Impact of primary cancer history and molecular landscape in therapy-related myeloid neoplasms

Abstract

Background:

Therapy-related myeloid neoplasms (t-MN) are aggressive hematologic malignancies with poor prognosis and high-risk clinical features. Recent advances have highlighted the role of molecular data in refining prognostic models. This study aims to analyze a monocentric cohort of t-MN patients, focusing on the clinical and prognostic impact of prior malignancies and their associated molecular landscape.

Methods:

A retrospective analysis was conducted on 61 patients diagnosed with t-MN from an Oncology Hospital and referred to a hematology Unit. Diagnoses were based on established criteria for therapy-related myelodysplastic syndrome (t-MDS) and therapy-related acute myeloid leukemia (t-AML), with a history of prior exposure to cytotoxic therapy. Cytogenetic and molecular analyses supported the diagnoses. Risk stratification was performed using the revised International Prognostic Scoring System (IPSS-R) and molecular IPSS (IPSS-M) for t-MDS and the 2022 European LeukemiaNet (ELN) classification for t-AML.

Results:

Overall, 61 patients with t-MN were diagnosed: 38 (62.3%) with t-MDS, and 23 (37.7%) with t-AML. The median latency from primary cancer to t-MN diagnosis was 5.8 years (IQR: 2.6–12.5). Risk stratification identified 63.2% of t-MDS cases as IPSS-R very-low to intermediate risk, while 57.9% were reclassified as IPSS-M moderate-high to very high risk. Patients with prior hematologic cancer showed a greater tendency toward higher IPSS-R (p=0.021) and IPSS-M (p=0.015) risk compared to solid cancer. The IPSS-M, more accurately than R-IPSS, demonstrated predictive value for survival in both univariate and multivariate analyses and effectively predicted leukemic progression in t-MDS. TP53-mutated cases were more prevalent in patients with prior hematologic cancer (p=0.043) and associated with longer latency (8.2 years) compared to TP53 wild type (6.1 years, p=0.044). Allogeneic transplantation proved beneficial, significantly improving survival outcomes in eligible t-MDS and t-AML patients.

Conclusions:

t-MN exhibits distinct clinical and molecular profiles according to prior malignancy type. Intriguingly, our analysis reveals a distinct latency pattern in TP53-mutated cases, suggesting unique leukemogenic dynamics. Moreover, IPSS-M proved highly accurate in predicting t-MDS survival. Integrating molecular data into prognostic models enhances risk stratification and informs therapeutic strategies, potentially improving outcomes for t-MN patients. Further studies are needed to validate these findings and refine tailored treatment approaches.

1 Introduction

Therapy-related myeloid neoplasms (t-MN), comprised of therapy-related myelodysplastic syndromes (t-MDS), acute myeloid leukemia (t-AML), and myelodysplastic/myeloproliferative neoplasms (t-MDS/MPN), represent a rare but severe complication of cytotoxic treatments used for both malignant and non-malignant diseases (1, 2). While these treatments have substantially improved survival rates in cancer patients, they are also associated with an increased risk of secondary hematologic malignancies (3).

Cytogenetic and molecular profiling have revealed significant differences between t-MN and de novo MN. Specifically, therapy-related cases are characterized by a higher prevalence of high-risk cytogenetic abnormalities, including complex karyotypes (4, 5). In addition, next-generation sequencing (NGS) has revealed a mutational profile that, while largely shared with de novo cases, demonstrates a higher prevalence of mutations in adverse prognostic genes such as TP53, SETBP1, and SRSF2, and a lower incidence of mutations in genes like NPM1, FLT3, and IDH1/2 (6).

Recent advancements have integrated molecular data into classification and prognostic models for both t-MDS and t-AML. In t-MDS, a major achievement has been the development of the molecular International Prognostic Scoring System (IPSS-M), which incorporates molecular information and the revised IPSS (IPSS-R). The original study included 8% of t-MDS cases, which were more frequently categorized as IPSS-M high/very high risk but exhibited comparable outcomes to de novo cases within each risk category (7). Similarly, t-AML has not consistently emerged as an independent risk factor for survival in some studies. In contrast, others report worse outcomes in intermediate-high risk categories, with no difference in favorable risk compared to de novo AML (5, 8).

