MINI REVIEW article

Front. Ecol. Evol., 06 January 2023

Sec. Evolutionary Ecology of Social Behavior

Volume 10 - 2022 | https://doi.org/10.3389/fevo.2022.1001045

What is really social about social insect cognition?

  • Research Research Center on Animal Cognition (CRCA), Center for Integrative Biology (CBI), CNRS-University Paul Sabatier, Toulouse, France

Abstract

It is often assumed that social life imposes specific cognitive demands for animals to communicate, cooperate and compete, ultimately requiring larger brains. The “social brain” hypothesis is supported by data in primates and some other vertebrates, but doubts have been raised over its applicability to other taxa, and in particular insects. Here, we review recent advances in insect cognition research and ask whether we can identify cognitive capacities that are specific to social species. One difficulty involved in testing the social brain hypothesis in insects is that many of the model species used in cognition studies are highly social (eusocial), and comparatively little work has been done in insects that live in less integrated social structures or that are solitary. As more species are studied, it is becoming clear that insects share a rich cognitive repertoire and that these abilities are not directly related to their level of social complexity. Moreover, some of the cognitive mechanisms involved in many social interactions may not differ from those involved in non-social behaviors. We discuss the need for a more comparative and neurobiologically grounded research agenda to better understand the evolution of insect brains and cognition.

1. Introduction

The considerable variation in brain size and cognitive abilities in the animal kingdom has been a long-standing question for biologists (Healy, 2021). It is broadly accepted that the brain evolved to process ecological information. However, it is an energetically expensive organ to operate and maintain. Therefore explanations for its evolution must reconcile these costs with fitness payoffs (Aiello and Wheeler, 1995). This presented an especially intriguing puzzle for primatologists who observed that the brains of primates were significantly larger than predicted, given their body size (Clutton-Brock and Harvey, 1980), and that classical explanations implicating their need for complex problem-solving (Gibson, 1986) did not account for these trends in all primate groups. The correlation between neocortex size and social group size in primates hinted at a link between brain size and sociality, later formalized as the “social brain hypothesis” (Dunbar, 1992; Dunbar, 1998). Following this hypothesis, increases in the frequency and complexity of social interactions, and the cognitive challenges of managing social relationships, may have required better information processing capabilities and larger brains. Over the past decades, research in primates has yielded experimental support for this hypothesis, reporting that neocortex size is also associated with intensity of reproductive competition (Lindenfors, 2005), frequency of coalition formation (Dunbar and Shultz, 2007), social play (Lewis, 2001), and social learning (Reader and Laland, 2002).

The story is a bit different in insects. While early description of insect anatomy and behavior also suggested a strong relationship between cognition and sociality (Dujardin, 1850), recent attempts to correlate brain size with metrics of social complexity are ambiguous (Riveros et al., 2012; O'Donnell et al., 2015; Farris, 2016; Kamhi et al., 2016; Gordon et al., 2019; Kamhi et al., 2019), emphasizing the need for more research (Lihoreau et al., 2012a; Simons and Tibbetts, 2019). Insects, like primates, display diverse social forms (Costa, 2006; Hölldobler and Wilson, 2009) which are accompanied by brain size variations (Strausfeld, 2012). However, the social organization of insect colonies can be dramatically different from that of vertebrates. In their most socially advanced forms (eusociality), insect colonies are characterized by extensive division of labor, cooperative brood rearing, and overlapping generations of adults (Batra, 1966; Michener, 1974; Wilson, 1975). This means that workers (i.e., the individuals on which most cognitive studies are performed) are sterile and fitness payoffs are experienced at the level of the group. Reproductive competition, mate selection and pair bonding - some of the primary drivers of brain evolution in social vertebrates (Healy, 2021) - therefore do not occur in these individuals. In fact, increases in insect social complexity are predicted to decrease overall brain size through obligate division of labor which allows for investment in functionally specialized brain regions (Gronenberg and Riveros, 2009; O'Donnell et al., 2015). In this way, the social structure of an insect colony may reduce the need for individual investment in neural tissue while improving overall performance in a few specialized tasks.

