Deep Spatial Sequencing Revealing Differential Immune Responses in Human

Yan-Ping Yu¹Silvia Liu¹Caroline Obert²Bao-Guo Ren¹Marielle Krivet²Kyle Metcalfe²Jia-Jun Liu¹Tuval Ben-Yeheskel²Jianhua Luo^1*

• ¹University of Pittsburgh, Pittsburgh, United States
• ²Element Biosciences, INC, SAN DIEGO, United States

Hepatocellular carcinoma (HCC) is one of the most lethal cancers for humans. HCC is highly heterogeneous. In this study, we performed ultra-depth (~1 million reads per spot) sequencing of 6320 spatial transcriptomes on a case of HCC. Sixteen distinct spatial expression clusters were identified. Each of these clusters was spatially contiguous and had distinct gene expression patterns. In contrast, benign liver tissues showed minimal heterogeneity in terms of gene expression. Numerous immune cell-enriched spots were identified in both HCC and benign liver regions. Cells adjacent to these immune cell-enriched spots showed significant alterations in their gene expression patterns. Interestingly, the responses of HCC cells to the nearby immune cells were significantly more intense and broader, while the responses of benign liver cells to immune cells were somewhat narrow and muted, suggesting an innate difference in immune cell activities towards HCC cells in comparison with benign liver cells. However, cell-cell interaction analyses showed significant immune evasion by HCC cancer cells. When standard-depth sequencing was performed, significant numbers of genes and pathways that were associated with these changes disappeared. Qualitative differences in some pathways were also found. These results suggest that deep spatial sequencing may help to uncover previously unidentified mechanisms of liver cancer development.