Historically, the prognosis of t-MN has been poor, with a 5-year survival rate of approximately 10% (9). This outcome is primarily influenced by factors such as advanced age, a higher burden of comorbidities, and the cumulative toxicities of prior cytotoxic therapies (10). To date, molecular analysis has been evaluated, providing prognostic insights and highlighting significant differences based on the latency from the primary malignancy (4). However, limited data are available regarding the molecular profile in relation to the primary cancer.

This study investigates the clinical and molecular characteristics of a monocentric cohort of t-MN patients from an Oncology Hospital referred to the Hematology Unit, focusing on the prognostic implications of prior malignancy types and their associated molecular profiles. These findings may enhance current risk assessment models and guide the development of personalized therapeutic strategies, ultimately improving patient outcomes.

2 Materials and methods

2.1 Study population

We performed a retrospective analysis of 61 patients diagnosed with t-MN and treated at our institution between January 2009 and November 2024. Clinical data were retrieved from medical records. The diagnosis of t-MN was established based on the recognized criteria for MDS or AML, combined with a documented history of exposure to cytotoxic therapy for unrelated malignancies (2). Patients with MN and a history of prior malignancy were excluded if they had not received chemotherapy or radiotherapy, immunotherapy, hormone therapy, target therapy, or if they had undergone surgery alone. Bone marrow evaluations were conducted using cytology, histology, and flow cytometry. The general clinical condition of patients was assessed using the Eastern Cooperative Oncology Group (ECOG) performance status (PS) (11). For t-MDS, risk classification was based on both the IPSS-R (12) and IPSS-M (7). For t-AML, risk stratification followed the 2022 ELN guidelines (13). The SIE/SIES/GITMO criteria were retrospectively applied to categorize patients as fit or unfit for intensive chemotherapy (14).

2.2 Cytogenetic and genetic analysis

Chromosomal banding was performed using standard techniques, with karyotypes described according to the International System for Human Cytogenetic Nomenclature (ISCN). Mutational analysis of FLT3-ITD, RUNX1::RUNX1T1, CBF::MYH11, PML::RARA, WT1, NPM1, and BCR::ABL1 was conducted using real-time PCR (RT-PCR).

Targeted NGS was performed to analyze 40 genes frequently mutated in myeloid neoplasms. Genomic DNA extracted from peripheral blood or bone marrow was processed using the Oncomine Myeloid Research panel (Thermo Fisher Scientific) and sequenced on the Ion Torrent GeneStudio™ S5 system with a targeted coverage depth exceeding 300x. Data analysis was conducted using Torrent Suite and Ion Reporter™ Software, with reads aligned to the hg19 reference genome. Variants were reported if the variant allele frequency (VAF) was ≥5% with ≥300x coverage or ≥2% for hotspot mutations with ≥100x coverage. Only pathogenic, likely pathogenic, or variants of uncertain significance were reported, adhering to HGVS nomenclature guidelines (15). Polymorphisms and benign variants were excluded from the report.

2.3 Statistical analysis

Continuous variables were reported as medians with interquartile ranges (IQR), and categorical variables as frequencies and percentages. Comparisons between groups were conducted using Chi-squared tests for nominal variables, Fisher’s exact test, and the Wilcoxon-Mann-Whitney test for non-parametric data. Logistic regression was employed to calculate odds ratios (ORs) with 95% confidence intervals (95% CI). Overall survival (OS) was defined as the time from t-MN diagnosis to death or last follow-up, and progression-free survival (PFS) was defined as the time from t-MDS diagnosis to diagnosis of leukemic progression. Kaplan-Meier curves were used for survival analysis, with differences assessed via the Log-Rank test. Hazard ratios (HR) with 95% CI for survival-associated factors were calculated using univariate and multivariate Cox proportional hazards regression. A p-value < 0.05 was considered statistically significant. Statistical analyses were conducted using R software (R Core Team, 2021), version 4.1.2 (R Foundation for Statistical Computing, Vienna, Austria).