These recent theoretical developments, coupled with the observation that most of the impressive cognitive feats reported in insects have been identified in the context of individual foraging, and not social interactions (von Frisch, 1967; Giurfa, 2013; Wehner, 2020), draw attention to the emerging role of competition and foraging ecology in the evolution of insect cognition (Kamhi et al., 2016, 2019). For instance, a phylogenetic analysis has shown that the elaborated mushroom bodies in the insect brain (i.e., neuroanatomical structures involved in learning and memory), were acquired roughly 90MYR before the evolution of eusociality, coinciding with the switch from phytophagy to parasitoidism and the origin of central-place foraging (Farris and Schulmeister, 2011). This suggests the challenge of navigating and finding food in various types of landscapes drove the evolution of enhanced cognitive processes in what would later become eusocial insects (Jeanson and Weidenmüller, 2014; Farris, 2016). This observation thus raises the possibility that enhanced cognitive abilities may not be unique to just eusocial insects, but could be observed in any species that experiences the appropriate ecological conditions.

In this mini-review we consider whether many of the cognitive abilities once thought to be exclusive to social insects can evolve through non-social ecological processes. We argue that the primary drivers of insect cognition are processes related to reproductive competition and foraging. Using the extensive body of literature surrounding cognitive abilities in highly eusocial hymenopterans (i.e., honey bees, ants, and wasps) and the more recent, though rapidly expanding field of non-eusocial insect cognition (e.g., drosophila), we show that enhanced cognition abilities can evolve in other social structures (encompassing presocial, subsocial, semisocial, parasocial, quasisocial, facultatively social, cooperative breeding, gregarious, etc. species, sensuMichener, 1974; Wilson, 1975; Crespi and Yanega, 1995; Costa, 2006) and in solitary species.

2. Cognitive abilities shared by eusocial and non-eusocial insects

Advanced levels of sociality require cognitive abilities supporting precise social interactions (Byrne and Bates, 2007). For insects, this may mean being able to assess group identity, recognize specific individuals, remember past interactions with them, share information, find and learn the localization of food resources and navigate back to the colony nest (Wenseleers and van Zweden, 2017). Below we describe how many of these cognitive abilities, once thought to be unique to eusocial insects, have in fact recently been reported in some non-eusocial species and we discuss the possible explanations for how such traits could have evolved independently of sociality (see summary in Table 1).

Table 1

EusocialNon-eusocial
Individual recognition- Wasps establish reproductive dominance hierarchies based on facial coloration patterns (Tibbetts, 2002)- Fruit flies establish dominance hierarchies for mate competition (Yurkovic et al., 2006) (unknown mechanism)
- Ant queens establish reproductive dominance hierarchies based on chemical cues (D’Ettorre and Heinze, 2005)- Crickets select mating partners through self-referent odor phenotype matching (Capodeanu-Nägler et al., 2014)
Numerosity- Honey bees count landmarks (Chittka and Geiger, 1995), perform basic additions (Howard et al., 2019), and have the concept of zero (Howard et al., 2018)- Tenebrio beetles adjust mate guarding duration to the number of males encountered before mating (Carazo et al., 2009)
-Desert ants count steps to evaluate distances during path integration (Wittlinger et al., 2006)- Ladybugs adjust egg laying strategy according to the number of larvae or other females encountered (Hemptinne et al., 1992)
Non-elemental learning- Honey bees solve reversal learning and patterning tasks (Boitard et al., 2015; Devaud et al., 2015)- Fruit flies solve reversal learning (Mancini et al., 2019) and patterning tasks (Durrieu et al., 2020)
Social learning- Honey bees share information locational information about food sources (von Frisch, 1967) through the waggle dance- Fruit flies copy the choices of oviposition sites (Battesti et al., 2012) and mating partners (Danchin et al., 2018) through visual observation
- Bumblebee bees learn flower preferences (Leadbeater and Chittka, 2005; Worden and papaj, 2005) and new foraging techniques (Alem et al., 2016; Loukola et al., 2017) through visual observation
Tool use- Some ant species use soil debris to soak liquid food and carry it back to their nest (Maak et al., 2020)-Ant-lion larvae throw sand to make their prey fall into the bottom of their pit (Oguma, 1930)
- Several ant and wasp species close their nest using soil/stone deposits (Pierce, 1986)-African tree crickets use and manipulate leaves to amplify their calls (Prozesky-Schulze et al., 1975)
- Solitary wasps use pebbles to compact soil to close their burrow (Brockmann, 1985)
Emotions- Honey bees show a pessimistic judgment bias after stress in their expectation of a food reward (Bateson et al., 2011)- Fruit flies show a pessimistic judgment bias after stress in their expectation of a food reward vs. punishment (Deakin et al., 2018)
- Bumblebees show optimistic judgment biases after an unexpected food reward (Perry et al., 2016)
Navigation- Ants and bees learn places (von Frisch, 1967; Wehner, 2020)- Fruit flies and solitary wasps learn places (Tinbergen, 1932; Ofstad et al., 2011)
- Ants and bees learn routes between their nest and a goal (von Frisch, 1967; Wehner, 2020)- Dung beetles learn routes through celestial cues and wind compass, and path integration (Dacke et al., 2019)
- Honey bees and bumblebees develop traplines to exploit multiple feeding sites (Lihoreau et al., 2012; Buatois and Lihoreau, 2016)- Butterflies use traplines (Young and Montgomery, 2020)

