Abstract
Some bacterial toxins and viruses have evolved the capacity to bind mammalian glycosphingolipids to gain access to the cell interior, where they can co-opt the endogenous mechanisms of cellular trafficking and protein translocation machinery to cause toxicity. Cholera toxin (CT) is one of the best-studied examples, and is the virulence factor responsible for massive secretory diarrhea seen in cholera. CT enters host cells by binding to monosialotetrahexosylganglioside (GM1 gangliosides) at the plasma membrane where it is transported retrograde through the trans-Golgi network (TGN) into the endoplasmic reticulum (ER). In the ER, a portion of CT, the CT-A1 polypeptide, is unfolded and then “retro-translocated” to the cytosol by hijacking components of the ER associated degradation pathway (ERAD) for misfolded proteins. CT-A1 rapidly refolds in the cytosol, thus avoiding degradation by the proteasome and inducing toxicity. Here, we highlight recent advances in our understanding of how the bacterial AB5 toxins induce disease. We highlight the molecular mechanisms by which these toxins use glycosphingolipid to traffic within cells, with special attention to how the cell senses and sorts the lipid receptors. We also discuss several new studies that address the mechanisms of toxin unfolding in the ER and the mechanisms of CT A1-chain retro-translocation to the cytosol.
Introduction
Bacterial toxins must access their cytosolic targets by translocating across a cell membrane (Inoue et al., 2011). This is no small feat, as the toxins are secreted as fully folded water-soluble proteins that undergo a series of structural changes allowing them to integrate into lipid membranes and inject the enzymatic domain in unfolded conformations across a cellular membrane to the cytosol. Once in the cytosol, this domain refolds into a functional enzyme that attacks specific cellular systems.
Cholera toxin (CT) typifies the AB5-subunit toxins. It is secreted by the Vibrio cholerae in the intestinal lumen. The toxin enters the cytosol of polarized epithelial cells lining the intestinal lumen by binding to the ganglioside GM1 via its B-subunit. GM1 follows a lipid-based sorting pathway to move the toxin retrograde through the trans-Golgi network (TGN) into the ER. In the ER, the enzymatically active portion of the A-subunit, termed the A1-chain, co-opts the machinery located in the ER lumen to manage terminally misfolded proteins in the secretory pathway (termed the ER associated degradation pathway or ERAD), which unfolds and retro-translocates the A1-chain to the cytosol. Unlike most retro-translocation substrates, the A1-chain escapes degradation by the proteasome and rapidly refolds in the cytosol to act as an ADP-ribosyltransferase. Toxicity is caused by ADP-ribosylation of the heterotrimeric G protein, Gsα that leads to constant activation of adenylyl cyclase and strong increases in cAMP intracellular levels. In intestinal cells, the rapid production of cAMP leads to intestinal chloride secretion, which causes a massive loss of water resulting in the rice-water secretory diarrhea that typifies cholera. This review will summarize current ideas and new findings for how glycosphingolipid-binding toxins gain access to the ER and the mechanisms involved in their retro-translocation into the cytosol (Figure 1).
Figure 1
Membrane trafficking of toxins and their lipid receptors
After binding to the ganglioside GM1 located on the outer leaflet of the cell membrane, CT travels through a complex endocytic pathway involving retrograde membrane traffic through the Golgi complex to the ER (Step 1) (Chinnapen et al., 2007). Other toxins, such as Shiga toxins (STx), Escherichia coli heat labile toxins and tetanus toxins and some viruses (SV40, polyomavirus) also use glycosphingolipids (gangliosides and globosides) as receptors to enter the cell and cause disease (Spooner et al., 2006). Exactly how the cell senses these lipids to sort them to different intracellular pathways is still unclear. Our knowledge of the endocytic and intracellular pathways co-opted by toxins and their lipid receptors has so far relied on microscopy observations of the bulk flow of fluorescently or radioactively labeled toxins and their lipid receptors or lipid analogues or lipid-specific antibodies. The lack of data tracking individual toxin or lipid molecules as they move within the cell, as well as the promiscuity of endocytic and intracellular pathways co-opted by them, has significantly hindered our ability to dissect the actual itinerary followed by these molecules from the cell surface to their sites of action.