3 Results

3.1 Demographics and biological features of t-MN cohort

Patient clinical and biological characteristics are detailed in Table 1. Overall, the analysis included 61 patients with t-MN, comprising 38 patients (62.3%) diagnosed with t-MDS and 23 (37.7%) with t-AML. The median age at t-MN diagnosis was 69.9 years (IQR: 59.0–74.6). Notably, patients with t-MDS were significantly older than t-AML patients at t-MN diagnosis (p<0.001). The majority of patients exhibited PS ECOG of 0–1 (86.9%), with no significant difference between t-MDS and t-AML subgroups (p=0.784). At the time of t-MN diagnosis, 38 patients (48.2%) presented with at least one comorbidity, most frequently cardiovascular disease (46.4%). Comorbidity burden and blood count data were comparable between diagnostic subgroups, except for a significantly lower median platelet count observed in t-AML (p=0.033).

A prior history of solid and hematologic cancer was documented in 38 (62.3%) and in 23 (37.7%) patients, respectively. The spectrum of primary malignancies and previous cytotoxic therapies is detailed in Figure 1. Cytogenetic analysis was conducted in all enrolled patients (Supplementary Figure 1), revealing abnormalities in 37 cases (60.7%), including a complex karyotype in 11 (18%). Notably, complex karyotypes were more frequent in patients with prior hematologic malignancies than in those with solid tumors (p=0.049). No other significant cytogenetic differences were observed based on cancer history.

Figure 1

Overall, 31 (50.8%) also underwent NGS analysis (Supplementary Figure 1). Among the 23 patients with NGS-detected mutations, 12 (52.2%) harbored a single isolated variant, 7 (30.4%) exhibited two co-occurring mutations, and 4 (17.4%) carried three or more concurrent mutations. Subgroup analysis by primary cancer type showed no significant difference in the frequency of isolated versus co-occurring mutations (p=0.931). However, TP53 mutations were more prevalent in patients with hematologic malignancies (n=6) compared to those with solid tumors (n=1) (p=0.043). Notably, none of the TP53-mutated cases harbored DNMT3A, TET2, or ASXL1 (DTA) mutations. In contrast, DTA co-mutations with non-DTA genes were observed in 7 cases (30.4%), more commonly in patients with prior solid tumors than in those with hematologic malignancies, though this difference did not reach statistical significance (p=0.619).

The median latency from primary malignancy to t-MN was 5.8 years (IQR: 2.6–12.5), with no significant differences between t-MDS and t-AML subgroups (p=0.413). Similarly, latency did not differ significantly between patients with a prior history of solid tumors and those with hematologic malignancies (p=0.536). To further characterize latency patterns, we stratified patients into three groups based on previously published data (16): short latency (<1 year), intermediate latency (1–10 years), and long latency (>10 years) (Supplementary Figure 1). The majority of cases (n=38, 62.3%) exhibited intermediate latency, while 18 (29.5%) had long latency and 5 (8.2%) showed short latency. Patients with a prior hematologic malignancy were more likely to experience short latency compared to those with solid tumors (17.4% vs. 2.6%, p=0.0412). No significant differences emerged for intermediate (56.5% vs. 65.8%, p=0.556) or long latency (26.1% vs 31.6%, p=0.868). Notably, TP53 mutations, classified as either single-hit (n=3) or multi-hit (n=4) according to ICC criteria (1), have shown a longer median latency from primary disease compared to TP53 wild-type [8.2 years (IQR: 4.6–19.3) vs. 6.1 years (IQR: 1.9–17.0), p=0.044] (Figure 2). Conversely, no significant differences were observed between DTA-mutated cases compared to TP53 or SF3B1 mutated. No differences were instead recorded between normal and complex karyotype, -7/del(7q) and -5/del(5q) cohort (Figure 2).