Some examples of cognitive abilities reported in eusocial and non-eusocial insect species.

2.1. Individual recognition

Targeted social interactions, such as cooperation, may require specific recognition systems to identify different types of individuals in a group or population (Anderson and McShea, 2001; Wenseleers and van Zweden, 2017). As such, eusocial insects have evolved recognition systems enabling them to discriminate nestmates and non-nestmates (van Zweden and d'Ettorre, 2010), as well as individuals from specific castes within the colony (van Oystaeyen et al., 2014), through the use of odor cues. In some small colonies where dominance hierarchies are established to determine reproductive priority, insects also possess the ability to recognize individual nestmates. This is the case of colonies founded by several reproductive females, like for instance the paper wasps Polistes fuscatus that have unique facial coloration patterns (Tibbetts, 2002), or the ants Pachycondylla villosa that carry individual chemical signatures (D’Ettorre and Heinze, 2005). Though these recognition mechanisms have been implicated in the ecological success of insect societies (Jeanson and Weidenmüller, 2014) they are not specific to eusocial species. Theoretical models predict that the capacity to recognize group members should peak at intermediate levels of sociality and decrease as societies become larger (Gronenberg and Riveros, 2009), possibly due to the inability to manage so many social interactions and the risk of genetic conflicts in large colonies with different patrilines (Ratnieks et al., 2006). Moreover, if cognitive processes involved in individual recognition evolved due to the demands of recognizing reproductive competitors (Tibbetts, 2002), insect societies composed of highly related individuals should not possess such abilities. In line with these predictions, recent work shows males of the fruit fly Drosophila melanogaster, which form temporary aggregations on food sources for feeding and mating, appear to recognize other males with whom they compete for access to females (Yurkovic et al., 2006), although the cues involved are still unknown. In the solitary decorated cricket (Gryllodes sigillatus), individuals acquire the scent of their partners after mating. Female crickets, who recognize their own odor through self-referent phenotype matching favour pairings with males with unfamiliar odors to maximize their number of mating partners (Capodeanu-Nägler et al., 2014). While more studies are needed to better understand the evolution of individual recognition across insects, these observations in eusocial and non-eusocial species suggest individual recognition may be more closely related to reproductive competition and mate selection, rather than just social life.