The internalization of globotriaosylceramide (GB3) and GM1 glycosphingolipids, assessed using toxin or antibody markers, appears to be via clathrin-independent pathways, mainly through caveolae (Crespo et al., 2008), but clathrin-dependent pathways have also been reported (Step 2) (Torgersen et al., 2001). The glycosphingolipids seem to be required for the maintenance of caveolae domains (Singh et al., 2010). Gangliosides are well known to establish dynamic physicochemical interactions with cholesterol and other sphingolipids to form membrane nanodomains (Simons and Gerl, 2010; Sonnino and Prinetti, 2010). Exactly how the cell senses and sorts these domains into defined endocytic routes is still unknown. Recent studies have shown that STx, CT and SV40 virus can bind and crosslink glycosphingolipids to trigger membrane deformations (“tubules”) of both artificial membrane models and the plasma membrane of intact cells (Romer et al., 2007; Ewers et al., 2010; Romer et al., 2010). These tubular invaginations may favor toxin and virus internalization, although the capacity of these structures in driving toxin or virus entry is not yet defined. Tubular invaginations have been previously observed in the absence of ganglioside crosslinking, suggesting that other mechanisms of membrane deformation must exist as well (Massol et al., 2005; Boucrot et al., 2010). Although the mechanism for inducing membrane tubulation by the toxins and viruses is not clear, it appears that crosslinking of long chain gangliosides with the proper molecular spacing is required to induce membrane curvature (Ewers et al., 2010). Fission of tubules requires actin polymerization and dynamin function. Similarly to GB3 that binds STx, GM1 lipids containing long saturated acyl chains favor SV40 internalization and infection. Studies of internalization of GPI-anchored proteins, which also favor partitioning in lipid-microdomains or rafts, show that the molecular structures of the lipid chains affect the way they are internalized and trafficked in the cells (Bhagatji et al., 2009). The emerging theme from these studies is that clustering of gangliosides into lipid microdomains at the cell surface can initiate membrane budding events.
Although the preferential route of entry for these toxins and viruses is still quite controversial, it is likely that the actual entry mechanism has little impact in the intracellular sorting required to transport them to their final destinations. This is due to the convergence at the level of the early endosome of cargo derived from clathrin-dependent and clathrin-independent endocytic vesicles (Jovic et al., 2010). After internalization, ganglioside-antibody complexes are transported to the early endosome and later on accumulate transiently in Rab11-positive recycling endosomes en route to the plasma membrane (Step 3b) (Iglesias-Bartolome et al., 2006, 2009). A small fraction of ganglioside-antibody complexes is eventually transported to the Golgi complex and the endoplasmic reticulum (ER) (Step 3a) or to lysosomes.
Though the exact molecular requirements and intracellular location for sorting of the glycosphingolipids remain to be elucidated, it is likely that a key-sorting event takes place at the level of the early endosome. The early endosome exhibits a complex morphology with very thin (∼60 nm) tubular structures emanating from a main vesicular body thought to be functionally important, and many membrane traffic regulators (notably Rab5, Rab4, and their effectors) act on this organelle. Proteins and lipids targeted for recycling are driven into the tubular domains and eventually arrive back at the cell surface, while dissociated ligands remain in the vesicular region that matures into a late endosome/lysosome. Whereas the “geometry-based” model of lipid sorting proposed by Maxfield and McGraw (2004) explains several aspects of protein and ligand sorting at the early endosome, lipid sorting is likely more complex. Recent biophysical studies in model systems and live-cell imaging of lipid analogues have shown that lipid sorting likely depends on the coupling between membrane composition and high curvature of the tubular carriers (Andes-Koback and Keating, 2011); and that this is amplified in the presence of lipid-clustering proteins such as CT (Tian and Baumgart, 2009; Tian et al., 2009). Some toxins and viruses bound to gangliosides are further delivered to the Golgi and then to the ER but the mechanisms of sorting into the retrograde pathways remain unclear. Several protein complexes have been identified to be required for retrograde transport of STx, CT, and Ricin from the early endosome to the TGN/ER e.g., SNARE proteins (Syntaxins 5, 6, and 16) (Amessou et al., 2007; Ganley et al., 2008) and the retromer complex (Popoff et al., 2007, 2009). If, or how, these proteins interact with lipids to regulate sorting between intracellular trafficking pathways remains unknown. The lipid-raft associated protein Flotillin, implicated for clathrin-independent endocytic uptake of CT, has also been shown to be involved in the sorting of CT from the early endosome to the TGN (Glebov et al., 2006; Frick et al., 2007) and the ER (Saslowsky et al., 2010). The retrograde pathway to the ER may also involve a sorting step linking the early endosome to the ER without intersecting the TGN (Saslowsky et al., 2010). Another level of potential regulation of ganglioside and toxin sorting stems from the discovery of enzymes involved in ganglioside remodeling (Daniotti and Iglesias-Bartolome, 2011). Future studies will be needed to clarify the nature of lipid sorting at endosomes and the Golgi complex and the precise role of lipid remodeling in this process.