Figure 2

3.2 Clinical features and risk stratification of t-MDS patients

According to the 2022 WHO classification, 12 patients (31.6%) were defined as MDS with low blasts (MDS-LB), 7 patients (18.4%) as MDS with increased blasts-1 (MDS-IB1), 6 patients (15.8%) as MDS with increased blasts-2 (MDS-IB2), 5 patients (13.2%) as MDS with TP53 mutation (TP53-MDS), 3 patients (7.9%) as MDS with del(5q) (MDS-5q), 3 patients (7.9%) as MDS with SF3B1 mutation, and one patient (2.6%) as MDS with fibrosis. According to the IPSS-R score, most patients were categorized as very-low, low, and intermediate risk (63.2%) (Figure 3). Nineteen patients (50.0%) were evaluable for the IPSS-M score; of these, 57.9% had an IPSS-M risk ranging from moderate-high to very high. Regarding the primary cancer diagnosis, 23 patients (60.5%) had a history of solid tumors, and 15 (39.5%) had a previous hematologic malignancy. The distribution of primary tumor types among t-MDS patients is shown in Figure 1. Notably, patients with a history of hematologic cancer were younger (p=0.003), had lower platelet counts (p=0.002), and had a higher blast percentage at diagnosis (p=0.021) compared to patients with a history of solid tumors (Table 2). Consequently, a greater tendency toward higher-risk IPSS-R (p=0.020) and IPSS-M (p=0.012) categories was observed in patients with a history of hematologic malignancy. However, the comparison of latency times was not significant (p=0.347).

Figure 3

3.3 Cytogenetic and molecular profile of the t-MDS cohort

Cytogenetic abnormalities (Figure 4A) were reported in 20 patients (52.6%). Of these, 11 patients (32.4%) had two concomitant abnormalities, and 8 patients (23.5%) had ≥3 abnormalities. According to cancer history, patients with prior hematologic neoplasm had a higher frequency of detectable cytogenetic abnormalities than those with a history of solid cancer (p=0.042). Nineteen patients (50%) underwent NGS analysis, and mutations were detected in 15 patients (78.9%). Among them, 10 patients (66.7%) had a normal karyotype. Overall, TP53, SF3B1, ASXL1 and TET2 were the most frequently identified abnormalities (Figure 4B). DTA mutations were found in 42.1% of patients, more frequently in the solid cancer group than in the hematologic cancer group, though not significantly (50.0% vs. 25.0%, p=0.349). In our cohort, DTA mutations did not significantly correlate with age (p=0.861). All four patients with TP53-MDS had a history of hematologic malignancy, and 75% had a complex karyotype. Additionally, younger patients were at higher risk of TP53 alterations [OR 0.87 (95% CI, 0.72-0.99), p=0.040].

Figure 4

3.4 Treatment and survival analysis in t-MDS patients

Overall, 26 patients (68.4%) received treatment for t-MDS, including recombinant erythropoietin in 14 patients (36.8%), hypomethylating agents (HMA) in 8 patients (21.1%), eltrombopag in two patients (5.3%), luspatercept and lenalidomide in one patient each. Among patients treated with HMA, a median of 2 cycles was administered, with more than 50% of patients receiving ≤3 treatment cycles. Three patients who received HMA were referred for HSCT after achieving complete response (CR), with a 4-year OS of 100% compared to 25% (95% CI, 0.1-0.6) for non-transplanted patients. Of the remaining patients, two died of leukemic progression, and two of infectious complications.