2.2. Numerosity

Social life may require the ability for individuals to assess quantities and estimate the number of social partners or potential competitors in a group (Rios and Kraenkel, 2017). Several studies suggest eusocial honey bees (Apis mellifera) can count landmarks (Chittka and Geiger, 1995), recognize the number of objects in an image (Gross et al., 2009), perform basic operations (Howard et al., 2019), and understand the concept of zero (Howard et al., 2018, but see MaBouDi et al., 2021). Desert ants (Cataglyphis fortis) seem to count steps to evaluate distances during path integration (Wittlinger et al., 2006). However, whether these abilities are linked to sociality is not clear as many other behaviors could also require numerosity, like monitoring brood and forager populations and increasing resource collection to feed a colony, may simply result from individual variability in response threshold to environmental stimuli (i.e., the presence of pheromones, empty brood cells) not numeric competency (Page and Erber, 2002). Moreover, eusocial insect colonies can be so large so as to make recognizing and tracking all social interactions between conspecifics very unlikely (Gronenberg and Riveros, 2009). Thus, we would expect cognitive traits related to numerosity to evolve when monitoring potential rivals or mates is both feasible, given colony/population size, and is associated with increased reproductive fitness. Accordingly, some studies suggest non-eusocial insects have some sense of numerosity. For instance, males of the gregarious beetle Tenebrio molitor are capable of adjusting the intensity of their mate guarding strategy according to the number of males encountered before mating (Carazo et al., 2009). In the ladybug Adalia bipunctata females reduce egg laying according to the number of larvae from other females already encountered (Hemptinne et al., 1992). These observations, though relatively rare, indicate that insect numerosity may be more closely related to the likelihood of encountering competitors and mates than just group size.

2.3. Non-elemental learning

Living in a group may also multiply the need for animals to develop different types of associative learning. As more individuals are encountered, more types of interactions, relationships, and common experiences may have to be stored in memories (Bond et al., 2003). Honey bees (A. mellifera), that live in colonies with thousands of workers, can learn many elemental associations between a stimulus and a reward, but also more sophisticated non-elemental associations involving ambiguous stimuli (Giurfa, 2013). For instance, bees can learn to respond to a reinforced odor A and not to a non-reinforced odor B, and then have to learn the opposite when stimulus contingencies are reversed (Boitard et al., 2015). Honey bees can also be trained to patterning problems, by associating a simple component (A or B) to a reward and the mixture (AB) to an absence of reward or vice versa (Devaud et al., 2015). So far, however all these experiments simulate learning in a foraging context, suggesting that this might be a stronger driver of associative learning than social life per se. Accordingly, the gregarious fruit fly D. melanogaster was recently shown to solve similar non-elemental cognitive operations such as reversal learning (Mancini et al., 2019) and negative patterning (Durrieu et al., 2020), a task related to foraging for food or oviposition sites. Given these observations in non-eusocial insects, it might well be that these types of learning are primarily required to find food or mating partners rather than just for navigating more social interactions.

2.4. Social learning

Animals can learn about their environment by observing conspecifics (Laland, 2004). Information provided by others often improves individual decisions and is believed to be a major advantage of social life (Bourke, 2011). Famously, honey bees share information about the location of abundant food sources in the form of a symbolic waggle dance (von Frisch, 1967). Eusocial bumblebees can learn flower preferences through visual observation of more experienced conspecifics (Leadbeater and Chittka, 2005; Worden and Papaj, 2005) and new foraging techniques, such as pulling ropes (Alem et al., 2016) or rolling balls (Loukola et al., 2017). Interestingly, the study of the mechanisms underpinning flower choice copying in bumblebees showed this behavior can emerge from associative learning mechanisms that are not different from non-social learning (Dawson et al., 2013). It has thus been argued that social learning is just a form of individual learning with a social cue (Galef, 1995), and the emergence of social learning should correlate with the cost of information acquisition, not a direct result of group living. Supporting that theory, gregarious fruit flies D. melanogaster can also learn from their conspecifics. Females copy the choices of others for oviposition sites (Battesti et al., 2012) and mating partners (Danchin et al., 2018). Solitary wood crickets Nemobius sylvestris learn to hide after being in contact with conspecifics maintained under stressful conditions in the presence of spiders (Coolen et al., 2005). Thus, social learning and may employ associative mechanisms of non social learning can be observed in solitary species.