Mechanism of retro-translocation of toxins
Upon entering the ER, most AB5 bacterial toxins, including CT, undergo dissociation of their enzymatic moieties from their receptor binding subunits. The disulfide bonds that connect the A1 with the A2 polypeptide of CT are reduced in the ER followed by chaperone-mediated unfolding of the A1-chain. These two processing steps are required for the eventual retro-translocation of CT-A1 to the cytosol. The molecular details of these processes remain still unclear, but we favor the following model of CT-A1 dissociation. First, the ER lumenal chaperone protein disulfide isomerase (PDI) binds in its reduced state to a hydrophobic region at the C-terminus of CT-A1 chain allowing for unfolding and dissociation from the B-subunit (Step 4) (Tsai et al., 2001; Tsai and Rapoport, 2002). It is possible that PDI assists predominantly in the dissociation reaction and the CT-A1 subunit unfolds spontaneously (Pande et al., 2007; Massey et al., 2009; Banerjee et al., 2010; Taylor et al., 2011). A similar mechanism of toxin dissociation seems to operate for the AB toxin ricin and STx (Lord et al., 2003; Spooner et al., 2004; Fagerquist and Sultan, 2010).
Several other ER chaperones and components have been described to be of importance for CT retro-translocation to the cytosol (Step 5). The Hsp70 chaperone BiP (heavy chain binding protein) allows for proper folding of nascent peptide chains in the secretory pathway and also regulates the retro-translocation of several ERAD substrates (Nishikawa et al., 2005). In vitro studies suggest that BiP plays a role in maintaining the A1-chain in a soluble, export-competent state required for the retro-translocation reaction (Winkeler et al., 2003), and intoxication by CT may actually up-regulate expression of BiP (Dixit et al., 2008). There is also evidence that the unfolded form of CT-A1 interacts with the ER luminal chaperone Hsp40, ERdj3/HEDJ, in order to mask solvent-exposed hydrophobic residues during retro-translocation (Massey et al., 2011). Interestingly, ERdj3 was shown to directly interact with BiP in the ER (Shen and Hendershot, 2005), suggesting an ERdj3/BiP complex may be involved in stabilizing the unfolded CT-A1 and later targeting it to the retro-translocation channels. ERdj3/HEDJ and BiP have also been shown to interact with the A-subunit of STx and to be important in retro-translocation of the STx enzymatic A-subunit (Yu and Haslam, 2005; Falguieres and Johannes, 2006). In the case of ricin, the ER degradation enhancing alpha-mannosidase I-like protein (EDEM) was reported to facilitate its targeting to the retro-translocons (Slominska-Wojewodzka et al., 2006).
Once unfolded, the CT-A1 chain is presumably targeted to the ER membrane for retro-translocation through a protein-conducting channel. The molecular identity of this channel remains unknown. There is some evidence that the CT-A1 chain may retro-translocate through the Sec61 complex as also proposed for ricin (Rapak et al., 1997) and STx (Yu and Haslam, 2005), but whether, and how, this may occur remains unclear (Wiertz et al., 1996; Pilon et al., 1997; Matlack et al., 1998; Schmitz et al., 2000). Other potential candidates for the retro-translocation channel have emerged, with greatest evidence for a protein complex centered on the E3 ubiquitin ligases HRD1 and gp78 (Bernardi et al., 2010). Other proteins of this complex and adaptor proteins include UBE2G2, SEL1L, AUP1, OS-9, XTP3-B, and Derlins (Smith et al., 2011). Not all may be required for every reaction. Different toxins may utilize different components (Carvalho et al., 2010). In the case of CT, there is there is good evidence that the Hrd1/gp78 complex is required (Bernardi et al., 2010; Carvalho et al., 2010) but conflicting evidence for the dependence on Derlin (Bernardi et al., 2008; Dixit et al., 2008; Saslowsky et al., 2010).
