Enhancing PQQ Production in Acinetobacter calcoaceticus through Uniform Design and Support Vector Regression

Yu-Han Li^1,2Su-Hang Yao^1,2Yan Author Zhou³Xiu-Lan He⁴Zheming Yuan⁵Qiulong Hu^1,2Cheng-Wen Shen^1,2*Xin Li^3*Yuan Chen^1,6*

• ¹Hunan Agricultural University, Changsha, China
• ²National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, Hunan Province, China
• ³Hunan Institute of Microbiology, Changsha, China
• ⁴Graduate School of Hunan University, Long Ping Branch, Changsha, Hunan Province, China
• ⁵Yuelu Mountain Laboratory of Hunan Province, Changsha, China
• ⁶Hunan Provincial Engineering and Technology Research Center for Agricultural Big Data Analysis and Decision-Making, Hunan Agricultural University, Changsha, Hunan Province, China

A novel machine learning-assisted approach for formula optimization, termed UD-SVR, is introduced by combining uniform design with support vector regression. This method was employed to optimize both the formulation and fermentation conditions for pyrroloquinoline quinone (PQQ) production by Acinetobacter calcoaceticus. In just two rounds of 66 experimental treatments, UD-SVR effectively optimized a formulation involving eight factors at the shake-out level scale, enhancing PQQ production from 43.65 mg/L to 73.40 mg/L-an impressive 68.15% increase. Notably, the optimized formulation is also cost-effective, featuring minimized consumption of pivotal elements like carbon and nitrogen sources. The machine learning-supported UD-SVR method presents an inclusive resolution for optimizing experimental designs and analyses in multi-factor, multi-level formulations, characterized by robust guidance, lucid interpretability, and heightened efficiency in optimization.