2.5. Tool use

In social groups with overlapping generations of individuals, social learning can lead to the emergence and cultural transmission of complex behaviors, like tool use (Shumaker et al., 2011). Such transmission is a form of heritable adaptation that is likely a key benefit of social life (Danchin et al., 2004). Several phenomena akin to tool use have been reported in eusocial insects (Pierce, 1986). For instance, workers of Pogonomyrmex and Aphaenogaster ant colonies occasionally use soil debris to transport liquid food that they could not otherwise bring back to their nests (Maak et al., 2020). However, examples of tool-users are also found in non-eusocial species. Antlion larvae throw sand to make their prey fall into the bottom of its pit (Oguma, 1930). African tree crickets pierce holes in leaves to create baffles and amplify their mating calls (Prozesky-Schulze et al., 1975). Solitary wasps use pebbles to compact the soil and close their burrows (Brockmann, 1985). Future research would benefit from identifying the specific learning mechanisms (i.e., social learning or trial and error) involved in tool use in these later examples remains unclear (Kenward et al., 2005). Once again, these abilities appear to be linked to foraging, sexual competition and defense, rather than the degree of sociality (Weir and Kacelnik, 2006).

2.6. Emotions

Many social interactions are regulated by emotional displays that enable communication between group members (van Kleef, 2009). In principle, being in a specific emotional state can improve responses to threats or social interactions and lead to more efficient collective responses. In this context, emotional states are elicited by rewarding and punishing goals, whereby rewards are stimuli that animals work to acquire and punishments are stimuli that they work to avoid Rolls (2014). Studies using judgment bias paradigms (Baciadonna and McElligott, 2015) indicate eusocial insects show emotional-like states. For instance, honey bees (A. mellifera) gently shaken just before the experiment showed pessimistic biases when faced the problem of classifying an ambiguous odor stimulus (Bateson et al., 2011) whereas bumblebees (Bombus terrestris) that received an unexpected reward showed consistent optimistic biases in a visual judgment task (Perry et al., 2016). However, similar emotional-like states were also recently reported in non-eusocial insects, such as in the gregarious fruit fly D. melanogaster where shaken flies showed a pessimistic bias in an ambiguous odor binary choice task, while control flies did not (Deakin et al., 2018). Again, this observation in non-eusocial fruit flies suggests emotional display is not specific to sociality. In fact, it is likely that these emotional states are triggered by basal neurohormonal mechanisms common across insect species (i.e., releases of biogenic amines) and increase efficiency of decision making in many contexts independent of sociality, such as foraging or predator avoidance.

2.7. Elaborate navigation

Social animals must intensely engage in foraging in order to gather and store large amounts of foods for their progeny. For central place foragers, this requires precise spatial memories and efficient navigation (Collett et al., 2013). Accordingly, eusocial insects are notoriously skilled navigators, learning near optimal routes between their colony nest and specific places based on cues in the local environment and on the panorama (Wehner, 2020). Honey bees and bumblebees can travel several kilometers to visit hundreds of flowers looking for nectar and pollen, and repeat these foraging trips over several days or weeks (von Frisch, 1967). When these resources are patchily distributed in space, foragers tend to develop multi-destination routes (i.e., traplines) by which individuals visit plant patches in stable sequences using the shortest possible path linking all these places (Lihoreau et al., 2012b; Buatois and Lihoreau, 2016). Strikingly however, many non-eusocial insects, including non-central place foraging species, are also excellent at these spatial cognitive tasks. In fact, the seminal experiment demonstrating place learning in insects were conducted in the non-eusocial wasps (Philanthus triangulum) in which moving the visual landmarks surrounding the nest of the wasp triggered search around landmarks (Tinbergen, 1932). Fruit flies D. melanogaster also develop visual place memories when forced to find a hidden cool tile in a warm arena based on patterns displayed on the panorama (Ofstad et al., 2011). Various butterflies have been observed developing traplines between distant plants (Young and Montgomery, 2020). Dung beetles use environmental cues to navigate in a straight line away from the dung pile as fast as possible (Dacke et al., 2019). Spatial cognition skills supporting efficient navigation are therefore not exclusive to eusocial insects and appear to rely more on ecological factors such as the variability of food sources in time and space rather than sociality per se.