The driving force behind CT retro-translocation and the identities of the cytosolic components in the retro-translocation process also remains to be determined (Step 6). We believe the cytosolic AAA-ATPase p97 that pulls many ERAD substrates from the ER membrane does not play a role in retro-translocation of the CT A1-chain (Kothe et al., 2005). It is possible that an ER luminal AAA-ATPase, TorsinA (Nery et al., 2011), may be involved, but exactly how TorsinA affects CT retro-translocation is not solved. Most studies show that ubiquitination of the toxins is not involved driving retro-translocation (Rodighiero et al., 2002; Li et al., 2010; Wernick et al., 2010).
It is now clear that once in the cytosol, the CT-A1 refolds into a stable conformation that is resistant to proteasomal degradation (Rodighiero et al., 2002). There is evidence this requires cytosolic chaperones. Refolding of the A1-chain in the cytosol depends in part on the small GTPase ADP-ribosylation factor 6 (ARF6), a cytosolic eukaryotic protein that can also enhance the enzymatic activity of CT-A1 (Teter et al., 2006; Pande et al., 2007; Ampapathi et al., 2008), and the cytosolic chaperone Hsp90 that may act to protect the A1-chain from degradation (Taylor et al., 2010) to induce toxicity (Step 7). Refolding of ricin in the cytosol is helped in part by the chaperone Hsc70 and Hsp90 (Spooner et al., 2008).
Summary
While many aspects of lipid-dependent trafficking and retro-translocation of the AB5 toxins remain to be discovered, the recent work in this field has established the outlines of how the toxins co-opt the host cell to enter the cytosol and cause disease. These topics address important aspects of cell biology and toxin action, with broad impact on our understanding of host-pathogen interactions in general.
Conflict of interest statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Statements
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
References
1
AmessouM.FradagradaA.FalguieresT.LordJ. M.SmithD. C.RobertsL. M.LamazeC.JohannesL. (2007). Syntaxin 16 and syntaxin 5 are required for efficient retrograde transport of several exogenous and endogenous cargo proteins. J. Cell Sci. 120, 1457–1468. 10.1242/jcs.03436
2
AmpapathiR. S.CreathA. L.LouD. I.CraftJ. W. Jr.BlankeS. R.LeggeG. B. (2008). Order-disorder-order transitions mediate the activation of cholera toxin. J. Mol. Biol. 377, 748–760. 10.1016/j.jmb.2007.12.075
3
Andes-KobackM.KeatingC. D. (2011). Complete budding and asymmetric division of primitive model cells to produce daughter vesicles with different interior and membrane compositions. J. Am. Chem. Soc. 133, 9545–9555. 10.1021/ja202406v
4
BanerjeeT.PandeA.JoblingM. G.TaylorM.MasseyS.HolmesR. K.TatulianS. A.TeterK. (2010). Contribution of subdomain structure to the thermal stability of the cholera toxin A1 subunit. Biochemistry49, 8839–8846. 10.1021/bi101201c
5
BernardiK. M.ForsterM. L.LencerW. I.TsaiB. (2008). Derlin-1 facilitates the retro-translocation of cholera toxin. Mol. Biol. Cell19, 877–884. 10.1091/mbc.E07-08-0755
6
BernardiK. M.WilliamsJ. M.KikkertM.van VoordenS.WiertzE. J.YeY.TsaiB. (2010). The E3 ubiquitin ligases Hrd1 and gp78 bind to and promote cholera toxin retro-translocation. Mol. Biol. Cell21, 140–151. 10.1091/mbc.E09-07-0586
7
BhagatjiP.LeventisR.ComeauJ.RefaeiM.SilviusJ. R. (2009). Steric and not structure-specific factors dictate the endocytic mechanism of glycosylphosphatidylinositol-anchored proteins. J. Cell Biol. 186, 615–628. 10.1083/jcb.200903102