With a median follow-up of 10.6 months (IQR: 5.7–29.8), the 1-year OS was 74% (95% CI, 0.6–0.9) (Figure 5A). Stratified analysis by sex revealed a significantly lower survival in male patients (Figure 5B), with a 1-year OS of 56% (95% CI, 0.3-0.8) compared to 93% (95% CI, 0.8-0.9) in females (p=0.006). Patients with a history of hematologic cancer had a 1-year OS of 59% (95% CI, 0.3–0.9) compared to 82% (95% CI, 0.6–0.9) in those with prior solid tumors, though without statistical significance (p=0.130) (Figure 5C). Notably, a significantly lower 6-month OS was found also for TP53-MDS compared to TP53 wild-type MDS (p=0.025) (Figure 5D). Conversely, no significant differences were observed based on the DTA mutation status (Figure 5E).

Figure 5

3.5 Progression rates to t-AML and prognostic performance of risk score

Overall, 9 patients (23.7%) experienced progression to AML. At a median follow-up of 9.0 months (IQR: 3,5-29.8), the 1-year PFS was 79% (95% CI, 0.65-0.96). PFS was lower in patients with prior hematologic cancer compared to solid tumors [64% (95% CI, 0.4-0.8) vs 87% (0.7-0.9)], with a trend towards statistical significance (p=0.071). Expectedly, disease progression was associated with a dismal prognosis (Figure 5F). In univariate analysis (Table 3), female sex was associated with better survival, while disease progression was associated with worse outcomes. Additionally, both the IPSS-R (p=0.020) and IPSS-M (p=0.012) scores were predictive of survival. However, in multivariate analysis, only IPSS-M remained a predictive factor for survival (HR 2.9, 95% CI 1.06-8.4; p=0.038). Notably, no differences were found in terms of 6-month PFS between risk cohorts according to IPSS-R (p=0.320) (Figure 6A). At the same time, IPSS-M effectively predicted progression to AML among different risk classes (p=0.038) (Figure 6B).

Figure 6

3.6 Clinical and genetic features of t-AML

According to the ELN 2022 classification, three patients (13.0%) were considered at favorable risk, 6 patients (26.1%) at intermediate risk, and 11 patients (47.8%) at adverse risk. Three patients (13.0%) had a diagnosis of APL and were all considered at low risk according to Sanz’s risk score. A history of solid and hematologic cancer was reported in 15 (65.2%) and 8 patients (34.8%), respectively. No differences were observed between the two groups regarding sex, age, and fitness (Table 4). Additionally, median latency from primary cancer was shorter for patients with a history of hematologic tumors, although not statistically significant (p=0.227) (Table 4). The distribution of primary diagnoses and treatments received for the primary tumor are shown in Figure 1.

All patients underwent immunophenotypic analysis at the time of t-AML diagnosis. Specifically, except for the three APL patients, an APL-like phenotype (i.e., CD34^- and HLA-DR^-) was observed in 4 patients (17.4%). Cytogenetic analysis was positive for abnormalities in 17 patients (73.9%). Cytogenetic and molecular abnormalities are pictured in Figure 7. Furthermore, 21 patients (91.3%) underwent RT-PCR, which was positive for abnormalities in 11 cases (52.4%). WT1 abnormalities were the most common (23.8%). Twelve patients (52.2%) underwent NGS testing. The analysis, which tested positive in 9 cases, revealed abnormalities in 9 different genes, mainly involving TP53 (25.0%), ASXL1 (25.0%), and U2AF1 (16.7%).

Figure 7

3.7 Treatment and survival analysis in the t-AML cohort

Overall, 16 patients (69.6%) received intensive chemotherapy for t-AML, including CPX-351 in 10 patients (43.5%), standard 3 + 7 regimen in 3 patients (13.0%), 3 + 7+ Gemtuzumab ozogamicin (GO) in 2 patients (8.7%), and one patient received FLAG-IDA regimen. Four patients (17.4%) received non-intensive therapy with venetoclax and azacitidine. APL patients (n=3) received chemo-free therapy according to the APL0406 treatment protocol.

