Effect of PPD Inoculation on Serum Cytokine Profiles in Latent Tuberculosis Infection Populations

Wenjing Chang¹Dingyong Sun^1*Yanqiu Zhang¹Shaoping Ji²Shaohua Wang¹Weidong Wang¹Xiaoguang Ma¹Danwei Zheng¹Ruyue Su¹Yankun Zhu¹Jie Shi¹Linqi Diao^1*

• ¹Henan Center for Disease Control and Prevention, Zhengzhou, China
• ²Center for Molecular Medicine， Zhengzhou Health College, Zhengzhou, China

This study aims to investigate the impact of PPD skin test administration on serum cytokine levels in individuals with latent tuberculosis infection (LTBI) compared to healthy controls. Through quantitative analysis of multiple cytokines in serum samples before and after testing, significant differences were observed in cytokine level changes among LTBI individuals following PPD stimulation. The results indicate that prior to PPD administration, there were no significant differences in cytokine levels between the LTBI group and healthy controls. However, following PPD testing, individuals with LTBI exhibited significantly elevated levels of β-NGF, Eotaxin, G-CSF, GRO-α, IL-10, IL-17A, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-6, IP-10, LIF, and SDF-1α, while MCP-1 levels were significantly reduced compared to those in healthy controls. Comparative analysis of different population groups before and after PPD skin test administration revealed no significant changes in serum levels of SCGF-β, G-CSF, MCP-3, or IL-3 among healthy controls. In contrast, individuals with latent tuberculosis infection (LTBI) exhibited significantly elevated levels of G-CSF, MCP-3, and IL-3, while SCGF-β levels were markedly reduced. This study provides novel insights into the differential immune responses to PPD testing in latent tuberculosis infection and identifies potential biomarkers for early monitoring of immune activation in tuberculosis infection.