8
BoucrotE.SaffarianS.ZhangR.KirchhausenT. (2010). Roles of AP-2 in clathrin-mediated endocytosis. PLoS One5:e10597. 10.1371/journal.pone.0010597
9
CarvalhoP.StanleyA. M.RapoportT. A. (2010). Retrotranslocation of a misfolded luminal ER protein by the ubiquitin-ligase Hrd1p. Cell143, 579–591. 10.1016/j.cell.2010.10.028
10
ChinnapenD. J.ChinnapenH.SaslowskyD.LencerW. I. (2007). Rafting with cholera toxin: endocytosis and trafficking from plasma membrane to ER. FEMS Microbiol. Lett. 266, 129–137. 10.1111/j.1574-6968.2006.00545.x
11
CrespoP. M.von MuhlinenN.Iglesias-BartolomeR.DaniottiJ. L. (2008). Complex gangliosides are apically sorted in polarized MDCK cells and internalized by clathrin-independent endocytosis. FEBS J. 275, 6043–6056. 10.1111/j.1742-4658.2008.06732.x
12
DaniottiJ. L.Iglesias-BartolomeR. (2011). Metabolic pathways and intracellular trafficking of gangliosides. IUBMB Life63, 513–520. 10.1002/iub.477
13
DixitG.MikoryakC.HayslettT.BhatA.DraperR. K. (2008). Cholera toxin up-regulates endoplasmic reticulum proteins that correlate with sensitivity to the toxin. Exp. Biol. Med. (Maywood)233, 163–175. 10.3181/0705-RM-132
14
EwersH.RomerW.SmithA. E.BaciaK.DmitrieffS.ChaiW.ManciniR.KartenbeckJ.ChambonV.BerlandL.OppenheimA.SchwarzmannG.FeiziT.SchwilleP.SensP.HeleniusA.JohannesL. (2010). GM1 structure determines SV40-induced membrane invagination and infection. Nat. Cell Biol. 12, 11–18. (Suppl. 11–12). 10.1038/ncb1999
15
FagerquistC. K.SultanO. (2010). Top-down proteomic identification of furin-cleaved alpha-subunit of Shiga toxin 2 from Escherichia coli O157:H7 using MALDI-TOF-TOF-MS/MS. J. Biomed. Biotechnol. 2010, 123460. 10.1039/c0an00909a
16
FalguieresT.JohannesL. (2006). Shiga toxin B-subunit binds to the chaperone BiP and the nucleolar protein B23. Biol. Cell98, 125–134. 10.1042/BC20050001
17
FrickC. G.RichtsfeldM.SahaniN. D.KanekiM.BlobnerM.MartynJ. A. (2007). Long-term effects of botulinum toxin on neuromuscular function. Anesthesiology106, 1139–1146. 10.1097/01.anes.0000267597.65120.16
18
GanleyI. G.EspinosaE.PfefferS. R. (2008). A syntaxin 10-SNARE complex distinguishes two distinct transport routes from endosomes to the trans-Golgi in human cells. J. Cell Biol. 180, 159–172. 10.1083/jcb.200707136
19
GlebovO. O.BrightN. A.NicholsB. J. (2006). Flotillin-1 defines a clathrin-independent endocytic pathway in mammalian cells. Nat. Cell Biol. 8, 46–54. 10.1038/ncb1342
20
Iglesias-BartolomeR.CrespoP. M.GomezG. A.DaniottiJ. L. (2006). The antibody to GD3 ganglioside, R24, is rapidly endocytosed and recycled to the plasma membrane via the endocytic recycling compartment. Inhibitory effect of brefeldin A and monensin.FEBS J. 273, 1744–1758. 10.1111/j.1742-4658.2006.05194.x
21
Iglesias-BartolomeR.TrenchiA.CominR.MoyanoA. L.NoresG. A.DaniottiJ. L. (2009). Differential endocytic trafficking of neuropathy-associated antibodies to GM1 ganglioside and cholera toxin in epithelial and neural cells. Biochim. Biophys. Acta1788, 2526–2540. 10.1016/j.bbamem.2009.09.018
22
InoueT.MooreP.TsaiB. (2011). How viruses and toxins disassemble to enter host cells. Annu. Rev. Microbiol. 65, 287–305. 10.1146/annurev-micro-090110-102855
23
JovicM.SharmaM.RahajengJ.CaplanS. (2010). The early endosome: a busy sorting station for proteins at the crossroads. Histol. Histopathol. 25, 99–112.