Nine patients (39.1%) were referred for HSCT after a median time of 5.8 months (IQR: 5.1–6.8) from diagnosis. Three patients were at low ELN risk, three at intermediate risk, and other three at adverse risk. Of transplant recipients, 5 patients (55.6%) had received induction therapy with CPX-351, 3 (33.3%) received standard 3 + 7 chemotherapy, and one patient received FLAG-IDA. At a median post-transplant follow-up of 21.8 months (IQR: 14.5–36.3), only one death was recorded due to disease relapse.

At a median follow-up of 13.5 months (IQR: 5.8–49.2), the 1-year OS of the entire cohort was 65% (95% CI, 0.4–0.9) (Figure 8A). No significant differences were observed between the ELN 2022 risk categories [low risk: 100% vs intermediate risk: 75% (95% CI, 0.6–0.8) vs adverse risk: 64% (95% CI, 0.5–0.8), p=0.623] or based on primary disease (Figure 8B). However, a significant difference emerged based on fitness criteria, with a 6-month OS of 50% (95% CI, 0.1–0.7) for unfit patients compared to 88% (95% CI, 0.7–0.9) for fit patients eligible for intensive therapy (p=0.0029) (Figure 8C). Moreover, increased survival rates were observed in transplant-recipients compared to non-transplanted patients (p=0.021) (Figure 8D).

Figure 8

4 Discussion

In this retrospective study, we aim to provide new insights into the clinical and prognostic features of t-MN. The demographics and distribution of primary diseases were similar to prior studies (4, 5), with breast cancer being the most frequent malignancy. Indeed, advances in breast cancer diagnosis and treatment have improved survival rates but have also increased the incidence of t-MN (17). Similarly, among hematologic primary diseases, lymphoproliferative neoplasms were the most prevalent, likely reflecting the extensive use of polychemotherapy and the resulting improvements in survival rates (18).

In patients with t-MDS, prior studies have highlighted the superior prognostic accuracy of the IPSS-M compared to the IPSS-R (9, 19). Notably, a higher prevalence of high-risk features is typically observed in t-MDS compared to de novo cases (20, 21). However, in our cohort, over 50% of patients were categorized as low to very low risk based on the IPSS-R, consistent with findings from more recent studies (22, 23). Conversely, the molecular score identified a higher proportion of high-risk patients. To our knowledge, this is the first study to specifically analyze the distribution of risk categories between the IPSS-R and IPSS-M in relation to the history of primary malignancy, also demonstrating a slight superiority of the molecular score over the standard one. Patients with prior hematologic cancer exhibited more adverse characteristics, including TP53 mutation, with a higher proportion classified as high to very high risk by IPSS-M, likely due to greater exposure to polychemotherapy, multiple lines of cytotoxic treatment, and high-risk genetic alterations. Notably, this is highlighted by the higher prevalence of karyotypic abnormalities and a history of hematologic malignancies compared to solid tumors. The additional value of molecular analysis becomes even more pronounced when assessing the risk of progression to AML. In our cohort, the progression rate of 26.5% was consistent with previous reports (20), yet the IPSS-R score surprisingly failed to discriminate this outcome adequately. In contrast, a clear prognostic difference emerged when applying the molecular score. Although not statistically significant, the higher progression rate observed in patients with a history of hematologic neoplasm likely reflects greater biological complexity compared to solid cancer.

An intriguing aspect of our analysis is the observation of an extended latency period in patients harboring TP53 mutations compared to their TP53 wild-type counterparts, consistent with recent findings by Hung et al. (24). Similar prolonged latencies have also been reported in other contexts, although not directly compared to wild-type cases (16, 25). At first glance, this observation may appear paradoxical, as TP53-mutated clones are generally associated with more rapid progression to advanced, chemo-resistant disease stages (26). However, the leukemogenic mechanisms driven by TP53 mutations appear to diverge from those of other mutational processes, reinforcing the notion of TP53-mutated myeloid neoplasms as a distinct biological entity.

