarding their specificity, but safA proved more sensitive than Sdf I; minor differences were observed among these genes when analyzing spiked food samples. Regarding the targets for Typhimurium, STM4497 and typh, the former demonstrated to be more specific and sensitive, both when analyzing pure cultures as well as spiked samples. These results highlight the importance of an adequate evaluation of the genetic targets selected, before their implementation for routine analyses.
Keywords: Salmonella enteritidis, SAFA, Sdf I, Salmonella typhimurium, STM4497, typh, LAMP, Characterization methods
Received: 14 Dec 2017;
Accepted: 05 Feb 2018.
Edited by:Joshua B. Gurtler, Agricultural Research Service (USDA), United States
Reviewed by:Ben D. Tall, US FDA, United States
Tam L. Mai, IEH laboratories and consultant group/Molecular Epidemiology, United States
Junia Jean-Gilles Beaubrun, United States Food and Drug Administration, United States
Copyright: © 2018 Azinheiro, Carvalho, Prado and Garrido-Maestu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Dr. Alejandro Garrido-Maestu, Laboratório Ibérico Internacional de Nanotecnologia (INL), Life Sciences, Av. Mestre José Veiga s/n, Braga, 4715-330, Portugal, firstname.lastname@example.org