24
KotheM.YeY.WagnerJ. S.De LucaH. E.KernE.RapoportT. A.LencerW. I. (2005). Role of p97 AAA-ATPase in the retrotranslocation of the cholera toxin A1 chain, a non-ubiquitinated substrate. J. Biol. Chem. 280, 28127–28132. 10.1074/jbc.M503138200
25
LiS.SpoonerR. A.AllenS. C.GuiseC. P.LaddsG.SchnoderT.SchmittM. J.LordJ. M.RobertsL. M. (2010). Folding-competent and folding-defective forms of ricin A chain have different fates after retrotranslocation from the endoplasmic reticulum. Mol. Biol. Cell21, 2543–2554. 10.1091/mbc.E09-08-0743
26
LordM. J.JolliffeN. A.MarsdenC. J.PatemanC. S.SmithD. C.SpoonerR. A.WatsonP. D.RobertsL. M. (2003). Ricin. Mechanisms of cytotoxicity. Toxicol. Rev22, 53–64.
27
MasseyS.BanerjeeT.PandeA. H.TaylorM.TatulianS. A.TeterK. (2009). Stabilization of the tertiary structure of the cholera toxin A1 subunit inhibits toxin dislocation and cellular intoxication. J. Mol. Biol. 393, 1083–1096. 10.1016/j.jmb.2009.09.013
28
MasseyS.BurressH.TaylorM.NemecK. N.RayS.HaslamD. B.TeterK. (2011). Structural and functional interactions between the cholera toxin A1 subunit and ERdj3/HEDJ, a chaperone of the endoplasmic reticulum. Infect. Immun. 79, 4739–4747. 10.1128/IAI.05503-11
29
MassolR. H.LarsenJ. E.KirchhausenT. (2005). Possible role of deep tubular invaginations of the plasma membrane in MHC-I trafficking. Exp. Cell Res. 306, 142–149. 10.1016/j.yexcr.2005.01.021
30
MatlackK. E.MothesW.RapoportT. A. (1998). Protein translocation: tunnel vision. Cell92, 381–390. 10.1016/S0092-8674(00)80930-7
31
MaxfieldF. R.McGrawT. E. (2004). Endocytic recycling. Nat. Rev. Mol. Cell Biol. 4, 121–132. 10.1038/nrm1315
32
NeryF. C.ArmataI. A.FarleyJ. E.ChoJ. A.YaqubU.ChenP.Da HoraC. C.WangQ.TagayaM.KleinC.TannousB.CaldwellK. A.CaldwellG. A.LencerW. I.YeY.BreakefieldX. O. (2011). TorsinA participates in endoplasmic reticulum-associated degradation. Nat. Commun. 2, 393. 10.1038/ncomms1383
33
NishikawaS.BrodskyJ. L.NakatsukasaK. (2005). Roles of molecular chaperones in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). J. Biochem. 137, 551–555. 10.1093/jb/mvi068
34
PandeA. H.ScaglioneP.TaylorM.NemecK. N.TuthillS.MoeD.HolmesR. K.TatulianS. A.TeterK. (2007). Conformational instability of the cholera toxin A1 polypeptide. J. Mol. Biol. 374, 1114–1128. 10.1016/j.jmb.2007.10.025
35
PilonM.SchekmanR.RomischK. (1997). Sec61p mediates export of a misfolded secretory protein from the nedoplasmic reticulum to the cytososl for degradation. EMBO J. 16, 4540–4548. 10.1093/emboj/16.15.4540
36
PopoffV.MardonesG. A.BaiS. K.ChambonV.TenzaD.BurgosP. V.ShiA.BenarochP.UrbeS.LamazeC.GrantB. D.RaposoG.JohannesL. (2009). Analysis of articulation between clathrin and retromer in retrograde sorting on early endosomes. Traffic10, 1868–1880. 10.1111/j.1600-0854.2009.00993.x
37
PopoffV.MardonesG. A.TenzaD.RojasR.LamazeC.BonifacinoJ. S.RaposoG.JohannesL. (2007). The retromer complex and clathrin define an early endosomal retrograde exit site. J. Cell Sci. 120, 2022–2031. 10.1242/jcs.003020
38
RapakA.FalnesP. Ø.OlsnesS. (1997). Retrograde transport of mutant ricin to the endoplasmic reticulum with subsequent translocation to the cytosol. Proc. Natl. Acad. Sci. U.S.A. 94, 3783–3788.