Notably, TP53 mutations are enriched in clones lacking concurrent DTA mutations, suggesting an alternative leukemogenic trajectory. Indeed, the mutational landscape of t-MN may differ depending on the type of primary malignancy, potentially reflecting distinct selective pressures imposed by previous therapies. TP53-mutated clones are well-documented for their resistance to chemotherapy, which predominantly relies on DNA damage-induced apoptosis, and they are recognized as early drivers in the pathogenesis of MDS and AML (27, 28). Moreover, latency duration does not appear to differ significantly between single-hit and multi-hit TP53 mutations (25). In this context, alterations within the bone marrow microenvironment may play a critical role. TP53-mutated clones seem to exist at subclonal levels prior to cytotoxic therapy, insufficient to outcompete normal hematopoietic cells or initiate overt leukemia. However, these clones may acquire a selective advantage when therapy-induced microenvironmental changes create conditions favorable for their expansion (29).

The extended latency observed in TP53-mutated cases may thus reflect the temporal requirement for therapy-mediated remodeling of the bone marrow niche to establish an environment conducive to the proliferation of therapy-resistant TP53-mutated clones. However, the concept of “latency” requires further investigation, particularly in relation to clonal evolution, the role of the bone marrow microenvironment, and whether prolonged exposure to prior cytotoxic therapies acts as a critical trigger in the development of t-MN. It can be hypothesized that the cumulative effect of cytotoxic treatments in prior malignancies resembles the role of reactive oxygen species (ROS) in oxidative stress, where sustained exposure leads to DNA, mitochondrial, and protein damage, ultimately promoting genomic instability. A key limitation of our analysis is the combined evaluation of latency in both t-MDS and t-AML, which may mask the specific contributions of certain mutations to different stages of disease progression.

A crucial aspect of managing t-MN concerns the differing outcomes for t-AML and t-MDS. While survival rates for t-AML have shown modest improvement, likely due to the introduction of CPX-351, similar advancements have not been seen in t-MDS (30, 31). According to the IPSS-R, most t-MDS patients received supportive therapies, with only a small subset treated with HMAs. The high mortality in high-risk t-MDS patients without access to HSCT underscores the lack of effective curative options for this group. In our cohort, HSCT provided a significant prognostic benefit, with markedly improved outcomes in t-AML patients who underwent transplantation. The importance of patient selection was evident, with significant survival differences observed between fit and unfit patients based on the SIE/SIES/GITMO criteria, highlighting their crucial role in treatment decisions. All unfit patients received non-intensive therapy, while fit patients underwent intensive therapy, achieving complete response, which was present in all transplanted patients.

To note, our study has several limitations. First, the limited sample size and retrospective design introduce potential selection and analysis biases. Indeed, molecular data and molecular-based risk stratification were not available for all patients. Furthermore, although not the primary focus of the study, assessing the individual contribution of each chemotherapeutic agent was not feasible due to the widespread use of polychemotherapy regimens and multiple lines of therapy. More importantly, this limitation precluded evaluating how primary disease therapies affect cytogenetic and molecular abnormalities. Nevertheless, our study emphasizes the contribution of molecular analysis to improving the prognostic stratification of patients with t-MDS, particularly in predicting progression to AML, and highlights its critical role in clinical decision-making. Furthermore, there is an urgent need for appropriate therapeutic solutions for patients with t-MDS, for whom HSCT remains the only potential curative option.

5 Conclusions

This study provides a comprehensive analysis of the clinical and genetic characteristics of patients with t-MN, highlighting key differences between patients with a history of solid and hematologic tumors and emphasizing the importance of incorporating molecular analysis into the diagnostic and decision-making process. We also underscore the urgent need for effective therapeutic solutions for t-MDS patients, where HSCT remains the only curative option for eligible patients. We also stressed the importance of appropriate patient selection for intensive therapies in t-AML. Overall, these findings reinforce the need for a personalized treatment strategy based on clinical, cytogenetic, and molecular criteria to optimize outcomes in this complex and high-risk population.

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