3. Concluding remarks

Many cognitive abilities once thought to be required for social interactions and unique to eusocial insects are also being discovered in non-eusocial species (see summary in Table 1). This suggests factors related to foraging ecology, mate selection, reproductive competition, and defense not just sociality may give rise to the rich and elaborated cognitive capabilities found in insects. This also emphasizes the need for further theoretical and experimental research to clarify the relationship between ecology, sociality, and cognition in this group (Lihoreau et al., 2019). We argue this knowledge gap can be filled by overcoming methodological and conceptual challenges.

Foremost among these challenges is a scarcity of model organisms which limits our ability to assess how ecological and life history traits impact cognitive evolution. The large volume of insect behavioral and cognitive research is almost entirely based on highly eusocial ants, bees, and wasps. This is not surprising as these insects are easily maintained in both laboratory and natural environments, display a diversity of cognitive and neuroanatomical traits and have a well-established history of assays used to quantify cognitive variability (Giurfa, 2013). While there have been attempts to compare cognitive abilities across closely related, ecologically distinct, eusocial species (Sheehan and Tibbetts, 2011; Tait et al., 2021), examples are still rare and limited in their power due to the small number of species and phylogenetic groups usually considered in these studies (Harvey and Purvis, 1991). There are, however, other species of eusocial and non-eusocial insects, each with a unique ecology and life history but with very little information regarding their cognitive traits. Comparative analyses investigating such understudied insect clades could go a long way to improving our understanding of the role of ecology and sociality in the evolution of insect cognition, especially given the noticeable taxonomic bias revealed by our review of the literature (Figure 1). The network map of keywords extracted from the Web of Science (titles and abstracts) illustrates research biases for behavioral and learning in bees and ants (orange cluster), in contrast to a functional molecular bias in Drosophila studies (blue cluster).

Figure 1

Another difficulty lies in the unverified assumption that larger behavioral repertoires require larger brains (Godfrey and Gronenberg, 2019). Many fundamental changes in the complexity of a nervous system may not result in measurable volumetric differences, and novel behavior may emerge from minimal rewiring of existing neurons (Chittka and Niven, 2009). Therefore, recent studies point towards a need to consider a new framework for insect comparative cognition, including a comprehensive discussion on their ecology beyond their social status (Lihoreau et al., 2012a; O'Donnell et al., 2015; Farris, 2016; Simons and Tibbetts, 2019). If we want to move from describing cognitive feats in insect models to understand why and how these cognitive abilities have evolved across insects in general, we need to develop more systematic comparative analyses of species with contrasted ecologies (including foraging, mating, and social interactions) and detailed anotomo-functional measures of their brains. It is only by identifying cognitive operations that are specific to social life, the neural circuits they involve, and the ecology and life history of the species, that the social brain hypothesis and its derivatives, as they are currently framed, can be tested and refined.

Funding

This work was funded by grants from the Alexander von Humboldt Foundation (1223114) to LAP, the European Molecular Biology Organization (EMBO153-2022) to CT, and the European Commission (FEDER ECONECT MP0021763, ERC Cog BEE-MOVE GA101002644) to ML.

Publisher’s note

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Supplementary material

The Supplementary material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fevo.2022.1001045/full#supplementary-material

Statements

Author contributions

All authors listed have made a substantial, direct, and intellectual contribution to the work and approved it for publication.

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Summary

Keywords

social brain hypothesis, insects, social evolution, comparative cognition, foraging, mate competition

Citation

Poissonnier L-A, Tait C and Lihoreau M (2023) What is really social about social insect cognition?. Front. Ecol. Evol. 10:1001045. doi: 10.3389/fevo.2022.1001045

Received

22 July 2022

Accepted

14 December 2022

Published

06 January 2023

Volume

10 - 2022

Edited by

Simon M. Tierney, Western Sydney University, Australia

Reviewed by

Andre Riveros, Universidad del Rosario, Colombia

Updates

Copyright

*Correspondence: Mathieu Lihoreau,

†ORCID: Laure-Anne Poissonnier https://orcid.org/0000-0003-2264-6964

Catherine Tait https://orcid.org/0000-0002-3642-082X

Mathieu Lihoreau https://orcid.org/0000-0002-2463-2040

This article was submitted to Social Evolution, a section of the journal Frontiers in Ecology and Evolution

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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

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