39
RodighieroC.TsaiB.RapoportT. A.LencerW. I. (2002). Role of ubiquitination in retro-translocation of cholera toxin and escape of cytosolic degradation. EMBO Rep. 3, 1222–1227. 10.1093/embo-reports/kvf239
40
RomerW.BerlandL.ChambonV.GausK.WindschieglB.TenzaD.AlyM. R.FraisierV.FlorentJ. C.PerraisD.LamazeC.RaposoG.SteinemC.SensP.BassereauP.JohannesL. (2007). Shiga toxin induces tubular membrane invaginations for its uptake into cells. Nature450, 670–675. 10.1038/nature05996
41
RomerW.PontaniL. L.SorreB.RenteroC.BerlandL.ChambonV.LamazeC.BassereauP.SykesC.GausK.JohannesL. (2010). Actin dynamics drive membrane reorganization and scission in clathrin-independent endocytosis. Cell140, 540–553. 10.1016/j.cell.2010.01.010
42
SaslowskyD. E.ChoJ. A.ChinnapenH.MassolR. H.ChinnapenD. J.WagnerJ. S.De LucaH. E.KamW.PawB. H.LencerW. I. (2010). Intoxication of zebrafish and mammalian cells by cholera toxin depends on the flotillin/reggie proteins but not Derlin-1 or -2. J. Clin. Invest. 120, 4399–4409. 10.1172/JCI42958
43
SchmitzA.HerrgenH.WinkelerA.HerzogV. (2000). Cholera toxin is exported from microsomes by the sec61p complex. J. Cell Biol. 148, 1203–1212.
44
ShenY.HendershotL. M. (2005). ERdj3, a stress-inducible endoplasmic reticulum DnaJ homologue, serves as a cofactor for BiP's interactions with unfolded substrates. Mol. Biol. Cell16, 40–50. 10.1091/mbc.E04-05-0434
45
SimonsK.GerlM. J. (2010). Revitalizing membrane rafts: new tools and insights. Nat. Rev. Mol. Cell Biol. 11, 688–699. 10.1038/nrm2977
46
SinghR. D.MarksD. L.HolickyE. L.WheatleyC. L.KaptzanT.SatoS. B.KobayashiT.LingK.PaganoR. E. (2010). Gangliosides and beta1-integrin are required for caveolae and membrane domains. Traffic11, 348–360. 10.1111/j.1600-0854.2009.01022.x
47
Slominska-WojewodzkaM.GregersT. F.WalchliS.SandvigK. (2006). EDEM is involved in retrotranslocation of ricin from the endoplasmic reticulum to the cytosol. Mol. Biol. Cell17, 1664–1675. 10.1091/mbc.E05-10-0961
48
SmithM. H.PloeghH. L.WeissmanJ. S. (2011). Road to ruin: targeting proteins for degradation in the endoplasmic reticulum. Science334, 1086–1090. 10.1126/science.1209235
49
SonninoS.PrinettiA. (2010). Gangliosides as regulators of cell membrane organization and functions. Adv. Exp. Med. Biol. 688, 165–184.
