ORIGINAL RESEARCH article
Front. Pediatr.
Sec. Pediatric Gastroenterology, Hepatology and Nutrition
Volume 13 - 2025 | doi: 10.3389/fped.2025.1421992
This article is part of the Research TopicGastrointestinal Infections in Pediatric PopulationsView all articles
Treatment response evaluation in an ex vivo model of E.coli infected central venous catheter system
Provisionally accepted
- University Children’s Hospital Basel, Basel, Switzerland
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Introduction: Despite all precautions, central line-associated bloodstream infections (CLABSIs) are inevitable, especially in children. Different treatment strategies exist for those situations. The aim of this study is to compare the different treatment strategies.Methods: In this study central venous catheters (CVC, Broviac® single lumen) were infected contaminated with E. coli in vitro. Different treatments (70% ethanol, ceftriaxone, TauroLock®) were applied and the effect was measured by isothermal microcalorimetry.Results: A rapid decrease in heat release corresponds to a rapid decrease in the number of living bacteria. Ceftriaxone had the quickest effect followed by ethanol in combination with ceftriaxone, ethanol, and TauroLock®.Discussion: Antibiotics must be based on patient risk factors, severity of infection and local resistance pattern, therefore it is difficult to publish general guidelines applying to all children. In this in vitro study, ceftriaxone demonstratedhad the most efficient effect the highest efficacy on the bacteria. Taurolodine locks are recommended for preventing CLABSI, but no data is available in regards using it for treatment: In this setting, it was efficient, as was ethanol. However, the bacteria used in this study, have not been exposed to antibiotics before -this is most likely in contrast to patients, who have a central venous catheter. Conclusion: Under in vitro conditions, systemic ceftriaxone is the most efficient and fastest treatment for an E. coli infected CVC. Elimination of bacteria was also reached with 70% ethanol and TauroLock®, but it needed more time.
Keywords: Central venous catheter, Infection, Treatment, antibiotics, microcalorimeter, Parenteral Nutrition
Received: 23 Apr 2024; Accepted: 16 May 2025.
Copyright: © 2025 Legeret, Huo, Holland-Cunz and Gros. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Corinne Legeret, University Children’s Hospital Basel, Basel, Switzerland
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