50
SpoonerR. A.HartP. J.CookJ. P.PietroniP.RogonC.HohfeldJ.RobertsL. M.LordJ. M. (2008). Cytosolic chaperones influence the fate of a toxin dislocated from the endoplasmic reticulum. Proc. Natl. Acad. Sci. U.S.A. 105, 17408–17413. 10.1073/pnas.0809013105
51
SpoonerR. A.SmithD. C.EastonA. J.RobertsL. M.LordJ. M. (2006). Retrograde transport pathways utilised by viruses and protein toxins. Virol. J. 3, 26. 10.1186/1743-422X-3-26
52
SpoonerR. A.WatsonP. D.MarsdenC. J.SmithD. C.MooreK. A.CookJ. P.LordJ. M.RobertsL. M. (2004). Protein disulphide-isomerase reduces ricin to its A and B chains in the endoplasmic reticulum. Biochem. J. 383, 285–293. 10.1042/BJ20040742
53
TaylorM.BanerjeeT.RayS.TatulianS. A.TeterK. (2011). Protein-disulfide isomerase displaces the cholera toxin A1 subunit from the holotoxin without unfolding the A1 subunit. J. Biol. Chem. 286, 22090–22100. 10.1074/jbc.M111.237966
54
TaylorM.Navarro-GarciaF.HuertaJ.BurressH.MasseyS.IretonK.TeterK. (2010). Hsp90 is required for transfer of the cholera toxin A1 subunit from the endoplasmic reticulum to the cytosol. J. Biol. Chem. 285, 31261–31267. 10.1074/jbc.M110.148981
55
TeterK.JoblingM. G.SentzD.HolmesR. K. (2006). The cholera toxin A1(3) subdomain is essential for interaction with ADP-ribosylation factor 6 and full toxic activity but is not required for translocation from the endoplasmic reticulum to the cytosol. Infect. Immun. 74, 2259–2267. 10.1128/IAI.74.4.2259-2267.2006
56
TianA.BaumgartT. (2009). Sorting of lipids and proteins in membrane curvature gradients. Biophys. J. 96, 2676–2688. 10.1016/j.bpj.2008.11.067
57
TianA.CapraroB. R.EspositoC.BaumgartT. (2009). Bending stiffness depends on curvature of ternary lipid mixture tubular membranes. Biophys. J. 97, 1636–1646. 10.1016/j.bpj.2009.07.012
58
TorgersenM. L.SkrettingG.van DeursB.SandvigK. (2001). Internalization of cholera toxin by different endocytic mechanisms. J. Cell Sci. 114, 3737–3747.
59
TsaiB.RapoportT. (2002). Unfolded cholera toxin is transferred to the ER membrane and released from protein disulfide isomerase upon oxidation by Ero1. J. Cell Biol. 159, 207–215. 10.1083/jcb.200207120
60
TsaiB.RodighieroC.LencerW. I.RapoportT. (2001). Protein disulfide isomerase acts as a redox-dependent chaperone to unfold cholera toxin. Cell104, 937–948. 10.1016/S0092-8674(01)00289-6
61
WernickN. L.de LucaH.KamW. R.LencerW. I. (2010). N-terminal extension of the cholera toxin A1-chain causes rapid degradation after retrotranslocation from endoplasmic reticulum to cytosol. J. Biol. Chem. 285, 6145–6152. 10.1074/jbc.M109.062067
62
WiertzE. J.TortorellaD.BogyoM.YuJ.MothesW.JonesT. R.RapoportT. A.PloeghH. L. (1996). Sec61-mediated transfer of a membrane protein from the endoplasmic reticulum to the proteasome for destruction. Nature384, 432–438. 10.1038/384432a0
63
WinkelerA.GodderzD.HerzogV.SchmitzA. (2003). BiP-dependent export of cholera toxin from endoplasmic reticulum-derived microsomes. FEBS Lett. 554, 439–442.
64
YuM.HaslamD. B. (2005). Shiga toxin is transported from the endoplasmic reticulum following interaction with the luminal chaperone HEDJ/ERdj3. Infect. Immun. 73, 2524–2532. 10.1128/IAI.73.4.2524-2532.2005
Summary
Keywords
cholera toxin, membrane trafficking, retro-translocation, ERAD
Citation
Cho JA, Chinnapen DJ-F, Aamar E, te Welscher YM, Lencer WI and Massol R (2012) Insights on the trafficking and retro-translocation of glycosphingolipid-binding bacterial toxins. Front. Cell. Inf. Microbio. 2:51. doi: 10.3389/fcimb.2012.00051
Received
24 January 2012
Accepted
24 March 2012
Published
11 April 2012
Volume
2 - 2012
Edited by
Ken Bradley, University of California, Los Angeles, USA
Reviewed by
Vincent J. Starai, The University of Georgia, USA; Vernon L. Tesh, Texas A&M University Health Science Center, USA
Copyright
© 2012 Cho, Chinnapen, Aamar, te Welscher, Lencer and Massol.
This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.
*Correspondence: Ramiro Massol and Wayne I. Lencer, Division of Gastroenterology and Nutrition, Children's Hospital Boston, 300 Longwood Avenue, Enders Bldg. Rm. 609, Boston, MA 02115, USA. e-mail: ramiro.massol@childrens.harvard.edu; wayne.lencer@childrens.harvard.edu
Disclaimer
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.